K22411

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K22411

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T1 rosette leaf (PO:0000014)
  • increased length (PATO:0000573)
    (Rosette leaf::Shape::long)
  • increased width (PATO:0000600)
    (Rosette leaf::Shape::wide)
  • dark green (PATO:0001249)
    (Rosette leaf::Color::dark)
  • present in fewer numbers in organism (PATO:0001997)
    (Rosette leaf::Number::few)
sporophyte vegetative stage (PO:0007134)
  • decreased speed (PATO:0000304)
    (Adult plant::Growth::slow)
seed (PO:0009010)
  • present in fewer numbers in organism (PATO:0001997)
    (Seed::Number::few)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K22411

Determination of introduced cDNA(s)

  • J013026J17 - Rice Full-length cDNAs - Original: J013026J17

    full reading [+] show sequence

    Nucleotide sequence: J013026J17 - full reading 
    GGCGCTGGCC GTCGCAAGGC CGCCGCATAC TCTTCTCCCC TCTTTGACTC 
    GTTTTGCGCC CAAGTCGATC CCCAACGTCT CCTCCTCGCT GGTTCGCCCG 
    CCGCGCCGCG CCGATCTCCA TCCGTTCCAC GAGCAGCGGT GACCGGGTGC 
    TGTCGTCCTG CTCCCCGCTT CCCTTCCGGC TCCGATTTGA GGCGCTAAAG 
    TTTCCATTCC GTGTTACGAA AAGCTGCATT AGGACATGGA CATGGCATTG 
    CCAATAGTGA ATGCCACCGC AGCGGTGCTT GCTCGTGTCT CAGCTGCATT 
    CAATGCCCCT TTTGCCCGCG CAGTTGTCTT TGGGGTTCAT ATCGATGGGC 
    ACCTGGTTGT TGAAGGGCTC CTTATAGCGG TCATAGTGTT TCAGCTTTCT 
    AGGAAGAGCT ACAAACCGCC CAAGAAGCCA CTCAGTGAAA AGGAGATTGA 
    CGAGCTATGT GATGAATGGG AGCCAGAACC TCTGTGTCCT CCGATCAAGG 
    ATGGGGCCCG AATTGACACA CCAATGTTGG AAAGTGCTGC TGCTCCACAT 
    ACAACCATTG ATGGGAAAGA AGTTATAAAC TTTGCATCAG CAAATTATCT 
    TGGTTTGATT GGCAATGAGA AGATTATTGA TTCTTGTGTT GGTTCACTGG 
    AGAAATATGG TGTTGGTTCT TGTGGTCCAC GTGGCTTTTA TGGAACAATT 
    GATGTCCACC TTGACTGTGA GGCAAAAATA GCGAAGTTTC TTGGAACTCC 
    AGACTCAATT CTGTATTCAT ATGGGATTTC TACAATATTT AGTGTGATAC 
    CTGCCTTTTG TAAGAAAGGA GACATCATAG TTGCTGATGA AGGTGTGCAC 
    TGGGCAGTGC AAAATGGTCT TCATCTGTCA AGAAGCACTG TTGTTTATTT 
    TAAACACAAT GATATGGCTT CACTTGCAAA CACTCTGGAA AAACTTACAC 
    GTGGAAATAA ACGAGCAGAA AAGATCAGAC GATACATTGT TGTAGAATCT 
    ATCTACCAGA ATTCTGGTCA AATTGCTCCC TTGGATGAGA TTGTCAGATT 
    GAAGGAGAAA TATCGATTCC GTGTTATTCT GGAGGAAAGC CATTCTTTTG 
    GTGTGCTTGG CCAGTCTGGA CGAGGCCTTG CTGAACATTA TGGAGTTCCA 
    ATTGACAAAA TTGATATTAT CACTGCTGGA ATGGGGAATG CATTAGCTAC 
    TGATGGTGGC TTTTGTACAG GAAGTGTTAG AGTTGTTGAT CATCAGCGTC 
    TAAGCAGTTC TGGCTATGTT TTCTCGGCTT CTCTGCCACC TTATCTTGCC 
    AGCGCTGCGG TTTCTGCTGT CAGCTACCTG GAGGGGAACC CGTCAGTTCT 
    TGCAGACCTG AGGAGCAACA TTTCTTTCCT GCATAAAGAA CTGTCAGGTA 
    CTCCAGGACT TGAAATTTCC AGCCATGTTC TGTCACCTAT CGTTTTCCTC 
    AAACTGAAGA AATCGACGGG TTCATCAAAC ACCGATATAG ATCTTCTTGA 
    AACTATTGCT GAGAGGGTAT TGAAGGAAGA TTCAGTTTTT ATCGTGGCGT 
    CAAAGCGGTC ACCTTTGGAT AGGTGCAAAC TTCCCGTCGG AATACGCCTC 
    TTCATGTCAG CCGGCCACAC CGATTCAGAC ATCTCCAAAG TGTCCTCATC 
    CTTGAAGCGG GTCTCAGCAT CAGTTCTGTC AGATTACATC TGATCCCCTT 
    TCTCTACGAG CTGTGCACTA ATCTCTGTGT GCATGTTTGG TTAATGTACA 
    TAACCTGTAC ATTTGTAGGT TCCGATGATT CCCGTTGCCG CTCATACTTG 
    CAAACACTTC GAAAAATAGA CGTAGAACAG TGAAATTAGG TGTGTTATGT 
    ATCGTTCGGT CTTCTGGTAG TGGTTTCCCG GTAGAATTCA TTCTATATCT 
    TTAATTTTGT CCTCTATTCA TCACTTTTAT CATTTTGGCT TC

    Gene models with high sequence identity

    1. AT4G36480.2 [+] show detail - long-chain base1
      E-value: 5e-180; Score: 628.00

     

    InterPro Scan Digest

    Program Description E-value
    Gene3D Pyridoxal phosphate-dependent transferase, major region, subdomain 1 3e-71
    HMMPfam Aminotransferase, class I/II 3e-40
    superfamily Pyridoxal phosphate-dependent transferase, major domain 2.7e-70
    Coil coiled-coil 0
    HMMPanther SERINE PALMITOYLTRANSFERASE I 1.4e-226
    HMMPanther CLASS II AMINOTRANSFERASE/8-AMINO-7-OXONONANO 1.4e-226

    See the detailed result >

    AT4G36480.2

    Model type
    Protein coding
    Short Description
    long-chain base1
    Curator Summary
    Encodes the LCB1 subunit of serine palmitoyltransferase. Together with the LCB2 subunit, forms a functional serine palmitoyltransferase complex, which catalyzes the first reaction of sphingolipid biosynthesis. Knockout of LCB1 was embryo lethal. Partial suppression of LCB1 expression led to smaller plants due to reduced cell expansion.
    Computational Description
    long-chain base1 (LCB1); FUNCTIONS IN: protein binding, serine C-palmitoyltransferase activity; INVOLVED IN: cell growth, sphingolipid biosynthetic process; LOCATED IN: endoplasmic reticulum; EXPRESSED IN: 20 plant structures; EXPRESSED DURING: 12 growth stages; CONTAINS InterPro DOMAIN/s: Pyridoxal phosphate-dependent transferase, major domain (InterPro:IPR015424), Aminotransferase, class I/classII (InterPro:IPR004839), Pyridoxal phosphate-dependent transferase, major region, subdomain 1 (InterPro:IPR015421); BEST Arabidopsis thaliana protein match is: long chain base2 (TAIR:AT5G23670.2); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR