K32514

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K32514

Phenotypes

Morphological phenotypes

Specific phenotypess were NOT observed.

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K32514

Determination of introduced cDNA(s)

  • J033132M08 - Rice Full-length cDNAs - Original: J033132M08

    full reading [+] show sequence

    Nucleotide sequence: J033132M08 - full reading
    GACACCCCCA TCCCCTCTCT CCGCGCCTCG CCTCGCCACC CGCATCGCCA 
    TCTCGCACCG CCACCTCCTC CACTCTCGGC GGCGGGGGCC TCATCGATGG
    CGTCGAGGAT GGCGCTCCGG CCCAACGACG TCACGCTCCG CCTCACCCCG
    CCCCTCGCCG CCGCCGCGCG GCGCAACCGC CGCGCCGCCG CCGGCGGTGT
    CAGGGTCTAC GCCGTCGCGT CCGGGGCCGT CTCCACCAAG GTTGAGAACA
    AGAAGCCATT TGCTCCTCCA CGAGAGGTGC ACGTCCAGGT TACACATTCC
    ATGCCACCCC AGAAGATTGA AATATTCAAG TCTCTTGATG ATTGGGCCAG
    AGATAATATT TTGTCCCACC TTAAGCCTGT CGAGAAATGT TGGCAACCAC
    AGGATTTTCT TCCTGATCCA GCCTCAGATG GGTTTCATGA TGAAGTCAAA
    GAACTTAGAG AACGTGCCAA GGAAATTCCT GATGATTATT TTGTTTGTTT
    GGTTGGAGAC ATGATTACGG AGGAAGCTCT TCCTACGTAC CAGACTATGC
    TTAACACTCT CGATGGTGTC CGAGATGAAA CAGGTGCAAG CCCCACTGCC
    TGGGCTGTTT GGACAAGGGC ATGGACTGCT GAGGAGAACA GGCATGGTGA
    CCTCCTGAAC AAATATCTCT ACCTCACTGG TAGGGTGGAC ATGAGACAAA
    TTGAGAAGAC AATTCAGTAT CTTATTGGCT CTGGAATGGA CCCTAGGACA
    GAGAACAATC CTTATCTTGG ATTCATCTAC ACCTCCTTCC AAGAGCGTGC
    GACCTTCATC TCACATGGGA ACACTGCTCG CCATGCCAAA GACTTTGGCG
    ACCTAAAACT TGCACAGATC TGTGGCATCA TCGCCTCAGA TGAGAAGCGT
    CATGAGGCTG CATACACCAA GATTGTTGAG AAGCTGTTTG AGATTGACCC
    TGATGGCACT GTGCTTGCTT TTGCTGACAT GATGAAGAAG AAGATCTCGA
    TGCCTGCCCA CCTGATGTTC GATGGGGAGG ATGATAAGCT CTTTGAGCAC
    TTCTCCATGG TTGCACAGAG GCTTGGTGTT TACACCGCCA AGGACTACGC
    CGACATCCTT GAGTTCCTCG TTAGCAGGTG GAAGATATCT GACCTGACTG
    GCCTATCTAG CGAGGGAAAC AAGGCGCAAG ACTACCTTTG CACCCTTGCT
    GCTAGGATCA GAAGGCTGGA TGAGAGGGCA CAATCGAGAG CCAAGAAAGC
    TGGTACATTG CCTTTCAGCT GGGTATATGG TAGGGAAGTT CAACTCTGAG
    CATCAGACGC CATTGCGACT TCTTCGAGCT CCAGTGTTAC TACTGTCCGT
    GCTTGTCGAG ACACATTTTG AGAACAATAC CAGGTGTGTC TTGCTACATA
    GTTCTTCAGG TTGACCAAAT GAACTGAGGG CATATGTTCG TGGTATCTTT
    GCTTAGAGTG ATAGAGAGAT TTGCGTCTGT GTTTTAGCTC TTTTTTTCTT
    TTCTGCCTTT TCATGTACAA CTTCTGGCCG TGTGGATTGG ACATGTACTG
    AACGTGAGTC TGTCGTTGGC CGTGTCAATC TGCTCGTGTG TTTAAACTGG
    CTGCTGTTCA GGTCTGAAAA TTTTGTG

    Gene models with high sequence identity

    1. AT2G43710.2 [+] show detail - Plant stearoyl-acyl-carrier-protein desaturase family protein
      E-value: 4e-171; Score: 598.00

     

    InterPro Scan Digest

    Program Description E-value
    HMMPfam Fatty acid desaturase, type 2 8.5e-185
    HMMPIR Fatty acid desaturase, type 2 1.4e-262
    superfamily Ferritin/ribonucleotide reductase-like 7.5e-163
    Gene3D Ribonucleotide reductase-related 4.9e-155

    See the detailed result >

    AT2G43710.2

    Model type
    Protein coding
    Short Description
    Plant stearoyl-acyl-carrier-protein desaturase family protein
    Curator Summary
    Encodes a stearoyl-ACP desaturase, involved in fatty acid desaturation. The ssi2 mutants have increased 18:0 and reduced 18:1 fatty acids. Exogenous application of glycerol to wild type plants mimics the ssi2 mutant phenotype. The altered 18:1 fatty acid content in the ssi2 mutants has an impact on SA- and JA-mediated defense signaling. ssi2 mutants resulted in hyper-resistance to green peach aphid and antibiosis activity in petiole exudates.
    Computational Description
    SSI2; FUNCTIONS IN: acyl-[acyl-carrier-protein] desaturase activity, stearoyl-CoA 9-desaturase activity; INVOLVED IN: in 10 processes; LOCATED IN: chloroplast, plastid; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Ribonucleotide reductase-related (InterPro:IPR012348), Ferritin/ribonucleotide reductase-like (InterPro:IPR009078), Fatty acid desaturase, type 2 (InterPro:IPR005067), Stearoyl-ACP desaturase, conserved site (InterPro:IPR005803); BEST Arabidopsis thaliana protein match is: Plant stearoyl-acyl-carrier-protein desaturase family protein (TAIR:AT3G02630.1); Has 946 Blast hits to 938 proteins in 221 species: Archae - 0; Bacteria - 436; Metazoa - 2; Fungi - 0; Plants - 450; Viruses - 0; Other Eukaryotes - 58 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR