K34930

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Phenotypes

Morphological phenotypes

Generation	Anatomical entity /structure development stage	Phenotypes
T1
	cauline leaf (PO:0000013)	dark green (PATO:0001249) (Cauline leaf::Color::dark)
	rosette leaf (PO:0000014)	dark green (PATO:0001249) (Rosette leaf::Color::dark)
	fruit (PO:0009001)	decreased length (PATO:0000574) (Silique::Shape::short)
	seed (PO:0009010)	present in fewer numbers in organism (PATO:0001997) (Seed::Number::few)
	flower (PO:0009046)	abnormal (PATO:0000460) (Flower::Others)
	stem (PO:0009047)	abnormal (PATO:0000460) (Stem::Shape::zigzag) abnormal (PATO:0000460) (Stem::Others)
	stem internode (PO:0020142)	decreased length (PATO:0000574) (Stem::Shape::short internode)
T2	seed (PO:0009010)	increased size (PATO:0000586) (Seed::Shape::large) increased length (PATO:0000573) (Seed::Shape::long)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K34930

Determination of introduced cDNA(s)

J023107E20 - Rice Full-length cDNAs - Original: J023107E20

full reading [+] show sequence

GGTGTGAGCG CGGGAGCACC TCTCCCGACG AGCCTTCCCT TCTCCTCCTC 
GCCTTCGCGT CAGCCCGGCA TGGGGTTCCC GGTCTGCTGC TACTCCGAGC 
TGCTGCTCCC CAAGCAGCTG CTCCACCTCC TCCTGCTGCT CGGCTACATC 
CGCCGCTTCC TCCTCTGGGC GTTCCACGCC GTCGGCCTCG GGGACCTGCT 
GGATCTCGGG GACGAGCAGC AGGCGGTGCT GCAGGACCAC GCGCGCGAGC 
ACCGCGCGCC GGCGCAGGCG CTGCCGCCAC AGCAGCAGCA CCGCCGCGCC 
GAGTTCCGGA CGGTGCCGGC GATGGTCATC GAGGAGGTTC TGCCGGTGGT 
GCGGTTCGAC GAGCTGGTGG CCGCGGCCCC GGCGGTTTGC GGCGGCGGGG 
ACTGCGCGGT GTGCCTCAGC GGCATCGGCG GCGGCGACGA GGTGCGCAGG 
CTCAGCAATT GCCGCCACGT GTTCCACCGG GGCTGCCTGG ACCGCTGGAT 
GGAGCACGAC GACCAGCGCA CCTGCCCGCT CTGCCGCGCG CCGCTCATCC 
CCGACGAGAT GGCCAGCGCG CTCTGGGCCG CCGCCGGCGT GCCCGACGCC 
TCCGACTTCG ACTTCTCCTA CTTCGGCGCG CCCCTCACGC CGATGCCCAC 
GCCGACTCTG CTACGGCCGC ACGAGCTGCT GCTCACTGGC CTTGGCGGCT 
ACCAGTAACC AGTGAACCGC CGCTGTTGGT TCACGGAGTT GGCAATATCC 
ACCTACTGCA CAGCAGGGCA CCGGCGACCC GGAATGCGCA GTTTTTACAA 
CGACTGAATT GGTCGTCGTG TTCGGGGAAG ACCAAGAACA GGAGGCGCCT 
TCAACTGTAC ATAGGCAAAA ATGAGTAAAG CAAACCCCCC GAAAAAGGCT 
AGATGATTAG TAGATTTTTA TTTTTGTTTT TGTTTTTGTA CAGAAGATTC 
CATCTTCCAG GTTTGAGAAG TAGTGAATCT TTGTGCTGAT T

Gene models with high sequence identity

AT3G61460.1 [+] show detail - brassinosteroid-responsive RING-H2
E-value: 3e-24; Score: 109.00

InterPro Scan Digest

Program	Description	E-value
superfamily	RING/U-box	0.0000000000000024
HMMPanther	RING FINGER PROTEIN 11 (SID 1669) (NEDD4 WW D	0.000000000054
ProfileScan	Zinc finger, RING-type	12.622
Gene3D	no description	0.000000000022
HMMPfam	Zinc finger, C3HC4 RING-type	0.00000001
HMMSmart	Zinc finger, RING-CH-type	0.0016
HMMSmart	Zinc finger, RING-type	0.00000052

See the detailed result >

AT3G61460.1

Model type: Protein coding
Short Description: brassinosteroid-responsive RING-H2
Curator Summary: Encodes a novel ring finger protein and forms an N-terminal hydrophobic domain and a C-terminal RING-H2 signature. Expression is down regulated by brassinolide.
Computational Description: brassinosteroid-responsive RING-H2 (BRH1); FUNCTIONS IN: zinc ion binding; INVOLVED IN: response to brassinosteroid stimulus, response to chitin; LOCATED IN: endomembrane system; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Zinc finger, RING-type (InterPro:IPR001841), Zinc finger, C3HC4 RING-type (InterPro:IPR018957); BEST Arabidopsis thaliana protein match is: RING/U-box superfamily protein (TAIR:AT1G63840.1); Has 6755 Blast hits to 6739 proteins in 268 species: Archae - 0; Bacteria - 0; Metazoa - 1714; Fungi - 535; Plants - 3593; Viruses - 29; Other Eukaryotes - 884 (source: NCBI BLink).
Link: InterPro Scan - TAIR

J013058A21 - Rice Full-length cDNAs - Original: J013058A21

full reading [+] show sequence

GGGAGGCCCG TCTTCTCCCT AGTCTCTCTT CTTCCCCCTT ATCTCTAGGG 
AGGAAAGAAA AATAACAAGA ACGCACCTTT TGTCTCTCTC CTCTCCTCCG 
AGGTAAGGTT TGGGGAAGCT ACTTCTGACC ATGGCAGGCA CCCTGCTCCA 
GCCGGTTGCC CTCGGAACAA CTTTTGCAGG CCGTGTAAGT GGTCAGCGAT 
GGAAGTCTCA TGGCACAAGG AGGCCTCCCT CAATGTTGGC GATGTCCTTG 
AGCCGCCCAG TTAAGATGGC CGCATTTGTC GGTCTGAGAT CGGTGCATAG 
CTTCTCAGTC ACGCCTGTCA CCAACTTCAG ATCGACGGTT GCATCTTACA 
GATCAAGACG GGGAAGGCGT GCTCGCTTGT AACCAGATCG ATGTTTGAGA 
GGTTCACTGA GAAAGCTATT AAGGTTATAA TGCTTGCGCA AGAAGAGGCA 
AGGCGGTTGG GCCACAACTT TGTCGGTACT GAGCAAATTC TCTTGGGTCT 
GATTGGAGAA GGGACTGGTA TTGCTGCTAA GGTGCTCAAG TCAATGGGCA 
TAAATCTCAA GGATGCACGT GTGGAAGTGG AGAAGATCAT TGGGAGGGGC 
AATGGTTTCG TTGCTGTCGA AATACCATTC ACACCACGTG CAAAACGTGT 
TTTGGAGCTT TCATTGGAAG AAGCTCGTCA GCTAGGACAC AACTATATTG 
GATCTGAGCA CTTGCTTCTT GGACTGCTCC GTGAGGGTGA AGGTGTAGCA 
GCCCGTGTGC TCGAAAGCCT TGGAGCCGAT CCTAGCAATA TTCGCACGCA 
GGTTATCCGA ATGATTGGCG AGACTACAGA AGCTGTTGGT GCAGGAGTTG 
GAGGAGGGAG TAGTGGCAAT AAAATGCCAA CACTTGAGGA GTACGGAACT 
AATTTAACAA AATTAGCAGA GGAGGGAAAG CTAGATCCTG TTGTTGGAAG 
GCAACCCCAG ATTGAGCGTG TCGTACAAAT TCTGGGCAGA CGAACAAAGA 
ACAACCCATG CTTAATTGGA GAGCCTGGTG TTGGAAAGAC AGCAATTGCA 
GAAGGCCTTG CTCAACGCAT TTCTACTGGT GATGTGCCTG AAACAATTGA 
AGGAAAGAAG GTCATTACCC TTGATATGGG ACTTCTTGTT GCTGGTACAA 
AATACCGTGG AGAATTTGAA GAAAGATTAA AGAAGCTGAT GGAAGAAATC 
AAGCAGAGTG ATGAGATAAT ACTATTTATT GATGAAGTCC ACACTCTCAT 
AGGAGCAGGA GCAGCTGAGG GTGCTATTGA CGCTGCTAAC ATTTTAAAGC 
CAGCATTAGC AAGAGGAGAA CTACAGTGTA TTGGAGCCAC CACACTTGAT 
GAATACAGGA AGCATATTGA GAAAGACCCA GCATTAGAAA GACGTTTCCA 
GCCTGTAAGA GTGCCAGAGC CAACAGTTGA TGAAACCATA GAAATTCTCA 
GAGGGCTTCG GGAACGATAT GAGATCCATC ATAAACTTCG TTACACTGAT 
GATGCTCTGA TTTCAGCTGC CAAGCTATCT TATCAATACA TCAGTGATCG 
TTTCCTCCCA GATAAAGCAA TTGATTTGAT TGATGAAGCA GGTTCACGTG 
TAAGGCTTCG ACATGCCCAG GTTCCTGAAG AAGCTAGAGA GCTTGACAAG 
GAGCTCAAGC AAATCACAAA AGATAAGAAT GAAGCTGTCC GTAGCCAGGA 
CTTCGAAAAG GCTGGAGAGT TACGTGATCG TGAAATGGAA TTGAAGGCCC 
AGATAACAGC TCTCATTGAC AAGAGCAAGG AGATGAGCAA AGCAGAGACT 
GAATCAGGGG AGACAGGGCC ACTGGTCAAT GAAGCAGATA TCCAGCACAT 
TGTATCCTCG TGGACTGGTA TTCCAGTAGA GAAGGTATCA AGTGACGAGT 
CCGATAAGCT TCTTAAGATG GAAGAGACTT TGCATCAGCG TGTCATTGGT 
CAAGATGAGG CTGTGAAAGC CATAAGTCGC TCCATCCGCC GTGCTCGTGT 
GGGCCTCAAG AACCCGAACA GGCCGATTGC AAGCTTCATT TTCGCAGGTC 
CAACCGGTGT TGGTAAATCC GAGCTCGCAA AAGCACTTGC AGCATATTAC 
TTTGGATCTG AGGAGGCCAT GATCAGGCTT GATATGAGTG GATTCATGGA 
GAGGCACACT GTATCCAAGT TGATTGGTTC ACCCCCAGGG TATGTTGGGT 
ACACGGAGGG TGGACAGCTG ACTGAGGCAG TTCGACGCAG GCCATACACA 
GTCGTGCTTT TCGACGAGAT CGAAAAGGCG CATCCAGATG TATTCAACAT 
GATGCTCCAG ATCTTGGAAG ATGGAAGGCT GACTGACAGC AAGGGAAGAA 
CAGTGGACTT CAAGAACACA CTTCTCATAA TGACTTCGAA CGTCGGAAGC 
AGCGTCATCG AGAAGGGTGG TCGGAAGATA GGTTTCGATC TCGATTACGA 
TGAGAAGGAC AGCAGCTACA GCAGGATCAA GAGCCTTGTC GTCGAGGAGA 
TGAAGCAGTA CTTCCGCCCC GAGTTCCTCA ACCGTCTCGA CGAGATGATC 
GTCTTCAGGC AACTCACCAA GCTGGAGGTC AAGGAGATCG CCGAGATCAT 
GCTCAAGGAG GTCTTTGACA GGCTCAAGGC CAAGGACGTT GACCTCCAGG 
TCACCGAGAA GTTCAAGGAG CGTATCGTTG ACGAAGGCTT CAACCCGAGC 
TATGGTGCGA GGCCGCTAAG GAGGGCCATC ATGAGGCTCC TGGAGGACAG 
CCTCGCGGAG AAGATGCTAG CTGGGGAGGT GAAGGAGGGT GATTCTGCCA 
TTGTCGATGT GGATTCCGAG GGGAAGGTGA TTGTACTAAT GGCCAAAGTG 
GGTTGCCTGA GCTTTCAACT CCGGCTGTCA CTGTGTAGTA GTTCATATAT 
ACTGCAGAGT GTTAAGAGAT GCAGTGCTTT TCATTCAGAT ATATTTCTGC 
ATAGTTAGCA ACTTAGCATA ACTGTATATA TAGTATATAC AAATCAAAGG 
AGGAGGAAAC ACCAGCTGAT TCCTGGTTTC TCATGTTTGG GACACCTTTT 
TTTTTATTAA CAATAATTTC ATATGGTAGA GGGATCTT

Gene models with high sequence identity

AT5G50920.1 [+] show detail - CLPC homologue 1
E-value: 0; Score: 999.99

InterPro Scan Digest

Program	Description	E-value
Gene3D	no description	2.2e-83
Gene3D	no description	1.7e-87
Gene3D	no description	1.5e-24
FPrintScan	Chaperonin clpA/B	1.6e-41
FPrintScan	Chaperonin clpA/B	1.6e-41
FPrintScan	Chaperonin clpA/B	1.6e-41
FPrintScan	Chaperonin clpA/B	1.6e-41
HMMSmart	ATPase, AAA+ type, core	0.00000000000024
HMMSmart	ATPase, AAA+ type, core	0.00000000041
ProfileScan	UvrB/UvrC protein	11.776
superfamily	P-loop containing nucleoside triphosphate hyd	1.1e-105
superfamily	P-loop containing nucleoside triphosphate hyd	9.5e-85
superfamily	Double Clp-N motif	1.7e-36
HMMPanther	ATP-DEPENDENT CLP PROTEASE	0
HMMPanther	ATP-DEPENDENT CLP PROTEASE	0
PatternScan	Chaperonin ClpA/B, conserved site	0
Coil	coiled-coil	0
Coil	coiled-coil	0
HMMPfam	ATPase, AAA-2	1.2e-54
HMMPfam	Clp, N-terminal	8.5e-18
HMMPfam	Clp, N-terminal	2e-19
HMMPfam	ATPase, AAA-type, core	0.0000000000000021
HMMPfam	Clp ATPase, C-terminal	7.9e-25
HMMPfam	UvrB/UvrC protein	0.0000000011
HMMPfam	Protein of unknown function DUF3170	9.1e-19
HMMPfam	Protein of unknown function DUF3170	0.0000000000095

See the detailed result >

AT5G50920.1

Model type: Protein coding
Short Description: CLPC homologue 1
Curator Summary: Encodes a protein that is similar to ATP-dependent Clp protease ATP-binding subunit / ClpC. Involved in protein import into the chloroplast. May provide ATP source that drives the TIC (Translocon at the Inner envelope membrane of Chloroplasts) translocation machinery.
Computational Description: CLPC homologue 1 (CLPC1); FUNCTIONS IN: ATP-dependent peptidase activity, ATPase activity, ATP binding; INVOLVED IN: protein import into chloroplast stroma, regulation of chlorophyll biosynthetic process, protein targeting to chloroplast, chloroplast organization; LOCATED IN: in 8 components; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Clp ATPase, C-terminal (InterPro:IPR019489), ATPase, AAA-type, core (InterPro:IPR003959), ATPase, AAA-2 (InterPro:IPR013093), ATPase, AAA+ type, core (InterPro:IPR003593), Chaperonin clpA/B (InterPro:IPR001270), Chaperonin ClpA/B, conserved site (InterPro:IPR018368), UvrB/UvrC protein (InterPro:IPR001943), Clp, N-terminal (InterPro:IPR004176); BEST Arabidopsis thaliana protein match is: Clp ATPase (TAIR:AT3G48870.2); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink).
Link: InterPro Scan - TAIR