K28114

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K28114

Phenotypes

Morphological phenotypes

Specific phenotypess were NOT observed.

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K28114

Determination of introduced cDNA(s)

  • J033061O03 - Rice Full-length cDNAs - Original: J033061O03

    full reading [+] show sequence

    Nucleotide sequence: J033061O03 - full reading 
    GAATCATCCA TCTCCGAAGT GTGTCTGCAG CATGCAGGTG CTGAACACCA 
    TGGTGAACAA ACACTTCTTG TCCCTTTCGG TCCTCATCGT CCTCCTTGGC 
    CTCTCCTCCA ACTTGACAGC CGGGCAAGTC CTGTTTCAGG GATTCAACTG 
    GGAGTCGTGG AAGGAGAATG GCGGGTGGTA CAACTTCCTG ATGGGCAAGG 
    TGGACGACAT CGCCGCAGCC GGCATCACCC ACGTCTGGCT CCCTCCGCCG 
    TCTCACTCTG TCGGCGAGCA AGGCTACATG CCTGGGCGGC TGTACGATCT 
    GGACGCGTCT AAGTACGGCA ACGAGGCGCA GCTCAAGTCG CTGATCGAGG 
    CGTTCCATGG CAAGGGCGTC CAGGTGATCG CCGACATCGT CATCAACCAC 
    CGCACGGCGG AGCACAAGGA CGGCCGCGGC ATCTACTGCC TCTTCGAGGG 
    CGGGACGCCC GACTCCCGCC TCGACTGGGG CCCGCACATG ATCTGCCGCG 
    ACGACCCCTA CGGCGATGGC ACCGGCAACC CGGACACCGG CGCCGACTTC 
    GCCGCCGCGC CGGACATCGA CCACCTCAAC AAGCGCGTCC AGCGGGAGCT 
    CATTGGCTGG CTCGACTGGC TCAAGATGGA CATCGGCTTC GACGCGTGGC 
    GCCTCGACTT CGCCAAGGGC TACTCCGCCG ACATGGCAAA GATCTACATC 
    GACGCCACCG AGCCGAGCTT CGCCGTGGCC GAGATATGGA CGTCCATGGC 
    GAACGGCGGG GACGGCAAGC CGAACTACGA CCAGAACGCG CACCGGCAGG 
    AGCTGGTCAA CTGGGTCGAT CGTGTCGGCG GCGCCAACAG CAACGCCACG 
    GCGTTCGACT TCACCACCAA GGGCATCCTC AACGTCGCCG TGGAGGGCGA 
    GCTGTGGCGC CTCCGCGGCG AGGACGGCAA GGCGCCCGGC ATGATCGGGT 
    GGTGGCCGGC CAAGGCGACG ACCTTCGTCG ACAACCACGA CACCGGCTCG 
    ACGCAGCACC TGTGGCCGTT CCCCTCCGAC AAGGTCATGC AGGGCTACGC 
    ATACATCCTC ACCCACCCCG GCAACCCATG CATCTTCTAC GACCATTTCT 
    TCGATTGGGG TCTCAAGGAG GAGATCGAGC GCCTGGTGTC AATCAGAAAC 
    CGGCAGGGGA TCCACCCGGC GAGCGAGCTG CGCATCATGG AAGCTGACAG 
    CGATCTCTAC CTCGCGGAGA TCGATGGCAA GGTGATCACA AAGATTGGAC 
    CAAGATACGA CGTCGAACAC CTCATCCCCG AAGGCTTCCA GGTCGTCGCG 
    CACGGTGATG GCTACGCAAT CTGGGAGAAA ATCTGAGCGC ACGATGACGA 
    GACTCTCAGT TTAGCAGATT TAACCTGCGA TTTTTACCCT GACCGGTATA 
    CGTATATACG TGCCGGCAAC GAGCTGTATC CGATCCGAAT TACGGATGCA 
    ATTGTCCACG AAGTACTTCC TCCGTAAATA AAGTGAGGAT CAGGGACATA 
    CATTTGTATG GTTTTACGAA TAATGCTATG CAATAAAATT TGCACTGCTT 
    AATGCTTATG C

    Gene models with high sequence identity

    1. AT4G25000.1 [+] show detail - alpha-amylase-like
      E-value: 1e-147; Score: 520.00

     

    InterPro Scan Digest

    Program Description E-value
    HMMSmart Glycosyl hydrolase, family 13, subfamily, catalytic domain 9.3e-56
    HMMSmart Alpha-amylase, C-terminal beta-sheet 4.8e-35
    HMMPIR Alpha-amylase, plant 0
    superfamily Glycoside hydrolase, catalytic core 9.8e-80
    HMMPfam Glycosyl hydrolase, family 13, catalytic domain 3e-24
    HMMPfam Alpha-amylase, C-terminal beta-sheet 1.9e-22
    FPrintScan Glycoside hydrolase family 13 8.4e-07
    FPrintScan Glycoside hydrolase family 13 8.4e-07
    FPrintScan Glycoside hydrolase family 13 8.4e-07
    HMMPanther ALPHA-AMYLASE 3.3e-198
    HMMPanther AMYLASE 3.3e-198
    Gene3D Glycoside hydrolase, subgroup, catalytic core 1.6e-103

    See the detailed result >

    AT4G25000.1

    Model type
    Protein coding
    Short Description
    alpha-amylase-like
    Curator Summary
    Predicted to be secreted protein based on signalP prediction. Involved in starch mobilization. Mutants are defective in alpha-amylase activity. (Note: AMY1 has been found in the literature to be referred to as AMY3, which is not to be confused with AMY3/At1g69830).
    Computational Description
    alpha-amylase-like (AMY1); FUNCTIONS IN: alpha-amylase activity; INVOLVED IN: response to gibberellin stimulus, response to abscisic acid stimulus; LOCATED IN: extracellular region, apoplast; EXPRESSED IN: 20 plant structures; EXPRESSED DURING: 11 growth stages; CONTAINS InterPro DOMAIN/s: Glycoside hydrolase family 13 (InterPro:IPR006046), Alpha-amylase, plant (InterPro:IPR013775), Glycoside hydrolase, catalytic core (InterPro:IPR017853), Glycoside hydrolase, subgroup, catalytic core (InterPro:IPR013781), Glycosyl hydrolase, family 13, catalytic domain (InterPro:IPR006047), Alpha-amylase, C-terminal beta-sheet (InterPro:IPR012850); BEST Arabidopsis thaliana protein match is: alpha-amylase-like 2 (TAIR:AT1G76130.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR