K23244

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K23244

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T2 seed (PO:0009010)
  • decreased width (PATO:0000599)
    (Seed::Shape::narrow)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K23244

Determination of introduced cDNA(s)

  • J023145I07 - Rice Full-length cDNAs - Original: J023145I07

    full reading [+] show sequence

    Nucleotide sequence: J023145I07 - full reading 
    GTCGCTGCTC CCTATTCCCC ATTCCCCCCA CGCGACAGTG GAGACGACAA 
    GTGAAGCGAT CGCCACAAGC CGCCATCCGC AGCGCCGACT CCGACGCCGG 
    ACGCCGGGCG CCGCGTCGGC GCCGAGACGC TCACGCCGGC CGGCCATCAC 
    GGTATAGCCA CCACCGTGTC CGGCATCCGG CCAGGCGGCG CAGGCGGCAG 
    GGGAAGATTT CAGAAAGCCT GAGTTTGGTT GGTATTGTTG CGCGAAGATA 
    AACAGGAAAG AAAGTGAGAT CAGGGAACAA GTGTCTAAGG AGAAAGTGTG 
    TGAAGACGAG GCGAGCTCCC TTTTGGGAGA AGATTCCTTT TTAGGCATAT 
    GCTTCCCTAC AAAACTTCCC CAAAGTGAAC CTGGAGGAGA TTTTTTTTTT 
    GGCGAGAGAG AGAGAGTGGG AGGGGCACCT ACCCCTTCAA GCCCACGACC 
    ACGGCCGCCG CCTCTTCTTG TGCACAAGGG GACGTTGTGT GCTTCGTGCA 
    CACGCATGGC CGACACGAGA GGCTCCACTT CCTCTGGCGG CGGCGATGAC 
    AGGGGCAGAG AAGGCCACGA TGACTTCACA GGGGGCGGCC AATACAACAG 
    GTACCATAGG ATCCTTGAGG CCGTGCCGTC ACCCCTTGTT AGGCGTGAGA 
    ATGGTGTGCA TCATCAGTAC CCCACTGGCC TAATCCACCA CCCGTCGTCG 
    ACAATGCCGG TGGCGCCATG CTCCTACGTG CCCCGGTACA CCATGGTGCC 
    AACATCCGCC ATGCTCCCGC TGCAGCACCA CCACCGCCAA TTGCAGATAT 
    CTCAAGAAGA CTTCCAAGAC CGAGTTCCCT CCAATAATGT TGCAGCTCCG 
    CATCTTCCTA GCAATTTTCA GGATTTGAGG CCAATGTGCA ATGGGCCGCC 
    ATTCATGTCT TATGGCCAGA CTGCTTCCAA TAGAAATGTT CTTTATCAGA 
    ATCTGACGCC ATACTCCTTC AATGCATGGG CTAGCAATAA CATGCCGAGG 
    AACCCTGTCT ACACTAGTTA CCACCCTACT GCAATTGAGG ATCCCCATGC 
    TACTCCATTC CACATCAACA ATCATGACAC AGACCAAGGT TTCTTCACCG 
    TTTCTACTAG CTTCAGGGTG GATCAATCAT TTGTACATGC TCCTTCACCC 
    TTTCCACCAG TGTCTTCATC ATCCCGCAGT TTCTCTTCAG CTCAAATTTC 
    AAATGGACCG ATAGACGCTA AGAAAGCCAA GAAATCAGAT ATAAAAGATC 
    AACCAATTGT TTTAAGAAGA AGTGATACGG AGAGTGAAAA AAATGATGAA 
    CTAGATCAAA CTCCGGCTAG TGAGCCTTCA AGCATGAGTC ATAACAGTGC 
    AAATTCGACT ATTAGATTCA ACTGTAGAGA ATACCGTGTT ATCTTGCGCA 
    AGGAGTTGAC AAATAGTGAT GTTGGTAATA TTGGAAGAAT TGTGATGCCA 
    AAGAGGGATG CAGAGGCTCA TCTTCCAGCA TTGCATCAAA GGGAAGGTGT 
    GATGCTGAAA ATGGATGACT TCAAGCTTGA AACTACTTGG AATTTTAAGT 
    ACAGGTTCTG GCCCAACAAC AAGAGCAGAA TGTATGTCTT GGAAAGCACG 
    GGTGGCTTTG TGAAGCAGCA TGGTCTCCAG ACAGGGGACA TATTCATCAT 
    CTACAAAAGC TCGGAGTCTG AGAAATTAGT TGTTCGTGGG GAGAAGGCCA 
    TTAAGCCGAA TGTCATCATG CCTATTGTGG ACTGCAGCTG CAAAAATGAT 
    CTCAACAACA GCGAAGAATG CGGGTTCGCT ATCAGCCTGC TGACTAAGAA 
    AACCTGATGT GGAATGGCAG CTTCATGTCT TGAGACCATC ATCTGAAAGC 
    CTCATTACAA GGCAATGCAT TTTTGTTATT GGAGAATGTG TTGTCCCACA 
    CTTTTAACTT CATAGATAAA AGTACAAGAC TATGGATTAT TATTTAGTAA 
    CTCTTTTTTT TTCCAAATAA TAGTCCACAT CAGAGATTGC AACAGCC

    Gene models with high sequence identity

    1. AT3G24650.1 [+] show detail - AP2/B3-like transcriptional factor family protein
      E-value: 5e-31; Score: 133.00

     

    InterPro Scan Digest

    Program Description E-value
    ProfileScan Transcriptional factor B3 9.079
    HMMPfam Transcriptional factor B3 8.8e-20
    Gene3D no description 3.3e-31

    See the detailed result >

    AT3G24650.1

    Model type
    Protein coding
    Short Description
    AP2/B3-like transcriptional factor family protein
    Curator Summary
    Homologous to the maize transcription factor Viviparous-1. Full length ABI3 protein binds to the highly conserved RY motif [DNA motif CATGCA(TG)], present in many seed-specific promoters, and the B3 domains of this transcription factor is necessary for the specific interaction with the RY element. Transcriptional activity of ABI3 requires the B3 DNA-binding domain and an activation domain. In addition to the known N-terminal-located activation domain, a second transcription activation domain was found in the B1 region of ABI3. ABI3 is essential for seed maturation. Regulator of the transition between embryo maturation and early seedling development. Putative seed-specific transcriptional activator. Mutants exhibit decreased responsiveness to ABA suggesting that ABI3 protein participates in the ABA perception/transduction cascade. Based on double mutant analyses, ABI3 interacts genetically with both FUS3 and LEC1 and is involved in controlling accumulation of chlorophyll and anthocyanins, sensitivity to abscisic acid, and expression of the members of the 12S storage protein gene family. In addition, both FUS3 and LEC1 regulate positively the abundance of the ABI3 protein in the seed. Alternative splicing of ABI3 is developmentally regulated by SUA (AT3G54230).
    Computational Description
    ABA INSENSITIVE 3 (ABI3); CONTAINS InterPro DOMAIN/s: Transcriptional factor B3 (InterPro:IPR003340); BEST Arabidopsis thaliana protein match is: AP2/B3-like transcriptional factor family protein (TAIR:AT3G26790.1); Has 4494 Blast hits to 2758 proteins in 300 species: Archae - 0; Bacteria - 132; Metazoa - 1104; Fungi - 405; Plants - 1155; Viruses - 101; Other Eukaryotes - 1597 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR