K27733

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K27733

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T2 seed (PO:0009010)
  • decreased width (PATO:0000599)
    (Seed::Shape::narrow)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K27733

Determination of introduced cDNA(s)

  • J033149M03 - Rice Full-length cDNAs - Original: J033149M03

    full reading [+] show sequence

    Nucleotide sequence: J033149M03 - full reading 
    GGGATCTGGA TCTGATCCAA CTCCCACCCA ACTCTCCAAG TACGGCAGCA 
    GCACTGGCTA CTGCGGAGGA GGCGTTGCGA CTTGCGAGTG GCGGAGGACT 
    AGGAAGTCGC CTCCGCCTCC GCTGCAGACA CACTGGCGAT GGCTTCCTCC 
    TTCGCCGCCG TCTTCTTCAG CAGCGCCTTC GCTGCCTGCT TCGCTGAGGT 
    GTGCACCATT CCTCTCGACA CAGCCAAAGT GCGGCTTCAG CTGCAAAAGA 
    AAGCAGCCCT AGCTACAGGC GGAGGAGGAG GGACCACTGG AGGAATGCTG 
    GGCACAATAA TGTGCATCGC AAGGGAGGAA GGGGTGGCCG CGCTTTGGAA 
    CGGCATCATC CCTGGCCTTC ACCGCCAGTG CGTCTATGGC GGACTCCGCA 
    TCGCCTTGTA TGAGCCTGTG AAAGCCTTCT TTATCCGAGA CGGAGATACT 
    GTTGCTGGTG GTGTCAGTTT ATTTGCTAAG ATCCTTGCTG CTCTTATGAC 
    AGCCAACCAG TTTACATCCA AATTTCATGT GTTAAGGACT TAAGGTAGGT 
    GCACTATGCA CAAGTAAATA TACTCTTATA TTAATTACCG TTCTATATAT 
    TCATAGGTGT CATAGCAATT GTTGTGGCAA ATCCAACTGA TCTTGTCAAA 
    GTGAGATTGC AAGCTGATGG AAAGGCCACC ACTGTCAAGA GGCACTATTC 
    TGGAGCCCTC AATGCGTATG CTACCATAAT CAGACAGGAA GGTATTGGAG 
    CTTTGTGGAC TGGCCTCGGT CCAAACATAG CACGCAATGC TATAATTAAT 
    GCTACCGAGT TGGCCAGCTA CGACCAACTC AAACAGCTGG ACTTGGTGCT 
    GGATTTTTTG CTGTTTGCAT TGGCTCTCCA GTGGATGTGG TGAAATCAAG 
    AATGATGGGT GACGCAACGT ACAAGAGTAC ACTAGACTGT TTTGCAAAGA 
    CATTAAAGAA TGATGGACTT CCTGCTTTTT ATAAAGGGTT CATTGCAAAC 
    TTTTGCCGCA TTGGGTCATG GAATGTGATA ATGTTTTTAA CACTGGAGCA 
    GGTAAGAAGA TCAATTCTGT AAGTCTGCTG CACTGCACTG CAAGCCAAGG 
    ATCTATCATG CAAGTGAACA GCGGGAGCAG GACTGAATCG ATGCTTGATT 
    TAGCTGTAGC TCCTGAGTCC ACTTTATAGT GCTGTTTGCT GAAGGCCACA 
    TATGTCTAGG ATTAGATTTG TTTTGTACAG GACAATGCGT TCTAATTAAA 
    CCCATCAATT CAGCAGAGGA CAACATCAAC AGAGTCATTT GTGCAGAGAT 
    GATTGGCCAT AACATGTCAT TGTACTGTCT GAGCAATTTT TTAATTTTAC 
    CAAAAATGAA ACTCACAAAA TCTAATGCAA ATATAGGTTA CTCTTGGTAG 
    TAATTTCATG TATCCGAGGT TTGTAAACAA TATGAAAGGA AGTTGTGACT 
    T

    Gene models with high sequence identity

    1. AT3G54110.1 [+] show detail - plant uncoupling mitochondrial protein 1
      E-value: 1e-81; Score: 106.00

     

    InterPro Scan Digest

    Program Description E-value
    HMMPanther MITOCHONDRIAL UNCOUPLING PROTEIN 2.9e-44
    HMMPanther Mitochondrial substrate carrier 2.9e-44
    HMMPanther MITOCHONDRIAL UNCOUPLING PROTEIN 2e-54
    HMMPanther Mitochondrial substrate carrier 2e-54
    HMMPanther MITOCHONDRIAL UNCOUPLING PROTEIN 6.4e-52
    HMMPanther Mitochondrial substrate carrier 6.4e-52
    ProfileScan Mitochondrial substrate/solute carrier 21.784
    ProfileScan Mitochondrial substrate/solute carrier 25.002
    ProfileScan Mitochondrial substrate/solute carrier 24.594
    superfamily Mitochondrial substrate carrier 2.8e-20
    superfamily Mitochondrial substrate carrier 2.1e-20
    superfamily Mitochondrial substrate carrier 7e-19
    HMMPfam Mitochondrial substrate/solute carrier 2.1e-18
    HMMPfam Mitochondrial substrate/solute carrier 2.1e-20
    HMMPfam Mitochondrial substrate/solute carrier 6e-20

    See the detailed result >

    AT3G54110.1

    Model type
    Protein coding
    Short Description
    plant uncoupling mitochondrial protein 1
    Curator Summary
    Member of Uncoupling protein PUMP2 family. Encodes a mitochondrial uncoupling protein AtUCP1 involved in maintain the redox poise of the mitochondrial electron transport chain to facilitate photosynthetic metabolism. Disruption of UCP1 results in a photosynthetic phenotype. Specifically there is a restriction in photorespiration with a decrease in the rate of oxidation of photorespiratory glycine in the mitochondrion. This change leads to an associated reduced photosynthetic carbon assimilation rate.
    Computational Description
    plant uncoupling mitochondrial protein 1 (PUMP1); FUNCTIONS IN: oxidative phosphorylation uncoupler activity, binding; INVOLVED IN: transport, photosynthesis, photorespiration; LOCATED IN: mitochondrion, mitochondrial inner membrane, plasma membrane, chloroplast; EXPRESSED IN: 26 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Mitochondrial substrate carrier (InterPro:IPR001993), Mitochondrial brown fat uncoupling protein (InterPro:IPR002030), Mitochondrial substrate/solute carrier (InterPro:IPR018108); BEST Arabidopsis thaliana protein match is: uncoupling protein 2 (TAIR:AT5G58970.1); Has 26567 Blast hits to 13376 proteins in 459 species: Archae - 0; Bacteria - 2; Metazoa - 11203; Fungi - 7876; Plants - 4909; Viruses - 3; Other Eukaryotes - 2574 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR