K24016

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K24016

Phenotypes

Morphological phenotypes

Specific phenotypess were NOT observed.

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K24016

Determination of introduced cDNA(s)

J013052K11 - Rice Full-length cDNAs - Original: J013052K11

full reading [+] show sequence

GACGTCCAAT TTCTTTCTCC AAGAAAGAAC GCCGAACAAG CGCCGACGAA 
AAACGGTTCC TCGTTATTTC TCTCCAACGC GCGCGCACGG GCTATTTCTA 
CCCCCGCCGC CGAGTAGTTA GGCGTCCAAA GTTTCGGGAG TTCATCGTGA 
ACGGTGATCG ATCCAAAGAA CAACAACTCG GAGCAAAGCT AGCTAACGAA 
GTTAGTTCCA AACAAAGCTA GCTTATCGCG ATTAGCTAGC TAGAAGAGAG 
TTAGCTAGGC GCCACGCAGC TAGGCGTGCC ACTGCCACGA CGGCCCGTGC 
CCGGTAGCTA CGGCGTGCCG TTCGTCTCGG CGGTGCGCGA CCGCCTCGAT 
TTCTACTACT TGCAGGGGCA GGACAAGTAC TTCGAGTCGC GCGCCGAGAG 
GTACGGCTCC ACCGTCGTCC GCATCAACGT CCCGCCTGGC CCATTCATGG 
CGCGCGACCC CCGCGTGGTG GCGCTCCTCG ACGCCAAGAG CTTCCCCGTC 
CTCTTCGACG TCGCCAAGGT CGAGAAGCGG GACGTGTTCA CCGGCACGTT 
CATGCCGTCC ACCTCCCTCA CCGGCGGCTA CCGCGTCTGC GCCTACCTCG 
ACCCGTCCGA GCCCAACCAC GCCAAGATCA AGCAGCTGCT CCTCTCCCTC 
CTGGTCTCTC GCAAGGACGC CTTCGTCCCG GTCTTCCGCT CCAACTTCGG 
CGCGCTCCTC GACACCGTCG AGTCGCAGCT CGCGAGCGGC GGCGGCAAGT 
CCGACTTCAC CGCCCTCAAC GATGCCACCT CCTTCGAGTT CATCGGCGAG 
GCGTACTTCG GCGTGCGTCC CTCCGCGTCG AGCTCCCTCG GCACCGGCGG 
GCCGACCAAG GCCGCCCTGT GGCTCCTATG GCAGCTCGCC CCGCTCACCA 
CGCTCGGCCT GCCCATGATC ATCGAGGATC CGCTCCTCCA CACGCTGCCG 
CTGCCACCCT TCCTCATCAG CTCCGACTAC AAGGCGCTGT ACGCGTACTT 
CGCCGCCGCG GCGTCGCAGG CGCTCGACGC CGCCGAGGGC CTTGGCCTGT 
CGCGGGAGGA GGCCTGCCAC AACCTGCTGT TCGCGACGGT GTTCAACAGC 
TACGGCGGCT TCAAGCTGCT GCTCCCGCAG ATCCTGGCGC GCGTCGCGCA 
GGCCGGCGAG AAGCTCCACG AGAGGCTCGC CGCGGAGATA CGGAGCGCGG 
TGGCCGACGC CGGCGGCAAC GTGACGCTGG CCGCTCTGGA GAAGATGGAG 
CTGACCAGGT CGGTGGTGTG GGAGGCGCTG CGGCTGGACC CGCCGGTCAG 
GTTCCAGTAC GGGCGCGCCA AGGCCGACCT GGAGATCGAG AGCCACGACG 
CGTCGTTCGC GATCAAGAAG GGGGAGATGC TGTTCGGCTA CCAGCCGTGC 
GCCACCAGGG ACCCGCGGGT GTTCGGCGCC ACGGCGAGGG AGTTCGTCGG 
CGACCGGTTC GTCGGCGAGG AGGGGAGGAA GCTGCTGCAA TACGTGTACT 
GGTCGAATGG GCGAGAGACG GAGAACCCTA GCGTTGACAA CAAGCAGTGC 
CCCGGCAAGA ACCTGGTGGT GCTCGTCGGA AGGCTGTTGC TCGTCGAGCT 
CTTCCTCCGG TACGACACCT TCACCGCCGA GGCCGGCAAA AAGGTGGTCA 
TCACCGGGGT CACCAAAGCT TCAACCTCCG CCGTCAATCG TACTGCTTAA 
GCCGGCCATC ACTTCAGGCG TGGGTCGTCG ATCATCCACT CAACCAGCTC 
CGTCCTACGC ATATGCAATA AGAAATACTC GTACCTGAAT TCTTGCATGG 
CTAAACACTA CTAATAAGTG TATGTATTCT GTTTATTTGT TCGATTCCAT 
ATTTGTAATT TTGTTGTTAT TGTTTGCTGT TGAATTACGA TTGTGTATTT 
TACAGGGCTG ACAGTGTCTT TAAACACCTC TAATATGTAT CGGATCAATC 
TGCCCTAGTC ATGTCTAATT TGTG

Gene models with high sequence identity

AT5G42650.1 [+] show detail - allene oxide synthase
E-value: 1e-122; Score: 437.00

InterPro Scan Digest

Program	Description	E-value
HMMPfam	Cytochrome P450	1e-11
superfamily	Cytochrome P450	3.4e-49
Gene3D	Cytochrome P450	3e-33
HMMPanther	Cytochrome P450	9.8e-09
Coil	coiled-coil	0

See the detailed result >

AT5G42650.1

Model type: Protein coding
Short Description: allene oxide synthase
Curator Summary: Encodes a member of the cytochrome p450 CYP74 gene family that functions as an allene oxide synthase. This enzyme catalyzes dehydration of the hydroperoxide to an unstable allene oxide in the JA biosynthetic pathway. It shows a dual catalytic activity, the major one being a 13-AOS but also expressing a 9-AOS activity.
Computational Description: allene oxide synthase (AOS); FUNCTIONS IN: hydro-lyase activity, allene oxide synthase activity, oxygen binding; INVOLVED IN: in 7 processes; LOCATED IN: in 7 components; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Cytochrome P450 (InterPro:IPR001128); BEST Arabidopsis thaliana protein match is: hydroperoxide lyase 1 (TAIR:AT4G15440.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink).
Link: InterPro Scan - TAIR