K25302

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K25302

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T2 seed (PO:0009010)
  • increased width (PATO:0000600)
    (Seed::Shape::wide)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K25302

Determination of introduced cDNA(s)

  • J033067G13 - Rice Full-length cDNAs - Original: J033067G13

    full reading [+] show sequence

    Nucleotide sequence: J033067G13 - full reading 
    GATCCGTGAC GCGAGGCAGT GCGGTGCGGA TTCCATTCTC CTCTTGGCAT 
    CACGAGGCGG CGCCGCTTGG TCTAGCTAGT AGCCACAGGG AGAGGTGGTA 
    GCCGCAGCCG CCGCCGACGA GACCTCGCCG CCGGGGGGAG GGCACCATGT 
    GTGGCATCCT CGCCGTGCTC GGCGTCGCAG ACGTCTCCCT CGCCAAGCGC 
    TCCCGCATCA TCGAGCTATC CCGCCGGTTA CGTCATAGAG GCCCTGATTG 
    GAGTGGTATA CACTGCTATC AGGATTGCTA TCTTGCACAC CAGCGGTTGG 
    CTATTGTTGA TCCCACATCC GGAGACCAGC CGTTGTACAA TGAGGACAAA 
    TCTGTTGTTG TGACGGTGAA TGGAGAGATC TATAACCATG AAGAATTGAA 
    AGCTAACCTG AAATCTCATA AATTCCAAAC TGCTAGCGAT TGTGAAGTTA 
    TTGCTCATCT GTATGAGGAA TATGGGGAGG AATTTGTGGA TATGTTGGAT 
    GGGATGTTCG CTTTTGTTCT TCTTGACACA CGTGATAAAA GCTTCATTGC 
    AGCCCGTGAT GCTATTGGCA TTTGTCCTTT ATACATGGGC TGGGGTCTTG 
    ATGGTTCGGT TTGGTTTTCG TCAGAGATGA AGGCATTAAG TGATGATTGC 
    GAGCGATTCA TATCCTTCCC CCTAGCTAGC ACGTACTCCA GTAGACTGCG 
    ATGGGCGCGC CGCCGACCAG GGAATCCGAA GCCCGCGACG AGGAGGCGGA 
    GGTGTCCCGA TCGGGTGAGC AAGGCGGCGG TGAGGCCTCT ACGACGCAGC 
    AGCAGCAGCA GCCGGTGCCT CTGCGCCACC AGCTGCTCGG CGCCTGCCGC 
    GCCGACGAAC GCCTCAGGCC CCTCCTCACT CTCAACCTCT CATGCGGCGC 
    CGCCGAGGAC CGCTTCATCT CCCACCTCTC GCAGCACTTC GAGGCGTCCG 
    AGGTCGGCTT GCTCTACAGG TGTCTCTGCG TTCCCCTCGT CGCTCTTCGC 
    GTCGGCAAGG TCGACCGCCA TGGTCCTCTC CTCTGCCCAA CGCCCATCAG 
    GTAATTTGGG CGTGTGTCAT AATCTCTGAT ATATGAGGAA AGAGAAGAGG 
    AAAGCTGAGC CTTGGTCTTC TTCCTTCATC GAGCATGTGC ATTATCTTTG 
    CTGGTGATGA TGGTCATTCG GAGCAACTAG CTCTCCTGAA CAATGACCAT 
    GAAGTTTCAG AAGTTTGTGT TGAAGAAATT TCAGCTGATA ATACAGGACG 
    ATCTTTCCTC ATCAGGATTT CAGAATCTAA AGTTTTTTAC TATTGGTGTG 
    CTGAGAAGTC AAAGAAACAC GGGATGGACC TTCTTGCAAA GATGAAAAAT 
    CTACTGCAGG GAAGGCCAAC ACTCTCTGAT CTCACAGGCA TCTCAGATTC 
    ACGACTGGAT GCTTTGCCAC TCATCTACAC GCTTATCTTG TTGCATCAAG 
    TATTGGAGAT GTTAAATCGC TGGGATCACT TAATGACTTC CTGGGCGCTT 
    CAAGCCCACA AGATCAATAT TTACAGCCCT CATCAGTTGT TTCCAAGTCT 
    TCACGCTTCT GCACCTCTGC AGCTAATGCA GCAAAAGCAA GTTCTGTCTA 
    CCAAACTAGT CTGAGTCCCA GATGTGGTGC TTTTAAAGAT GGAGTGCCAA 
    GGATGTCGTG TGCAAAGATT GCTGGAAGAG ACAAGCTGAA GCGACGTGGG 
    GACTGGCTGA GCTCATCAAC TGGTCCTGAT GACGCAAATC TTTTGACACC 
    GAAGATTGTT AGCTCTGATT CTGCCAGTGA GAAGTGTGGT GGGGACTGTT 
    CTGAAAACAG TGCTAATTCA CCTCCTCTAG ACTTGCCTCT TTCATTTCCC 
    TTGTTGCCAT CTCTGTTTCC TCTTGCGACT CAATATCCAC TTCCTAAGGA 
    TTCTACAGAG CAACCATTTA AGCCTTACTA TTGCTGGTGC CCACCATGCC 
    CATCTTCTCT GCAGTACAGT GTAACCCCTC TGCATATGCC AGTTACATCT 
    GTAGAACCAC TGCCCTTGCC GCCTTTGAGT TCTCTGCTAT CAAATGATCA 
    GCCGCCGACT TCAACAGTTT CTGCAAAGAT GGATACAACT GATCTCCCGT 
    CACTTAATCT TCCATCGATA CTGCGTGATC CTTTACTTCA CCTGCCGCTT 
    CCTACTTCAC CACTTGTATC CTTGCATGGT TCACAAGTTC CAACTTTCAC 
    ACCATTGATG TCTGACCCCA TTGTGCATGT CCCGGTCATT GATGTGTGTT 
    CCTCTGGCCA GGCTTACCTG GTGAGTTGCG GGCCTTCTAT GTCTGCGACT 
    GTTACCCTGC TCCCTAGCTT GAAGCCCCTG ATTCCAGAAA CAGAATCTTT 
    GGTTGAGAGA AGCGCTAGGG AAACACTAAT GAGGCTTATA GCCTCAACAC 
    CATCTGCAAG TAATCCCCAG CTGGTGAACA TTCTCCCTGC AGTCCTTACT 
    GACGTGCCTG AAATGAATGT GAGGAAACAT CTTGGTGTCC ATCCAGGCGA 
    CAGGCTAAGC TCATCTTGTA GCGTCGATGT GATTGGACCT GGGTTTGCCG 
    TCACAGAGGA TGATGCATCT GTTGGAGATG GGGCTCACGC AACTTTTGCG 
    GAATATGATG ATATCGGCGA TCAGCAGCAT TTTCAGAGCA TGTAGGTGGT 
    AGGTTGTCGA CAAGGTACTC GTTGGTTGTC CAGAATATTT TGAAAGGCAG 
    TTGTATCTAT ATGGTAATAG CTTAGCGTTG GGTTTACTTT TGTGACGCTC 
    TGCGTACTGC CTATTTGCTG TGATGATTTA GCTGCTCAGT CAAAAACCTC 
    ATTAGGTTGG TCAATGTAAT TAGCTGTTTG TAGTGTATTC ATTCATAAAA 
    CTAAAAGCAA ACTACCCAGT TAATTTGCG

    Gene models with high sequence identity

    1. AT5G10240.1 [+] show detail - asparagine synthetase 3
      E-value: 1e-80; Score: 298.00

     

    InterPro Scan Digest

    Program Description E-value
    superfamily N-terminal nucleophile aminohydrolases (Ntn h 6.3e-74
    Gene3D no description 4.6e-57
    HMMPanther ASPARAGINE SYNTHETASE 1.7e-51
    ProfileScan Glutamine amidotransferase, type II 34.716

    See the detailed result >

    AT5G10240.1

    Model type
    Protein coding
    Short Description
    asparagine synthetase 3
    Curator Summary
    Encodes asparagine synthetase (ASN3).
    Computational Description
    asparagine synthetase 3 (ASN3); FUNCTIONS IN: asparagine synthase (glutamine-hydrolyzing) activity; INVOLVED IN: asparagine biosynthetic process; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Rossmann-like alpha/beta/alpha sandwich fold (InterPro:IPR014729), Asparagine synthase (InterPro:IPR001962), Asparagine synthase, glutamine-hydrolyzing (InterPro:IPR006426), Glutamine amidotransferase, type II (InterPro:IPR017932); BEST Arabidopsis thaliana protein match is: asparagine synthetase 2 (TAIR:AT5G65010.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR