K30539

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K30539

Phenotypes

Morphological phenotypes

Specific phenotypess were NOT observed.

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K30539

Determination of introduced cDNA(s)

  • J013113L03 - Rice Full-length cDNAs - Original: J013113L03

    full reading [+] show sequence

    Nucleotide sequence: J013113L03 - full reading 
    GATAGTTTTG GCGACTCGGC GAGTAACCGT TCCAAATCGC TCTCAACCGC 
    GCCATGGCCG GCTAGACTGC ATGCTAATGC TACACATTTA TATTGTACAA 
    CCCCGCCGGT GGGGAGGAAG AGAGAGCGAG AGAGCGAATC ATCATGGCTA 
    GTCATGTCTT CATGATCCGG CAAGAGACGG CGCCGGCGCA GTGGTGGTTC 
    CTCTCGCTGG CTTTCGTCGG CGCCGCCTAC GCCGCCACCG TCACGCTCCG 
    TTTCGTCGCC TACCTCGCGC TCTGCCGGTG TCACCGGCCG AAGGACGACC 
    TCCGCCGCCG GTACGGCGAA TGGGCCGTCG TCACCGGGCC AACGTCCGGC 
    ATCGGCCGTG CCATGGCGCT GGAGCTCGCG CGCCACGGCC TCAACCTCGT 
    GCTCGTCGGC CGTGACCCAG CCATCCTCCG GGAGATCTCC GGCACGGTCA 
    GGTCTCTCCA CAAAGTGAAG ACCAAGACCG TGGTGTTTGA CCTGTCCCTC 
    GTCTGGACCC CCGATGGTGA TGAGCCGCTG CGGCGGCTGC GGGAGGCGGT 
    GGAGGGGCTG GACGTCGGGG TGGTGGTGAA CAACGCCGGC GTGGCGAAGC 
    CCGGCGCCGT CTACCTGCAC GAGGCGGACG TGGAGGCGTG GGTGAGGATG 
    GTGCGGGTGA ACATGTCGGC AGTGACGGAG GTGACGGCGG TGGTGCTACC 
    TGGGATGGTG TCGCGGGGTA GGGGCGCCAT CGTCAACATC GGCTCGGCTG 
    GGTCGGAGTA CATCCCGACC TTACCTCTCT ACACCATGTA CGCCGCCACC 
    AAACGGTACG TGGCTCAGTT CTCCCGGAGC CTTCACGTCG AGTATGCAAG 
    TAAAGGGATC CATGTGCAGT GCCAGGCGCC GTTCTTCGTG GATACGAGGC 
    TGATGTTCAG GTTCGAGGAG GCGGCGGGCG GCGTATCGCT GTTCACGGTG 
    ACCCCTGACG CGTACGCGCG CGCGGCGGTG GCGTGGATCG GCCGCGGCGG 
    CGCGCTGTGC ACGCCGGCCG TCCGGCACCA GCTGCTACGG CGCATGGCCG 
    CCGCCGCGCC GGACTCCGTC CACGACTGGA TCCTCCTGCG CTTAGCCACC 
    TGGAACAGAA AACGTTGTTT CAGCGGATCT GAGTGACAGA TGACAGATTA 
    TGTGATGCTA AAGAATTGCC AACAAATAAA TAATGTGTCT TCGAAAAGTG 
    CAATTGCCG

    Gene models with high sequence identity

    1. AT1G67730.1 [+] show detail - beta-ketoacyl reductase 1
      E-value: 2e-54; Score: 210.00

     

    InterPro Scan Digest

    Program Description E-value
    superfamily NAD(P)-binding domain 1.9e-47
    FPrintScan Glucose/ribitol dehydrogenase 6e-20
    FPrintScan Glucose/ribitol dehydrogenase 6e-20
    FPrintScan Glucose/ribitol dehydrogenase 6e-20
    FPrintScan Glucose/ribitol dehydrogenase 6e-20
    FPrintScan Glucose/ribitol dehydrogenase 6e-20
    FPrintScan Short-chain dehydrogenase/reductase SDR 1.6e-07
    FPrintScan Short-chain dehydrogenase/reductase SDR 1.6e-07
    FPrintScan Short-chain dehydrogenase/reductase SDR 1.6e-07
    Gene3D NAD(P)-binding domain 5.2e-43
    HMMPanther 17-BETA HYDROXYSTEROID DEHYDROGENASE 1.2e-72
    HMMPanther Short-chain dehydrogenase/reductase SDR 1.2e-72
    HMMPfam Short-chain dehydrogenase/reductase SDR 3.2e-18

    See the detailed result >

    AT1G67730.1

    Model type
    Protein coding
    Short Description
    beta-ketoacyl reductase 1
    Curator Summary
    Encodes one of the two Arabidopsis homologues to YBR159w encoding a S. cerevisiae beta-ketoacyl reductase (KCR), which catalyzes the first reduction during VLCFA (very long chain fatty acids, >18 carbon) elongation: KCR1 (At1g67730), KCR2 (At1g24470). Complementation of the yeast ybr159Delta mutant demonstrated that the two KCR proteins are divergent and that only AtKCR1 can restore heterologous elongase activity similar to the native yeast KCR gene.
    Computational Description
    beta-ketoacyl reductase 1 (KCR1); FUNCTIONS IN: oxidoreductase activity, ketoreductase activity, acetoacetyl-CoA reductase activity; INVOLVED IN: very long-chain fatty acid biosynthetic process, embryo development, cuticle development; LOCATED IN: endoplasmic reticulum, membrane; EXPRESSED IN: 26 plant structures; EXPRESSED DURING: 14 growth stages; CONTAINS InterPro DOMAIN/s: Short-chain dehydrogenase/reductase, conserved site (InterPro:IPR020904), NAD(P)-binding domain (InterPro:IPR016040), Glucose/ribitol dehydrogenase (InterPro:IPR002347), Short-chain dehydrogenase/reductase SDR (InterPro:IPR002198); BEST Arabidopsis thaliana protein match is: beta-ketoacyl reductase 2 (TAIR:AT1G24470.1); Has 86831 Blast hits to 86679 proteins in 3373 species: Archae - 739; Bacteria - 59178; Metazoa - 5127; Fungi - 3946; Plants - 2216; Viruses - 0; Other Eukaryotes - 15625 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR