K27638

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K27638

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T1  
whole plant (PO:0000003)
  • increased height (PATO:0000570)
    (Adult plant::Plant height::tall)
cauline leaf (PO:0000013)
  • dark green (PATO:0001249)
    (Cauline leaf::Color::dark)
rosette leaf (PO:0000014)
  • increased size (PATO:0000586)
    (Rosette leaf::Shape::large)
  • dark green (PATO:0001249)
    (Rosette leaf::Color::dark)
root (PO:0009005)
  • present in greater numbers in organism (PATO:0000470)
    (Root::Number::many)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K27638

Determination of introduced cDNA(s)

  • J023111A19 - Rice Full-length cDNAs - Original: J023111A19

    full reading [+] show sequence

    Nucleotide sequence: J023111A19 - full reading 
    GATACTTAGT ACTGTACGTA CTAAACCGGG GATTGATCAG GAGAGTGCAG 
    TGCAAACTCG CAGTGAACCT GTGTGATCGA TCGGCCATGG AGTCGCTGCT 
    CGCAGCCGGT GCGGGAGGGA TCGGCGTGGC TGCGGCGGCC GCCGTCGTGG 
    CGGCGACCCT CGCCGTGGTG CCGCCCAAGG ACCGCGGCAA CAACCCGCCT 
    CCAGCTGTTC CTGGTTTACC CGTTATTGGA AATATGCATC AATTGAAAGA 
    AAAGAAGCCT CATCATACCT TTACAAAATG GTCTAAAACT TATGGGCCAA 
    TCTACACTAT AAAGACCGGA GCTTCTTCAG TAGTTGTGCT CAATTCAACG 
    GAAGTAGCCA AGGAGGCGAT GATTGAAAAA TTCTCATCAA TATCAACTAA 
    AAAGCTACCA AAAGCATTGT CTGTGATAAG CCGTAAAAAC ATGGTTTCCA 
    TCAGCGACTA TGGTGACTTC TATAAGATGG CGAAGCGTAA TATTATGCTC 
    GCAATTTTAG GTTTTAATGC GCAGAAACAC TTCTGCGATA CAAGAGAAAG 
    GATGGTCAGT AACGTGTTAA GCAGTTTGCA TAAATTGGTT GCTGTTGACC 
    CACATTCCCC TCTGTACTTC AGAGAAGTTT ACACTACTGA GCTATTCGGA 
    TTGTCCTTGA TCCAGAATTT AGGTGAGGAT GTGTGTTCAG TTTATGTGGA 
    AGAGTTTGGG AGGGAGATAT CCAAGGAAGA GATCTTCCAT GTCCTTGTGC 
    ATGAGATATT GTCGTGTGTC GTGGAGCCTG ATTGGAGGGA CTACTTCCCC 
    TACCTCAGCT GGCTTCCAAA CAAGAGCTTC GAAACAATAG TGTCTAGTAC 
    AGAATTTAGA CGAGATGCTG TGATGAACGC GTTGATCAAG AGGCAGAAGG 
    AAAGGATTGC GCGCGGAGAG GCAAGGATAT CCTATATTGA CTTCTTGCTG 
    GAAGCTAAGA ACAGTACACA GCTGACAGAT CACCAACTGA TGCTGCTGCT 
    GGCGGAGTCC ATCGCTGCTG CTGTGGATAC TGTCCTGGTA ACCACTGAAT 
    GGGCCATGTA TGAGCTTGCC AAGAACCCTG ACAAACAGGA ATGGCTTTAC 
    CGGGAGATTC GAGAGGTGTG CGGCGGCAAG GCGGTGACCG AGGAGGACCT 
    ACCAAGGCTG CCGTACCTCG ACGCCGTGCT CCACGAGACG CTGCGGCTGC 
    ACTCCCCGGT GCCGGTGCTC CCCACGAGGT TCGTCCACGA CGACACCACG 
    CTCGCCGGCT ACGACGTCCC CGCGGGCACC CAGGTGATGA TCAACGTGTT 
    CGGGTGCCAC ATGGACGAGG AGGCCTGGGA GTCGCCCGGA GAGTGGTCGC 
    CGGAGAGATT CCTCGGCGAG GGGTTCAAGT TGGCCGACAG GTACAAGACG 
    CTGGCGTTCG GCGCCGGGAG GAGGACCTGC GCGGGGAGCC AGCAGGCGGT 
    GAGCATCGCG TGCGTCGCCA TCGCGCGCTT CGTGCAGGAG CTCCAGTGGA 
    CGCTGAGGGA GGGCGACGGT GATAAGGAGG ATACCATGCA GTACACTGCC 
    TTGAAGCTTC ACCCGCTGCA CGTGCACCTC AAGCCCAGAG GAAGCTAGGA 
    TCGATCGATG TGAGCCAGAA GAAGCTAGCG GTTCCATGAT GAAGGCTAAA 
    TAATGATCAT ACCATCTCTG TTTTCTTAAT TAATATATTA GAAAAAATGT 
    CTTGCATTGT AACGGGTGCT ATGGCAACTA CTATTAGAAG ATGTGCAATA 
    AATTGAAACA TTGAAACCGT TATACGTTAT AATTGAAAAA GTTGATTAAA 
    ATC

    Gene models with high sequence identity

    1. AT5G25900.1 [+] show detail - GA requiring 3
      E-value: 3e-133; Score: 472.00

     

    InterPro Scan Digest

    Program Description E-value
    PatternScan Cytochrome P450, conserved site 0
    FPrintScan Cytochrome P450, E-class, group I 3.7e-29
    FPrintScan Cytochrome P450, E-class, group I 3.7e-29
    FPrintScan Cytochrome P450, E-class, group I 3.7e-29
    FPrintScan Cytochrome P450, E-class, group I 3.7e-29
    FPrintScan Cytochrome P450, E-class, group I 3.7e-29
    FPrintScan Cytochrome P450, E-class, group I 3.7e-29
    FPrintScan Cytochrome P450, E-class, group I 3.7e-29
    FPrintScan Cytochrome P450, C-terminal 0.00000027
    FPrintScan Cytochrome P450, C-terminal 0.00000027
    FPrintScan Cytochrome P450, C-terminal 0.00000027
    HMMPanther gb def: Ent-kaurene oxidase (EC 1.14.13.78) ( 1.6e-165
    HMMPanther Cytochrome P450 1.6e-165
    HMMPfam Cytochrome P450 1.1e-80
    superfamily Cytochrome P450 2.5e-105
    Gene3D Cytochrome P450 1.3e-93

    See the detailed result >

    AT5G25900.1

    Model type
    Protein coding
    Short Description
    GA requiring 3
    Curator Summary
    Encodes a member of the CYP701A cytochrome p450 family that is involved in later steps of the gibberellin biosynthetic pathway.
    Computational Description
    GA requiring 3 (GA3); FUNCTIONS IN: oxygen binding; INVOLVED IN: gibberellin biosynthetic process, gibberellic acid mediated signaling pathway, ent-kaurene oxidation to kaurenoic acid; LOCATED IN: chloroplast outer membrane, endoplasmic reticulum, microsome; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Cytochrome P450 (InterPro:IPR001128), Cytochrome P450, E-class, group I (InterPro:IPR002401), Cytochrome P450, conserved site (InterPro:IPR017972); BEST Arabidopsis thaliana protein match is: Cytochrome P450 superfamily protein (TAIR:AT5G07990.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR