K30024

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K30024

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T1  
rosette leaf (PO:0000014)
  • decreased size (PATO:0000587)
    (Rosette leaf::Shape::small)
  • increased width (PATO:0000600)
    (Rosette leaf::Shape::wide)
  • abnormal (PATO:0000460)
    (Rosette leaf::Others)
stem (PO:0009047)
  • increased thickness (PATO:0000591)
    (Stem::Shape::thick)
  • decreased thickness (PATO:0000592)
    (Stem::Shape::thin)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K30024

Determination of introduced cDNA(s)

  • J013071C24 - Rice Full-length cDNAs - Original: J013071C24

    full reading [+] show sequence

    Nucleotide sequence: J013071C24 - full reading 
    TTTCGAAGTA CAGATACAGA AGCAGGCGCT GCTGCTAGAG GCGAAGGATA 
    TAACAAGCGG CGGCCGGAGA CGACGACGGA GAAATCCGGA AGAAGAGGAA 
    CTGCCGGCGC TGAGCTGCAA TCGCGCCCAA GAATCCATCT CCTCCGGCGG 
    CAGCTTACAG GGTCGCTGCT CCATCTCCTT GAATTGTACT AGCTGTCTTG 
    TGCTTAAGAT TTGCGCTCGG CTTCATTTTT GGATTCAAGC TGCTGGGTGT 
    GGATTTTTAT GATGGGCTGT CTACCAACAA AGCAAGTGGG TCGTTCAACA 
    CATTCCTTGA ATCCACGGGA AGCTGTGGCT CTTGCGGCTG AAACATCTTT 
    TACTGTGAAT GAAGTTGAGG CATTATACGA TCTCTTCAGG AAATTAAGTA 
    ATTCAATAAT TAAAGATGGT CTGATTCACA AGGAAGAATT CCATCTTGCT 
    CTCTTTAGGA ACAAGAAGAC AAATCTCTTC GTTGATAGGG TCTTTGATTT 
    GTTTGATCAA AAAGGAAATG GTGTCATCGA ATTTGATGAA TTTGTTCGAT 
    CTCTTAGTGT GTTTCATCCA GATGCACCTG AGGAACAGAA AGCTGGATTT 
    GCATTTAAGT TATATGACCT AAGACAGACA GGGTTTATTG AAAGGCATGA 
    GTTAAAGGAG ATGGTGTTGG CTCTTCTGGA TGAATCAGAT TTGAATATTA 
    CAAGTGATGC TGTCGAGATG ATTGTTGATA GGACATTTGA CCAGGCTGAC 
    ACAAAAGGCG ATGAGAGAAT AGACCAGGAG GAGTGGAATG AGTTTGTCAA 
    AAATAATCCA TATGTGCTGA GGAATATGAC CCTCCCATAC CTCAAGGACC 
    TCACCATGGT ATTCCCAAGT TTTGTGATTC ACTCAGAAGT CAGCGAAGCA 
    GACATGGTTG CCTAACCTGA AACCTGGTGT TGCTACTTGC TAGATTGCAA 
    GTTTGCAACA CTTCTAGCAT GTAATGTTTG TTCCTTGCGT GCATTGTTGA 
    CTAGGGAAAC AGCCATATAG GAGATGAGTT GATGCCTCAA AATGTAGCAT 
    CACTGACCAG CAGATGCTCA CTGTTGAGAT TATGGGCATA TAATGATTTA 
    ATTTATTCCA TAAATAAATC ATGACATT

    Gene models with high sequence identity

    1. AT5G24270.2 [+] show detail - Calcium-binding EF-hand family protein
      E-value: 8e-68; Score: 254.00

     

    InterPro Scan Digest

    Program Description E-value
    ProfileScan EF-HAND 2 12.477
    ProfileScan EF-HAND 2 11.222
    ProfileScan EF-HAND 2 9.297
    HMMPanther Calcineurin B protein 1.1e-109
    HMMPanther CALCINEURIN B 1.1e-109
    Gene3D EF-hand-like domain 9e-48
    superfamily EF-hand 4.5e-43
    FPrintScan Recoverin 6.5e-11
    FPrintScan Recoverin 6.5e-11
    FPrintScan Recoverin 6.5e-11
    FPrintScan Recoverin 6.5e-11
    HMMSmart Calcium-binding EF-hand 1
    HMMSmart Calcium-binding EF-hand 8.6
    HMMSmart Calcium-binding EF-hand 8.4

    See the detailed result >

    AT5G24270.2

    Model type
    Protein coding
    Short Description
    Calcium-binding EF-hand family protein
    Curator Summary
    encodes a calcium sensor that is essential for K+ nutrition, K+/Na+ selectivity, and salt tolerance. The protein is similar to calcineurin B. Lines carrying recessive mutations are hypersensitive to Na+ and Li+ stresses and is unable to grow in low K+. The growth defect is rescued by extracellular calcium.
    Computational Description
    SALT OVERLY SENSITIVE 3 (SOS3); CONTAINS InterPro DOMAIN/s: Recoverin (InterPro:IPR001125), EF-HAND 2 (InterPro:IPR018249), Calcineurin B protein (InterPro:IPR015757), EF-hand-like domain (InterPro:IPR011992), Calcium-binding EF-hand (InterPro:IPR002048); BEST Arabidopsis thaliana protein match is: calcineurin B-like protein 8 (TAIR:AT1G64480.1).
    Link
    InterPro Scan - TAIR