K32335

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K32335

Phenotypes

Morphological phenotypes

Specific phenotypess were NOT observed.

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K32335

Determination of introduced cDNA(s)

  • J023118L15 - Rice Full-length cDNAs - Original: J023118L15

    full reading [+] show sequence

    Nucleotide sequence: J023118L15 - full reading 
    GATATGAGAC TCTGACACGT ACGCACAGCT AGATCGAAGC CCTGGTCGAG 
    TTTATAAATT GAGATATATC CGTCCTCCAT TCTCAGGCCA GTTCTTGCAA 
    GACTCTTGCC GTGTTGAGAC TGCAGAAACG AGAGCTAGCT AGCCAGAGGC 
    TGCAAGAAGA TTCTGCACCC ATAGATCGTC TACCATGGCC ACAAACCCAG 
    GACTCTTCAC GGAGTGGCCA TGGAAGAAGC TTGGCAGCTT CAAGTACGTG 
    TTGCTGGCGC CGTGGGTAGC GCACGGGTGG TATGAGGTGG CGACCAAGGG 
    GTGGCGGGAG GTGGATCTCG GGTACATCGC CATCCTCCCG TCGTTGCTGC 
    TGCGGATGCT GCACAACCAA GCCTGGATCA CCATCTCCCG CCTCCAAAAC 
    GCCCGTGGCC GCCGCCAGAT TGTTCGCCGC GGCATCGAGT TCGACCAGGT 
    TGACCGCGAG AGGAACTGGG ACGACCAGAT CATCCTGAGC GGTATCCTAC 
    TATACCTCGG CGCACTGTAC GTACCGGGCG GGCAACACTT ACCGCTGTGG 
    AGGACGGATG GCGCGGGGCT GATCGCCTTG CTGCATGCGG GGCCAGTGGA 
    GTTCCTCTAC TACTGGTTCC ATCGTGGCGT TGCACCACCA CTTTCTCTAC 
    ACCCACTACC ACTCGCACCA CCACTCTTCA ATCGTCACTG AGCCCATCAC 
    ATCCGTCATC CATCCGTTTG GCTGAGCTCG TTGCCTACGA ATTGCTCTTC 
    TCGATCCCAC TGATCGCCTG TGCCTTGACT GGAACTGCTT CCATAATTGC 
    ATTCGAGATG TATTTGATTT ACATTGATTT CATGAACAAC ATGGGGCACT 
    GCAACTTCGA GTTGGTACCC AGCTGGCTCT TCACATGGTT CCCTCCTCTC 
    AAGTATCTCA TGTACACGCC ATCGTTTCAT TCTCTGCACC ACACTCAGTT 
    TCGGACAAAC TACTCCCTTT TCATGCCGTT CTACGATTAT ATCTACAACA 
    CCATGGACAA GTCATCAGAC ACTCTGTATG AGAACTCACT GAAAAACAAT 
    GAGGAAGAAG AAGCAGTGGA TGTGGTTCAC CTTACACACC TGACCACCCT 
    GCATTCCATC TACCACATGA GGCCCGGTTT CGCCGAATTT GCGTCCAGGC 
    CCTACGTTTC CAGGTGGTAC ATGAGGATGA TGTGGCCTCT GTCATGGCTC 
    TCCATGGTGC TGACATGGAC GTACGGTCCT TCATTCACTG TTGAGAGGAA 
    TGTCATGAAG AAGATCAGGA TGCAGTCATG GGCCATACCA AGATACAGTT 
    TCCATTATGG ATTGGATTGG GAGAAGGAAG CTATCAATGA TCTTATCGAA 
    AAGGCGGTAT GTGAAGCTGA CAAGAATGGG GCTAAAGTTG TAAGCCTTGG 
    ACTCCTGAAT CAGGCACATA CCCTGAATAA AAGTGGAGAA CAATATCTGC 
    TGAAATACCC AAAGTTGGGA GCAAGAATTG TCGATGGAAC CAGCTTAGCT 
    GCTGCAGTGG TTGTCAACAG TATCCCCCAA GGCACAGACC AAGTAATTCT 
    TGCAGGAAAT GTTTCCAAGG TGGCGCGTGC TGTAGCGCAA GCATTATGCA 
    AGAAAAATAT CAAAGTCACG ATGACAAACA AGCAAGATTA TCATTTACTC 
    AAGCCAGAAA TACCAGAAAC TGTAGCTGAC AATCTTTCGT TTTCGAAAAC 
    AGGCACCGCA AAGGTTTGGT TAATTGGTGA TGGTCTAGAT TCTGCTGAAC 
    AATTCAGAGC ACAGAAAGGA ACTCTGTTTA TCCCATACTC ACAATTTCCT 
    CCAAAGATGG TACGCAAGGA CAGCTGCAGC TACTCGACAA CTCCTGCAAT 
    GGCTGTACCA AAAACGCTGC AGAATGTGCA TTCATGCGAG AATTGGCTGC 
    CAAGGAGGGT TATGAGCGCA TGGCGAATCG CGGGAATTCT TCATGCGTTG 
    GAGGGATGGA ATGAGCATGA ATGTGGTGAC AAGGTGCTTG ATATGGACAA 
    AGTTTGGTCT GCTGCAATTA TGCATGGGTT CTGCCCAGTT GCTCAAGGTT 
    GATGATTCAG TTGCAAAGTA TCAGAATAAG TTTTAGCTGG ATTATGCTAT 
    GTGGTCACCG TATCAGAAAG AGAGATGTTC TGTTGGCAAG AAGAGATATA 
    GTATCTGAGT AATTTCATCT GCCATGGTTT TTAAAACATT GTTCATCATC 
    TCCTTTGATC AAAGACTGGC CATGGCTACT TTCTGTATAA GTATCTGTCC 
    TTTTTAGTCC ATAGTGTAAT TCAGCAACAA AACACTCTTG GCATTGTTAT 
    GCAGTCTCAT GAATGTTTTT CCAGCATACG GAATTACGG

    Gene models with high sequence identity

    1. AT1G02205.2 [+] show detail - Fatty acid hydroxylase superfamily
      E-value: 0; Score: 30.40

     

    InterPro Scan Digest

    Program Description E-value
    HMMPfam Uncharacterised protein, Wax2 C-terminal 8.1e-81
    HMMPfam Fatty acid hydroxylase 7.9e-06
    HMMPanther STEROL DESATURASE 2.2e-13
    HMMPanther STEROL DESATURASE 2.2e-13
    HMMPanther STEROL DESATURASE 2.1e-66
    HMMPanther STEROL DESATURASE 2.1e-66
    HMMPanther STEROL DESATURASE 3.3e-86
    HMMPanther STEROL DESATURASE 3.3e-86

    See the detailed result >

    AT1G02205.2

    Model type
    Protein coding
    Short Description
    Fatty acid hydroxylase superfamily
    Curator Summary
    Expression of the CER1 gene associated with production of stem epicuticular wax and pollen fertility. Biochemical studies showed that cer1 mutants are blocked in the conversion of stem wax C30 aldehydes to C29 alkanes, and they also lack the secondary alcohols and ketones. These suggested the CER1 protein is an aldehyde decarbonylase, but the exact molecular function of this protein remains to be determined.
    Computational Description
    ECERIFERUM 1 (CER1); FUNCTIONS IN: aldehyde decarbonylase activity; INVOLVED IN: aldehyde catabolic process, wax biosynthetic process, cuticle development; LOCATED IN: endoplasmic reticulum; EXPRESSED IN: 18 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Fatty acid hydroxylase (InterPro:IPR006694), Uncharacterised protein, Wax2 C-terminal (InterPro:IPR021940); BEST Arabidopsis thaliana protein match is: Fatty acid hydroxylase superfamily (TAIR:AT1G02190.1); Has 2658 Blast hits to 2653 proteins in 533 species: Archae - 0; Bacteria - 823; Metazoa - 248; Fungi - 388; Plants - 579; Viruses - 3; Other Eukaryotes - 617 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR