K27343

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K27343

Phenotypes

Morphological phenotypes

Specific phenotypess were NOT observed.

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K27343

Determination of introduced cDNA(s)

  • J013122O17 - Rice Full-length cDNAs - Original: J013122O17

    full reading [+] show sequence

    Nucleotide sequence: J013122O17 - full reading
    GCCCCTCTCT CTCCTTCAAA GCAGGAGAAA AGAAGAAAAA TTGATTGCTC 
    TCTCGTTCCG TTCCGGAGGA GAGAGAGAGA GAGAGAGAGA GGAGAGCGGC
    GGCGATGGCG TCGTCGTCGT CGTCGGCGTC GAGCAGGTTC TCGCCGGCGC
    TGCAGGCGTC GGATCTCAAC GACTTCATCG CCCCCTCGCA GGACTGCATC
    ATCTCCCTCA ACAAGGGCCC CTCCGCCCGC CGCCTTCCGA TCAAACAAAA
    GGAAATTGCA GTGAGCACAA ATCCTCCAGA GGAAGCAGTC AAGATTTCAC
    TGAAGGACTG TTTGGCTTGC AGTGGTTGCA TAACATCAGC AGAAACAGTT
    ATGCTTGAGA AGCAAAGCTT GGGTGACTTC ATTACTCGCA TCAACTCTGA
    CAAAGCTGTC ATAGTATCGG TCTCTCCACA GTCAAGAGCA TCGCTAGCTG
    CATTCTTTGG TCTTTCTCAA TCACAGGTTT TCAGAAAACT CACTGCGCTG
    TTCAAGTCGA TGGGCGTAAA GGCAGTTTAC GACACAAGTT CCAGTCGAGA
    TCTGTCCCTA ATTGAAGCAT GCAGTGAATT TGTTACTCGT TATCATCAAA
    ACCAGTTATC TAGTGGCAAA GAAGCTGGAA AAAATCTTCC CATGCTTTCA
    TCTGCATGCC CAGGTTGGAT ATGCTATGCA GAAAAGACTC TCGGTTCATT
    TATCTTGCCT TACATCTCAG CTGTGAAGAG CCCCCAACAG GCCATTGGTG
    CTGCTATCAA GCATCATATG GTTGGAAAAC TTGGTCTGAA GCCACATGAT
    GTTTATCATG TTACTGTGAT GCCCTGTTAT GATAAAAAAC TAGAAGCTGT
    TAGGGATGAT TTTGTTTTCT CAGTGGAAGA TAAAGATGTT ACAGAGGTAG
    ATTCCGTATT AACCACTGGT GAAGTGTTAG ATTTGATACA GTCCAGATCT
    GTTGATTTCA AGACCCTAGA GGAGTCTCCC ATGGACAGAT TATTAACCAA
    TGTTGATGAT GACGGTCAAC TATATGGGGT TTCTGGTGGC TCAGGGGGTT
    ATGCAGAAAC AGTCTTTCGA CATGCGGCAC ATGTTCTCTT CGATAGAAAA
    ATTGAAGGAT CAGTTGATTT CAGAATCTTA CGAAATTCAG ATTTTCGTGA
    GGTTACGCTG GAGGTGGAGG GCAAACCTGT TCTTAAGTTT GCACTTTGTT
    ATGGATTCAG AAATCTGCAG AACATTATTA GGAAGATTAA GATGGGAAAA
    TGCGAATATC ACTTTATTGA AGTTATGGCA TGTCCTTCAG GCTGCCTGAA
    TGGGGGAGGT CAAATAAAAC CTGCCAAAGG CCAGTCTGCG AAGGATCTTA
    TCCAACTACT AGAGGATGTG TACATCCAAG ATGTATCGGT ATCCAACCCC
    TTCGAGAACC CTATTGCGAA AAGATTATAT GATGAATGGC TTGGGCAACC
    TGGTTCAGAA AACGCAAAGA AGTATCTACA CACGAAGTAT CACCCAGTGG
    TGAAAAGCGT GGCATCGCAG TTGCAGAATT GGTGATAACA CAGTGGTGGA
    AAGCATTTCC CAGTGTGGGC AGTTGCTTGT GCCATCTTGG TGCCCTGCCC
    ACACTAAGCA AACACTGAAC ACACAGAAAA GAAATACACG TCATCAAAGA
    TTTTGCATGC GGGCAAATGA ATTTTGTTAG ATTGTAGGTT TTACCAGCTT
    TTCTTGGTAA TGTTTGTATA CTACCTCCTC ACTGGGAAAC ACAGATGAGA
    TTGTAACATG TAAAAACGAA GTGAACCAAA TAGTAGTTGA TCTACTCAGC
    TG

    Gene models with high sequence identity

    1. AT4G16440.1 [+] show detail - ferredoxin hydrogenases
      E-value: 3e-172; Score: 602.00

     

    InterPro Scan Digest

    Program Description E-value
    HMMSmart Iron hydrogenase, small subunit-like 0.00000000028
    superfamily Iron hydrogenase 2.1e-127
    HMMPfam Iron hydrogenase, large subunit, C-terminal 7.5e-93
    HMMPfam Iron hydrogenase, small subunit-like 0.00000000018
    Gene3D no description 6.5e-45
    Gene3D Iron hydrogenase, small subunit-like 0.00028
    HMMPanther NARF-RELATED 1.5e-169
    HMMPanther NITRATE, FROMATE, IRON DEHYDROGENASE 1.5e-169

    See the detailed result >

    AT4G16440.1

    Model type
    Protein coding
    Short Description
    ferredoxin hydrogenases
    Curator Summary
    Encodes a [FeFe]-hydrogenase-like protein named Gollum (for Growth in different Oxygen LeveLs inflUences Morphogenesis). Heterologous expression of Gollum in E. coli indicates that it probably contains two [Fe-S] clusters with different magnetic properties. Sequence alignment analysis indicates that these two clusters would be topologically equivalent to the mesial and proximal [Fe-S] centers of [FeFe]-hydrogenases. Knockdown mutants (RNAi) show a dwarf phenotype at the normal atmospheric partial oxygen pressure of 21 kPa. This dwarf phenotype could be rescued by growing the plant under low oxygen pressure (5kPa), suggesting a role for this gene in oxygen sensing.
    Computational Description
    ferredoxin hydrogenases; CONTAINS InterPro DOMAIN/s: Iron hydrogenase, large subunit, C-terminal (InterPro:IPR004108), Iron hydrogenase (InterPro:IPR009016), Iron hydrogenase, small subunit-like (InterPro:IPR003149); Has 2574 Blast hits to 2544 proteins in 612 species: Archae - 1; Bacteria - 1826; Metazoa - 221; Fungi - 188; Plants - 72; Viruses - 0; Other Eukaryotes - 266 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR