K34132

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Phenotypes

Morphological phenotypes

Generation	Anatomical entity /structure development stage	Phenotypes
T1
	cauline leaf (PO:0000013)	decreased size (PATO:0000587) (Cauline leaf::Shape::small) decreased width (PATO:0000599) (Cauline leaf::Shape::narrow)
	rosette leaf (PO:0000014)	decreased width (PATO:0000599) (Rosette leaf::Shape::narrow) dark green (PATO:0001249) (Rosette leaf::Color::dark) present in greater numbers in organism (PATO:0000470) (Rosette leaf::Number::many)
	whole plant flowering stage (PO:0007016)	late (PATO:0000693) (Adult plant::Flowering::late)
	fruit (PO:0009001)	decreased length (PATO:0000574) (Silique::Shape::short)
	stem (PO:0009047)	increased thickness (PATO:0000591) (Stem::Shape::thick) abnormal (PATO:0000460) (Stem::Others)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K34132

Determination of introduced cDNA(s)

J023058F21 - Rice Full-length cDNAs - Original: J023058F21

full reading [+] show sequence

GGGGAGATGA AAAAACTCCA GCCTCATGGG CCTGTGCATA TGACCCATAG 
GCAGGCGACA CCGCGGCAGC AGAGCGCTGC ACGACCTCCG CCTCCCCCAA 
TCCGTCCCGT CTCCTCGCCC GCCGCCCTCG ACTCCCGTCT CCTCGCCTGC 
CGCTGGCGCC GATCTCCATC CTGCTAGCAG AGGTGGCGCC GGCGTCCTCC 
TGCTCCCGCC GCATTCGCTC ACCGGTGAGC ATCCTAATCC ATCCCATCCG 
GTCTCGGTCT CGGCCTCGGC CTCGAATTTC GTCTCCTCGC CCGCCGCTTG 
CGCCGATCCC CATCCTGCGA GCAGAGGTGG CGCCCGGCGT CCTCCTTCTT 
CCACCGCATC GTCGCTCGCC GCCGTTATGA ACTTCCAAAT TTGGTTCCAA 
ACTCTGAACT TTTGCTCGCC CTACTAACCC CCCTCACAAA TCGCCCAAAA 
TCCGAACGAC GCCGCCGCCG GAATCCGCCA CCGCCGCCGA CGGAAGCAAG 
GGCAGGTCAG GCCGTCGTCG GGAGGGGGGG AGAGGAGAGG CGCATGGCGA 
AGCTGGGGCC GGGGCAGGGG CTGGGGTGCG AGGCGGCGGT GGGGTTGCTG 
GCGCCAAGTA GGAAGAGGGA GTACAAGGCG TGCAACAAGC TCACCGAGGG 
GAAGCGGCCG CTCTACGCCA TCGGATTCAA CTTCCTCGAC TTCCACTACT 
ACGAGGTCTT CGCCACCGTC GGCGGCAACC GCGTGACAAC CTACAGCTGC 
CTCAAGGATG GTAATTTTGC TATCCTGCAA GCATATATTG ATGAGGATAA 
GGATGAATCG TTCTACACAC TGAGTTGGGC TTGTGATCTT GATGGCACAC 
CGCTGTTAGT GGCTGCAGGA AGCAATGGGA TCATTCGGGT CATCAACTGT 
GCCACTGAGA AGTTACTCAA GACTTTTGTT GGCCATGGCG ATTCAATAAA 
CGAGATAAGA ACTCAAGCAT TAAAGCCTTC GCTCATCATT TCTGCAAGCA 
AGGATGAATC TGTTAGGCTG TGGAATGTTC ACACAGGGAT CTGCATTTTG 
ATTTTTGCTG GAGCAGGAGG TCACCGGAAT GAAGTATTGA GTGTTGACTT 
CCACCCATCT GATATCTACC GCATAGCAAG TTGTGGCATG GATAACACTG 
TTAAAATATG GTCAATGAAG GAATTCTGGC CATATGTTGA GCAATCCTTT 
ACATGGACTG ACCTTCCATC AAAATTTCCA ACAAAATATG TGCAATTTCC 
GGTCTTGGTT GCTGTAGTAC ATTCTAACTA TGTTGATTGT ACTAGATGGC 
TTGGTGACTT CATTCTGTCA AAGAGTGTTG ACAATGAAAT TGTGCTGTGG 
GAGCCAAAAA CAAAAGAACA AAGTCCCGGG GAGGGTAGCA TTGATATTCT 
TCAGAAGTAT CCTGTGCCAG AATGTGATAT CTGGTTTATC AAATTCTCAT 
GCGATTTTCA CTTCAATCAA TTGGCAATAG GCAACCGTGA AGGAAAAGTC 
TTTGTCTGGG AAGTACAGTC CAGTCCTCCT GTTTTAACTG CTCGGCTGAC 
TAATCCGCAA TGCAAATCTG CGATAAGGCA GACTGCCGTG TCATTTGATG 
GAAGCACGAT CCTTGCCTGC AGCGAGGATG GCAGCATATG GCGATGGGAT 
GAAGTGGACC ATCCAAAAGC ATGAAAAGTA CCCTTATAGA CAGACCATGG 
CAATGCCAGA TTAAGATTGA CTTGGGAATT CCTTCATGTG TACTTTGTTG 
TGGGGGTTAT AGTAATCAGT CTTACTGTTG AAAAAAAGTG CAATCTGATA 
CTCTGAAATT AGAAGGATTG ACAGCTGAAT GCTGGGGTTA CCAACTTGAA 
TGTTGCAAAT AGGATACTGC TTCTGTTATA TGCTGAATGT TTCAAGTTAG 
GGCCTTTTTG TAAATGGGAA GATTCGGCTA TGCCAGATTT TTGGAAAAGT 
TGCCATTTGC TTTGTTACCA AAGTTGCATG GCAAAGATTG GCCC

Gene models with high sequence identity

AT3G20740.1 [+] show detail - Transducin/WD40 repeat-like superfamily protein
E-value: 2e-157; Score: 553.00

InterPro Scan Digest

Program	Description	E-value
ProfileScan	WD40 repeat 2	10.642
ProfileScan	WD40 repeat 2	11.678
ProfileScan	WD40-repeat-containing domain	19.327
PatternScan	WD40 repeat, conserved site	0
HMMPanther	FERTILIZATION-INDEPENDENT ENDOSPERM PROTEIN	1.9e-289
HMMPanther	WD-40 REPEAT PROTEIN	1.9e-289
superfamily	WD40 repeat-like-containing domain	1.8e-44
FPrintScan	G-protein beta WD-40 repeat, region	3.3e-05
FPrintScan	G-protein beta WD-40 repeat, region	3.3e-05
FPrintScan	G-protein beta WD-40 repeat, region	3.3e-05
Gene3D	WD40/YVTN repeat-like-containing domain	2.9e-43
HMMSmart	WD40 repeat	72
HMMSmart	WD40 repeat	8.8e-05
HMMSmart	WD40 repeat	6.9e-08
HMMSmart	WD40 repeat	30
HMMSmart	WD40 repeat	29
HMMSmart	WD40 repeat	0.5
HMMPfam	WD40 repeat, subgroup	0.0037
HMMPfam	WD40 repeat, subgroup	3.1e-08
HMMPfam	WD40 repeat, subgroup	2
HMMPfam	WD40 repeat, subgroup	0.4

See the detailed result >

AT3G20740.1

Model type: Protein coding
Short Description: Transducin/WD40 repeat-like superfamily protein
Curator Summary: Encodes a protein similar to the transcriptional regular of the animal Polycomb group and is involved in regulation of establishment of anterior-posterior polar axis in the endosperm and repression of flowering during vegetative phase. Mutation leads endosperm to develop in the absence of fertilization and flowers to form in seedlings and non-reproductive organs. Also exhibits maternal effect gametophytic lethal phenotype, which is suppressed by hypomethylation. Forms part of a large protein complex that can include VRN2 (VERNALIZATION 2), VIN3 (VERNALIZATION INSENSITIVE 3) and polycomb group proteins FERTILIZATION INDEPENDENT ENDOSPERM (FIE), CURLY LEAF (CLF) and SWINGER (SWN or EZA1). The complex has a role in establishing FLC (FLOWERING LOCUS C) repression during vernalization. In the ovule, the FIE transcript levels increase transiently just after fertilization.
Computational Description: FERTILIZATION-INDEPENDENT ENDOSPERM (FIE); CONTAINS InterPro DOMAIN/s: WD40 repeat 2 (InterPro:IPR019782), WD40 repeat, conserved site (InterPro:IPR019775), WD40 repeat (InterPro:IPR001680), G-protein beta WD-40 repeat, region (InterPro:IPR020472), WD40 repeat-like-containing domain (InterPro:IPR011046), WD40-repeat-containing domain (InterPro:IPR017986), WD40/YVTN repeat-like-containing domain (InterPro:IPR015943), WD40 repeat, subgroup (InterPro:IPR019781); BEST Arabidopsis thaliana protein match is: structural molecules (TAIR:AT1G79990.1); Has 16392 Blast hits to 11301 proteins in 524 species: Archae - 16; Bacteria - 2767; Metazoa - 5614; Fungi - 4038; Plants - 1850; Viruses - 0; Other Eukaryotes - 2107 (source: NCBI BLink).
Link: InterPro Scan - TAIR