K31940

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K31940

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T2 seed (PO:0009010)
  • increased width (PATO:0000600)
    (Seed::Shape::wide)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K31940

Determination of introduced cDNA(s)

  • J013001B17 - Rice Full-length cDNAs - Original: J013001B17

    full reading [+] show sequence

    Nucleotide sequence: J013001B17 - full reading 
    GGCTCTCCTC CTCCGCCGCC GCCGCCGCAA CCAACCAGCC AGCCAGCCAT 
    GCAGCCCGAC TCCGCCTCCC ACCGCATCGC CCGCGTCGCC GCCCACCTCA 
    GCCCGTCGCC GCGCCCCCAG ATGGAGGAGG GGGTGAGGCC GGCGCCATGC 
    AGGGCCAAGG GCGGCGCGCC GGGGTTCAAG GTGGCCGTGC TGGGCGCGGC 
    GGGAGGGATC GGGCAGCCGC TGTCGCTGCT CATGAAGTTG AACCCGCTCG 
    TCTCTGTGCT CCACCTCTAT GATGTCGTCA ACACGCCCGG TGTCACCGCC 
    GATGTCAGCC ACATGGACAC CACCGCCGTG GTCCGTGGTT TCCTTGGGCC 
    CAACCAGCTT GAGGCAGCAC TTACAGGAAT GGATCTTGTC ATTATCCCTG 
    CTGGTCTCCC AAGGAAACCA GGAATGACAA GGGATGATTT GTTCAACAAA 
    AACGCTGGGA TTGTTCGCTC ACTTTGTGAA GGCGTTGCCA AGTGCTGTCC 
    AAATGCAATT GTGAACTTGA TCAGCAACCC AGTGAACTCA ACCGTCCCCA 
    TTGCAGCAGA GGTTTTCAAG AAAGCTGGAA CTTATGATCC CAAACGTCTC 
    CTTGGAGTTA CAACACTGGA TGTAGCAAGG GCAAACACCT TTGTGGCTGA 
    AGTACTTGGA ATTGACCCAA AAGATGTCAA TGTTCCAGTT GTTGGTGGGC 
    ATGCTGGAGT GACTATATTA CCCCTCCTCT CCCAGGTCCA CCCCCCATGC 
    TCATTTACCC CAGATGAAAT CAGCTATTTG ACTAAACGCA TACAGAATGG 
    TGGCACAGAA GTTGTTGAGG CAAAGGCAGG AGCAGGCTCT GCAACTTTGT 
    CAATGGCTTT TGCTGCAGCA AAATTTGGTG ATGCATGCTT GCGAGCAATG 
    CGTGGTGATG CTGGCGTTGT GGAATGTTCA TATGTTGCAT CTGCGGTGAC 
    CGAGTTGCCG TTCTTTGCAA CAAAAGTGAG GTTAGGTCGT GCTGGAGCCG 
    AAGAGGTTCT CCCTCTGGGG CCGTTGAATG ATTTTGAGAG AGCTGGCTTG 
    GAGATGGCAA AGAAAGAGCT GATGGAGAGC ATTCAGAAGG GCATCGATTT 
    CATGAACAAG TGAGGTGTAT GAAGGGACAA AGTTATGCAT ACATGTAAAG 
    AGTGTCCTAT ATATAGTGCT CATATACATG TAAAATTTTC GCCGGGCTCA 
    TATACATGTA AAATCACTTC TGTAAAACGA CCGTGAAATG CTAATTACCC 
    TTTAGTTGCA CTAAACCTTT TTTTCCTGAT CATGGAATAA AAATCCTATA 
    CTAGACAACA TCCTGTTGT

    Gene models with high sequence identity

    1. AT5G09660.1 [+] show detail - peroxisomal NAD-malate dehydrogenase 2
      E-value: 1e-158; Score: 556.00

     

    InterPro Scan Digest

    Program Description E-value
    superfamily Lactate dehydrogenase/glycoside hydrolase, family 4, C-terminal 6.2e-54
    superfamily NAD(P)-binding domain 4e-41
    HMMTigr Malate dehydrogenase, NAD-dependent, eukaryote/gamma proteobacteria 8e-175
    HMMPfam Lactate/malate dehydrogenase, C-terminal 2.3e-47
    HMMPfam Lactate/malate dehydrogenase, N-terminal 4.4e-47
    PatternScan Malate dehydrogenase, active site 0
    HMMPIR L-lactate/malate dehydrogenase 7.9e-55
    HMMPanther Malate dehydrogenase, NAD-dependent, eukaryote/gamma proteobacteria 2e-193
    HMMPanther MALATE AND LACTATE DEHYDROGENASE 2e-193
    Gene3D NAD(P)-binding domain 1.3e-41
    Gene3D Lactate dehydrogenase/glycoside hydrolase, family 4, C-terminal 3e-59

    See the detailed result >

    AT5G09660.1

    Model type
    Protein coding
    Short Description
    peroxisomal NAD-malate dehydrogenase 2
    Curator Summary
    encodes a microbody NAD-dependent malate dehydrogenase encodes an peroxisomal NAD-malate dehydrogenase that is involved in fatty acid beta-oxidation through providing NAD to the process of converting fatty acyl CoA to acetyl CoA.
    Computational Description
    peroxisomal NAD-malate dehydrogenase 2 (PMDH2); FUNCTIONS IN: malate dehydrogenase activity; INVOLVED IN: regulation of fatty acid beta-oxidation, regulation of photorespiration; LOCATED IN: in 6 components; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 14 growth stages; CONTAINS InterPro DOMAIN/s: Lactate/malate dehydrogenase, N-terminal (InterPro:IPR001236), Lactate/malate dehydrogenase, C-terminal (InterPro:IPR022383), NAD(P)-binding domain (InterPro:IPR016040), Malate dehydrogenase, NAD-dependent, eukaryote/gamma proteobacteria (InterPro:IPR010097), L-lactate/malate dehydrogenase (InterPro:IPR001557), Malate dehydrogenase, active site (InterPro:IPR001252), Lactate dehydrogenase/glycoside hydrolase, family 4, C-terminal (InterPro:IPR015955); BEST Arabidopsis thaliana protein match is: peroxisomal NAD-malate dehydrogenase 1 (TAIR:AT2G22780.1); Has 17070 Blast hits to 17068 proteins in 5416 species: Archae - 235; Bacteria - 12016; Metazoa - 1211; Fungi - 533; Plants - 741; Viruses - 0; Other Eukaryotes - 2334 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR