K29123

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K29123

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T2 seed (PO:0009010)
  • decreased width (PATO:0000599)
    (Seed::Shape::narrow)
  • low saturation (PATO:0000328)
    (Seed::Color::pale)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K29123

Determination of introduced cDNA(s)

  • J013055H16 - Rice Full-length cDNAs - Original: J013055H16

    full reading [+] show sequence

    Nucleotide sequence: J013055H16 - full reading 
    TATTGGGATA AGGCAACAGC AAAGGAACAT TTACTTCTTC CTCCCGAGTT 
    CCCCCAAACT CCTTCCTTCT TCCGATCTCC CACCGTCGCC TGGGCGCCTG 
    GCGGTTTGCT CCACTCCATA GGCGGCCATC GTCTTCTCAA CCAGAAAGCT 
    TCTGGCTGTA CAGCTTGGCG GGATACAAGA CGACGGTCTG CCGTCGGCGA 
    TCCGCAGTCG CCGGAGCTGA AGGTGGAAGC GGCCAACACG GCAGTCCGTG 
    CAGCCGCTTG AGAATTTTTT GTCATGGCAG TGAAAAGATG CGCAGAAGTG 
    CCGATATCTG CTAGGCCGGG AAAGATGATG CGGATTGCTA TTAAGTCTTG 
    GTCAAGGTTC TTGCCCCCTT ACATCCCTGA CGATGTGATC TTCGACATTC 
    TGTTACGGCT GCCATCCAAA TCTCTTATCC GATTTAAGTC CGTGTGTAAG 
    GCTTGGCACG CCATAATATC CAACCCCTGT TTCATCAGTG CTCACCTCGA 
    GTGCTCTAAG CAGAAGCCAT CGATATTTAT GGTCCCTGGT GTTTACGAGA 
    AGCAGAATAA TGGCGAGAAT ACTTCCTTCC TGATGGGCCT CTATCAGTAT 
    CAGGGTGGTA ATATAATGGA ACAAATCCAT GTACAAGACT TTCCACAAGG 
    CATTGGTACA TGGAGTCGTC CGATCCACTG CAATGGCATG CTCCTCATTT 
    CCACCATGAA CCACGAAATG ATTGTCTGCA ACCCATCAAC TAGAGAGATT 
    GTCTCCCTGC CAAAAGGGAG TTATAACCTT CATGCGGGCC CAAGGGCTGG 
    ATTTGGGTTT GACCCTCACA GCAACAAGTA TAAGGTGGCT AGATTTTTCT 
    ACCAGAGGGA CGATGACACA TCAGAATTGG TCTGCAAGTT TGAAGTGCTT 
    ACTCTAGGCA CTAATTTGTG GAGGCAAACT GAGGATCCGC CATATCCAAT 
    TAGTGGGTTA ACTCCTGTTC ATGTGAAAGG CGCCATCTAC TGGATGGTTA 
    ATACGCCACT TTGCCCTGAT CCACCCAATG CATTTCTTAG GTTCTGTTTG 
    ACTAACGAGA AGTTTAGTCT GCTTCAGTAT CCTCCATGTA ATCTAAAGCC 
    TACCCGTTTC ATAGAAGTGG AGGGTGAACT ATGCTGTGCT TGCTTTTGCA 
    GTCAGGTGAG CGCGTTGAAG ATCTGGACCT GCAATTATGC ACAGAACCCT 
    GAATGGACTC AGCGCTGCAC TGTACAGATT CCACCTGATA TAGTTGTGAA 
    TAATCCTGTA GCAAGGCCTC CTATAGTTTT TCTTCATGGA AAAAAGCTGC 
    TACTGACATG GAATCAGGTC TACCAGTATG ACATTCAAAC CTGTAGAATG 
    GAAAAGATAG CTTCAGGGGT TGAAGACTTC ACGTGCTATG ATCCAAGGAA 
    CAATAAGTAC TGGGCATATC TGGAGAAAGA AGTAACGGAT ATGCATCTAT 
    TTAATTATGC TGAGAGTCTA GTTCCGATTA GAGAATTTTG AGAAGTCACT 
    TCATTTTTTG TTAAGATGTG GAGCCTTGCA TTTCAGGCCC AAAGAGTCCT 
    CATGACCGTG TCCCTTTGAA GGAAATGAAA TCAGATTGGC ATGCTTGCCT 
    GGACAGCAAT TTCAAGCAAT ATGCAGTTAC ACAAACACAT CAAATCCCAG 
    CGTTATAATT GGTGCTGGCC TAGTCGCAAA GAAAGCCTGT GAATTGGGCC 
    TTGAGGTTTG CATGTTCGTT CATATATGTA ACTAGTGTAT TGTGTCGTGT 
    GTTTGTGCGA CTATAACCTC CCTCTCTGTA AATATGTATA ATAATTAACT 
    TAATTTGTTA ATAACATTTA ATAATTTAAA TTAGTATTTC CTCCTTTTAT 
    AAG

    Gene models with high sequence identity

    1. AT2G05710.1 [+] show detail - aconitase 3
      E-value: 2e-21; Score: 53.10

     

    InterPro Scan Digest

    Program Description E-value
    superfamily F-box domain, Skp2-like 0.000000000017
    HMMPanther Aconitase/Iron regulatory protein 2/2-methylisocitrate dehydratase 0.00000000001
    HMMPanther Aconitase-like core 0.00000000001
    HMMTigr F-box associated interaction domain 5.4e-19
    ProfileScan F-box domain, cyclin-like 8.941
    HMMPfam F-box domain, cyclin-like 0.00000085
    HMMPfam F-box associated domain, type 1 0.000002
    HMMSmart F-box domain, cyclin-like 0.000000043

    See the detailed result >

    AT2G05710.1

    Model type
    Protein coding
    Short Description
    aconitase 3
    Curator Summary
    Encodes an aconitase that can catalyze the conversion of citrate to isocitrate through a cis-aconitate intermediate, indicating that it may participate in the TCA cycle and other primary metabolic pathways. The protein is believed to accumulate in the mitochondria and the cytosol. It affects CSD2 (At2g28190 - a superoxide dismutase) transcript levels and may play a role in the response to oxidative stress. One member of the family (ACO1 - At35830) was shown to specifically bind to the 5' UTR of CSD2 in vitro. ACO3 is tyrosine-phosphorylated and its phosphorylation state is modulated in response to ABA in Arabidopsis thaliana seeds.
    Computational Description
    aconitase 3 (ACO3); FUNCTIONS IN: aconitate hydratase activity, copper ion binding, ATP binding; INVOLVED IN: in 6 processes; LOCATED IN: cytosol, mitochondrion, cell wall, plasma membrane, chloroplast; EXPRESSED IN: 27 plant structures; EXPRESSED DURING: 16 growth stages; CONTAINS InterPro DOMAIN/s: Aconitase/3-isopropylmalate dehydratase large subunit, alpha/beta/alpha (InterPro:IPR001030), Aconitase family, 4Fe-4S cluster binding site (InterPro:IPR018136), Aconitase A/isopropylmalate dehydratase small subunit, swivel (InterPro:IPR000573), Aconitase/3-isopropylmalate dehydratase large subunit, alpha/beta/alpha, subdomain 2 (InterPro:IPR015932), Aconitase/Iron regulatory protein 2/2-methylisocitrate dehydratase (InterPro:IPR015934), Aconitase-like core (InterPro:IPR015937), Aconitase/3-isopropylmalate dehydratase, swivel (InterPro:IPR015928), Aconitase/iron regulatory protein 2 (InterPro:IPR006249), Aconitase/3-isopropylmalate dehydratase large subunit, alpha/beta/alpha, subdomain 1/3 (InterPro:IPR015931); BEST Arabidopsis thaliana protein match is: aconitase 1 (TAIR:AT4G35830.1); Has 20552 Blast hits to 20369 proteins in 2575 species: Archae - 558; Bacteria - 10777; Metazoa - 503; Fungi - 660; Plants - 232; Viruses - 0; Other Eukaryotes - 7822 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR