K30205

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K30205

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T1  
whole plant (PO:0000003)
  • decreased height (PATO:0000569)
    (Adult plant::Plant height::short)
rosette leaf (PO:0000014)
  • decreased size (PATO:0000587)
    (Rosette leaf::Shape::small)
  • increased width (PATO:0000600)
    (Rosette leaf::Shape::wide)
  • abnormal (PATO:0000460)
    (Rosette leaf::Others)
flower (PO:0009046)
  • abnormal (PATO:0000460)
    (Flower::Others)
T2 seed (PO:0009010)
  • low brightness (PATO:0000327)
    (Seed::Color::dark)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K30205

Determination of introduced cDNA(s)

  • J033124I10 - Rice Full-length cDNAs - Original: J033124I10

    full reading [+] show sequence

    Nucleotide sequence: J033124I10 - full reading 
    AAAAAACCTC ATAGCCTCAT CAGCTGATCA CTGAGTGCCT TCTCCTTCTC 
    CTCCTCCTTT AAGAACCCTC TTCCCTGCCC ATCCTTTTCT TCATCCCCTC 
    AACTTTGCCT AAAAGGCAAG AACAAAGCCA TATGATTCTC TGATCCCTTC 
    TTCCAAGCTG CATCCCAACA CAACACATTG TGGTGACACA GCAAACATAA 
    TATACACACA TCCTCTTCTT CTTCTACCAC CCTCCATATT CCTGCAAGAA 
    ACAACTGGAG GAAGAATCAC TACTCCAACA CCATGGAGGT TGCCATGGTC 
    TGCACAAGAG TTAACCTGCT CATCCTCATC CTTTCTCTCT GCTCCCCATA 
    CAAGTTTATA CAGAGCCCCA TGGACTTTGG CCCTTTGAAC CTCCTTCCCA 
    CCACCACCAC TGCATCCAGT GACTTTGGTA GAATCCTCTT CCACTCCCCA 
    TCTGCAGTTC TAAAGCCCCA AGCTCCAAGG GATATATCCC TGCTTCTCAG 
    CTTCCTCTCT GCCTCACCTC TTGGCAAGGT GACGGTGGCA GCCAGGGGAG 
    CAGGTCACTC CATCCATGGA CAGGCACAGG CCCTTGATGG CATTGTAGTG 
    GAGATGAGCT CCTTGCCTTC TGAGATTGAA TTCTACAGAA GAGGAGAAGG 
    AGATGTTTCT TATGCTGATG TGGGTGGTGG AATCATGTGG ATTGAGCTTC 
    TGGAGCAGAG CTTGAAGCTT GGGCTGGCTC CAAGGTCTTG GACTGATTAC 
    CTCTATCTCA CCATTGGTGG AACCTTGTCC AATGCTGGCA TCAGTGGGCA 
    GACATTCAAG CATGGACCTC AGATTAGCAA TGTTCTACAG CTGGAAGTAG 
    TCACAGGCAG GGGTGAGATT GTGACCTGTT CACCCACCAA GGATGCAGAG 
    CTATTCAATG CTGTTCTTGG AGGCCTTGGG CAGTTCGGCA TCATTACTAG 
    GGCAAGGATC CTGCTGCAGG AAGCTCCACA GAAAGTGAAG TGGGTTAGAG 
    CCTTCTATGA TGATTTCGCC ACCTTCACCA AAGACCAGGA GTTGCTGGTG 
    TCAATGCCGG TTTTGGTGGA CTATGTGGAA GGTTTCATCG TGTTGAATGA 
    GCAATCCCTT CACAGCTCCT CCATTGCCTT CCCTACAAAT GTTGACTTCA 
    ACCCAGATTT TGGCACCAAG AACAACCCTA AGATCTACTA CTGCATAGAG 
    TTTGCTGTCC ACGATTACCA GAACAAGAAC ATCAATGTGG AACAGGTCGT 
    GGAAGTCATC TCAAGGCAGA TGAGCCACAT AGCATCCCAC TTGTATAGCG 
    TGGAGGTGTC CTACTTTGAT TTTCTCAACA GGGTTAGGAT GGAGGAGATG 
    AGCCTTAGGA ACAGTGGGCT CTGGGAGGTG CACCATCCAT GGTTGAACAT 
    GTTCGTGCCA AGCGCTGGGA TTAGTGACTT CAGGGACCTG CTCATGGACA 
    GCATCTCACC GGATAACTTC GAGGGCCTCA TCCTCATCTA CCCACTCCTC 
    AGACACAAGT GGGACACCAA CACGTCGGTC GTGTTACCGG ACTCCGGGTC 
    GACGGACCAG GTGATGTACG CGGTGGGCAT CCTCCGGTCG GCGAACCCCG 
    ACGACGGGTG CTCCCACCAC TGCCTCCAGG AGCTCCTCCT CCGCCACCGC 
    CGCCTCGCCG GCGCCGCCGC GTCGGGCCTC GGCGCGAAGC AGTACCTCGC 
    CCACCACCCG ACCCCGGCCG GCTGGCGCCG CCACTTCGGC CGGCGGTGGG 
    AGCGGTTCGC CGATCGCAAG GCCCGGTTCG ACCCGAGGTG CATCCTCGGG 
    CCAGGCCAGG GTATATTCCC CAGGGACAGC AGCAGCAGCA ATGGCGCATT 
    TGCAAGCTAT AGCTGACACG AGAAAGTGCA GTGTAGTGCT GTGCTGTGCC 
    GTGCCGTGCC GTGCCGTGAC AGCTGGTGGT ACAATAAGAG GGGGACATAG 
    GATAGGCAGG CGAGGCTAGG AGGGAGATCT TGTTCTTTCT TTTCGATAGC 
    TTTCTTTTCT GCTTCGATTT GAGGCCGGCA TCGTTTTGGT TGGTTGATGT 
    GGTTGGGCGG GGGTTGATTT TGAACGGATC CTCGGTGGTG GTGATCACCA 
    TCATCACTGG GAATTTTGGA ACTTTGTACA GGCTGAGGAA GCAAGAACAG 
    CTCTAGGGCG AAAGGATAGT GATAGCGATT CTATTTCGAC C

    Gene models with high sequence identity

    1. AT2G41510.1 [+] show detail - cytokinin oxidase/dehydrogenase 1
      E-value: 3e-108; Score: 389.00

     

    InterPro Scan Digest

    Program Description E-value
    ProfileScan FAD-binding, type 2 15.494
    HMMPanther GULONOLACTONE OXIDASE 2.9e-11
    superfamily FAD-linked oxidase-like, C-terminal 5e-74
    superfamily FAD-binding, type 2 1.6e-40
    HMMPfam Cytokinin dehydrogenase 1, FAD/cytokinin binding domain 1.8e-104
    HMMPfam FAD linked oxidase, N-terminal 5.4e-22

    See the detailed result >

    AT2G41510.1

    Model type
    Protein coding
    Short Description
    cytokinin oxidase/dehydrogenase 1
    Curator Summary
    It encodes a protein whose sequence is similar to cytokinin oxidase/dehydrogenase, which catalyzes the degradation of cytokinins.
    Computational Description
    cytokinin oxidase/dehydrogenase 1 (CKX1); FUNCTIONS IN: cytokinin dehydrogenase activity; INVOLVED IN: N-terminal protein myristoylation, cytokinin catabolic process, meristem development; LOCATED IN: vacuole; EXPRESSED IN: lateral root, shoot apex, hypocotyl, root, flower; CONTAINS InterPro DOMAIN/s: Cytokinin dehydrogenase 1, FAD/cytokinin binding domain (InterPro:IPR015345), FAD-binding, type 2 (InterPro:IPR016166), Oxygen oxidoreductase covalent FAD-binding site (InterPro:IPR006093), FAD-linked oxidase-like, C-terminal (InterPro:IPR016164), FAD linked oxidase, N-terminal (InterPro:IPR006094); BEST Arabidopsis thaliana protein match is: cytokinin oxidase/dehydrogenase 6 (TAIR:AT3G63440.1); Has 6769 Blast hits to 6763 proteins in 1376 species: Archae - 168; Bacteria - 3882; Metazoa - 142; Fungi - 1302; Plants - 645; Viruses - 0; Other Eukaryotes - 630 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR