K22319

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K22319

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage
Phenotypes
T2 seed (PO:0009010)
  • increased width (PATO:0000600)
    (Seed::Shape::wide)
  • low saturation (PATO:0000328)
    (Seed::Color::pale)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K22319

Determination of introduced cDNA(s)

  • J013088A04 - Rice Full-length cDNAs - Original: J013088A04

    full reading [+] show sequence

    Nucleotide sequence: J013088A04 - full reading
    TGTTTCTGGC AGAATTGATT GGTGCAGTAT GAAGTTCTCA TCCTCACCAA 
    TATATGAAGC TAACAACAAA CATATGACAC ATTGCCATTC TTGTAAAGAT
    ATTGATTTAC TGCAGACAAA AGATGGTCCT TTCTGCAGGT GCATCCTTAA
    AAATTCCATT GTGTGTACCC CTCATAATAA CATCTTTTAT GTTATTAGTG
    GGTTTCTTGA CTTAGATGCA AATTCCTGTT TGCCTCAACA TGATGGGACT
    GCTGTTACCT ACAAAGATTA TTTTAAGACT TGGCATGGTC TTACTTTGAC
    TTTTGAAAAT CAGCCGCTCT TGGCTGGCAG TAAACATGTG AAAGTGCGGA
    ACTTCCTCCA CAACTGCTAC TCTAAAAAAG AAAAAGATCC TGGAAGCATT
    AACAACAAAG AAATGCCAAG AAGAATTTTC ACAGGAATCA TTAGAAACAC
    TTGGGGATTC TTTCCTCAAA TATGTTACAA CTCGGCACCT TTTTAGTGAA
    TACAGGCTTC AACATGAAGG CATATTAACA AAAATGAAGA AAAACTTGAT
    CTCCAATGCA GCTCTGTGCC AGCTGGCCTG CAGCAGTAAT CTTGTGGGTT
    ACATTCATGC TGAGGAGTTT AATCCAAGAG ATTGGATCAT CCCTTGCTTG
    GACTATGATG AGCGTGATAA CAAAAAAATT TCCTTTCTGG CACCCAATGG
    TATGTACAGC CAAAGGAAAA TGTCTATTAA AAGTAAGAGG ATTGCGGATT
    CAGTTGAGGC CTTAATTGGG GCTTACCTCA GTACTGCTGG GGAAAAGGCA
    GCATTTCTTT TGATGAAATC ATTAGGGATG AATATAGAAT TTCACACTGA
    AATTCCAGTT GAAAGGAAAA TTTCAATGAA AGCTGAAGAG TTCATTAATG
    TGAGGAGCTT AGAGGGAATG CTTGGTTACA AGTTCAATGA TTCTTTATTA
    TTGCTCGAAG CATTGACACA TGGCTCATAT CAGACTTCTG GCCCTACTTC
    ATGCTATCAG AGGCTCGAGT TTCTTGGAGA TGCTATTTTG GATCATCTTT
    TCACTGAATA TTACTACAGC AAATACCCTG ACTGTACTCC AGAATTGTTG
    ACAGATCTTA GATCTGCCTC TGTGAACAAC AATTGTTACG CGCATGCAGC
    TGTTAAATCA GGATTAAACA AGCATATCCT TCACTCATCA TCAGAACTGC
    ATAGGAAGAT GAGCTACTAC CTTGAGGAAT TTGGGCAGTC ATTTACAGGT
    CCATCTTATG GCTGGGAAGC GGGCATTGGT CTACCAAAGG TTCTCGGGGA
    TGTGATTGAA TCAATAGCTG GTGCCATTTA TCTCGACAGC AAGTGTGACA
    AGGAGGTTGT TTGGAGAAGC ATGAAGCGGC TATTGGAACC TCTTGCCACC
    CCCGAAACCA TCGAGCCTGA CCCGGTGAAA GGGCTGCAAG AATTTTGTGA
    TCGTAGATCA TTCAAAATAA CCTACGAAAA AAACCATGTT GATGGAGTCT
    CATCGGTCAT TGCTCGGGTG AAGGCTGGAG AGACTACATA CTCCGCAACA
    AAATCAGGTC CCTGCAAGTT GGTGGCGAAA AAGTTGGCAT CAAAGGCTGT
    GCTTAAAGAT CTGATTGCTG GTCACAAAGA TACAGAGGCT GCTGCAGTAT
    AGTGATACTT AACTATTTAA GCGTGCAACA GTAGCTAACA ATTGATATAG
    ATAGTTGCGC ACAATAGAAA AGTTTTGGTT GGATTCATAC ATGTAATTTG
    GAAATGCTCC TTTTTGTGTG CTCGCAAACG ATGCGTGTGA ACGGCCTCAT
    GAATTCTGGT AGGTTTTCAT GCTTCAAACT TACAAGTATC GCAATTTTGT
    AAATTACAAA TACGGGCATA GCACACCTGC CCATGCCATG CAGTAGTTTT
    TTTTTTCCTC GAAATAGTAG CATTAGTTGT ATATTACTCA TAGCATCAGA
    TTGTACAGAA AGCATGCCAG CAAAAACGAA AAAATGTACA GACAGCATTT
    TGTTCGCATC AAGGGAAAAC TTGTTTT

    Gene models with high sequence identity

    1. AT3G03300.3 [+] show detail - dicer-like 2
      E-value: 4e-91; Score: 332.00

     

    InterPro Scan Digest

    Program Description E-value
    Gene3D Ribonuclease III 7.5e-25
    Gene3D Ribonuclease III 2.3e-37
    HMMSmart Ribonuclease III 2.7e-22
    HMMSmart Ribonuclease III 2.5e-30
    HMMSmart Double-stranded RNA binding 0.0069
    ProfileScan Argonaute/Dicer protein, PAZ 13.157
    ProfileScan Double-stranded RNA binding 10.12
    ProfileScan Ribonuclease III 34.508
    ProfileScan Ribonuclease III 21.476
    HMMPfam Argonaute/Dicer protein, PAZ 3.4e-11
    HMMPfam Ribonuclease III 8.8e-24
    HMMPfam Ribonuclease III 4.3e-23
    PatternScan Ribonuclease III 0
    superfamily PAZ domain 3.6e-14
    superfamily Ribonuclease III 3.2e-40
    superfamily Ribonuclease III 2.2e-26
    HMMPanther DICER-1 3e-59
    HMMPanther HELICASE-RELATED 3e-59

    See the detailed result >

    AT3G03300.3

    Model type
    Protein coding
    Short Description
    dicer-like 2
    Curator Summary
    Encodes a Dicer-like protein that functions in the antiviral silencing response in turnip-crinkle virus-infected plants but not in TMV or CMV-strain-Y-infected plants. Involved in the production of ta-siRNAs. Partially antagonizes the production of miRNAs by DCL1. Substitutes for DCL4 to produce viral siRNA when DCL4 is missing or inhibited. Able to produce siRNAs but not miRNAs.
    Computational Description
    dicer-like 2 (DCL2); FUNCTIONS IN: in 7 functions; INVOLVED IN: defense response to virus, maintenance of DNA methylation, production of ta-siRNAs involved in RNA interference; LOCATED IN: intracellular; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: DNA/RNA helicase, DEAD/DEAH box type, N-terminal (InterPro:IPR011545), Double-stranded RNA-binding (InterPro:IPR001159), Argonaute/Dicer protein, PAZ (InterPro:IPR003100), Ribonuclease III (InterPro:IPR000999), DEAD-like helicase, N-terminal (InterPro:IPR014001), DNA/RNA helicase, C-terminal (InterPro:IPR001650), Dicer double-stranded RNA-binding fold (InterPro:IPR005034), Helicase, superfamily 1/2, ATP-binding domain (InterPro:IPR014021); BEST Arabidopsis thaliana protein match is: dicer-like 4 (TAIR:AT5G20320.1).
    Link
    InterPro Scan - TAIR