K25612

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K25612

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T1  
cauline leaf (PO:0000013)
  • decreased size (PATO:0000587)
    (Cauline leaf::Shape::small)
rosette leaf (PO:0000014)
  • increased size (PATO:0000586)
    (Rosette leaf::Shape::large)
  • epinastic (PATO:0000945)
    (Rosette leaf::Shape::epinastic)
stem (PO:0009047)
  • abnormal (PATO:0000460)
    (Stem::Others)
T2  
cauline leaf (PO:0000013)
  • increased size (PATO:0000586)
    (Cauline leaf::Shape::large)
  • decreased size (PATO:0000587)
    (Cauline leaf::Shape::small)
  • decreased width (PATO:0000599)
    (Cauline leaf::Shape::narrow)
  • epinastic (PATO:0000945)
    (Cauline leaf::Shape::epinastic)
  • dark green (PATO:0001249)
    (Cauline leaf::Color::dark)
rosette leaf (PO:0000014)
  • increased size (PATO:0000586)
    (Rosette leaf::Shape::large)
  • increased length (PATO:0000573)
    (Rosette leaf::Shape::long)
  • epinastic (PATO:0000945)
    (Rosette leaf::Shape::epinastic)
fruit (PO:0009001)
  • abnormal (PATO:0000460)
    (Silique::Others)
stem internode (PO:0020142)
  • decreased length (PATO:0000574)
    (Stem::Shape::short internode)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K25612

Determination of introduced cDNA(s)

  • J033098G23 - Rice Full-length cDNAs - Original: J033098G23

    full reading [+] show sequence

    Nucleotide sequence: J033098G23 - full reading 
    GGGACTCCTC TTCTCTTCTC TTCTCTTCTC AGAGCGCTGC GAGGTTGCCA 
    TGGACGAGGC GGAGGCGGCG GCGGCGGCGG CGAAGATGGA CGAGCTGGCG 
    GGAGGAGGAG GAGGAGGCGG CGGCGACTGG TCGTACCTCG CGGCGGACGC 
    GCTGGCGGCG GCGTCGTTCA CGGCGTTCCC GTTCCACCAC CACCACCACC 
    ACCACCACCG CGACGTGCTC TCGGCGTCGA CCCCGTCGTC GCTGCTCCTC 
    AACATGGACG CGGCCACCGC CGCGGCCATG TTCGACTTCC AGGCGGCCTT 
    CCCGTCGTCC TCCGTCCCGC CGCCGCCGCC CACGACGACG GCGGCGCTCC 
    CGCCGTTCCA CGACTTCGCC TCCTCCAACC CGTTCGACGA CGCGCCGCCC 
    CCGTTCCTCG CGCCGCCGGG GCAGAAGCTC GGGTTCCTGG GGCCCCCCGG 
    CGGCGCGTTC GGCGGCGGGA TGGGGTGGGA CGACGACGAC GAGATCGAGC 
    AGAGCGTGGA CGCCTCGTCC ATGGGTGTCT CGGCCTCGCT GGAGAATGCC 
    GCGCCCGTGG CGGCCGGAGG AGGCGGCGGC GGTGGCGGCG GCGGTGGGAG 
    GGGCAAGAAG AAGGGGATGC CGGCCAAGAA CCTCATGGCG GAGCGGCGGC 
    GCAGGAAGAA GCTCAACGAC CGCCTCTACA TGCTGCGCTC CGTGGTGCCC 
    AAGATCAGCA AGATGGACAG AGCTTCTATC CTTGGTGATG CAATTGAGTA 
    CTTGAAGGAG CTCCTGCAGA GGATTAACGA TCTTCACAAT GAACTTGAGT 
    CTGCTCCTAG CTCTTCGCTT ACTGGACCAT CATCGGCTAG CTTCCACCCA 
    TCAACACCCA CATTGCAGAC GTTTCCTGGC CGCGTTAAGG AGGAACTTTG 
    CCCAACCTCA TTTCCAAGCC CTAGTGGTCA ACAAGCCACG GTCGAAGTTA 
    GGATGAGGGA AGGTCATGCA GTCAATATCC ACATGTTCTG CGCACGCAGG 
    CCCGGCATTC TGATGTCCAC ATTGAGGGCT CTCGACAGCC TTGGCCTCGG 
    GATCGAGCAG GCGGTCATCA GCTGTTTCAA TGGGTTTGCA ATGGATGTTT 
    TCCGCGCCGA GCAATGCAGG GATGGTCCTG GACTCGGGCC TGAAGAAATT 
    AAGACCGTGC TCCTGCACTC TGCTGGCCTT CAGAACGCAA TGTAATCAGC 
    AAGTCAGAAA GCAGTCAAGA CCAATGCACT GGGATTTGAT CAGTGATCAT 
    TTGCATCAGA TGATCTGGTT TATCAAAAAG TTATAGTAAG TATGGCATAT 
    ATGCTGTTTA TCTAGGAGAA CTGATGAGAT GAGGCTCGCC ATCTAGAGAC 
    ATGTGTGATG ATGATGATGT AACTGGGTTT CTTTCTTTCT GTTGTCGTTT 
    TTTTTTCTGA ATGTTGTTTG TGATGTAATA GGATCTTTGT CCATTGAAAC 
    CATGTCCATT ATCATGATGA GAATATGAAC CCAGTGTCTT TTG

    Gene models with high sequence identity

    1. AT3G26744.4 [+] show detail - basic helix-loop-helix (bHLH) DNA-binding superfamily protein
      E-value: 4e-74; Score: 275.00

     

    InterPro Scan Digest

    Program Description E-value
    HMMSmart Helix-loop-helix DNA-binding domain 6.2e-14
    Coil coiled-coil 0
    ProfileScan Helix-loop-helix DNA-binding domain 13.088
    superfamily Helix-loop-helix DNA-binding 1.6e-16
    Gene3D Helix-loop-helix DNA-binding 1.5e-05
    HMMPfam Helix-loop-helix DNA-binding domain 1.7e-09

    See the detailed result >

    AT3G26744.4

    Model type
    Protein coding
    Short Description
    basic helix-loop-helix (bHLH) DNA-binding superfamily protein
    Curator Summary
    Encodes a MYC-like bHLH transcriptional activator that binds specifically to the MYC recognition sequences in the CBF3 promoter. Mutants are defective in cold-regulated gene expression. Cold stress triggers protein degradation of nuclear GFPICE1 protein, and the RING finger protein HOS1 is required. Sumoylation of ICE1 controls CBF3/DREB1A expression and freezing tolerance.
    Computational Description
    INDUCER OF CBF EXPRESSION 1 (ICE1); CONTAINS InterPro DOMAIN/s: Helix-loop-helix DNA-binding domain (InterPro:IPR001092), Helix-loop-helix DNA-binding (InterPro:IPR011598); BEST Arabidopsis thaliana protein match is: basic helix-loop-helix (bHLH) DNA-binding superfamily protein (TAIR:AT1G12860.1); Has 2623 Blast hits to 2616 proteins in 144 species: Archae - 0; Bacteria - 0; Metazoa - 19; Fungi - 30; Plants - 2572; Viruses - 0; Other Eukaryotes - 2 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR