K34132

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K34132

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage
Phenotypes
T1  
cauline leaf (PO:0000013)
  • decreased size (PATO:0000587)
    (Cauline leaf::Shape::small)
  • decreased width (PATO:0000599)
    (Cauline leaf::Shape::narrow)
rosette leaf (PO:0000014)
  • decreased width (PATO:0000599)
    (Rosette leaf::Shape::narrow)
  • dark green (PATO:0001249)
    (Rosette leaf::Color::dark)
  • present in greater numbers in organism (PATO:0000470)
    (Rosette leaf::Number::many)
whole plant flowering stage (PO:0007016)
  • late (PATO:0000693)
    (Adult plant::Flowering::late)
fruit (PO:0009001)
  • decreased length (PATO:0000574)
    (Silique::Shape::short)
stem (PO:0009047)
  • increased thickness (PATO:0000591)
    (Stem::Shape::thick)
  • abnormal (PATO:0000460)
    (Stem::Others)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K34132

Determination of introduced cDNA(s)

  • J023058F21 - Rice Full-length cDNAs - Original: J023058F21

    full reading [+] show sequence

    Nucleotide sequence: J023058F21 - full reading
    GGGGAGATGA AAAAACTCCA GCCTCATGGG CCTGTGCATA TGACCCATAG 
    GCAGGCGACA CCGCGGCAGC AGAGCGCTGC ACGACCTCCG CCTCCCCCAA
    TCCGTCCCGT CTCCTCGCCC GCCGCCCTCG ACTCCCGTCT CCTCGCCTGC
    CGCTGGCGCC GATCTCCATC CTGCTAGCAG AGGTGGCGCC GGCGTCCTCC
    TGCTCCCGCC GCATTCGCTC ACCGGTGAGC ATCCTAATCC ATCCCATCCG
    GTCTCGGTCT CGGCCTCGGC CTCGAATTTC GTCTCCTCGC CCGCCGCTTG
    CGCCGATCCC CATCCTGCGA GCAGAGGTGG CGCCCGGCGT CCTCCTTCTT
    CCACCGCATC GTCGCTCGCC GCCGTTATGA ACTTCCAAAT TTGGTTCCAA
    ACTCTGAACT TTTGCTCGCC CTACTAACCC CCCTCACAAA TCGCCCAAAA
    TCCGAACGAC GCCGCCGCCG GAATCCGCCA CCGCCGCCGA CGGAAGCAAG
    GGCAGGTCAG GCCGTCGTCG GGAGGGGGGG AGAGGAGAGG CGCATGGCGA
    AGCTGGGGCC GGGGCAGGGG CTGGGGTGCG AGGCGGCGGT GGGGTTGCTG
    GCGCCAAGTA GGAAGAGGGA GTACAAGGCG TGCAACAAGC TCACCGAGGG
    GAAGCGGCCG CTCTACGCCA TCGGATTCAA CTTCCTCGAC TTCCACTACT
    ACGAGGTCTT CGCCACCGTC GGCGGCAACC GCGTGACAAC CTACAGCTGC
    CTCAAGGATG GTAATTTTGC TATCCTGCAA GCATATATTG ATGAGGATAA
    GGATGAATCG TTCTACACAC TGAGTTGGGC TTGTGATCTT GATGGCACAC
    CGCTGTTAGT GGCTGCAGGA AGCAATGGGA TCATTCGGGT CATCAACTGT
    GCCACTGAGA AGTTACTCAA GACTTTTGTT GGCCATGGCG ATTCAATAAA
    CGAGATAAGA ACTCAAGCAT TAAAGCCTTC GCTCATCATT TCTGCAAGCA
    AGGATGAATC TGTTAGGCTG TGGAATGTTC ACACAGGGAT CTGCATTTTG
    ATTTTTGCTG GAGCAGGAGG TCACCGGAAT GAAGTATTGA GTGTTGACTT
    CCACCCATCT GATATCTACC GCATAGCAAG TTGTGGCATG GATAACACTG
    TTAAAATATG GTCAATGAAG GAATTCTGGC CATATGTTGA GCAATCCTTT
    ACATGGACTG ACCTTCCATC AAAATTTCCA ACAAAATATG TGCAATTTCC
    GGTCTTGGTT GCTGTAGTAC ATTCTAACTA TGTTGATTGT ACTAGATGGC
    TTGGTGACTT CATTCTGTCA AAGAGTGTTG ACAATGAAAT TGTGCTGTGG
    GAGCCAAAAA CAAAAGAACA AAGTCCCGGG GAGGGTAGCA TTGATATTCT
    TCAGAAGTAT CCTGTGCCAG AATGTGATAT CTGGTTTATC AAATTCTCAT
    GCGATTTTCA CTTCAATCAA TTGGCAATAG GCAACCGTGA AGGAAAAGTC
    TTTGTCTGGG AAGTACAGTC CAGTCCTCCT GTTTTAACTG CTCGGCTGAC
    TAATCCGCAA TGCAAATCTG CGATAAGGCA GACTGCCGTG TCATTTGATG
    GAAGCACGAT CCTTGCCTGC AGCGAGGATG GCAGCATATG GCGATGGGAT
    GAAGTGGACC ATCCAAAAGC ATGAAAAGTA CCCTTATAGA CAGACCATGG
    CAATGCCAGA TTAAGATTGA CTTGGGAATT CCTTCATGTG TACTTTGTTG
    TGGGGGTTAT AGTAATCAGT CTTACTGTTG AAAAAAAGTG CAATCTGATA
    CTCTGAAATT AGAAGGATTG ACAGCTGAAT GCTGGGGTTA CCAACTTGAA
    TGTTGCAAAT AGGATACTGC TTCTGTTATA TGCTGAATGT TTCAAGTTAG
    GGCCTTTTTG TAAATGGGAA GATTCGGCTA TGCCAGATTT TTGGAAAAGT
    TGCCATTTGC TTTGTTACCA AAGTTGCATG GCAAAGATTG GCCC

    Gene models with high sequence identity

    1. AT3G20740.1 [+] show detail - Transducin/WD40 repeat-like superfamily protein
      E-value: 2e-157; Score: 553.00

     

    InterPro Scan Digest

    Program Description E-value
    ProfileScan WD40 repeat 2 10.642
    ProfileScan WD40 repeat 2 11.678
    ProfileScan WD40-repeat-containing domain 19.327
    PatternScan WD40 repeat, conserved site 0
    HMMPanther FERTILIZATION-INDEPENDENT ENDOSPERM PROTEIN 1.9e-289
    HMMPanther WD-40 REPEAT PROTEIN 1.9e-289
    superfamily WD40 repeat-like-containing domain 1.8e-44
    FPrintScan G-protein beta WD-40 repeat, region 3.3e-05
    FPrintScan G-protein beta WD-40 repeat, region 3.3e-05
    FPrintScan G-protein beta WD-40 repeat, region 3.3e-05
    Gene3D WD40/YVTN repeat-like-containing domain 2.9e-43
    HMMSmart WD40 repeat 72
    HMMSmart WD40 repeat 8.8e-05
    HMMSmart WD40 repeat 6.9e-08
    HMMSmart WD40 repeat 30
    HMMSmart WD40 repeat 29
    HMMSmart WD40 repeat 0.5
    HMMPfam WD40 repeat, subgroup 0.0037
    HMMPfam WD40 repeat, subgroup 3.1e-08
    HMMPfam WD40 repeat, subgroup 2
    HMMPfam WD40 repeat, subgroup 0.4

    See the detailed result >

    AT3G20740.1

    Model type
    Protein coding
    Short Description
    Transducin/WD40 repeat-like superfamily protein
    Curator Summary
    Encodes a protein similar to the transcriptional regular of the animal Polycomb group and is involved in regulation of establishment of anterior-posterior polar axis in the endosperm and repression of flowering during vegetative phase. Mutation leads endosperm to develop in the absence of fertilization and flowers to form in seedlings and non-reproductive organs. Also exhibits maternal effect gametophytic lethal phenotype, which is suppressed by hypomethylation. Forms part of a large protein complex that can include VRN2 (VERNALIZATION 2), VIN3 (VERNALIZATION INSENSITIVE 3) and polycomb group proteins FERTILIZATION INDEPENDENT ENDOSPERM (FIE), CURLY LEAF (CLF) and SWINGER (SWN or EZA1). The complex has a role in establishing FLC (FLOWERING LOCUS C) repression during vernalization. In the ovule, the FIE transcript levels increase transiently just after fertilization.
    Computational Description
    FERTILIZATION-INDEPENDENT ENDOSPERM (FIE); CONTAINS InterPro DOMAIN/s: WD40 repeat 2 (InterPro:IPR019782), WD40 repeat, conserved site (InterPro:IPR019775), WD40 repeat (InterPro:IPR001680), G-protein beta WD-40 repeat, region (InterPro:IPR020472), WD40 repeat-like-containing domain (InterPro:IPR011046), WD40-repeat-containing domain (InterPro:IPR017986), WD40/YVTN repeat-like-containing domain (InterPro:IPR015943), WD40 repeat, subgroup (InterPro:IPR019781); BEST Arabidopsis thaliana protein match is: structural molecules (TAIR:AT1G79990.1); Has 16392 Blast hits to 11301 proteins in 524 species: Archae - 16; Bacteria - 2767; Metazoa - 5614; Fungi - 4038; Plants - 1850; Viruses - 0; Other Eukaryotes - 2107 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR