K32335

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K32335

Phenotypes

Morphological phenotypes

Specific phenotypess were NOT observed.

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K32335

Determination of introduced cDNA(s)

  • J023118L15 - Rice Full-length cDNAs - Original: J023118L15

    full reading [+] show sequence

    Nucleotide sequence: J023118L15 - full reading
    GATATGAGAC TCTGACACGT ACGCACAGCT AGATCGAAGC CCTGGTCGAG 
    TTTATAAATT GAGATATATC CGTCCTCCAT TCTCAGGCCA GTTCTTGCAA
    GACTCTTGCC GTGTTGAGAC TGCAGAAACG AGAGCTAGCT AGCCAGAGGC
    TGCAAGAAGA TTCTGCACCC ATAGATCGTC TACCATGGCC ACAAACCCAG
    GACTCTTCAC GGAGTGGCCA TGGAAGAAGC TTGGCAGCTT CAAGTACGTG
    TTGCTGGCGC CGTGGGTAGC GCACGGGTGG TATGAGGTGG CGACCAAGGG
    GTGGCGGGAG GTGGATCTCG GGTACATCGC CATCCTCCCG TCGTTGCTGC
    TGCGGATGCT GCACAACCAA GCCTGGATCA CCATCTCCCG CCTCCAAAAC
    GCCCGTGGCC GCCGCCAGAT TGTTCGCCGC GGCATCGAGT TCGACCAGGT
    TGACCGCGAG AGGAACTGGG ACGACCAGAT CATCCTGAGC GGTATCCTAC
    TATACCTCGG CGCACTGTAC GTACCGGGCG GGCAACACTT ACCGCTGTGG
    AGGACGGATG GCGCGGGGCT GATCGCCTTG CTGCATGCGG GGCCAGTGGA
    GTTCCTCTAC TACTGGTTCC ATCGTGGCGT TGCACCACCA CTTTCTCTAC
    ACCCACTACC ACTCGCACCA CCACTCTTCA ATCGTCACTG AGCCCATCAC
    ATCCGTCATC CATCCGTTTG GCTGAGCTCG TTGCCTACGA ATTGCTCTTC
    TCGATCCCAC TGATCGCCTG TGCCTTGACT GGAACTGCTT CCATAATTGC
    ATTCGAGATG TATTTGATTT ACATTGATTT CATGAACAAC ATGGGGCACT
    GCAACTTCGA GTTGGTACCC AGCTGGCTCT TCACATGGTT CCCTCCTCTC
    AAGTATCTCA TGTACACGCC ATCGTTTCAT TCTCTGCACC ACACTCAGTT
    TCGGACAAAC TACTCCCTTT TCATGCCGTT CTACGATTAT ATCTACAACA
    CCATGGACAA GTCATCAGAC ACTCTGTATG AGAACTCACT GAAAAACAAT
    GAGGAAGAAG AAGCAGTGGA TGTGGTTCAC CTTACACACC TGACCACCCT
    GCATTCCATC TACCACATGA GGCCCGGTTT CGCCGAATTT GCGTCCAGGC
    CCTACGTTTC CAGGTGGTAC ATGAGGATGA TGTGGCCTCT GTCATGGCTC
    TCCATGGTGC TGACATGGAC GTACGGTCCT TCATTCACTG TTGAGAGGAA
    TGTCATGAAG AAGATCAGGA TGCAGTCATG GGCCATACCA AGATACAGTT
    TCCATTATGG ATTGGATTGG GAGAAGGAAG CTATCAATGA TCTTATCGAA
    AAGGCGGTAT GTGAAGCTGA CAAGAATGGG GCTAAAGTTG TAAGCCTTGG
    ACTCCTGAAT CAGGCACATA CCCTGAATAA AAGTGGAGAA CAATATCTGC
    TGAAATACCC AAAGTTGGGA GCAAGAATTG TCGATGGAAC CAGCTTAGCT
    GCTGCAGTGG TTGTCAACAG TATCCCCCAA GGCACAGACC AAGTAATTCT
    TGCAGGAAAT GTTTCCAAGG TGGCGCGTGC TGTAGCGCAA GCATTATGCA
    AGAAAAATAT CAAAGTCACG ATGACAAACA AGCAAGATTA TCATTTACTC
    AAGCCAGAAA TACCAGAAAC TGTAGCTGAC AATCTTTCGT TTTCGAAAAC
    AGGCACCGCA AAGGTTTGGT TAATTGGTGA TGGTCTAGAT TCTGCTGAAC
    AATTCAGAGC ACAGAAAGGA ACTCTGTTTA TCCCATACTC ACAATTTCCT
    CCAAAGATGG TACGCAAGGA CAGCTGCAGC TACTCGACAA CTCCTGCAAT
    GGCTGTACCA AAAACGCTGC AGAATGTGCA TTCATGCGAG AATTGGCTGC
    CAAGGAGGGT TATGAGCGCA TGGCGAATCG CGGGAATTCT TCATGCGTTG
    GAGGGATGGA ATGAGCATGA ATGTGGTGAC AAGGTGCTTG ATATGGACAA
    AGTTTGGTCT GCTGCAATTA TGCATGGGTT CTGCCCAGTT GCTCAAGGTT
    GATGATTCAG TTGCAAAGTA TCAGAATAAG TTTTAGCTGG ATTATGCTAT
    GTGGTCACCG TATCAGAAAG AGAGATGTTC TGTTGGCAAG AAGAGATATA
    GTATCTGAGT AATTTCATCT GCCATGGTTT TTAAAACATT GTTCATCATC
    TCCTTTGATC AAAGACTGGC CATGGCTACT TTCTGTATAA GTATCTGTCC
    TTTTTAGTCC ATAGTGTAAT TCAGCAACAA AACACTCTTG GCATTGTTAT
    GCAGTCTCAT GAATGTTTTT CCAGCATACG GAATTACGG

    Gene models with high sequence identity

    1. AT1G02205.2 [+] show detail - Fatty acid hydroxylase superfamily
      E-value: 0; Score: 30.40

     

    InterPro Scan Digest

    Program Description E-value
    HMMPfam Uncharacterised protein, Wax2 C-terminal 8.1e-81
    HMMPfam Fatty acid hydroxylase 7.9e-06
    HMMPanther STEROL DESATURASE 2.2e-13
    HMMPanther STEROL DESATURASE 2.2e-13
    HMMPanther STEROL DESATURASE 2.1e-66
    HMMPanther STEROL DESATURASE 2.1e-66
    HMMPanther STEROL DESATURASE 3.3e-86
    HMMPanther STEROL DESATURASE 3.3e-86

    See the detailed result >

    AT1G02205.2

    Model type
    Protein coding
    Short Description
    Fatty acid hydroxylase superfamily
    Curator Summary
    Expression of the CER1 gene associated with production of stem epicuticular wax and pollen fertility. Biochemical studies showed that cer1 mutants are blocked in the conversion of stem wax C30 aldehydes to C29 alkanes, and they also lack the secondary alcohols and ketones. These suggested the CER1 protein is an aldehyde decarbonylase, but the exact molecular function of this protein remains to be determined.
    Computational Description
    ECERIFERUM 1 (CER1); FUNCTIONS IN: aldehyde decarbonylase activity; INVOLVED IN: aldehyde catabolic process, wax biosynthetic process, cuticle development; LOCATED IN: endoplasmic reticulum; EXPRESSED IN: 18 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Fatty acid hydroxylase (InterPro:IPR006694), Uncharacterised protein, Wax2 C-terminal (InterPro:IPR021940); BEST Arabidopsis thaliana protein match is: Fatty acid hydroxylase superfamily (TAIR:AT1G02190.1); Has 2658 Blast hits to 2653 proteins in 533 species: Archae - 0; Bacteria - 823; Metazoa - 248; Fungi - 388; Plants - 579; Viruses - 3; Other Eukaryotes - 617 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR