K22140

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K22140

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T2 seed (PO:0009010)
  • increased width (PATO:0000600)
    (Seed::Shape::wide)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K22140

Determination of introduced cDNA(s)

  • J033121A17 - Rice Full-length cDNAs - Original: J033121A17

    full reading [+] show sequence

    Nucleotide sequence: J033121A17 - full reading 
    GGCCGTGCCC GGTCGGATCT AATTAATTTA CCTCCTCCAC CTCCTCCTCC 
    TCCCCCCGAT CGGATCGGCC CGGCTCCTCC TCCTCCGCCG CCGCCACCGC 
    CGCCGCAGAT AGGAGAAGAT GGCCGCACTT GATACCTTCC TCTTTACCTC 
    GGAGTCTGTG AACGAGGGCC ACCCTGACAA GCTCTGCGAC CAAGTCTCAG 
    ATGCTGTGCT TGATGCCTGC CTCGCCGAGG ACCCTGACAG CAAGGTCGCT 
    TGTGAGACCT GCACCAAGAC AAACATGGTC ATGGTCTTTG GTGAGATCAC 
    CACCAAGGCT AACGTTGACT ATGAGAAGAT TGTCAGGGAG ACATGCCGTA 
    ACATCGGTTT TGTGTCAGCT GATGTCGGTC TCGATGCTGA CCACTGCAAG 
    GTGCTTGTGA ACATCGAGCA GCAGTCCCCT GACATTGCAC AGGGTGTGCA 
    CGGCCACTTC ACCAAGCGCC CTGAGGAGAT TGGTGCTGGT GACCAGGGAC 
    ACATGTTTGG ATATGCAACT GATGAGACCC CTGAGTTGAT GCCCCTCAGC 
    CATGTCCTTG CTACCAAGCT TGGCGCTCGT CTTACGGAGG TTCGCAAGAA 
    TGGGACCTGC GCATGGCTCA GGCCTGACGG GAAGACCCAA GTGACTGTTG 
    AGTACCGCAA TGAGAGCGGT GCCAGGGTCC CTGTCCGTGT CCACACCGTC 
    CTCATCTCTA CCCAGCATGA TGAGACAGTC ACCAACGATG AGATTGCTGC 
    TGACCTGAAG GAGCATGTCA TCAAGCCTGT CATTCCCGAG CAGTACCTTG 
    ATGAGAAGAC AATCTTCCAT CTTAACCCAT CTGGTCGCTT CGTCATTGGC 
    GGACCTCATG GTGATGCTGG TCTCACTGGC CGGAAGATCA TCATTGACAC 
    TTATGGTGGC TGGGGAGCTC ACGGTGGTGG TGCCTTCTCT GGCAAGGACC 
    CAACCAAGGT TGACCGCAGT GGAGCATACG TCGCAAGGCA AGCTGCCAAG 
    AGCATTGTTG CTAGTGGCCT TGCTCGCCGC TGCATTGTCC AAGTATCATA 
    CGCCATCGGT GTCCCAGAGC CACTGTCCGT ATTCGTCGAC ACATACGGCA 
    CTGGCAGGAT CCCTGACAAG GAGATCCTCA AGATTGTGAA GGAGAACTTC 
    GACTTCAGGC CTGGCATGAT CATCATCAAC CTTGACCTCA AGAAAGGCGG 
    CAACGGACGC TACCTCAAGA CGGCGGCTTA CGGTCACTTC GGAAGGGACG 
    ACCCAGACTT CACCTGGGAG GTGGTGAAGC CCCTCAAGTG GGAGAAGCCT 
    TCTGCCTAAA AGCTCCCTTT CGGAGGCTTT TGCTCTGTCC CATTATGGTG 
    TTTTGTTTCC TCGCTGCTCA GCATTGTGAT TCTTAACCTG CCCCCCGCTG 
    CCATTTATGC CCATGCACGC TACTTTCCTA ATAATAAGTA CTTATAAGGG 
    TATTGTGTTT GAATATTTTA CCTAGAGGAG GAGGAGGATT TGTTATCTGT 
    TATTGCTTAA GCTTATGTAA TGGTGAAAGA AGGAAAGATG CTACACTACT 
    TTGAGAAGAA GAAATGAAAC CTCCTATGTT TTGGTC

    Gene models with high sequence identity

    1. AT1G02500.2 [+] show detail - S-adenosylmethionine synthetase 1
      E-value: 0; Score: 714.00

     

    InterPro Scan Digest

    Program Description E-value
    superfamily S-adenosylmethionine synthetase 2.7e-62
    superfamily S-adenosylmethionine synthetase 7.2e-51
    superfamily S-adenosylmethionine synthetase 5.2e-45
    HMMTigr S-adenosylmethionine synthetase 3.2e-239
    HMMPfam S-adenosylmethionine synthetase 4.3e-70
    HMMPfam S-adenosylmethionine synthetase 3.9e-50
    HMMPfam S-adenosylmethionine synthetase 3.1e-43
    HAMAP S-adenosylmethionine synthetase 51.107
    PatternScan S-adenosylmethionine synthetase 0
    PatternScan S-adenosylmethionine synthetase 0
    Gene3D no description 2.4e-44
    Gene3D no description 4.8e-40
    Gene3D no description 3.1e-54
    HMMPIR S-adenosylmethionine synthetase 4e-235
    HMMPanther S-adenosylmethionine synthetase 4.8e-286

    See the detailed result >

    AT1G02500.2

    Model type
    Protein coding
    Short Description
    S-adenosylmethionine synthetase 1
    Curator Summary
    encodes a S-adenosylmethionine synthetase. SAM1 is regulated by protein S-nitrosylation. The covalent binding of nitric oxide (NO) to the Cys114 residue inhibits the enzyme activity.
    Computational Description
    S-adenosylmethionine synthetase 1 (SAM1); FUNCTIONS IN: methionine adenosyltransferase activity; INVOLVED IN: response to cadmium ion, response to salt stress, ethylene biosynthetic process, S-adenosylmethionine biosynthetic process; LOCATED IN: cell wall, plasma membrane, membrane; EXPRESSED IN: 30 plant structures; EXPRESSED DURING: 17 growth stages; CONTAINS InterPro DOMAIN/s: S-adenosylmethionine synthetase (InterPro:IPR002133), S-adenosylmethionine synthetase superfamily (InterPro:IPR022636), S-adenosylmethionine synthetase, N-terminal (InterPro:IPR022628), S-adenosylmethionine synthetase, C-terminal (InterPro:IPR022630), S-adenosylmethionine synthetase, conserved site (InterPro:IPR022631), S-adenosylmethionine synthetase, central domain (InterPro:IPR022629); BEST Arabidopsis thaliana protein match is: S-adenosylmethionine synthetase 2 (TAIR:AT4G01850.2); Has 10905 Blast hits to 10897 proteins in 2898 species: Archae - 12; Bacteria - 5497; Metazoa - 373; Fungi - 167; Plants - 706; Viruses - 1; Other Eukaryotes - 4149 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR