K25912

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K25912

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage
Phenotypes
T2 seed (PO:0009010)
  • increased width (PATO:0000600)
    (Seed::Shape::wide)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K25912

Determination of introduced cDNA(s)

  • J013021F09 - Rice Full-length cDNAs - Original: J013021F09

    full reading [+] show sequence

    Nucleotide sequence: J013021F09 - full reading
    TCTCCTCCCG CCGCCGCCGC ACGCGGAACC CTAGAAAACC CTAGCAGCGG 
    CGAGCACCGG GGAGCGGGCG GCCATGGCGG AGCACGACCT GACGGCGCGG
    ATGGCGGGGC AGATGGACTG CCACCTGGTG TTCCCGCTGC TGGAGTTCCT
    GCAGGAGCGT GCGCTGTACG CGAACAAGGA GATCCTGGAG GCGAAGCTCC
    GCCTCCTCAG CGGGACCAAC ATGGTGGACT ACGCCATGGA CATCCACAAG
    TCCCTCCACG ACACCGACGA GGTCCCCGAC GACATGGTCA GGCGCCGCAC
    CGACGTCGTC TCCAGGCTCA GGGCGCTCGA CGAGGCCACC GCCCCCATCG
    TCTCCTTCCT CCAGAACCAG CAGCTCGTCC AGGAGCTCAG GCCAGACAAG
    CAGTACAACC TCCACATGCT CCAGGACCGC TTCGAGATTG GTCCTGAGCA
    GATTGAGACT CTGTATCAGT TTGCCAAGTT TCAGTTTGAC TGCGGTAATT
    ACTCAGATGC TGCTGTCTAC TTGTATCAAT ACCGTGCTTT GTGCACGAAC
    AGCGAGAGGA GCTTGAGTGC CCTGTGGGGA AAGCTGGCAG CCGAGATTTT
    GATGCAGAAC TGGGATGTTG CACTGGATGA GCTCAACCGT TTGAAGGAGA
    TTATTGATTC AAAGAACTTC TCATCACCAC TAAATCAGCT CCAGAATAGG
    ATATGGCTGA TGCATTGGAG TATTTTTATC TTCTTTAACC ATGAAAACGG
    CAGAAATGGG ATCATCGATC TGTTCTTCCA GGATAGGTAC TTGAACGCAA
    TCCAGACAAA TGCACCCCAT CTTCTGAGAT ACCTTGCTAC AGCAGTTGTC
    GTGAACAAAA GGAGAAGGAA CATGCTTAAA GAGTTGATTA AAGTCATTCA
    GCAGGAGCAG CACAGCTACA AGGATCCCAT CACTGAATTC TTGGAATGCT
    TGTATGTTAA CTATGATTTT GATGGTGCAC AACAGAAACT CATAGAGTGT
    GAGCAGGTCA TATTGAATGA TCCTTTCCTT GGAAAGCGCA TTGGGGAAGG
    GAATTTTGTC ACTGTTCCTT TGAGAGATGA ATTCCTTGAA AATGCTCGTC
    TGTTTATCTT TGAAACTTAC TGCCGTATTC ATCGGTGCAT TGATATTGGC
    ATGCTTTCTC AGAAGCTGAA CATGAGCTAC GATGAGGCAG AATTGTGGAT
    TATGAACTTA GTTAGGAATT CAAAGCTCGA CGCCAAGATT GATTCAGTGT
    CTGGAACTCT TATCATGACG ACCAATCATG TTAACATCCA TGAGCAGGTT
    ATCGAAAGCT TAAAGAATCT TAACATGCGG ACTTTCCTGC TAGCAAAGAA
    CATTGTGGAG CCGGCTCAAG CAGCACAACA GGCAGCTCGG TGAGATTGAC
    AGAGAAGACC ACAGTTTTGA GGCGCCCGGA ATTATTTAAG TAGAGACAAT
    CCTCTAAACT TTTGGAGTTT TGTTGTTTAG CATTGACTCC GTTATCAGTA
    CCTAAGATCA TCTAGGAGGT GGTTTCTCGT TCGCTACAGC ATATGCCGAC
    GTTTTCTTTC TATTTTGTAC TTGGAATCCT GTGGCTGAAG TTAATCCTTT
    CGTTGGTACT ACAATCAAGT ATTACCTGAT TGTTATGGTG TTTCGTGTTG
    AAGTGAAGTG CGGAAGTGTG GTCATTTTTA TAATTGTAGG TTCAATGTTT
    TTCTTGGTTC TGCTAAATTT GTGGCGCCAC ATTTTACCTT GATTTCAGTC
    AATTATTTGA CC

    Gene models with high sequence identity

    1. AT3G57290.1 [+] show detail - eukaryotic translation initiation factor 3E
      E-value: 0; Score: 634.00

     

    InterPro Scan Digest

    Program Description E-value
    Coil coiled-coil 0
    superfamily "Winged helix" DNA-binding domain 9.3e-16
    HMMPIR Translation initiation factor 3, subunit 6, eukaryotic 2.9e-264
    HMMPanther EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SU 4.1e-204
    HMMPfam Eukaryotic translation initiation factor 3 (eIF3), subunit 6, N-terminal 3.2e-43
    HMMPfam Proteasome component (PCI) domain 1.4e-16
    HMMSmart Proteasome component (PCI) domain 2e-13

    See the detailed result >

    AT3G57290.1

    Model type
    Protein coding
    Short Description
    eukaryotic translation initiation factor 3E
    Curator Summary
    Encodes a protein that is found in not only the eif3 complex but also in association with subunits of the COP9 signalosome. eIF3e appears to be subjected to proteasome-dependent degradation that requires the PCI domain of eIF3e. The level of eIF3e present in cells appears to affect the rate of translation.
    Computational Description
    eukaryotic translation initiation factor 3E (EIF3E); FUNCTIONS IN: protein binding, translation initiation factor activity; INVOLVED IN: flower development, response to salt stress, translation, photomorphogenesis, transcription initiation; LOCATED IN: signalosome, eukaryotic translation initiation factor 3 complex, nucleus, plasma membrane, cytoplasm; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Translation initiation factor 3, subunit 6, eukaryotic (InterPro:IPR016650), Proteasome component (PCI) domain (InterPro:IPR000717), Eukaryotic translation initiation factor 3 (eIF3), subunit 6, N-terminal (InterPro:IPR019010); Has 641 Blast hits to 638 proteins in 243 species: Archae - 0; Bacteria - 2; Metazoa - 287; Fungi - 119; Plants - 138; Viruses - 0; Other Eukaryotes - 95 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR