K26346

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K26346

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T2 seed (PO:0009010)
  • decreased length (PATO:0000574)
    (Seed::Shape::short)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K26346

Determination of introduced cDNA(s)

  • J023112B16 - Rice Full-length cDNAs - Original: J023112B16

    full reading [+] show sequence

    Nucleotide sequence: J023112B16 - full reading 
    GAATCGAGTG CAAGAAGAGG AAAGCAAGAC GCGTCAAATC TATCAATCAG 
    CTTCAGCCAA AGCCGAGAGC CCCCCCAAAA GAGAGAGAAG CACGCATCCG 
    ATCGCCGGCG ACGACCGAAT CTGTGCCGGG CGGCAGGAAG GAGAGCGGCG 
    ATGGCAGCGA TGGCATCCCT GTACCGGCGT GTCCTTCCTT CGCCGCCGGC 
    TGTGGAGTTC GCGTCGGAGG AGGGGAAGCG GCTGTTCTCG GAGGCCCTGG 
    AGAGCGGGAC CTTGCAAGGC TTCTTCAACC TCATCTCCGT GTTCCAGACG 
    CAGTCGGAGC CGGCCTTCTG CGGCCTCGCC TCCCTCTCCG TCGTCCTCAA 
    CGCCCTCGCC ATCGACCCGG GCCGCCAATG GAAGGGCCCC TGGAGGTGGT 
    TCGACGAGTC CATGCTTGAC TGCTGCGAGC CCCTCGACAA GGTGAAGGCG 
    GAGGGCATCA CCTTCGCCAA ACTCGCCTGC CTCGCGCACT GCGCCGGTGC 
    CAATGTCCGC TCCTTCCGCG CCGACCAGTC CACCATCCAC GACTTCCGCC 
    ACCATCTCGT CCGCTCTGCC TCCTCCCAGG ACTGCCATCT CATCACATCC 
    TACCACAGGA AGCCTTTCAA ACAACTGGAA CCGGCCATTT CTCTCCAATC 
    GGCGGCTACC ATGCCGGCCA AGACATGGCG CTTATCCTGG ATGTCGCCCG 
    CTTCAAATAC CCTCCTCACT GGGTTCCACT CCCACTGCTT TGGGAAGCCA 
    TGAATACAAC TGATGACGCA ACTGGTCTAC TCAGGGGGTT CATGCTTATC 
    TCAAGGCACA CTGCAGCTCC TTCATTGCTC TACACAGTGC TTTTGTGCAG 
    AGTTGCAGAG ATGAAAGCTG GAAAAGCATG GCGAAGTATT GCATGGAAGA 
    TGTACCCGAT CTTCTTAAGG ATGAGAGTGT AGACAATGTT CCAGCACTTC 
    TGTCCCGCTT AGTGAAATCC CTTCCTGCCA ATGCTGGAAA TTTGATCAAA 
    TGGGTTATTG AAGTTAGGAG ACAAGAGGAA GGAGGATCAG GATTAAGCAA 
    AGAGGAGGAA GAAAGGCTTA TTTTGAAGGA AATGATACTA CAGCAAGTCC 
    GTGATACTGA GCTTTTTAGA TTAGTCCGTG AACTGCAATT CACTAAGCAG 
    CCATGTTGTA GTTGCTCATA TTCAAGTGAT GATGATTCCT TTACCCGGAT 
    TGCAGCCTCT GTGTGCTGTC AAGGGGCCGC ATTGCTAACA GGGAATCTTT 
    CATCAAAAGA TGGGTTCTGC TGCAGAGAAA CTTGCTTCAA ATGTGTACAA 
    GTGGATGGTG ATGGGCCTAA GACTGTCGTT ACAGGCACAG CGGTTTCAGG 
    AGTCAATGAA CAAAGTGTTG ATATGCTTCT ACCGATATCC ACATTGGAAA 
    CAAGCGTGTG CAATTCAAAT TCAAGCAACG AGGTTGTCAA ATATCCATCT 
    AGAACAGATA TTTTAACTGT TCTATTGCTG GCTTTACATC CTAGCACATG 
    GGTGGGCATT AAAGACGAGA GGCTGAAAGC TGAATTCCAG AGTCTTATTT 
    CAACAGACAT TCTTCATGAT GATCTTAAAC GAGAGATATT GCATCTAAGA 
    CGGCAACTCC ATTATGTGAG GTCCTGTAAA GAGGAGGAAT ATGGAGATCC 
    TGTGCCACAA TCCCATTAAC AATGATGCAA ATCGCGCAGT TGGTTACCCT 
    GGAGATGCAA AAAAAAGGGG TTAGAGGAGG AACTACATAC TCCGTATTAC 
    CTTTGTTTCG AGTGAGGACT TCTCATTTTT GAGACACCTG ACCTGAGACG 
    GATCCGTGTA GACATGTTCA TGTTCATCAC CTGTGGTCGT TTCTCTTGTT 
    AGTGACAACT GACAACTAGC GGGGAGGCAC ACGCTAATTG TGCGGCTGGT 
    GTCCTTGCAA AAGTTTCTCA TAATAACGCA ATGGCAGATA TATTTTCACA 
    CTTTATTATT AAATAAAAAT ATTGAAAAGG GCAATTAAGG AGTAGGATAA 
    ACTCTAAG

    Gene models with high sequence identity

    1. AT5G44070.1 [+] show detail - phytochelatin synthase 1 (PCS1)
      E-value: 4e-137; Score: 120.00

     

    InterPro Scan Digest

    Program Description E-value
    HMMPfam Phytochelatin synthase 1.1e-51
    HMMPfam Phytochelatin synthase, C-terminal 1.2e-113
    HMMPfam Phytochelatin synthase 4.6e-30
    ProfileScan Phytochelatin synthase 31.446
    ProfileScan Phytochelatin synthase 17.493

    See the detailed result >

    AT5G44070.1

    Model type
    Protein coding
    Short Description
    phytochelatin synthase 1 (PCS1)
    Curator Summary
    Phytochelatin synthase gene confers tolerance to cadmium ions. Catalyzes phytochelatin (PC) synthesis from glutathione (GSH) in the presence of Cd2+, Zn2+, Cu2+ and Fe3+, but not by Co2+ or Ni2+.
    Computational Description
    CADMIUM SENSITIVE 1 (CAD1); FUNCTIONS IN: cadmium ion binding, copper ion binding, glutathione gamma-glutamylcysteinyltransferase activity; INVOLVED IN: in 6 processes; LOCATED IN: cytosol; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Phytochelatin synthase, C-terminal (InterPro:IPR015407), Phytochelatin synthase (InterPro:IPR007719); BEST Arabidopsis thaliana protein match is: phytochelatin synthase 2 (TAIR:AT1G03980.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR