K32722

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K32722

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T1  
whole plant (PO:0000003)
  • decreased height (PATO:0000569)
    (Adult plant::Plant height::short)
rosette leaf (PO:0000014)
  • abnormal (PATO:0000460)
    (Rosette leaf::Others)
stem (PO:0009047)
  • decreased thickness (PATO:0000592)
    (Stem::Shape::thin)
  • desaturated green (PATO:0001272)
    (Stem::Color::pale)
  • present in greater numbers in organism (PATO:0000470)
    (Stem::Number::many)
  • abnormal (PATO:0000460)
    (Stem::Others)
T2  
rosette leaf (PO:0000014)
  • epinastic (PATO:0000945)
    (Rosette leaf::Shape::epinastic)
  • desaturated green (PATO:0001272)
    (Rosette leaf::Color::pale)
  • abnormal (PATO:0000460)
    (Rosette leaf::Others)
stem (PO:0009047)
  • desaturated green (PATO:0001272)
    (Stem::Color::pale)

Invisible phenotypes

Original: K32722

Determination of introduced cDNA(s)

  • J033024P22 - Rice Full-length cDNAs - Original: J033024P22

    full reading [+] show sequence

    Nucleotide sequence: J033024P22 - full reading 
    GAGCGAGCCG AGCCGAGAGC GCAACAAAAG GAAAGCAGGG AAACCTCGCC 
    GTCCTCTCTC TCCATCCATG CCCTAGCACG CGCCGCGCAG CCTACGAGCG 
    GGAGCCACAC CTCGAGGTCT TCATCTGTTG CACTCATGAT TTAGCACCAG 
    CTGAAGTGAC CTCTGAGACT GCGCGGTGAC GTAGCCTGTT GCTAGGATAA 
    AAATCTCAGT AGTTAAGCAT GGCGGATTCT ATGGTGTCAA GTTTCTGGGG 
    GCCTGTTACT TCAACAACTG AGCTCTGCGA GGAAAATTAT GCACACTCGT 
    CATATATTGC AGAATTCTAC AATACTGTTT CTAATGTTCC ATGTGTTCTT 
    CTGGCACTTG TTGGACTTGT GAATGCTCTT CGCCAAGGTT TTGAGAAACG 
    ATTCAGTGTC CTGCACATAT CCAATATGAT ACTTGCTATC GGCAGTATGA 
    TCTTCCATGC CACCTTACAG CACGTTTTAC AGCAGAGCGA TGAGACTCCA 
    ATGGTGTGGG AGATTCTCCT ATATCTTTAT GTACTTTATT CACCAGACTG 
    GCATTACCGG AGCACTATGC CTACTTTCCT TTTCCTATAC GGTGCTGCTT 
    TTGCAGTAGT CCATTTCCTG GTGCGATTCC AAGTGGTATT CAAGTTGCAT 
    TACGTTGGCC TCTGTCTCCT ATGCATCCCA CGGATGTACA AGTACTACAT 
    ACAAACTAAA GACATGGCTG CCAAGCGTCT AGCAAAGCTG TGGGTTCTTA 
    CGTTGAGCTT GGCGACTCTT TGCTGGCTAT TTGATCGAAT GTTCTGTAAG 
    AAGCTTTCAC ATTGGTACGT CAACCCACAA GGGCACGCAT GGTGGCATAT 
    TCTCATGGGA TTTAACTCAT ACTTTGCAAA CACATTCTTA ATGTTTTGCC 
    GAGCTCAACA GCGTGGGTGG GAGCCCAAAA TTACCCACCT TTTCGGGTTC 
    TTGCCTTATG TCAAGATTCA GAAACCCCAA AAGAGGGAAT GAGTTCTCTA 
    TTTGTCAGTG CTGTTCTCCA GGACCTTAAA GCCAACGCCC TCATGTGCTT 
    AGACAAGAAG CAGAGGCATT TTTTTTGTAT ATGGGCTTCC AAGTGATTAC 
    GGACTACAGT GTTATGATGC CTCACTAGAT TTTGTTAACT TTTAACTAAC 
    GCCTGTTTTG TACCATACTT AATTGTTGCT CAGAGATTAG TTGGAGTGTT 
    GTCTCATAGT TTTGGTCATT ATTTTTTGTT TCTCTACATT TGATTTTCCG 
    TTCTAATAGG ACTGAATTTT ATTTGTTTTT TGTTTGTCTT GAATGTTGTC 
    GAACAGTTTT AGTCAAGTTA CTGCTTCATA TGGCC

    Gene models with high sequence identity

    1. AT4G22330.1 [+] show detail - Alkaline phytoceramidase (aPHC)
      E-value: 3e-110; Score: 395.00

     

    InterPro Scan Digest

    Program Description E-value
    HMMPfam Ceramidase 6.3e-65
    HMMPanther ALKALINE PHYTOCERAMIDASE (APHC) (ALKALINE CER 2.7e-80
    HMMPanther ALKALINE CERAMIDASE-RELATED 2.7e-80

    See the detailed result >

    AT4G22330.1

    Model type
    Protein coding
    Short Description
    Alkaline phytoceramidase (aPHC)
    Curator Summary
    Encodes AtCES1 for Acyl-CoA independent ceramide synthase.
    Computational Description
    ATCES1; FUNCTIONS IN: catalytic activity, hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides; INVOLVED IN: ceramide metabolic process; LOCATED IN: endoplasmic reticulum, membrane; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Ceramidase (InterPro:IPR008901); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR