K24331

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K24331

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T2 seed (PO:0009010)
  • increased size (PATO:0000586)
    (Seed::Shape::large)
  • decreased width (PATO:0000599)
    (Seed::Shape::narrow)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K24331

Determination of introduced cDNA(s)

  • J013000O12 - Rice Full-length cDNAs - Original: J013000O12

    full reading [+] show sequence

    Nucleotide sequence: J013000O12 - full reading 
    GCTTCTCTAA CCCCCTGCTG TTGCTAGCAG AAGTCCAGGT GGACAAGAAC 
    GCGAGGAGGG GAAGAATCAT CCCCGAGATC TCGCTGCCGC TGCTCATGGC 
    GTTCGCGGCG TCCCACACCG CATCGCCGTC CTCCTGCGGC GGTGTGGCGC 
    AGAGGAGGAG CAATGGGATG TCGCCGGTGG TGGCCATGGC CTCCACCATC 
    AACAGGGTCA AGACTGCTAA GAAGCCCTAC ACTCCTCCAC GTGAAGTTCA 
    TCTCCAAGTC AAGCATTCAC TGCCACCCCA AAAGCGGGAG ATTTTCGATT 
    CGCTTCAACC TTGGGCCAAA GAGAACCTTT TGAACCTCCT GAAGCCAGTT 
    GAGAAGTCAT GGCAGCCACA GGACTTCCTG CCAGACCCTT CTTCCGATGG 
    GTTTTATGAT GAAGTAAAAG AGCTGCGGGA GAGGGCTAAG GAGATCCCTG 
    ATGACTACTT TGTTTGCTTA GTTGGAGACA TGGTTACTGA GGAAGCTCTT 
    CCTACCTATC AAACAATGCT TAACACCCTT GATGGTGTCC GAGATGAAAC 
    TGGCGCAAGC CCAACCACCT GGGCTGTTTG GACAAGAGCA TGGACTGCTG 
    AAGAGAACAG GCATGGTGAT CTTCTTAATA AGTACATGTA CCTTACTGGA 
    CGTGTTGACA TGAAACAAAT TGAGAAGACA ATACAATACC TGATTGGGTC 
    TGGGATGGAT CCAGGAACTG AGAATAATCC CTACTTGGGT TTCCTTTACA 
    CATCATTTCA AGAAAGAGCT ACATTTATAT CCCATGGCAA TACTGCAAGG 
    CACGCCAAGG AGTACGGGGA CCTTAAGCTG GCTCAGATAT GTGGGACAAT 
    AGCAGCTGAT GAGAAGCGCC ATGAGACAGC TTACACCAAG ATAGTGGAGA 
    AGCTCTTCGA GATTGATCCT GACTACACAG TTCTTGCATT TGCTGACATG 
    ATGAGGAAGA AGATCTCAAT GCCTGCTCAT CTGATGTATG ACGGCAAGGA 
    CGACAACTTG TTCGAGCATT TCAGCGCTGT GGCACAGCGG CTGGGTGTCT 
    ACACTGCAAG AGACTATGCC GACATCCTGG AGTTCTTGGT CCAGAGGTGG 
    AAAGTCGCAG ATCTCACCGG GCTGTCCGGA GAAGGGAGAA GGGCTCAGGA 
    TTTCGTCTGC ACGTTGGCGC CGAGAATCAG GCGGCTGGAT GAAAGAGCCC 
    AAGCAAGAGC TAAGCAAGCG CCTGTTATTC CTTTCAGCTG GGTTTATGAC 
    CGCAAAGTGC AGCTTTGATC ATGATCAGTG AGCAACCGAG ACTGCATCCT 
    ATATATTGGT TCTAAGATAT GAGGTTCTCT ATATGGAAAA AAATCTAGGT 
    TATCTAGCTG GGGTAGAATG CAATTACCTG TCCATCTGCC CTCACAAAAT 
    CTGTTGTCTT TTAAGTTGAT AACAGTGTCG ATGAGTCTGG TGAGAAGCTG 
    ATGTGAGATG CAACAGAGTG AACTTATGTT GTATTGATGT GAGATGAGTA 
    TGGTGACATT TATGTGTACT TGCAGGTTTT ACATGACTTG TATAAGTTTT 
    AAAGTAATAA TGGAGATTTG TATACAGACA AGCGTGTGCA CTGTCGCATG 
    ATTAAGTAGT CTAAAATCCA GACTATCTTT TGTGCTCTGA ATTTAGCAGT 
    AATAAGATTG ATCGGCTTGT TCGTTTTG

    Gene models with high sequence identity

    1. AT2G43710.1 [+] show detail - Plant stearoyl-acyl-carrier-protein desaturase family protein
      E-value: 5e-171; Score: 598.00

     

    InterPro Scan Digest

    Program Description E-value
    superfamily Ferritin/ribonucleotide reductase-like 1.3e-165
    Gene3D Ribonucleotide reductase-related 3.6e-156
    HMMPIR Fatty acid desaturase, type 2 8e-269
    PatternScan Stearoyl-ACP desaturase, conserved site 0
    HMMPfam Fatty acid desaturase, type 2 6.2e-188

    See the detailed result >

    AT2G43710.1

    Model type
    Protein coding
    Short Description
    Plant stearoyl-acyl-carrier-protein desaturase family protein
    Curator Summary
    Encodes a stearoyl-ACP desaturase, involved in fatty acid desaturation. The ssi2 mutants have increased 18:0 and reduced 18:1 fatty acids. Exogenous application of glycerol to wild type plants mimics the ssi2 mutant phenotype. The altered 18:1 fatty acid content in the ssi2 mutants has an impact on SA- and JA-mediated defense signaling. ssi2 mutants resulted in hyper-resistance to green peach aphid and antibiosis activity in petiole exudates.
    Computational Description
    SSI2; FUNCTIONS IN: acyl-[acyl-carrier-protein] desaturase activity, stearoyl-CoA 9-desaturase activity; INVOLVED IN: in 10 processes; LOCATED IN: chloroplast, chloroplast stroma, plastid; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Ribonucleotide reductase-related (InterPro:IPR012348), Ferritin/ribonucleotide reductase-like (InterPro:IPR009078), Fatty acid desaturase, type 2 (InterPro:IPR005067), Stearoyl-ACP desaturase, conserved site (InterPro:IPR005803); BEST Arabidopsis thaliana protein match is: Plant stearoyl-acyl-carrier-protein desaturase family protein (TAIR:AT3G02630.1); Has 948 Blast hits to 940 proteins in 221 species: Archae - 0; Bacteria - 436; Metazoa - 2; Fungi - 0; Plants - 452; Viruses - 0; Other Eukaryotes - 58 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR