K27648

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K27648

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T1  
cauline leaf (PO:0000013)
  • present in fewer numbers in organism (PATO:0001997)
    (Cauline leaf::Number::few)
rosette leaf (PO:0000014)
  • decreased size (PATO:0000587)
    (Rosette leaf::Shape::small)
  • present in fewer numbers in organism (PATO:0001997)
    (Rosette leaf::Number::few)
  • abnormal (PATO:0000460)
    (Rosette leaf::Others)
seed (PO:0009010)
  • present in fewer numbers in organism (PATO:0001997)
    (Seed::Number::few)
stem (PO:0009047)
  • abnormal (PATO:0000460)
    (Stem::Others)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K27648

Determination of introduced cDNA(s)

  • J013118J02 - Rice Full-length cDNAs - Original: J013118J02

    full reading [+] show sequence

    Nucleotide sequence: J013118J02 - full reading 
    GGGTTCCCCT TCCTCTCTCA TCAGCACACA CTGTCACCAC AACAGCCACC 
    AACTCCTCTC AACTTCTTGA TCCGGCGGCG GCGGCAAGAT GTTCGGCGGC 
    GGCGGCGGCG GCGGGAGGAA GCCGCTGGAC TACGAGGAGC TGAACGAGAA 
    CGTGAAGAAG GTGCAGTACG CGGTGCGGGG GGAGCTGTAC CTGCGCGCCT 
    CCGAGCTCCA GAAGGAGGGC AAGAAGATCA TCTTCACCAA CGTCGGCAAC 
    CCACACGCGC TCGGCCAGAA GCCGCTCACC TTCCCCCGCC AGGTTGTGGC 
    GCTGTGCCAG GCCCCCTTCC TGCTCGATGA TCCCAACGTC GGCCTTATCT 
    TCCCCGCCGA CGCCATCGCG CGGGCCAAGC ACTACCTCGC CATGGCACCC 
    GGTGGACTAG GTGCTTACAG TGATTCCCGA GGTATCCCTG GTATTAGGAA 
    GGAAGTCGCC GAGTTCATCG AGAGGCGTGA TGGTTATCCA AGTGATCCAG 
    AACTTATTTA CCTCACAGAT GGTGCCAGCA AAGGTGTGAT GCAAATGCTG 
    AATACCATTA TCAGAAATGA GAGAGATGGG ATTCTGGTTC CTGTTCCACA 
    ATACCCGCTT TATTCTGCTG CCATTTCCCT CTTTGGTGGT TCTCTCGTGC 
    CATACTACTT AGAAGAAGAG GCTAACTGGG GACTTGACTT CGTCAATCTC 
    CGACAGACTG TGGCGTCAGC GCGGTCAAAG GGAATCACTG TTCGAGCAAT 
    GGTGATTATC AACCCAGGAA ACCCTACTGG CCAATGCCTT AGTGAAGGAA 
    ACATAAAGGA ACTTCTCAAA TTCTGCTTCC ATGAGAACTT AGTTCTGCTT 
    GCAGATGAAG TCTATCAACA GAACATTTAT CAAGATGAGC GCCCATTTAT 
    AAGTGCTAGA AAGGTTCTGT TTGACATGGG TCCTCCTATG AGCAGGGAAG 
    TTCAGCTGGT TTCTTTCCAT ACTGTGTCAA AAGGATATTG GGGGGAGTGT 
    GGACAACGTG GAGGGTATTT TGAAATGACA AATCTTCCTC CCAAGACAGT 
    AGACGAGATC TACAAGGTTG CATCAATCGC ACTCAGTCCA AATGTTCCTG 
    GGCAGATCTT TATGGGTTTA ATGGTTAACC CTCCTAAGCC TGGAGATATC 
    TCTTATCTGA AGTTTTCTGC TGAAAGCAAG TCTATCCTCG AGTCTTTGAG 
    GAGGAGAGCA CGCCTGATGA CAGATGGTTT CAATAGTTGC CGAAATGTTG 
    TCTGCAATTT CACAGAAGGA GCTATGTACT CTTTCCCCCA AATACGCTTA 
    CCACCAAAAG CTATAGATGC AGCCAAAAGG GCTGGCAAAG CGGCCGATGT 
    TTTCTACTGC CTCAAGCTTC TTGAAGCAAC TGGAATATCC ACTGTTCCAG 
    GGTCAGGTTT CGGACAAAAA GAAGGGGTGT TCCACCTGAG GACGACCATC 
    CTGCCAGCTG AGGAGGACAT GCCTGCCATC ATGACCAGCT TCAAGAAGTT 
    CAACGACACT TTCATGGATC AGTACGATGG CTACTCCAGG ATGTGAAATG 
    AAAACAAGAT GAGCACTACC CCAGGAGAAG AGAGAAAGAA TCTGCCTCAG 
    CGTGGCTGAT CGAGCAGTTA TAATTAAAAG GCACTGTTAA TTAATTAATT 
    AATCAATCAA TCGAAAGGCT AATTATTTGC TGCAACAATC TGTAACTCGA 
    TCTACTGAAG GAAAAAAAAA ACTTCTTGTC CTTGATATGC ATGATGTATA 
    GATAAAATTA TTTCTGCTCT TTGGGAATTG AATAATACGG GCGCACCCAC 
    CGGTTACAGT CTACTATACA TGGTCCTGTC TGTAAGGCTG CAGTAGCGAA 
    CTATTTCTAT GTTTTGATAC TGTGAACAAG TTGCTTGGCA AGTCACTTGT 
    GCTGGCTGAA ATATAATGCA GATCAATATA TGATCAGATC AATGCAGAAT 
    TATACATTGG TCTCTAAATT

    Gene models with high sequence identity

    1. AT1G23310.1 [+] show detail - glutamate:glyoxylate aminotransferase
      E-value: 0; Score: 813.00

     

    InterPro Scan Digest

    Program Description E-value
    superfamily Pyridoxal phosphate-dependent transferase, major domain 5.1e-68
    FPrintScan 1-aminocyclopropane-1-carboxylate synthase 0.000011
    FPrintScan 1-aminocyclopropane-1-carboxylate synthase 0.000011
    FPrintScan 1-aminocyclopropane-1-carboxylate synthase 0.000011
    Gene3D Pyridoxal phosphate-dependent transferase, major region, subdomain 1 1.8e-44
    Gene3D Pyridoxal phosphate-dependent transferase, major region, subdomain 2 0.000000000000011
    HMMPanther ALANINE AMINOTRANSFERASE 1.6e-218
    HMMPanther SUBGROUP I AMINOTRANSFERASE RELATED 1.6e-218
    HMMPfam Aminotransferase, class I/II 1.9e-34

    See the detailed result >

    AT1G23310.1

    Model type
    Protein coding
    Short Description
    glutamate:glyoxylate aminotransferase
    Curator Summary
    Identified by cloning the gene that corresponded to a purified protein having glyoxylate aminotransferase activity. Localized to the peroxisome and thought to be involved in photorespiration/ metabolic salvage pathway.
    Computational Description
    glutamate:glyoxylate aminotransferase (GGT1); FUNCTIONS IN: glycine:2-oxoglutarate aminotransferase activity, L-alanine:2-oxoglutarate aminotransferase activity, alanine-glyoxylate transaminase activity; INVOLVED IN: photorespiration; LOCATED IN: apoplast, peroxisome, chloroplast, vacuole, membrane; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 14 growth stages; CONTAINS InterPro DOMAIN/s: 1-aminocyclopropane-1-carboxylate synthase (InterPro:IPR001176), Aminotransferase, class I/classII (InterPro:IPR004839), Pyridoxal phosphate-dependent transferase, major domain (InterPro:IPR015424), Pyridoxal phosphate-dependent transferase, major region, subdomain 1 (InterPro:IPR015421), Pyridoxal phosphate-dependent transferase, major region, subdomain 2 (InterPro:IPR015422); BEST Arabidopsis thaliana protein match is: alanine-2-oxoglutarate aminotransferase 2 (TAIR:AT1G70580.4); Has 27976 Blast hits to 27968 proteins in 2949 species: Archae - 777; Bacteria - 19095; Metazoa - 617; Fungi - 736; Plants - 1276; Viruses - 0; Other Eukaryotes - 5475 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR