K31642

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Phenotypes

Morphological phenotypes

Generation	Anatomical entity /structure development stage	Phenotypes
T2	seed (PO:0009010)	increased width (PATO:0000600) (Seed::Shape::wide)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K31642

Determination of introduced cDNA(s)

J023078F10 - Rice Full-length cDNAs - Original: J023078F10

full reading [+] show sequence

GACGCGACCC AAGCCGATCC GCCCGCACCC GCACACACAT CGCTCGGTCG 
AAGGAGGAGG TTCTAGAAAC TTCTGGAAGC GGTGGAGGTG TGGGGAGAGA 
GAGAGAGAGA GAGAGAGAGA GAGAGAGGCA GAGAGCCATG GCGCGGAAGA 
AGATCCGGGA GTACGACTCC AAGCGCCTCC TAAGGGAGCA CCTCAAGCGC 
CTCGCCGCCA TCGACCTCCA CATCCTCTCC GCCCAGGTCA CGGAATCAAC 
TGATTTCACA GAGCTCGTCA ACCAAGAGCC ATGGCTCTCG TCTATGAAGT 
TGGTTGTGAA ACCCGACATG CTATTTGGCA AACGTGGGAA GAGTGGCCTT 
GTGGCCCTCA ACCTAGATCT TGCTCAAGTC CGCCAATTCG TCAAAGAGCG 
GTTGGGAGTT GAGGTTGAGA TGGGTGGCTG CAAGGCTCCT ATTACAACAT 
TCATAGTTGA GCCATTTGTT CCACATGATC AAGAGTACTA TCTTTCTATT 
GTATCAGAGA GGCTTGGTTC CACCATTAGC TTCTCGGAGT GTGGAGGTAT 
TGAGATCGAG GAGAACTGGG ATAAGGTCAA GACAGTTTTT CTTCCCACCG 
AGAAAGCAAT GACACCTGAT GCGTGTGCTC CATTGATTGC CACCCTACCG 
TTAGAGGTTC GGACAAAAAT AGGTGATTTC ATCAGAGGTG TATATTCTGT 
TTTCCAAGAC TTGGATTTCT CATTCCTTGA GATGAATCCG TTCACCATGG 
TGAATGGGGA ACCATATCCT CTAGACATGA GAGGAGAATT GGACGACACA 
GCTGCCTTTA AGAACTTTAA GAAGTGGGGA AACATTCAGT TCCCTCTGCC 
TTTCGGAAGA GTCCTCAGCC CCTCTGAAAG CTTTATCCAT GAACTGGATG 
AGAAGACAAG CTCATCGCTC AAATTCACAG TCCTGAACCC GAAAGGGCGC 
ATTTGGACAA TGGTTGCAGG TGGTGGTGCT AGTGTCATAT ATGCTGATAC 
TGTTGGAGAT TTGGGATATG CGTCAGAGCT TGGAAATTAT GCAGAATACA 
GCGGCGCTCC CAACGAGGAG GAGGTTCTGC AGTATGCTAG AGTGGTTTTG 
GATTGTGCCA CTGCTGATCC TGATGGCCGT AAGAGAGCTC TTCTCATTGG 
AGGTGGTATA GCGAACTTCA CTGATGTCGC TGCTACATTC AGTGGCATCA 
TTCGAGCTTT AAGAGAGAAG GAATCCAAAT TGAAGGCTGC ACGGATGAAC 
ATTTACGTTC GGAGAGGTGG TCCAAACTAC CAAACTGGCC TTGCCAAAAT 
GCGTACACTA GGTGCAGAAC TTGGTGTTCC AATTGAGGTA TATGGACCAG 
AGGCAACAAT GACTGGAATC TGCAAGCAAG CCATTGATTG CATCATGGCT 
GAAGCATAAT TCAGACAACT ATTTGCGCTG TTCCCAGCTT GAGTCCATTT 
TGTTTCAGAA ATTGTCAGTG TGAGGTGTTG CCTTTCTCTC TGAGGGAATG 
TGTGCTGTTG TTGGTGTAAA ACAAAACAAA GAAAGATCGT ATTGTTAGAA 
TAAACTACAT CTGTAAGTTT GTAACGTAAT TACGCAATAA TGTTAATGTT 
TGTTTCTAGT TCTGAATTCT CTGTGCCTTG GTTGGTGGCC TTATCCACCA 
AACTCAAAGA GGGTTCTTGA TTTTGTCATG TATGAGAGAC AGTGAAATGG 
AGGGCTTAGT GACAGGAACT GCGTTGTTGT GGCTTGATT

Gene models with high sequence identity

AT1G09430.1 [+] show detail - ATP-citrate lyase A-3
E-value: 0; Score: 748.00

InterPro Scan Digest

Program	Description	E-value
HMMPanther	ATP-CITRATE SYNTHASE	5.7e-166
HMMPfam	ATP-grasp fold, succinyl-CoA synthetase-type	4.4e-18
superfamily	Glutathione synthetase ATP-binding domain-lik	1.3e-36
superfamily	Succinyl-CoA synthetase-like	5.7e-20
Gene3D	ATP-grasp fold, subdomain 2	3.5e-19

See the detailed result >

AT1G09430.1

Model type: Protein coding
Short Description: ATP-citrate lyase A-3
Curator Summary: Encodes subunit A of the heteromeric enzyme ATP citrate lyase (ACL). In animals, ACL is encoded by a single gene; ACL in Arabidopsis is composed of two polypeptides, ACLA (encoded by 3 genes) and ACLB (encoded by 2 genes). The holoenzyme has an A(4)B(4)stoichiometry. Expression of both ACLA and ACLB but not of either of the subunits alone results in ACL activity.
Computational Description: ATP-citrate lyase A-3 (ACLA-3); CONTAINS InterPro DOMAIN/s: ATP-grasp fold, subdomain 2 (InterPro:IPR013816), ATP-grasp fold, succinyl-CoA synthetase-type (InterPro:IPR013650), Succinyl-CoA synthetase-like (InterPro:IPR016102); BEST Arabidopsis thaliana protein match is: ATP-citrate lyase A-1 (TAIR:AT1G10670.4); Has 5303 Blast hits to 5302 proteins in 1635 species: Archae - 133; Bacteria - 3235; Metazoa - 230; Fungi - 139; Plants - 96; Viruses - 0; Other Eukaryotes - 1470 (source: NCBI BLink).
Link: InterPro Scan - TAIR