K27603

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K27603

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T1  
cauline leaf (PO:0000013)
  • decreased size (PATO:0000587)
    (Cauline leaf::Shape::small)
rosette leaf (PO:0000014)
  • decreased size (PATO:0000587)
    (Rosette leaf::Shape::small)
  • decreased length (PATO:0000574)
    (Rosette leaf::Shape::short)
  • increased width (PATO:0000600)
    (Rosette leaf::Shape::wide)
  • abnormal (PATO:0000460)
    (Rosette leaf::Others)
stem (PO:0009047)
  • abnormal (PATO:0000460)
    (Stem::Others)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K27603

Determination of introduced cDNA(s)

  • J023110H16 - Rice Full-length cDNAs - Original: J023110H16

    full reading [+] show sequence

    Nucleotide sequence: J023110H16 - full reading 
    GGTCTTCCAT TCCAGCCCAG ATCGAGCGAG AGAGCCGTCT CCCTCTTCGC 
    TTCTCCTCTC CCCCCCGTGA TCTGACCGCC GGCGACGACC CCCTCCCACC 
    ACCCGCCGCC GCCGCCGCGC CCCCGCCCCC GCCGGCGACG ACCACCATGT 
    CGTACGATCG CGTCACGACC TTCGAGGACT CGGAGAAGGA GAGCGAGTAC 
    GGCTACGTCC GCAAGGTTTC TGGACCTGTG GTCGTGGCTG ATGGAATGGG 
    TGGTGCTGCC ATGTATGAAC TTGTCCGAGT TGGTAATGAT AACCTCATTG 
    GGGAAATTAT TCGTCTTGAG GGTGATTCAG CCACCATTCA AGTTTATGAG 
    GAAACAGCTG GGTTGATGGT CAATGATCCA GTGTTGAGAA CAAGAAAGCC 
    TCTTTCAGTT GAATTGGGAC CTGGTATCCT TGGAAATATC TTTGATGGAA 
    TCCAGCGCCC TCTAAAAACT ATTGCTATAA AGTCTGGAGA TGTGTACATA 
    CCACGTGGTG TTTCAGTCCC TGCTCTTGAC AAAGATCAGT TGTGGGATTT 
    TGAGCCAAAG AAGCTAGGTG TTGGAGATGC CATCACTGGT GGAGATCTAT 
    ATGCTACTGT GTTTGAGAAT ACGCTGATGA AACATCATGT TGCCCTTCCT 
    CCTGGTTCTA TGGGGAAAAT TAGTTACATT GCACCAGCTG GCCAGTATAG 
    CTTGCAGGAT ACAGTTCTGG AACTGGAATT CCAGGGCATC

    Gene models with high sequence identity

    1. AT1G78900.2 [+] show detail - vacuolar ATP synthase subunit A
      E-value: 2e-93; Score: 338.00

     

    InterPro Scan Digest

    Program Description E-value
    HMMPanther V-TYPE ATP SYNTHASE ALPHA CHAIN 3.6e-69
    HMMPanther ATP SYNTHASE 3.6e-69
    superfamily ATPase, F1/A1 complex, alpha/beta subunit, N-terminal 2.3e-19
    HMMPfam ATPase, F1/V1/A1 complex, alpha/beta subunit, N-terminal 1.6e-14

    See the detailed result >

    AT1G78900.2

    Model type
    Protein coding
    Short Description
    vacuolar ATP synthase subunit A
    Curator Summary
    Encodes catalytic subunit A of the vacuolar ATP synthase. Mutants are devoid of vacuolar ATPase activity as subunit A is encoded only by this gene and show strong defects in male gametophyte development and in Golgi stack morphology.
    Computational Description
    vacuolar ATP synthase subunit A (VHA-A); FUNCTIONS IN: hydrogen ion transporting ATP synthase activity, rotational mechanism, hydrolase activity, acting on acid anhydrides, catalyzing transmembrane movement of substances, proton-transporting ATPase activity, rotational mechanism, ATP binding; INVOLVED IN: response to salt stress, proton transport, Golgi organization, pollen development; LOCATED IN: in 8 components; EXPRESSED IN: 29 plant structures; EXPRESSED DURING: 17 growth stages; CONTAINS InterPro DOMAIN/s: ATPase, alpha/beta subunit, nucleotide-binding domain, active site (InterPro:IPR020003), ATPase, F1/V1/A1 complex, alpha/beta subunit, C-terminal (InterPro:IPR000793), ATPase, F1/V1/A1 complex, alpha/beta subunit, N-terminal (InterPro:IPR004100), ATPase, F1/A1 complex, alpha/beta subunit, N-terminal (InterPro:IPR018118), ATPase, alpha/beta subunit, nucleotide-binding domain (InterPro:IPR000194), ATPase, V1 complex, subunit A (InterPro:IPR005725); BEST Arabidopsis thaliana protein match is: ATP synthase alpha/beta family protein (TAIR:AT5G08670.1); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR