K31425

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K31425

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T2 seed (PO:0009010)
  • low brightness (PATO:0000327)
    (Seed::Color::dark)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K31425

Determination of introduced cDNA(s)

  • J033027F19 - Rice Full-length cDNAs - Original: J033027F19

    full reading [+] show sequence

    Nucleotide sequence: J033027F19 - full reading 
    GGCGTTCGAT TCGATTCGCA TCGGCGTTGC TTCCAACGAG AGGGAGGGAG 
    GGAGGAGAGG CGAATCGCGG CGAGCGGCGG CGGCGGCGGC GATGGTGGAC 
    GTGTCGAGGG TGCAGAAGGA GCTGACGGAG TGCAACCGGG ACAGGGAGGT 
    CTCCGGGGTG TCCATCGCGC TCCACGACGG CTCCACCATC TCCCACCTCA 
    CCGGCACCAT CGCCGGGCCC CAAGGCACGC CCTACGAGGG CGGCACCTTC 
    GTCATCGACA TCCGCCTCCC AGGTGGTTAC CCCTTTGAGC CTCCTAAGAT 
    GCAGTTTATC ACCAAAGTAT GGCACCCTAA CATTAGCAGC CAAAACGGAG 
    CAATCTGCTT GGACATACTG AAGGACCAAT GGAGCCCAGC CCTTACATTG 
    AAGACGGCAC TGCTTTCCCT CCAAGCTCTG CTTTCTGCCC CTGCACCTGA 
    TGATCCTCAG GATGCTGTCG TTGCACAACA GTACTTGCGT GACTATTCCA 
    CATTTTCTGC TACTGCTCGC TACTGGACGG AGGCCTTTGC AAAGAATTCC 
    TCCACCGGCA TGGAAGAAAA GGTGCAGAAG CTGGTTGAGA TGGGCTTCCC 
    TGAAGACATG GTGAGAAGTG TTCTGAAGAG TGTCAATGGC GACGAGAACA 
    TGGCTCTTGA GAAGCTCTGC TCTGGCTGAG GCCCTATAAG CCGATATGAA 
    CTGTAAATTG AATCCCCATT CGTTATAAGA CAACAGTGCT TGCTAATGCT 
    CCTGTTCTGC CATTTAAGTG TGGCTTTATG ACACTCTTCT ATATAGGGGA 
    TTGGACCATG GGACGTTGGG ACTGAACTGA ACCTATCCTA ACTTGACAGA 
    GTTCGGTCAT TG

    Gene models with high sequence identity

    1. AT5G50870.1 [+] show detail - ubiquitin-conjugating enzyme 27
      E-value: 4e-55; Score: 211.00

     

    InterPro Scan Digest

    Program Description E-value
    superfamily Ubiquitin-conjugating enzyme/RWD-like 1.8e-51
    superfamily UBA-like 3.6e-07
    ProfileScan Ubiquitin-associated/translation elongation factor EF1B, N-terminal, eukaryote 12.451
    ProfileScan Ubiquitin-conjugating enzyme, E2 38.221
    HMMSmart Ubiquitin-conjugating enzyme, E2 9.5e-61
    HMMSmart Ubiquitin-associated/translation elongation factor EF1B, N-terminal, eukaryote 3.7e-07
    HMMPfam Ubiquitin-conjugating enzyme, E2 1.8e-44
    HMMPfam Ubiquitin-associated/translation elongation factor EF1B, N-terminal 1e-08
    HMMPanther UBIQUITIN-CONJUGATING ENZYME E2-25KD 6e-84
    HMMPanther UBIQUITIN-CONJUGATING ENZYME E2 6e-84
    PatternScan Ubiquitin-conjugating enzyme, E2 0
    Gene3D Ubiquitin-conjugating enzyme/RWD-like 1.5e-48

    See the detailed result >

    AT5G50870.1

    Model type
    Protein coding
    Short Description
    ubiquitin-conjugating enzyme 27
    Computational Description
    ubiquitin-conjugating enzyme 27 (UBC27); FUNCTIONS IN: ubiquitin-protein ligase activity; INVOLVED IN: ubiquitin-dependent protein catabolic process; LOCATED IN: cellular_component unknown; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 14 growth stages; CONTAINS InterPro DOMAIN/s: Ubiquitin-conjugating enzyme/RWD-like (InterPro:IPR016135), Ubiquitin-associated/translation elongation factor EF1B, N-terminal, eukaryote (InterPro:IPR015940), Ubiquitin-associated/translation elongation factor EF1B, N-terminal (InterPro:IPR000449), Ubiquitin-conjugating enzyme, E2 (InterPro:IPR000608), UBA-like (InterPro:IPR009060); BEST Arabidopsis thaliana protein match is: ubiquitin conjugating enzyme 8 (TAIR:AT5G41700.4); Has 10156 Blast hits to 10152 proteins in 398 species: Archae - 0; Bacteria - 2; Metazoa - 4517; Fungi - 2174; Plants - 1846; Viruses - 24; Other Eukaryotes - 1593 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR