K31412

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K31412

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T1  
cauline leaf (PO:0000013)
  • abnormal (PATO:0000460)
    (Cauline leaf::Others)
rosette leaf (PO:0000014)
  • abnormal (PATO:0000460)
    (Rosette leaf::Others)
fruit (PO:0009001)
  • abnormal (PATO:0000460)
    (Silique::Others)
stem (PO:0009047)
  • decreased thickness (PATO:0000592)
    (Stem::Shape::thin)
  • desaturated green (PATO:0001272)
    (Stem::Color::pale)
T2 seed (PO:0009010)
  • increased size (PATO:0000586)
    (Seed::Shape::large)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K31412

Determination of introduced cDNA(s)

  • J013108N11 - Rice Full-length cDNAs - Original: J013108N11

    full reading [+] show sequence

    Nucleotide sequence: J013108N11 - full reading 
    GATCTCTCCT TTTTTTTTTC TCCCCAAATC ACCCCTCCGC CGAAACGGGA 
    GCTCGATCTC CGCCGCCACC GCCAATCCCC GAACCCTACC TCTCCCCCCC 
    TCCACCCATG GCGCTCTCCC GCCGCCTCCT CCCGCTCCTC CTCCGCCGCG 
    GGTCCACCCC CTCCCTCTCC CCCGCCCGCG CGCTCTCCAC GGCCGCCGTG 
    ACGGCCGATG CTCCCGCCGC GGCGGCGGCG GCGGCGGAGG AGGAGGCGAT 
    GACCATCAAG GGCGTGCGCA TCTCGGGGCG GCCGCTGTAC ATGGACATGC 
    AGGCGACCAC CCCCGTCGAC CCGCGGGTGC TCGACGCCAT GCTCCCCTTC 
    TACCTCTCCC GCTACGGGAA CCCCCACTCC CGCACCCACC TCTACGGCTG 
    GGAGTCCGAC GCCGCCGTCG AGGAGGCCCG CGCCCGCGTC GCCTCCCTCG 
    TCGGCGCCGA CCCCAGGGAG ATCTTCTTCA CCTCCGGCGC CACCGAGTGC 
    AACAACATCG CCGTCAAGGG CGTCATGCGC TTCTACCGCG ACCGCCGCCG 
    CCACGTCGTC ACCACGCAGA CCGAGCACAA GTGCGTCCTC GACTCCTGCA 
    GGTACCTCCA GCAGGAAGGC TTCGAGGTCA CCTACCTCCC CGTGCGCCCC 
    GACGGCCTCG TCGACGTCGC GCAGCTCGCC GACGCCATCC GCCCCGACAC 
    CGGCCTCGTC TCCGTCATGG CCGTCAACAA CGAGATCGGC GTCGTGCAGC 
    CACTCGAGGA GATTGGTCGA ATCTGCAAGG AGAAGGGCGT GCCCTTCCAT 
    ACTGATGCTG CCCAGGCGCT CGGGAAGATC CCGATTGATG TCAACCAAAT 
    GGGGATTGGG CTCATGTCTT TGTCTGCGCA CAAGATTTAT GGGCCAAAGG 
    GCGTGGGTGC TCTCTATCTG CGTCGTCGAC CCCGCATTCG TGTGGAGCCG 
    CAGATGAGTG GCGGTGGCCA GGAACGCGGA ATTCGCAGTG GAACAGTGCC 
    AACGCCTCTT GTTGTTGGAT TTGGTGCTGC CTGCGAAATT GCGGCTAAAG 
    AGATGGACTA TGATCACAGG AGAGCCTCGG TTCTGCAGCA GCGCCTTCTT 
    GATGGCATCC GTGGACAGGT TGATGATATT GTTATCAATG GAAGCATGGA 
    GCATCGGTAC CCTGGTAACC TGAATTTGTC ATTTGCATAT GTTGAGGGGG 
    AGAGCTTGCT GATGGGCCTG AAGGAGGTGG CTGTGTCGAG TGGCAGTGCC 
    TGCACTAGTG CCAGCTTGGA GCCCTCTTAT GTGCTGCGGG CACTTGGTGT 
    AGAAGAGGAC ATGGCGCACA CCTCCATTCG CTTTGGCATT GGCCGCTTTA 
    CCACTGAGGA AGAGGTTGAC AGAGCAATTG AGCTCACCGT GCATCAGGTG 
    AAGAAGCTTC GTGACATGAG TCCACTGTAT GAGATGGCCA AGGCAGGCAT 
    TGACCTCAAG AGTATCCAGT GGGCGCAGCA CTGAGACAGC TTTCACTTAG 
    AGCTAATCCC TGGCTGCTTT TGAAGTGCAC GGAAGGACAC ATAGATAGGC 
    CTGTCTGGTG TTGGTGAAGA CATTTCTATG AACCATGTGA TAGTTCTGTC 
    AAGACAATCA GCTAGTATTG TACATCTGTT CACTTGCCAA GTTGCCAGCT 
    CTCAGGCTGG CAACTAATGT TTTCTGCTAG GTTAGCACCT TGTTCTCTAG 
    CTAGAATACC CCAACACGAA ATAAGAATGC TTGAGTGGTT ACTGTGTTGC 
    CTGAAACCCT TTAAGATACT AATTGGAGTT ACTGGCATAA TGCTGTTACT 
    ATATATTTTG GTGCTC

    Gene models with high sequence identity

    1. AT5G65720.1 [+] show detail - nitrogen fixation S (NIFS)-like 1
      E-value: 0; Score: 633.00

     

    InterPro Scan Digest

    Program Description E-value
    Gene3D Pyridoxal phosphate-dependent transferase, major region, subdomain 1 5.4e-95
    PatternScan Aminotransferase class-V pyridoxal-phosphate binding site 0
    superfamily Pyridoxal phosphate-dependent transferase, major domain 1.1e-115
    HAMAP Cysteine desulfurase 53.764
    HMMPanther CYSTEINE DESULFURYLASE 1.4e-159
    HMMPfam Aminotransferase, class V/Cysteine desulfurase 2.2e-87
    FPrintScan PRICHEXTENSN 9e-11
    FPrintScan PRICHEXTENSN 9e-11
    FPrintScan PRICHEXTENSN 9e-11
    FPrintScan PRICHEXTENSN 9e-11

    See the detailed result >

    AT5G65720.1

    Model type
    Protein coding
    Short Description
    nitrogen fixation S (NIFS)-like 1
    Curator Summary
    cysteine desulfurase whose activity is dependent on AtSufE activation.
    Computational Description
    nitrogen fixation S (NIFS)-like 1 (NFS1); FUNCTIONS IN: transaminase activity, zinc ion binding, cysteine desulfurase activity, ATP binding; INVOLVED IN: iron-sulfur cluster assembly; LOCATED IN: mitochondrion; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Pyridoxal phosphate-dependent transferase, major domain (InterPro:IPR015424), Cysteine desulfurase (InterPro:IPR010240), Aminotransferase, class V/Cysteine desulfurase (InterPro:IPR000192), Pyridoxal phosphate-dependent transferase, major region, subdomain 1 (InterPro:IPR015421), Cysteine desulfurase, NifS (InterPro:IPR016454); BEST Arabidopsis thaliana protein match is: chloroplastic NIFS-like cysteine desulfurase (TAIR:AT1G08490.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR