K29123

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K29123

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage
Phenotypes
T2 seed (PO:0009010)
  • decreased width (PATO:0000599)
    (Seed::Shape::narrow)
  • low saturation (PATO:0000328)
    (Seed::Color::pale)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K29123

Determination of introduced cDNA(s)

  • J013055H16 - Rice Full-length cDNAs - Original: J013055H16

    full reading [+] show sequence

    Nucleotide sequence: J013055H16 - full reading
    TATTGGGATA AGGCAACAGC AAAGGAACAT TTACTTCTTC CTCCCGAGTT 
    CCCCCAAACT CCTTCCTTCT TCCGATCTCC CACCGTCGCC TGGGCGCCTG
    GCGGTTTGCT CCACTCCATA GGCGGCCATC GTCTTCTCAA CCAGAAAGCT
    TCTGGCTGTA CAGCTTGGCG GGATACAAGA CGACGGTCTG CCGTCGGCGA
    TCCGCAGTCG CCGGAGCTGA AGGTGGAAGC GGCCAACACG GCAGTCCGTG
    CAGCCGCTTG AGAATTTTTT GTCATGGCAG TGAAAAGATG CGCAGAAGTG
    CCGATATCTG CTAGGCCGGG AAAGATGATG CGGATTGCTA TTAAGTCTTG
    GTCAAGGTTC TTGCCCCCTT ACATCCCTGA CGATGTGATC TTCGACATTC
    TGTTACGGCT GCCATCCAAA TCTCTTATCC GATTTAAGTC CGTGTGTAAG
    GCTTGGCACG CCATAATATC CAACCCCTGT TTCATCAGTG CTCACCTCGA
    GTGCTCTAAG CAGAAGCCAT CGATATTTAT GGTCCCTGGT GTTTACGAGA
    AGCAGAATAA TGGCGAGAAT ACTTCCTTCC TGATGGGCCT CTATCAGTAT
    CAGGGTGGTA ATATAATGGA ACAAATCCAT GTACAAGACT TTCCACAAGG
    CATTGGTACA TGGAGTCGTC CGATCCACTG CAATGGCATG CTCCTCATTT
    CCACCATGAA CCACGAAATG ATTGTCTGCA ACCCATCAAC TAGAGAGATT
    GTCTCCCTGC CAAAAGGGAG TTATAACCTT CATGCGGGCC CAAGGGCTGG
    ATTTGGGTTT GACCCTCACA GCAACAAGTA TAAGGTGGCT AGATTTTTCT
    ACCAGAGGGA CGATGACACA TCAGAATTGG TCTGCAAGTT TGAAGTGCTT
    ACTCTAGGCA CTAATTTGTG GAGGCAAACT GAGGATCCGC CATATCCAAT
    TAGTGGGTTA ACTCCTGTTC ATGTGAAAGG CGCCATCTAC TGGATGGTTA
    ATACGCCACT TTGCCCTGAT CCACCCAATG CATTTCTTAG GTTCTGTTTG
    ACTAACGAGA AGTTTAGTCT GCTTCAGTAT CCTCCATGTA ATCTAAAGCC
    TACCCGTTTC ATAGAAGTGG AGGGTGAACT ATGCTGTGCT TGCTTTTGCA
    GTCAGGTGAG CGCGTTGAAG ATCTGGACCT GCAATTATGC ACAGAACCCT
    GAATGGACTC AGCGCTGCAC TGTACAGATT CCACCTGATA TAGTTGTGAA
    TAATCCTGTA GCAAGGCCTC CTATAGTTTT TCTTCATGGA AAAAAGCTGC
    TACTGACATG GAATCAGGTC TACCAGTATG ACATTCAAAC CTGTAGAATG
    GAAAAGATAG CTTCAGGGGT TGAAGACTTC ACGTGCTATG ATCCAAGGAA
    CAATAAGTAC TGGGCATATC TGGAGAAAGA AGTAACGGAT ATGCATCTAT
    TTAATTATGC TGAGAGTCTA GTTCCGATTA GAGAATTTTG AGAAGTCACT
    TCATTTTTTG TTAAGATGTG GAGCCTTGCA TTTCAGGCCC AAAGAGTCCT
    CATGACCGTG TCCCTTTGAA GGAAATGAAA TCAGATTGGC ATGCTTGCCT
    GGACAGCAAT TTCAAGCAAT ATGCAGTTAC ACAAACACAT CAAATCCCAG
    CGTTATAATT GGTGCTGGCC TAGTCGCAAA GAAAGCCTGT GAATTGGGCC
    TTGAGGTTTG CATGTTCGTT CATATATGTA ACTAGTGTAT TGTGTCGTGT
    GTTTGTGCGA CTATAACCTC CCTCTCTGTA AATATGTATA ATAATTAACT
    TAATTTGTTA ATAACATTTA ATAATTTAAA TTAGTATTTC CTCCTTTTAT
    AAG

    Gene models with high sequence identity

    1. AT2G05710.1 [+] show detail - aconitase 3
      E-value: 2e-21; Score: 53.10

     

    InterPro Scan Digest

    Program Description E-value
    superfamily F-box domain, Skp2-like 1.7e-11
    HMMPanther Aconitase/Iron regulatory protein 2/2-methylisocitrate dehydratase 1e-11
    HMMPanther Aconitase-like core 1e-11
    HMMTigr F-box associated interaction domain 5.4e-19
    ProfileScan F-box domain, cyclin-like 8.941
    HMMPfam F-box domain, cyclin-like 8.5e-07
    HMMPfam F-box associated domain, type 1 2e-06
    HMMSmart F-box domain, cyclin-like 4.3e-08

    See the detailed result >

    AT2G05710.1

    Model type
    Protein coding
    Short Description
    aconitase 3
    Curator Summary
    Encodes an aconitase that can catalyze the conversion of citrate to isocitrate through a cis-aconitate intermediate, indicating that it may participate in the TCA cycle and other primary metabolic pathways. The protein is believed to accumulate in the mitochondria and the cytosol. It affects CSD2 (At2g28190 - a superoxide dismutase) transcript levels and may play a role in the response to oxidative stress. One member of the family (ACO1 - At35830) was shown to specifically bind to the 5' UTR of CSD2 in vitro. ACO3 is tyrosine-phosphorylated and its phosphorylation state is modulated in response to ABA in Arabidopsis thaliana seeds.
    Computational Description
    aconitase 3 (ACO3); FUNCTIONS IN: aconitate hydratase activity, copper ion binding, ATP binding; INVOLVED IN: in 6 processes; LOCATED IN: cytosol, mitochondrion, cell wall, plasma membrane, chloroplast; EXPRESSED IN: 27 plant structures; EXPRESSED DURING: 16 growth stages; CONTAINS InterPro DOMAIN/s: Aconitase/3-isopropylmalate dehydratase large subunit, alpha/beta/alpha (InterPro:IPR001030), Aconitase family, 4Fe-4S cluster binding site (InterPro:IPR018136), Aconitase A/isopropylmalate dehydratase small subunit, swivel (InterPro:IPR000573), Aconitase/3-isopropylmalate dehydratase large subunit, alpha/beta/alpha, subdomain 2 (InterPro:IPR015932), Aconitase/Iron regulatory protein 2/2-methylisocitrate dehydratase (InterPro:IPR015934), Aconitase-like core (InterPro:IPR015937), Aconitase/3-isopropylmalate dehydratase, swivel (InterPro:IPR015928), Aconitase/iron regulatory protein 2 (InterPro:IPR006249), Aconitase/3-isopropylmalate dehydratase large subunit, alpha/beta/alpha, subdomain 1/3 (InterPro:IPR015931); BEST Arabidopsis thaliana protein match is: aconitase 1 (TAIR:AT4G35830.1); Has 20552 Blast hits to 20369 proteins in 2575 species: Archae - 558; Bacteria - 10777; Metazoa - 503; Fungi - 660; Plants - 232; Viruses - 0; Other Eukaryotes - 7822 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR