K32310

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Phenotypes

Morphological phenotypes

Generation	Anatomical entity /structure development stage	Phenotypes
T2	seed (PO:0009010)	increased size (PATO:0000586) (Seed::Shape::large) increased length (PATO:0000573) (Seed::Shape::long)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K32310

Determination of introduced cDNA(s)

J013001K01 - Rice Full-length cDNAs - Original: J013001K01

full reading [+] show sequence

GGGAGGAATC CAGAAGCGGG GGGCTCCCAC CTTGTTGGCG ACGATGCTGC 
GCGCGGGAGG GAGGCGGCTC TTGGCCCCCG GGCTCCGGCG GGTCCTCGGC 
GGCGGGGCGG CCGCTCCGGT GGCCGTGGGC GGGGCGAAGG CGTACCACGA 
GCGGGTGGTG GACCACTACG AGAACCCGCG GAACGTGGGG TCGTTCGAGA 
ACGACGACCC CAGCGTCGGG ACGGGGCTGG TGGGGGCGCC GGCCTGCGGC 
GACGTGATGA AGCTGCAGAT CCGCGTCGAC GAGAGCTCCG GCAAGATCGT 
CGACGCATGC TTCAAGACCT TTGGGTGCGG ATCCGCCATC GCGTCCTCCT 
CCGTCGCTAC TGAATGGGTA AAGGGCAAGC AAATGGAGGA AGTAGTGACT 
ATAAAAAACA CTGAGATTGC CAAGCACTTG TCACTTCCAC CAGTAAAGCT 
CCATTGTAGC ATGCTTGCTG AGGATGCAAT TAAAGCTGCC GTGAAAGACT 
ATGAAGCAAA GAAGGCGAAG CTAGCACAAA AGGGTGAAGA GAAGGCTGCC 
GAAGCATGAG TGCCAGAGGT TCTGTCATGA AGAATGTTGC AGTGCCCTGC 
GTACTCGTAG TAAAGAACCT GTCATGAAGA AGTCTCTGCT CTGCTTCTTG 
CTGGCGGGAA ATAGTGTGTA ACGCTCACAA ACTCTATGCA GCATGTATCA 
TAAGGATGAA GGCAAACATT TTTGAATAAA TGTGCTGTCA TCTTGAAAGA 
AAAAAAAAAG GTGTATGCGT GTGTATGGCC TAGTAATGAA CATATGGTAC 
GGAATTACGG TCGCGAGATA AGGCACATTG TTTCT

Gene models with high sequence identity

AT4G22220.1 [+] show detail - SufE/NifU family protein
E-value: 3e-58; Score: 222.00

InterPro Scan Digest

Program	Description	E-value
Coil	coiled-coil	0
HMMPfam	NIF system FeS cluster assembly, NifU, N-terminal	2.1e-54
superfamily	SufE/NifU	4.4e-45
Gene3D	no description	3.4e-45
HMMTigr	ISC system FeS cluster assembly, IscU scaffold	5.4e-77
HMMPanther	NIF system FeS cluster assembly, NifU, N-terminal	1.1e-71

See the detailed result >

AT4G22220.1

Model type: Protein coding
Short Description: SufE/NifU family protein
Curator Summary: Encodes a mitochondrial protein similar to E.coli IscU. In bacteria, IscU is a scaffold protein accepting sulfur and iron to build a transient Fe-S cluster,which is subsequently transferred to a target apoprotein.
Computational Description: ISU1; CONTAINS InterPro DOMAIN/s: NIF system FeS cluster assembly, NifU, N-terminal (InterPro:IPR002871), ISC system FeS cluster assembly, IscU scaffold (InterPro:IPR011339); BEST Arabidopsis thaliana protein match is: ISCU-like 3 (TAIR:AT4G04080.1); Has 5453 Blast hits to 5453 proteins in 1998 species: Archae - 110; Bacteria - 3654; Metazoa - 148; Fungi - 154; Plants - 89; Viruses - 0; Other Eukaryotes - 1298 (source: NCBI BLink).
Link: InterPro Scan - TAIR