K30028

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K30028

Phenotypes

Morphological phenotypes

Specific phenotypess were NOT observed.

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K30028

Determination of introduced cDNA(s)

  • J013073H04 - Rice Full-length cDNAs - Original: J013073H04

    full reading [+] show sequence

    Nucleotide sequence: J013073H04 - full reading 
    GGTGCAAATG ACGCCGAAGA GAGCTGTGCA TTGCGGTCGG ATCAAATGAT 
    GGCTAGCCAT TTCTACGGCA TGTTCCGGCA AGACGACCCA GCGCCGGCGC 
    CGGCGTGGTG GTTCCAGTCG CTGGCCTTCC TCGGCGCCGC CTACGTCGCC 
    GGCGTCACGC TGCGCCTCCT CGCCTACGTC GCGATCTGCC TCGGCGGGCC 
    CAAGGACCTC CGCCGGTACG GCGCGTGGGC CGTCATCACC GGGCCGACGT 
    CCGGCATCGG CTGCGCCATG GCGCTGGAGC TCGCGCGCCG CGGCCTCAAC 
    CTCGTGCTCG TCGGCCGTGA CCCCGCCAGG CTCCGGGAGA TCTCCGGCAC 
    GATCCGCTCC CGCCACGGCC GGGTGCAGAC CAAGGCCGTG GTGTTCGACC 
    TGTCCCTCGC CTCGACCCCG GACGGCGACC AGCCGCTGCG GCGGCTGCGG 
    GAGGCCGTGG CGGGGCTGGA CGTCGGGGTG GTGGTGAACA ACGCCAGCGA 
    GGGGAGGCCC GGCGCGGTGT ACCTCCACGA GGCGGACGTG GAGGAGTGGG 
    TGAGGATGGC GCGGGTGAAC GTGTCGGCGG TGACAGAGGT GACGGCGGCG 
    GTGCTGCCCG GGATGGTGGA GCGGGGCAGG GGCGGCGCCG TCGTCAACCT 
    CGGCTCAGCG GCGTCGGAGG CCATCCCGTC CTTCCCCCTC TACACCATGT 
    ACGCCTCCAC CAAACGGTAC GTGGCTCAGT TCTCCCGGAG CCTTCACGTC 
    GAGTATGCGA ACAAAGGGAT CCATGTGCAA TGCCAGACGC CGTTCTTCGT 
    GGAGACGACG ATGTTGGCGA AGTTGGAGGA GGAGGTGGGG CTTAGCGTGT 
    CGCCGTTGAA GGTGAGCACG GACACCTACG CGCGCGCGGC GGTGGCGTGG 
    ATCGGACGCG GCGGGCCGCT CTGCACGCCG GGTGGACTGC TGCACCAGCT 
    GATGTGGTGC ATCACCGCCG CCGTGCCGGA GTCCGTCCTC GACTGGATCG 
    TCCTGCGCTT CACCACCTGG AACAGAGGAC GGTAGCTGTT GCAGCCAGCG 
    GAGCAGAGTG TCAAGGACGA GGGGCATGCA AGTTCAGAGA GCTGTGGTGG 
    CTCGCTAAGT TACCATCCTG GCCGCTGTCG TTCGATTACC CTAACGGCGA 
    TCAGCCGTAC GTCGTGACCA CAAGTCGCGC CCGATGTGTG TATCTCGAGC 
    GAGTCAGGGG TGGAGATTAG GTTTGTAAAA ACTATGTCAA AATGTAATTT 
    TACTTTCAAG TATTGATATG GAGTTATGGA CAGTATGTAA TCAACAACTT 
    AATTAGCACT ATTTAATCTA TCGTGATAGA TATGGAATTC AATGC

    Gene models with high sequence identity

    1. AT1G67730.1 [+] show detail - beta-ketoacyl reductase 1
      E-value: 6e-60; Score: 228.00

     

    InterPro Scan Digest

    Program Description E-value
    PatternScan Short-chain dehydrogenase/reductase, conserved site 0
    Gene3D NAD(P)-binding domain 1.7e-39
    HMMPfam Short-chain dehydrogenase/reductase SDR 2.9e-15
    FPrintScan Glucose/ribitol dehydrogenase 1.2e-15
    FPrintScan Glucose/ribitol dehydrogenase 1.2e-15
    FPrintScan Glucose/ribitol dehydrogenase 1.2e-15
    FPrintScan Glucose/ribitol dehydrogenase 1.2e-15
    FPrintScan Glucose/ribitol dehydrogenase 1.2e-15
    superfamily NAD(P)-binding domain 8.4e-45
    HMMPanther 17-BETA HYDROXYSTEROID DEHYDROGENASE 2.3e-68
    HMMPanther Short-chain dehydrogenase/reductase SDR 2.3e-68

    See the detailed result >

    AT1G67730.1

    Model type
    Protein coding
    Short Description
    beta-ketoacyl reductase 1
    Curator Summary
    Encodes one of the two Arabidopsis homologues to YBR159w encoding a S. cerevisiae beta-ketoacyl reductase (KCR), which catalyzes the first reduction during VLCFA (very long chain fatty acids, >18 carbon) elongation: KCR1 (At1g67730), KCR2 (At1g24470). Complementation of the yeast ybr159Delta mutant demonstrated that the two KCR proteins are divergent and that only AtKCR1 can restore heterologous elongase activity similar to the native yeast KCR gene.
    Computational Description
    beta-ketoacyl reductase 1 (KCR1); FUNCTIONS IN: oxidoreductase activity, ketoreductase activity, acetoacetyl-CoA reductase activity; INVOLVED IN: very long-chain fatty acid biosynthetic process, embryo development, cuticle development; LOCATED IN: endoplasmic reticulum, membrane; EXPRESSED IN: 26 plant structures; EXPRESSED DURING: 14 growth stages; CONTAINS InterPro DOMAIN/s: Short-chain dehydrogenase/reductase, conserved site (InterPro:IPR020904), NAD(P)-binding domain (InterPro:IPR016040), Glucose/ribitol dehydrogenase (InterPro:IPR002347), Short-chain dehydrogenase/reductase SDR (InterPro:IPR002198); BEST Arabidopsis thaliana protein match is: beta-ketoacyl reductase 2 (TAIR:AT1G24470.1); Has 86831 Blast hits to 86679 proteins in 3373 species: Archae - 739; Bacteria - 59178; Metazoa - 5127; Fungi - 3946; Plants - 2216; Viruses - 0; Other Eukaryotes - 15625 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR