K22538

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Phenotypes

Morphological phenotypes

Generation	Anatomical entity /structure development stage	Phenotypes
T2	seed (PO:0009010)	decreased size (PATO:0000587) (Seed::Shape::small) decreased length (PATO:0000574) (Seed::Shape::short)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K22538

Determination of introduced cDNA(s)

J033070A11 - Rice Full-length cDNAs - Original: J033070A11

full reading [+] show sequence

GGTTCATTTT CCCCTCTTCC CCCGCTCCGC TCTCGTCTCC GCTCTGCTCT 
GGTCTGCTCT GCTGCGAGGG TGGGTGTTGC TTGTACCGGG CGGGGATGGA 
GGCGGCGACG ATGGCGTGGA CGGCGGCGGG GGTGGGGATG GCGCTGGTGT 
ACTGGTTCGT GTGGGTGATG GGAGCGGCGG AGGTGAAGGG GAAGCGGGCG 
GTGGATCTCA AGATGGGATC CATCACGAAC GACAAGGTGA AGGACAAGTA 
CACGCAGTAC TGGTCGTTCT TCCGCCGCCC CAAGGAGACG GCGACCACCG 
AGGCCTCCGC CGAGAAGGTG CCGGCCTTCG TCGACACCTT CTACAACCTC 
GTCACCGACA TCTACGAGTG GGGTTGGGGT CAGTCGTTCC ACTTCTCCCC 
TTCCCTCCCC GGCCGCTCCC ACCGCGAGGC CACGCGCGTC CACGAGGAGC 
GCGTCGCCGA CCTCCTCCAG GCCAAGCCGG GACACCGCCT CCTCGACGTC 
GGCTGCGGCG TCGGCGGGCC CATGCGCGCC ATCGCCGCCC ACTCCGGCTC 
CAACGTCGTC GGCATCACCA TCAACGAGTA CCAGGTGAAC CGCGCCCGCG 
CGCACAACCG CAAGGCCGGC CTCGACTCCC GCTGCGAGGT CGTCTGCGGT 
AACTTCCTCT CCATGCCGTT CTCCGACGCC TCCTTCGACG GCGCCTACTC 
CATCGAGGCC ACCTGCCACG CGCCCAGGCT GCAGGACGTC TACGGCGAGG 
TCTTCCGCGT GCTCAAGCCG GGCGGCCTCT ACGTCTCCTA CGAGTGGGTC 
ACCACCTCCC TCTACCGCGC CGACAACCCG GAGCACGTCG AGGCCATCCA 
CGGCATCGAG CGCGGCGACG CGCTCCCGGG TCTCCGCCGC CAGGACGAGA 
TCGCGTCCAT CGCCAAGGAG GTCGGATTCG AGGTGCTCAA GGAGCTCGAC 
CTCGCCCTCC CGCCCGCTCT CCCATGGTGG ACTCGCCTCA AGATGGGGCG 
CATCGCATAC TGGCGCAACT CCCTCGTCGT CCGCGTGCTC ACCATGCTCC 
GGATTGCACC CAAGGGCGTC TGCGAGGTGC ACGAGATGCT CTACGAGACC 
GCGCAGCACC TCACCCGCGG CGGCGAGACC GGCATCTTCA CGCCGATGCA 
CATGGTGCTC CTCCGCAAGC CCGTCGAGAG CAAATAGTGC AACAAAGTCA 
TCGACGACAA CAACAACACC TACCTTCATT CACTCACCAG CGGAAGAAAC 
AGAGGGAAAA CAACAAAAAA AATCTCGCAG ATCAGCAGCT GAGGTAAGAA 
GAGCAGACTA CCTACCTGGT GAGGAGGAGA GGTTGAAGAA GAGAGTGCCT 
TAAAATTAAG TTAAATTTGA TCGTTTAGAG GAGAAATAAG GATGATGTTT 
ATTGTTACTC TTTCTTGTTC GTAGCTAATT TTGCTTTCAA TTTTTTTGGT 
GGAGGATTTT GGGTTGGAAA GACAAGGTCG GGATCTCTTT TAAAGTTTGT 
TTTTCTTTTC TTTTCCCTCT TGATCGGTGG ATCGATCTAC TATCAGTATC 
AGTAATCAGC CATGCCATGT TTTGTCGAGC TCACAACATT GGAATCTCCC 
GCTAGGGAAT CAATTTTCAA CTGTAATTTG CCCCGCTTTC TGCGGTGAGA 
TTTGCAGGTG CGCAATATGT AATTCACCAT TTTCATTGCT CATCGCTCCA 
GTGCAAGAAA AAGGTTGGAA ACGACAAACA TTCTGACC

Gene models with high sequence identity

AT1G20330.1 [+] show detail - sterol methyltransferase 2
E-value: 7e-148; Score: 521.00

InterPro Scan Digest

Program	Description	E-value
HMMPanther	S-ADENOSYL-L-METHIONINE:DELTA24-STEROL-C-METH	1.2e-187
HMMPanther	METHYLTRANSFERASE	1.2e-187
superfamily	S-adenosyl-L-methionine-dependent methyltrans	2.3e-59
Gene3D	no description	1.9e-48
HMMPfam	Methyltransferase type 11	2.4e-21
HMMPfam	Sterol methyltransferase C-terminal	0.000000000000073

See the detailed result >

AT1G20330.1

Model type: Protein coding
Short Description: sterol methyltransferase 2
Curator Summary: Encodes a sterol-C24-methyltransferases involved in sterol biosynthesis. Mutants display altered sterol composition, serrated petals and sepals and altered cotyledon vascular patterning as well as ectopic endoreduplication. This suggests that suppression of endoreduplication is important for petal morphogenesis and that normal sterol composition is required for this suppression.
Computational Description: sterol methyltransferase 2 (SMT2); FUNCTIONS IN: S-adenosylmethionine-dependent methyltransferase activity; INVOLVED IN: xylem and phloem pattern formation, negative regulation of DNA endoreduplication, multidimensional cell growth, sterol biosynthetic process, pattern specification process; LOCATED IN: endoplasmic reticulum; EXPRESSED IN: 30 plant structures; EXPRESSED DURING: 14 growth stages; CONTAINS InterPro DOMAIN/s: Sterol methyltransferase C-terminal (InterPro:IPR013705), Methyltransferase type 11 (InterPro:IPR013216); BEST Arabidopsis thaliana protein match is: sterol methyltransferase 3 (TAIR:AT1G76090.1); Has 13231 Blast hits to 13223 proteins in 2296 species: Archae - 440; Bacteria - 9200; Metazoa - 162; Fungi - 455; Plants - 664; Viruses - 0; Other Eukaryotes - 2310 (source: NCBI BLink).
Link: InterPro Scan - TAIR