K25912

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Phenotypes

Morphological phenotypes

Generation	Anatomical entity /structure development stage	Phenotypes
T2	seed (PO:0009010)	increased width (PATO:0000600) (Seed::Shape::wide)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K25912

Determination of introduced cDNA(s)

J013021F09 - Rice Full-length cDNAs - Original: J013021F09

full reading [+] show sequence

TCTCCTCCCG CCGCCGCCGC ACGCGGAACC CTAGAAAACC CTAGCAGCGG 
CGAGCACCGG GGAGCGGGCG GCCATGGCGG AGCACGACCT GACGGCGCGG 
ATGGCGGGGC AGATGGACTG CCACCTGGTG TTCCCGCTGC TGGAGTTCCT 
GCAGGAGCGT GCGCTGTACG CGAACAAGGA GATCCTGGAG GCGAAGCTCC 
GCCTCCTCAG CGGGACCAAC ATGGTGGACT ACGCCATGGA CATCCACAAG 
TCCCTCCACG ACACCGACGA GGTCCCCGAC GACATGGTCA GGCGCCGCAC 
CGACGTCGTC TCCAGGCTCA GGGCGCTCGA CGAGGCCACC GCCCCCATCG 
TCTCCTTCCT CCAGAACCAG CAGCTCGTCC AGGAGCTCAG GCCAGACAAG 
CAGTACAACC TCCACATGCT CCAGGACCGC TTCGAGATTG GTCCTGAGCA 
GATTGAGACT CTGTATCAGT TTGCCAAGTT TCAGTTTGAC TGCGGTAATT 
ACTCAGATGC TGCTGTCTAC TTGTATCAAT ACCGTGCTTT GTGCACGAAC 
AGCGAGAGGA GCTTGAGTGC CCTGTGGGGA AAGCTGGCAG CCGAGATTTT 
GATGCAGAAC TGGGATGTTG CACTGGATGA GCTCAACCGT TTGAAGGAGA 
TTATTGATTC AAAGAACTTC TCATCACCAC TAAATCAGCT CCAGAATAGG 
ATATGGCTGA TGCATTGGAG TATTTTTATC TTCTTTAACC ATGAAAACGG 
CAGAAATGGG ATCATCGATC TGTTCTTCCA GGATAGGTAC TTGAACGCAA 
TCCAGACAAA TGCACCCCAT CTTCTGAGAT ACCTTGCTAC AGCAGTTGTC 
GTGAACAAAA GGAGAAGGAA CATGCTTAAA GAGTTGATTA AAGTCATTCA 
GCAGGAGCAG CACAGCTACA AGGATCCCAT CACTGAATTC TTGGAATGCT 
TGTATGTTAA CTATGATTTT GATGGTGCAC AACAGAAACT CATAGAGTGT 
GAGCAGGTCA TATTGAATGA TCCTTTCCTT GGAAAGCGCA TTGGGGAAGG 
GAATTTTGTC ACTGTTCCTT TGAGAGATGA ATTCCTTGAA AATGCTCGTC 
TGTTTATCTT TGAAACTTAC TGCCGTATTC ATCGGTGCAT TGATATTGGC 
ATGCTTTCTC AGAAGCTGAA CATGAGCTAC GATGAGGCAG AATTGTGGAT 
TATGAACTTA GTTAGGAATT CAAAGCTCGA CGCCAAGATT GATTCAGTGT 
CTGGAACTCT TATCATGACG ACCAATCATG TTAACATCCA TGAGCAGGTT 
ATCGAAAGCT TAAAGAATCT TAACATGCGG ACTTTCCTGC TAGCAAAGAA 
CATTGTGGAG CCGGCTCAAG CAGCACAACA GGCAGCTCGG TGAGATTGAC 
AGAGAAGACC ACAGTTTTGA GGCGCCCGGA ATTATTTAAG TAGAGACAAT 
CCTCTAAACT TTTGGAGTTT TGTTGTTTAG CATTGACTCC GTTATCAGTA 
CCTAAGATCA TCTAGGAGGT GGTTTCTCGT TCGCTACAGC ATATGCCGAC 
GTTTTCTTTC TATTTTGTAC TTGGAATCCT GTGGCTGAAG TTAATCCTTT 
CGTTGGTACT ACAATCAAGT ATTACCTGAT TGTTATGGTG TTTCGTGTTG 
AAGTGAAGTG CGGAAGTGTG GTCATTTTTA TAATTGTAGG TTCAATGTTT 
TTCTTGGTTC TGCTAAATTT GTGGCGCCAC ATTTTACCTT GATTTCAGTC 
AATTATTTGA CC

Gene models with high sequence identity

AT3G57290.1 [+] show detail - eukaryotic translation initiation factor 3E
E-value: 0; Score: 634.00

InterPro Scan Digest

Program	Description	E-value
Coil	coiled-coil	0
superfamily	"Winged helix" DNA-binding domain	9.3e-16
HMMPIR	Translation initiation factor 3, subunit 6, eukaryotic	2.9e-264
HMMPanther	EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SU	4.1e-204
HMMPfam	Eukaryotic translation initiation factor 3 (eIF3), subunit 6, N-terminal	3.2e-43
HMMPfam	Proteasome component (PCI) domain	1.4e-16
HMMSmart	Proteasome component (PCI) domain	2e-13

See the detailed result >

AT3G57290.1

Model type: Protein coding
Short Description: eukaryotic translation initiation factor 3E
Curator Summary: Encodes a protein that is found in not only the eif3 complex but also in association with subunits of the COP9 signalosome. eIF3e appears to be subjected to proteasome-dependent degradation that requires the PCI domain of eIF3e. The level of eIF3e present in cells appears to affect the rate of translation.
Computational Description: eukaryotic translation initiation factor 3E (EIF3E); FUNCTIONS IN: protein binding, translation initiation factor activity; INVOLVED IN: flower development, response to salt stress, translation, photomorphogenesis, transcription initiation; LOCATED IN: signalosome, eukaryotic translation initiation factor 3 complex, nucleus, plasma membrane, cytoplasm; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Translation initiation factor 3, subunit 6, eukaryotic (InterPro:IPR016650), Proteasome component (PCI) domain (InterPro:IPR000717), Eukaryotic translation initiation factor 3 (eIF3), subunit 6, N-terminal (InterPro:IPR019010); Has 641 Blast hits to 638 proteins in 243 species: Archae - 0; Bacteria - 2; Metazoa - 287; Fungi - 119; Plants - 138; Viruses - 0; Other Eukaryotes - 95 (source: NCBI BLink).
Link: InterPro Scan - TAIR