K27744

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K27744

Phenotypes

Morphological phenotypes

Specific phenotypess were NOT observed.

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K27744

Determination of introduced cDNA(s)

  • J013163D13 - Rice Full-length cDNAs - Original: J013163D13

    full reading [+] show sequence

    Nucleotide sequence: J013163D13 - full reading 
    GATCCGTCCT CCTCCTCCTC CTCTCTTCCA TCGCCGCTGC TCGCTTCCTC 
    CCCCTCTCCC ACGAAAGATG GCGTCTCACA TTGTTGGGTA CCCCCGCATG 
    GGCCCCAAGA GGGAGCTCAA GTTTGCCCTG GAGTCTTTCT GGGATGGGAA 
    GAGCAGCGCT GAGGATTTGG AGAAGGTTGC GACCGACCTC AGGGCCAGCA 
    TCTGGAAGCA AATGGCTGAC GCTGGGATCA AGTACATCCC CAGCAACACC 
    TTCTCGTACT ATGATCAGGT TCTTGACACG GCTGCCATGC TTGGTGCTGT 
    CCCAGAGCGC TACTCATGGA CTGGTGGAGA GATTGGGTTC AGCACCTACT 
    TCTCGATGGC TAGGGGCAAT GCCACTGTCC CTGCCATGGA GATGACCAAG 
    TGGTTTGACA CCAACTACCA CTTTATCGTC CCTGAACTTG GTCCCAACAC 
    CAAGTTCTCC TACTCTTCCC ACAAGGCTGT GAATGAATAC AAGGAGGCTA 
    AGGCGCTCGG CGTTGACACC GTGCCAGTAC TTGTTGGACC AGTTTCATAC 
    TTGTTGCTCT CCAAACCTGC CAAGGGTGTA GAGAAGTCGT TCGCTCTTCT 
    TTCCCTTCTG AGCAGCATCC TTCCTGTCTA CAAGGAGGTT ATTGCTGAGT 
    TGAAGGCAGC TGGTGCTACT TGGATTCAGT TTGATGAACC CACACTTGTT 
    CTTGACCTTG ACTCTCACCA ATTGGCTGCA TTCTCTGCTG CATACACAGA 
    ACTTGAGTCG GCACTTTCTG GATTGAATGT GCTTATTGAG ACATACTTCG 
    CTGACATTCC TGCTGAATCA TACAAGACCC TCACATCCCT GAACAGCGTG 
    ACTGCTTATG GTTTTGACCT TATCCGTGGA GCCAAGACTC TTGACCTTAT 
    CAAGAGTGCT GGTTTCCCCT CTGGAAAGTA CCTCTTTGCC GGTGTTGTGG 
    ATGGACGCAA CATCTGGGCT GATGATCTCG CTGCATCTCT CACTACCCTT 
    GAGTCTCTTG AGGCTATTGT TGGAAAGGAC AAGCTTGTGG TCTCGACTTC 
    CTGCTCACTC ATGCACACCG CTGTGGATCT TGTAAATGAG ACCAAGCTCG 
    ATAGCGAGAT TAAGTCATGG CTCGCTTTTG CTGCCCAAAA GGTGGTTGAG 
    GTGAATGCCC TTGCTAAGGC ATTGGCTGGA CAGAAGGATG AGGCTTACTT 
    TGCAGCAAAC GCTGCTGCTC AGGCCTCCAG GAGGTCATCA CCTCGTGTGA 
    CAAACGAGGA AGTCCAAAAG GCTGCTGCTG CTCTGAAGGG GTCTGACCAC 
    CGCCGTGCTA CCAATGTTAG CGCTAGACTG GACGCACAGC AGAAGAAGCT 
    CAACCTTCCA GTCCTTCCTA CAACCACAAT TGGTTCATTC CCACAGACTG 
    TGGAGCTCAG GAGAGTCCGC CGTGAGTACA AGGCAAAAAA GATCTCTGAG 
    GAGGAGTACA TCAGTGCCAT CAAGGAGGAA ATCAGCAAGG TTGTTAAGAT 
    CCAGGAAGAG CTTGACATCG ATGTCCTTGT TCACGGCGAG CCTGAGAGAA 
    ACGATATGGT TGAGTACTTC GGTGAGCAGC TTTCTGGTTT TGCATTCACT 
    GCCAACGGAT GGGTGCAATC TTATGGATCA CGGTGTGTCA AGCCACCAAT 
    TATCTATGGT GATGTGAGCC GCCCCAACCC CATGACTGTG TTCTGGTCCA 
    AGTTGGCGCA GAGCATGACC TCTCGCCCCA TGAAGGGAAT GTTGACTGGT 
    CCCGTCACAA TCCTTAACTG GTCTTTTGTC AGGAATGACC AGCCGAGGTT 
    TGAGACATGC TACCAGATTG CTCTTGCCAT CAAGAAGGAG GTCGAGGACC 
    TTGAGGCTGG TGGTATTCAG GTCATCCAAA TCGATGAGGC TGCTTTGAGA 
    GAAGGTCTGC CACTCCGCAA GGCGGAGCAT GCTTTCTACT TGGACTGGGC 
    TGTTCACTCC TTCAGAATCA CCAACTGCGG TGTTCAGGAC ACCACCCAGA 
    TCCACACCCA CATGTGCTAC TCTAACTTCA ACGACATCAT CCACTCCATC 
    ATCAACATGG ACGCTGATGT GATCACCATC GAGAACTCCC GGTCCGACGA 
    GAAGCTGCTC TCCGTCTTCC GCGAGGGTGT GAAGTATGGT GCTGGCATTG 
    GCCCTGGTGT CTATGACATC CACTCCCCAA GGATCCCGTC CACAGAGGAG 
    ATTGCGGACC GCGTCAACAA GATGCTCGCG GTGCTCGACA CTAACATTCT 
    CTGGGTGAAC CCTGACTGCG GTCTCAAGAC CCGCAAGTAC AACGAGGTCA 
    AGCCCGCCCT TACCAACATG GTTTCGGCCG CCAAGCTCAT CCGCACCCAG 
    CTCGCCAGCG CGAAGTGAGG ATATCCATAG CTGCAAGTTC CCTTCGGTTG 
    CGAGGACGAT GCCGTCATAG CCATCTGTTT TTGAATAATC TGGGTCCAGT 
    GGATCCAATT GTCTGTGGTT AGGTTTAGTC AGTCTCGGTG GGAAGATCCC 
    TCATACCCGT TTTACTTTTG AATCAATTTG GTGGTTTTTG AGGCAGTTTG 
    TCGGTGTATT TGTATTGTGT GTAATCTGAG TGGGAATCAG CAGTGTTTCA 
    TCTGCATCTG AATCGTTGCG GATCAAATTA CAAAATTTTC

    Gene models with high sequence identity

    1. AT5G17920.2 [+] show detail - Cobalamin-independent synthase family protein
      E-value: 0; Score: 999.99

     

    InterPro Scan Digest

    Program Description E-value
    HMMTigr 5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase 0
    HMMPfam Methionine synthase, vitamin-B12 independent 1.3e-148
    HMMPfam Cobalamin (vitamin B12)-independent methionine synthase MetE, N-terminal 1.9e-117
    superfamily UROD/MetE-like 3.4e-129
    superfamily UROD/MetE-like 2.6e-125
    HMMPIR 5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase 0
    HAMAP 5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase 36.046
    Gene3D no description 4.7e-66
    Gene3D no description 3.4e-104

    See the detailed result >

    AT5G17920.2

    Model type
    Protein coding
    Short Description
    Cobalamin-independent synthase family protein
    Curator Summary
    Encodes a cytosolic cobalamin-independent methionine synthase, involved in methionine regeneration via the activated methyl cycle (SAM cycle). The protein undergoes thiolation following treatment with the oxidant tert-butylhydroperoxide.
    Computational Description
    COBALAMIN-INDEPENDENT METHIONINE SYNTHASE (ATCIMS); FUNCTIONS IN: 5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity, methionine synthase activity; INVOLVED IN: response to zinc ion, response to salt stress, methionine biosynthetic process; LOCATED IN: in 6 components; EXPRESSED IN: 9 plant structures; EXPRESSED DURING: seedling growth, seed development stages; CONTAINS InterPro DOMAIN/s: Cobalamin (vitamin B12)-independent methionine synthase MetE, N-terminal (InterPro:IPR013215), Methionine synthase, vitamin-B12 independent (InterPro:IPR002629), 5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase (InterPro:IPR006276); BEST Arabidopsis thaliana protein match is: methionine synthase 2 (TAIR:AT3G03780.3); Has 4442 Blast hits to 4434 proteins in 1682 species: Archae - 183; Bacteria - 3246; Metazoa - 14; Fungi - 206; Plants - 251; Viruses - 2; Other Eukaryotes - 540 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR