K22429

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Phenotypes

Morphological phenotypes

Generation	Anatomical entity /structure development stage	Phenotypes
T2	seed (PO:0009010)	increased width (PATO:0000600) (Seed::Shape::wide)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K22429

Determination of introduced cDNA(s)

J033072F08 - Rice Full-length cDNAs - Original: J033072F08

full reading [+] show sequence

GGCGAAATCC CTAAACCCTA GCCGCCGCCG CCGCCCCCGC CCCGGCATCG 
GCGACGACTC CGACCTCCAG AAGGACCTGG ACGAGCCGCG CCGCTCCTGC 
ATCCTCTCCG GGAACGACCC CGACGCCGCG GTCGCGCAGG TCTCCGCCAG 
CCTCGCCGCC GGCACATCAG GGGCGGGGGG CGGCGAAGAA TGGCTTTGTC 
CGATGATGAC GAGGAGGAGG AGGACGATGG CTTTGGCGCT CGTCCGTAGC 
ATCCGTGAGA ATCTCCTCAA CAAGGCCTCT TGCCCTCCTC ACCCGATCTG 
CGTCTGGCCG CCCTCCGACT TGGAGGAGGA CGACGACAAC GACCTCGAGA 
CGCTCCGTGC GATACAGCAC CCACTTCTCG CACTACCATT CCGGTACCTC 
CAGTGGGTCA GAGACAAATA CAAAAACTGA GGAAATCTTT TTCGAAATAG 
ACTTGATGAA GAATTTGATG TGGATAAGCA AAATAAAGAG GCAATTACCC 
CAATTGGGTT TCCTAAAGCT GCCTGCTGTT AGTGGATGCT CTTAAGAAGA 
ACAGAGCATG CCAGAAGTTC ATTAGAAGGT AGATTACAAT TCAAGCGAAA 
TTGAAGAAAA TAAGAATCTT AATCTTAAGG ATCATGTAAA ATGCCTTTTG 
GATTATCAGT TAAGCCGCAG AAAAGAATTT GGCAAAATTT TGTGCCAAAA 
TGAGGATCAT TATGTTAGAT TGTTTTCCTC TCAAAAGCCA TGTGCACAAT 
CAGCGAAGGA CACAATTCTA AGGATCTATG TTGCCCACCG GAAAACATTA 
AGGGGCGCAC CCTGGGGCGA TGGAGGGAGG TGCCACGTCG GCGATTTGCT 
ACAGTCCTAC AGTGCTCGGG GTGGTCGTTT TGCAAAAATA ACCCTCCGGT 
TTGCTGGTAA TTCGAAGATG CAACTAGGAA CAATTTTCAT CAGTATGCTC 
AAGATCCAAA TGACCGAAAT ACTGGACAGA TGTACCATGG ATCCAATTAT 
CATCAGTATG CCAAAGTTTT GTTTAAGAGA TACTTGGAAA TAGTAGCTGC 
TACCCCTCTA ATTATTTTGA AGAAAATAAT GGTCCTAGAG AAGTAAGATT 
ACACGACTCA CAGTTCTATA GGCAGGATAA TCAAGAGTAT TCAACTGATG 
ATGATCCTTT GCCTGGTATT GAGGGGTTTC AAATTGTTGG GGAGCCTAGA 
TCATGATTTA CATTAACAGC ATGTGGTTAC CCTACCAATG GTACTACCCT 
TTGTAATTTC CAGGTGCTAC GATGTATGAC TACGTGGTTA CTGCTGATGA 
TGTTGACACT CTTTTGGCTG TAGACTGCAC GCCTATGGAT GATAATACCT 
GTCAGGGTGA ATTGGTTACA GAGTATACTA ACAATGGGAG CAAGATAACT 
TGCGGCCAAG AAGAAGATGG TGAGGCTGAT ACAGAGGATC ATCTGGGACA 
AGAGGGCAAG GAGGGCCACC ACGGGACGCC ATCGACGTTC TCTTCAGAGA 
CACCAGCGAT GAGCTCACCG ACGAGCTCAT CTCCGACACC ATGATCGACC 
TCATGATACC CGCCGGGGAC TCCGTCCCGG TTCTCATCAC GCTCACCGTC 
AAGTTCCTCA GCGAGTGCCT TCTCGCCCTT CACCAACTGG AAGAGGAGAA 
CATGCAGCTC AAAGAGGCGA AAAACCAGCA TGGGTGAGAC CTTGCAATGG 
ACAGACATGT CATTGTCATT CACACATGTG ATAACAGAGA CGCTGTGGTT 
GGGCAACATC ATCGGTGGGA TCATGCACAA GGCGGTGCGC GGCGTCGAGG 
TGAATGGGTA CCTCATTCCC AAGGGGTGGT GTGATCGGTC CACCTCGATG 
ATATGCTCTA CGATGAGCCC TACAAGTTCA ACCCATGAAG GTCGAAGGAG 
AAGGACATGA GCAATGGCAG CTTCACTCCA TTTGGTGGTG GGCAGAGGCT 
GTGCCCAGGC CTGGATCTGG CCAGGCTGGA AGCTTCCATC TTTTTTCACC 
ACTTGGTCAC CAGCTTCAGG TTAGAACAAG GTAGCAAGGA CTGAGGCTAA 
TGGTAAGAGG AGGCAGAAGA GGAAATGAGC TAGGACTGCG GATTCCAAAA 
ATTGATTCTG TGGAGTGACC AAGTTGCAGC TAGAGAACAA GCTCCATACC 
TCCTCTTCCA TTGGGTGCCT CCTCCCCCTT GTCTCCCTCC TGTTTGCTGC 
CTCTATGGCA ATGCCTAACG GAGTAGTGGC TGGTGGACGA GAGAAAATGG 
AGTTGGGGGC AAGTGAGGAC TGTGATGGTA TGAACTATTC CTCATCATTT 
TTCAGGTGGC TAAAGTGTGT CGATCCATTC ATTAATCATA ATATGTGGAC 
TGCTCGGGAG GAGAAGACGA CTTTTACCAA CCGTCCAACA ACAAGGAATG 
TTTAACTGGA TTAACATTGC AGTTGCATTG GGTACTCACA GGACACCTTT 
CCAGTGCCTC GCTCGTTATC AGCAAAGTTT TAATCATTGT ATATTGAGTA 
AGGACTGCGC TAAAGAAGAA GATCTTCAAC TTAAAGCTGC TGTCAACACC 
TTTGGTATCA ACTGGTAACT TGTATCAGCT AGTATGGATG GTTGCACTGG 
CAATCAGTGC TCTAATAGGT GAAAAGTATT TTTGTGCCTT TACCCTTCTC 
TTGCCGTTGA CTTAATTCAT GTATCGTTTT GTTCTCTGTT ATTGGTTACT 
TTCTGTGGTC ATGTCTT

Gene models with high sequence identity

AT3G13730.1 [+] show detail - cytochrome P450, family 90, subfamily D, polypeptide 1
E-value: 5e-41; Score: 22.30

InterPro Scan Digest

Program	Description	E-value
HMMPanther	CYTOCHROME P450 90A1 (STEROID 22 ALPHA-HYDROX	0.000000000013
HMMPanther	Cytochrome P450	0.000000000013
HMMPanther	CYTOCHROME P450 90A1 (STEROID 22 ALPHA-HYDROX	6.1e-19
HMMPanther	Cytochrome P450	6.1e-19
HMMPanther	CYTOCHROME P450 90A1 (STEROID 22 ALPHA-HYDROX	0.000000000000094
HMMPanther	Cytochrome P450	0.000000000000094
FPrintScan	Cytochrome P450, E-class, group I	0.000011
FPrintScan	Cytochrome P450, E-class, group I	0.000011
HMMPfam	Cytochrome P450	0.00000000053
superfamily	Cytochrome P450	0.0000000034
PatternScan	Cytochrome P450, conserved site	0
Coil	coiled-coil	0
Gene3D	Cytochrome P450	0.0000077

See the detailed result >

AT3G13730.1

Model type: Protein coding
Short Description: cytochrome P450, family 90, subfamily D, polypeptide 1
Curator Summary: Encodes a cytochrome P-450 gene that is involved in brassinosteroid biosynthesis, most likely in the conversion step of teasterone (TE) to 3-dehydroteasterone (3DT), and/or 6-deoxoteasterone (6-deoxoTE) to 6-deoxo-3-dehydroteasterone (6-deoxo3DT); or the conversion of cathasterone (CT) to TE, and/or 6-deoxocathasterone (6-deoxoCT) to 6-deoxoTE. Recently, CYP90D1 was shown to catalyse the C-23 hydroxylation of several brassinosteroids (the enzyme has a broad specificity for 22-hydroxylated substrates). Member of the CYP90C CYP450 family. Similar to Cytochrome P450 90C1 (ROT3).
Computational Description: "cytochrome P450, family 90, subfamily D, polypeptide 1" (CYP90D1); FUNCTIONS IN: oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NADH or NADPH as one donor, and incorporation of one atom of oxygen, oxygen binding; INVOLVED IN: stamen development, petal development, leaf development, brassinosteroid biosynthetic process; LOCATED IN: endomembrane system; EXPRESSED IN: 15 plant structures; EXPRESSED DURING: 8 growth stages; CONTAINS InterPro DOMAIN/s: Cytochrome P450 (InterPro:IPR001128), Cytochrome P450, E-class, group I (InterPro:IPR002401), Cytochrome P450, conserved site (InterPro:IPR017972); BEST Arabidopsis thaliana protein match is: Cytochrome P450 superfamily protein (TAIR:AT4G36380.1); Has 27722 Blast hits to 27661 proteins in 1582 species: Archae - 80; Bacteria - 4717; Metazoa - 10119; Fungi - 4209; Plants - 7293; Viruses - 6; Other Eukaryotes - 1298 (source: NCBI BLink).
Link: InterPro Scan - TAIR