K33620

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K33620

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T1  
rosette leaf (PO:0000014)
  • abnormal (PATO:0000460)
    (Rosette leaf::Others)
stem (PO:0009047)
  • abnormal (PATO:0000460)
    (Stem::Others)
T2  
rosette leaf (PO:0000014)
  • abnormal (PATO:0000460)
    (Rosette leaf::Others)
stem (PO:0009047)
  • decreased thickness (PATO:0000592)
    (Stem::Shape::thin)
  • abnormal (PATO:0000460)
    (Stem::Others)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K33620

Determination of introduced cDNA(s)

  • J023057B01 - Rice Full-length cDNAs - Original: J023057B01

    full reading [+] show sequence

    Nucleotide sequence: J023057B01 - full reading 
    GGCAAAAGTT GAGGTAATGG ATCGATCAGA GATGGGGAGG GGAAGGGTTG 
    AGCTGAAGCG CATCGAGAAC AAGATCAACC GGCAGGTCAC CTTCTCCAAG 
    CGCCGCAACG GCCTCCTCAA GAAGGCGTAC GAGCTCTCCG TGCTCTGCGA 
    CGCGGAGGTG GCGCTCATCA TCTTCTCCAG CCGTGGCAAG CTGTACGAAT 
    TCGGTAGCGC CGGAATAAAC AAGACATTGG AAAAGTACAA TAGTTGCTGT 
    TACAACGCTC AAGGTTCAAA TAGTGCTCTT GCTGGTGGTG AACATCAGAG 
    CTGGTACCAA GAGATGTCAA GGCTCAAGAC TAAGCTTGAA TGTCTCCAAC 
    GCTCTCAGAG GCACATGCTT GGTGAAGATC TTGGACCATT GAGCATAAAG 
    GAACTGCAGC AGCTGGAGAA GCAACTTGAG TACTCACTGT CACAGGCTCG 
    ACAACGAAAG ACACAAATCA TGATGGAGCA GGTCGACGAT CTTCGCCGGA 
    AGGAACGCCA GCTTGGAGAG CTCAATAAGC AACTGAAAAA CAAGCTAGAA 
    GCTGAAGCCG ATAGCAGCAA CTGCAGATCA GCCATCCAGG ATTCCTGGGT 
    CCATGGCACC GTCGTCAGTG GCGGCAGAGT GCTGAATGCT CAACCACCAC 
    CAGATATTGA CTGTGAGCCT ACTCTGCAAA TTGGGTACTA TCAATTTGTC 
    CGTCCTGAGG CGGCCAATCC AAGAAGCAAT GGAGGAGGAG GGGATCAGAA 
    CAACAACTTT GTGATGGGAT GGCCCCTCTG AAGTCCAAGC TTGCTAATAA 
    AAACGCTGGC GTGCTATAAT ATATAGTTCG GCAAATGTTG AAACACATGT 
    GTTCTCAGTA TGTATATTTC TCCCCTATCC GGTGGTACTC TTAGCACGTA 
    CCTATGAGTG TATGTTTGTA CGTTATCTAT ACTCTATGCT AGGCAACCCT 
    ATACTATTAT GGT

    Gene models with high sequence identity

    1. AT2G45650.1 [+] show detail - AGAMOUS-like 6
      E-value: 2e-70; Score: 262.00

     

    InterPro Scan Digest

    Program Description E-value
    FPrintScan Transcription factor, MADS-box 7.9e-33
    FPrintScan Transcription factor, MADS-box 7.9e-33
    FPrintScan Transcription factor, MADS-box 7.9e-33
    Coil coiled-coil 0
    Coil coiled-coil 0
    HMMPanther MADS BOX PROTEIN 1e-87
    HMMPanther MADS BOX PROTEIN 1e-87
    PatternScan Transcription factor, MADS-box 0
    ProfileScan Transcription factor, MADS-box 33.571
    ProfileScan Transcription factor, K-box 17.613
    HMMPfam Transcription factor, K-box 5.2e-30
    HMMPfam Transcription factor, MADS-box 2.3e-29
    HMMSmart Transcription factor, MADS-box 1.8e-41
    superfamily Transcription factor, MADS-box 3.7e-31

    See the detailed result >

    AT2G45650.1

    Model type
    Protein coding
    Short Description
    AGAMOUS-like 6
    Curator Summary
    sequence suggests this encodes a MADS-box transcription factor
    Computational Description
    AGAMOUS-like 6 (AGL6); FUNCTIONS IN: DNA binding, sequence-specific DNA binding transcription factor activity; INVOLVED IN: vegetative to reproductive phase transition of meristem, floral organ development; LOCATED IN: nucleus; EXPRESSED IN: 8 plant structures; EXPRESSED DURING: C globular stage, F mature embryo stage, petal differentiation and expansion stage, E expanded cotyledon stage; CONTAINS InterPro DOMAIN/s: Transcription factor, MADS-box (InterPro:IPR002100), Transcription factor, K-box (InterPro:IPR002487); BEST Arabidopsis thaliana protein match is: AGAMOUS-like 13 (TAIR:AT3G61120.1); Has 7294 Blast hits to 7293 proteins in 914 species: Archae - 0; Bacteria - 0; Metazoa - 630; Fungi - 307; Plants - 6281; Viruses - 0; Other Eukaryotes - 76 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR

  • J023002B16 - Rice Full-length cDNAs - Original: J023002B16

    full reading [+] show sequence

    Nucleotide sequence: J023002B16 - full reading 
    GTTATTAACA AACGCTTCGC CTGCCCCCAA TTCCTCTCTC TTCCTCTCAT 
    CAGTCAAGAG CGGAAGAGCT CGACACATCT CTCCGATCCC CATTTCGCCT 
    TCTGCTCCGG TGAGCTCCGA TCTGACTGCG CGTGGGGGGA ATCGATGGGG 
    TCCATGGCGG CGGCGGCGGA GGCGGCGCAG GAGGAGGAGA CGGTGGTGTT 
    CCGGTCCAAG CTCCCCGACA TCGAGATCCC CAGCCACCTC ACCCTGCAGG 
    CCTACTGCTT CGAGAAGCTT CCGGAGGTGG CCGCCCGCCC CTGCCTCATC 
    GACGGGCAGA CCGGGGCGGT GTACAGCTAC GGCGAGGTGG AGGAGCTCTC 
    CCGGCGCGCG GCGGCGGGGC TGCGGCGGCT CGGGGTGGGG AAGGGCGACG 
    TGGTGATGAG CCTCCTCCGC AACTGCCCCG AGTTCGCCTT CACCTTCCTC 
    GGCGCCGCCA GGCTGGGCGC GGCCACCACC ACCGCCAACC CGTTCTACAC 
    CCCGCACGAG ATCCACCGCC AGGCGTCGGC GGCGGGCGCC AGGGTGATCG 
    TGACCGAGGC GTGCGCGGTG GAGAAGGTGC GCGGGTTCGC CGCCGACCGC 
    GGCATCCCCG TGGTCGCCGT CGACGGGGAC TTCGACGGCT GCGTCGGGTT 
    CGGGGAGGCG ATGCTGGACG CGTCCATCGA GCCGCTCGAC GCCGACGAGG 
    AGGTCCACCC CGACGACGTC GTCGCCCTCC CTTACTCCTC CGGCACCACC 
    GGCTTGCCCA AGGGCGTCAT GCTCACCCAC CGCAGCCTCG TCACCAGCGT 
    CGCGCAGCAG GTGGACGGGG AGAACCCGAA CCTGTACTTC AGGAGGGAGG 
    ACGTGGTGCT GTGCTTGCTG CCGCTGTTCC ACATCTACTC GCTCAACTCC 
    GTGCTGCTGG CGGGGCTGCG AGCCGGGTCG GCGATCGTGA TCATGCGCAA 
    GTTCGACCTG GGCGCGCTGG TGGACCTGAC GCGGAGGCAC GGCGTCACCG 
    TCGCGCCGTT CGTGCCGCCC ATCGTGGTGG AGATCGCCAA GAGCCCCCGC 
    GTCACCGCCG ACGACCTCGC CTCCATCCGC ATGGTTATGT CCGGCGCCGC 
    CCCCATGGGG AAGGACCTCC AGGACGCCTT CATGGCCAAG ATCCCCAACG 
    CCGTCCTGGG ACAGGGGTAT GGGATGACTG AGGCTGGACC TGTGCTGGCC 
    ATGTGTCTGG CCTTCGCAAA GGAGCCATTC GAGGTCAAGT CCGGCTCGTG 
    CGGGACAGTC GTCAGAAACG CGGAGCTGAA GATCGTTGAC CCTGACACCG 
    GCGCCACCCT TGGCCGGAAC CAGTCCGGGG AGATTTGCAT CCGGGGAGAA 
    CAAATCATGA AAGGTTATCT GAATGACCCG GAGTCCACGA AGAACACCAT 
    CGACAAGGGC GGTTGGCTGC ACACAGGAGA CATTGGTTAT GTTGACGACG 
    ACGACGAGAT TTTCATTGTT GACCGGCTCA AGGAAATAAT AAAATACAAG 
    GGGTTCCAAG TACCTCCTGC GGAACTTGAA GCTCTTCTCA TCACACACCC 
    TGATATCAAG GATGCTGCCG TTGTACCGAT GATAGACGAA ATTGCGGGTG 
    AAGTGCCGGT TGCATTCATT GTACGGATTG AAGGATCTGC AATCAGCGAG 
    AATGAGATCA AGCAATTTGT GGCAAAGGAG GTTGTTTTCT ACAAGAGGCT 
    CAACAAAGTT TTCTTCGCGG ATTCAATTCC GAAGAGTCCT TCTGGCAAGA 
    TTCTCAGGAA GGACCTCAGA GCAAAGCTTG CAGCCGGCAT CCCTACCAAT 
    GATAATACAC AGTTGAAAAG CTAAATCTGA TAATATTTTT TCCTCTTTAT 
    ACACCAGTTT CAAAAATCAT GTAATATTCT TTATGAAATG GCAGAAATAC 
    GTATAGAAGG GCCATATGGT TCAATTCTTT TAGATGGCTA AGTTATACTT 
    TGTTTGTATG CCCGTTATGT GTACCGTGTC ATGTATAGAT ACCTTTTTTT 
    TTTCTTTTTG AATTTTGACA ACAAGGCTGA TTATAATGTA TACCACGTTT 
    CAATT

    Gene models with high sequence identity

    1. AT1G51680.1 [+] show detail - 4-coumarate:CoA ligase 1
      E-value: 0; Score: 644.00

     

    InterPro Scan Digest

    Program Description E-value
    Gene3D no description 4.6e-25
    Gene3D no description 7.9e-45
    Gene3D no description 2.6e-23
    Gene3D no description 2.3e-26
    HMMPfam AMP-dependent synthetase/ligase 9.3e-110
    PatternScan AMP-binding, conserved site 0
    HMMPanther 4-COUMARATE--COA LIGASE 2.3e-279
    HMMPanther ATP-DEPENDENT AMP-BINDING ENZYME FAMILY MEMBE 2.3e-279
    superfamily Acetyl-CoA synthetase-like 1.9e-158

    See the detailed result >

    AT1G51680.1

    Model type
    Protein coding
    Short Description
    4-coumarate:CoA ligase 1
    Curator Summary
    encodes an isoform of 4-coumarate:CoA ligase (4CL), which is involved in the last step of the general phenylpropanoid pathway. In addition to 4-coumarate, it also converts ferulate. The catalytic efficiency was in the following (descending) order: p-coumaric acid, ferulic acid, caffeic acid and 5-OH-ferulic acid. At4CL1 was unable to use sinapic acid as substrate.
    Computational Description
    4-coumarate:CoA ligase 1 (4CL1); CONTAINS InterPro DOMAIN/s: AMP-binding, conserved site (InterPro:IPR020845), AMP-dependent synthetase/ligase (InterPro:IPR000873); BEST Arabidopsis thaliana protein match is: 4-coumarate:CoA ligase 2 (TAIR:AT3G21240.1); Has 83980 Blast hits to 76655 proteins in 3756 species: Archae - 1185; Bacteria - 53552; Metazoa - 3476; Fungi - 4550; Plants - 2810; Viruses - 1; Other Eukaryotes - 18406 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR