K23812

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K23812

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T2 seed (PO:0009010)
  • decreased size (PATO:0000587)
    (Seed::Shape::small)
  • decreased length (PATO:0000574)
    (Seed::Shape::short)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K23812

Determination of introduced cDNA(s)

  • J013025D11 - Rice Full-length cDNAs - Original: J013025D11

    full reading [+] show sequence

    Nucleotide sequence: J013025D11 - full reading 
    GGGCGGTCTC CTCTCCATTT TTTTCCTCCT ATCGATTTTG CAATTTAGCC 
    CCTAGGGAGG CAGGGAATCC GGCTCCGGTC CCTGTGCCGC TCCCCCCGGC 
    TCTCCTTGCC TCCCTTGCTC CTCCGATCGA GGCTGAAGCG GCTTCAGGAT 
    ATACATTTTT GTGGGAATGG GGAATTCTTG CCAAAATGGA ACCTATGGGA 
    ACAATTACCA GAACAGCAAC CGGTTTCAGA ATGACCGTTT TGCTTCTCGG 
    TACGTTGATG GGAATGATAC TGAGGATTGC TACTCGGGCT CGTCAAGGGC 
    CAGCTTAGCG GGTGCTCTGC GGCAAGGGCT GAACCTAAAG TCCCCTGTCC 
    TTGGATACAA GACTCCAAAT GTAAGGGAGC TCTATACTCT TGGCCGGGAG 
    CTTGGACAGG GACAGTTCGG GAAAACATAC CTCTGCACTG AGATTAGCAC 
    AGGGTGTCAA TATGCATGCA AGACTATCTT AAAGAGTAAT CTCCGATGTG 
    TGTCAGATAT CGAGGACGTG CGCCGTGAAA TCCAGATAAT GCACCATCTT 
    TCGGGCCAGA AGAATATAGT GACAATCAAG GACACATATG AGGATGAGCA 
    GGCCGTGCAC ATCGTCATGG AGCTCTGTGC AGGTGGTGAG CTCTTCAGCA 
    AGATTCAGAA GCGAGGTCAT TACAGTGAAC GGAAGGCTGC AGAGCTTATA 
    AAAATTATAG TTGGCATCAT AGAAACATGC CATTCACATG GAGTGATGCA 
    CCGGGATCTC AAGCCAGAAA ATTTCCTCTT ACTGGACGCA GACGATGAAT 
    TCTCGGTTAA AGCAATTGAC TTTGGTCTAT CTGTGTTCTT CAGACCAGGT 
    CAGGTTTTCA GAGAGGTAGT GGGAAGTCCA TATTATATTG CTCCAGAGGT 
    ATTGGAGAAG CGTTATGGAC CAGAGGCTGA TATATGGACT GCTGGAGTGA 
    TTCTCTATGT ATTGCTGACT GGTGTTCCTC CATTTTGGGC AGATACACAA 
    AGCGGGATAT ATGAAAAAGT ACTGGATGGA CGTATTGATT TTAAATCAAA 
    CCGGTGGCCC AGGATATCTG ACAGTGCAAA GGATCTTATA AAAAAGATGC 
    TCTGCCCTTA TCCATCAGAG CGTTTGAAAG CCCATGAAGT GCTAAAGCAT 
    CCATGGATAT GTGATAATGG AGTGGCTACT AATCGAGCTC TGGATCCAAG 
    TGTACTTCCT CGTCTCAAGC AATTTTCTGC AATGAATAGG TTAAAGAAAT 
    TGTCTCTCCA GATTATTGCT GAGCGTCTTT CAGAAGAGGA GATTGTTGGG 
    TTAAGAGAAA TGTTCAAGGC TATGGACACC AAAAACAGAA GTGTGGTTAC 
    ATTTGGTGAG CTTAAGGGAC TGAAAAGATA CAGCTCAGTG TTCAAGGATA 
    CTGAAATTAA CGACTTAATG GAAGCAGCTG ATGACACCAC CTCTACCATC 
    AACTGGGAAG AGTTTATTGC TGCAGCAGTG TCTCTTAATA AAATAGAACG 
    TGAGAAACAC TTGATGGCAG CCTTTACATA CTTTGACAAA GATGGAAGTG 
    GTTTTATCAC AGTTGACAAG CTTCAAAAGG CTTGCATGGA ACGTAACATG 
    GAAGATACTT TCCTTGAAGA GATGATTCTG GAGGTTGATC AAAACAATGA 
    TGGTCAAATC GATTATGCTG AATTTGTCAC AATGATGCAA AGCAACAACT 
    TTGGACTTGG GTGGCAAACG GTGGAAAGCA GCCTGAATGT AGCCCTGAGG 
    GAGGCACCCC AAGTATACTG AACTCCTGTC GCCTGGCACC CCCAAGAATT 
    CAGTTTGTTC TCCTGAGCCT TTCATCTGTT TATCACAACA CATAGTTGTG 
    GATTTGAGAG AAGAGAGCCA AGACTAGATG GTTTATCAGT AATCACCCTA 
    TAGCAGTGGT GGAAGAAGAC TCTCTTAGTA CCTAATAGCA TATAAATGTG 
    TGGTCTAAAG ACATCGTATA TGTATGAACC TCATAAGTTC GTTCGTTCGT 
    TCGTTTGGTT GGTTGGTTTG CTACCCTGCT ATCTAATAAT AATATCAATA 
    ATACCCGAAG AACCCCTCTT GGTTCTGC

    Gene models with high sequence identity

    1. AT2G17290.1 [+] show detail - Calcium-dependent protein kinase family protein
      E-value: 0; Score: 694.00

     

    InterPro Scan Digest

    Program Description E-value
    HMMSmart Serine/threonine-protein kinase domain 8.6e-93
    HMMSmart Tyrosine-protein kinase, catalytic domain 5.6e-11
    HMMSmart Calcium-binding EF-hand 0.82
    HMMSmart Calcium-binding EF-hand 1.7e-05
    ProfileScan Protein kinase, catalytic domain 45.418
    ProfileScan EF-HAND 2 7.456
    ProfileScan EF-HAND 2 13.174
    ProfileScan EF-HAND 2 8.432
    PatternScan EF-Hand 1, calcium-binding site 0
    PatternScan Protein kinase, ATP binding site 0
    PatternScan Serine/threonine-protein kinase, active site 0
    HMMPfam Serine/threonine-protein kinase-like domain 3.6e-67
    HMMPfam EF-hand 1.8e-07
    Gene3D no description 9.9e-24
    Gene3D no description 2.3e-56
    Gene3D EF-hand-like domain 8.5e-30
    HMMPanther Calcium-dependent protein kinase 5e-244
    HMMPanther Calcium/calmodulin-dependent protein kinase-like 5e-244
    superfamily Protein kinase-like domain 7.4e-85
    superfamily EF-hand 9.8e-54

    See the detailed result >

    AT2G17290.1

    Model type
    Protein coding
    Short Description
    Calcium-dependent protein kinase family protein
    Curator Summary
    Encodes calcium dependent protein kinase 6 (CPK6), a member of the Arabidopsis CDPK gene family. CDPKs contain an intrinsic Ca2+-activation domain with four EF hand Ca2+-binding sites. CDPKs protein kinases have been proposed to function in multiple plant signal transduction pathways downstream of [Ca2+]cyt elevations, thus transducing various physiological responses. CPK6 is expressed in both guard cells and mesophyll cells. Functions in guard cell ion channel regulation. ABA and Ca(2+) activation of slow-type anion channels and, interestingly, ABA activation of plasma membrane Ca(2+)-permeable channels were impaired in independent alleles of single and double cpk3cpk6 mutant guard cells. Furthermore, ABA- and Ca(2+)-induced stomatal closing were partially impaired in these cpk3cpk6 mutant alleles. CPK3 is also a member of the Arabidopsis CDPK family.
    Computational Description
    calcium dependent protein kinase 6 (CPK6); FUNCTIONS IN: in 6 functions; INVOLVED IN: regulation of anion channel activity, protein amino acid phosphorylation, N-terminal protein myristoylation, abscisic acid mediated signaling pathway, regulation of stomatal movement; LOCATED IN: cytosol, plasma membrane, membrane; EXPRESSED IN: 26 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Protein kinase, ATP binding site (InterPro:IPR017441), EF-Hand 1, calcium-binding site (InterPro:IPR018247), Serine/threonine-protein kinase domain (InterPro:IPR002290), EF-hand-like domain (InterPro:IPR011992), Calcium-binding EF-hand (InterPro:IPR002048), EF-hand (InterPro:IPR018248), Serine/threonine-protein kinase-like domain (InterPro:IPR017442), Protein kinase-like domain (InterPro:IPR011009), Serine/threonine-protein kinase, active site (InterPro:IPR008271), Protein kinase, catalytic domain (InterPro:IPR000719), EF-HAND 2 (InterPro:IPR018249), Calcium-dependent protein kinase (InterPro:IPR020642), Calcium/calmodulin-dependent protein kinase-like (InterPro:IPR020636); BEST Arabidopsis thaliana protein match is: calmodulin-domain protein kinase 5 (TAIR:AT4G35310.1); Has 133264 Blast hits to 125497 proteins in 3863 species: Archae - 149; Bacteria - 13464; Metazoa - 49073; Fungi - 17503; Plants - 30048; Viruses - 473; Other Eukaryotes - 22554 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR