K27638

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K27638

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage
Phenotypes
T1  
whole plant (PO:0000003)
  • increased height (PATO:0000570)
    (Adult plant::Plant height::tall)
cauline leaf (PO:0000013)
  • dark green (PATO:0001249)
    (Cauline leaf::Color::dark)
rosette leaf (PO:0000014)
  • increased size (PATO:0000586)
    (Rosette leaf::Shape::large)
  • dark green (PATO:0001249)
    (Rosette leaf::Color::dark)
root (PO:0009005)
  • present in greater numbers in organism (PATO:0000470)
    (Root::Number::many)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K27638

Determination of introduced cDNA(s)

  • J023111A19 - Rice Full-length cDNAs - Original: J023111A19

    full reading [+] show sequence

    Nucleotide sequence: J023111A19 - full reading
    GATACTTAGT ACTGTACGTA CTAAACCGGG GATTGATCAG GAGAGTGCAG 
    TGCAAACTCG CAGTGAACCT GTGTGATCGA TCGGCCATGG AGTCGCTGCT
    CGCAGCCGGT GCGGGAGGGA TCGGCGTGGC TGCGGCGGCC GCCGTCGTGG
    CGGCGACCCT CGCCGTGGTG CCGCCCAAGG ACCGCGGCAA CAACCCGCCT
    CCAGCTGTTC CTGGTTTACC CGTTATTGGA AATATGCATC AATTGAAAGA
    AAAGAAGCCT CATCATACCT TTACAAAATG GTCTAAAACT TATGGGCCAA
    TCTACACTAT AAAGACCGGA GCTTCTTCAG TAGTTGTGCT CAATTCAACG
    GAAGTAGCCA AGGAGGCGAT GATTGAAAAA TTCTCATCAA TATCAACTAA
    AAAGCTACCA AAAGCATTGT CTGTGATAAG CCGTAAAAAC ATGGTTTCCA
    TCAGCGACTA TGGTGACTTC TATAAGATGG CGAAGCGTAA TATTATGCTC
    GCAATTTTAG GTTTTAATGC GCAGAAACAC TTCTGCGATA CAAGAGAAAG
    GATGGTCAGT AACGTGTTAA GCAGTTTGCA TAAATTGGTT GCTGTTGACC
    CACATTCCCC TCTGTACTTC AGAGAAGTTT ACACTACTGA GCTATTCGGA
    TTGTCCTTGA TCCAGAATTT AGGTGAGGAT GTGTGTTCAG TTTATGTGGA
    AGAGTTTGGG AGGGAGATAT CCAAGGAAGA GATCTTCCAT GTCCTTGTGC
    ATGAGATATT GTCGTGTGTC GTGGAGCCTG ATTGGAGGGA CTACTTCCCC
    TACCTCAGCT GGCTTCCAAA CAAGAGCTTC GAAACAATAG TGTCTAGTAC
    AGAATTTAGA CGAGATGCTG TGATGAACGC GTTGATCAAG AGGCAGAAGG
    AAAGGATTGC GCGCGGAGAG GCAAGGATAT CCTATATTGA CTTCTTGCTG
    GAAGCTAAGA ACAGTACACA GCTGACAGAT CACCAACTGA TGCTGCTGCT
    GGCGGAGTCC ATCGCTGCTG CTGTGGATAC TGTCCTGGTA ACCACTGAAT
    GGGCCATGTA TGAGCTTGCC AAGAACCCTG ACAAACAGGA ATGGCTTTAC
    CGGGAGATTC GAGAGGTGTG CGGCGGCAAG GCGGTGACCG AGGAGGACCT
    ACCAAGGCTG CCGTACCTCG ACGCCGTGCT CCACGAGACG CTGCGGCTGC
    ACTCCCCGGT GCCGGTGCTC CCCACGAGGT TCGTCCACGA CGACACCACG
    CTCGCCGGCT ACGACGTCCC CGCGGGCACC CAGGTGATGA TCAACGTGTT
    CGGGTGCCAC ATGGACGAGG AGGCCTGGGA GTCGCCCGGA GAGTGGTCGC
    CGGAGAGATT CCTCGGCGAG GGGTTCAAGT TGGCCGACAG GTACAAGACG
    CTGGCGTTCG GCGCCGGGAG GAGGACCTGC GCGGGGAGCC AGCAGGCGGT
    GAGCATCGCG TGCGTCGCCA TCGCGCGCTT CGTGCAGGAG CTCCAGTGGA
    CGCTGAGGGA GGGCGACGGT GATAAGGAGG ATACCATGCA GTACACTGCC
    TTGAAGCTTC ACCCGCTGCA CGTGCACCTC AAGCCCAGAG GAAGCTAGGA
    TCGATCGATG TGAGCCAGAA GAAGCTAGCG GTTCCATGAT GAAGGCTAAA
    TAATGATCAT ACCATCTCTG TTTTCTTAAT TAATATATTA GAAAAAATGT
    CTTGCATTGT AACGGGTGCT ATGGCAACTA CTATTAGAAG ATGTGCAATA
    AATTGAAACA TTGAAACCGT TATACGTTAT AATTGAAAAA GTTGATTAAA
    ATC

    Gene models with high sequence identity

    1. AT5G25900.1 [+] show detail - GA requiring 3
      E-value: 3e-133; Score: 472.00

     

    InterPro Scan Digest

    Program Description E-value
    PatternScan Cytochrome P450, conserved site 0
    FPrintScan Cytochrome P450, E-class, group I 3.7e-29
    FPrintScan Cytochrome P450, E-class, group I 3.7e-29
    FPrintScan Cytochrome P450, E-class, group I 3.7e-29
    FPrintScan Cytochrome P450, E-class, group I 3.7e-29
    FPrintScan Cytochrome P450, E-class, group I 3.7e-29
    FPrintScan Cytochrome P450, E-class, group I 3.7e-29
    FPrintScan Cytochrome P450, E-class, group I 3.7e-29
    FPrintScan Cytochrome P450, C-terminal 2.7e-07
    FPrintScan Cytochrome P450, C-terminal 2.7e-07
    FPrintScan Cytochrome P450, C-terminal 2.7e-07
    HMMPanther gb def: Ent-kaurene oxidase (EC 1.14.13.78) ( 1.6e-165
    HMMPanther Cytochrome P450 1.6e-165
    HMMPfam Cytochrome P450 1.1e-80
    superfamily Cytochrome P450 2.5e-105
    Gene3D Cytochrome P450 1.3e-93

    See the detailed result >

    AT5G25900.1

    Model type
    Protein coding
    Short Description
    GA requiring 3
    Curator Summary
    Encodes a member of the CYP701A cytochrome p450 family that is involved in later steps of the gibberellin biosynthetic pathway.
    Computational Description
    GA requiring 3 (GA3); FUNCTIONS IN: oxygen binding; INVOLVED IN: gibberellin biosynthetic process, gibberellic acid mediated signaling pathway, ent-kaurene oxidation to kaurenoic acid; LOCATED IN: chloroplast outer membrane, endoplasmic reticulum, microsome; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Cytochrome P450 (InterPro:IPR001128), Cytochrome P450, E-class, group I (InterPro:IPR002401), Cytochrome P450, conserved site (InterPro:IPR017972); BEST Arabidopsis thaliana protein match is: Cytochrome P450 superfamily protein (TAIR:AT5G07990.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR