K34930

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K34930

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage
Phenotypes
T1  
cauline leaf (PO:0000013)
  • dark green (PATO:0001249)
    (Cauline leaf::Color::dark)
rosette leaf (PO:0000014)
  • dark green (PATO:0001249)
    (Rosette leaf::Color::dark)
fruit (PO:0009001)
  • decreased length (PATO:0000574)
    (Silique::Shape::short)
seed (PO:0009010)
  • present in fewer numbers in organism (PATO:0001997)
    (Seed::Number::few)
flower (PO:0009046)
  • abnormal (PATO:0000460)
    (Flower::Others)
stem (PO:0009047)
  • abnormal (PATO:0000460)
    (Stem::Shape::zigzag)
  • abnormal (PATO:0000460)
    (Stem::Others)
stem internode (PO:0020142)
  • decreased length (PATO:0000574)
    (Stem::Shape::short internode)
T2 seed (PO:0009010)
  • increased size (PATO:0000586)
    (Seed::Shape::large)
  • increased length (PATO:0000573)
    (Seed::Shape::long)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K34930

Determination of introduced cDNA(s)

  • J023107E20 - Rice Full-length cDNAs - Original: J023107E20

    full reading [+] show sequence

    Nucleotide sequence: J023107E20 - full reading
    GGTGTGAGCG CGGGAGCACC TCTCCCGACG AGCCTTCCCT TCTCCTCCTC 
    GCCTTCGCGT CAGCCCGGCA TGGGGTTCCC GGTCTGCTGC TACTCCGAGC
    TGCTGCTCCC CAAGCAGCTG CTCCACCTCC TCCTGCTGCT CGGCTACATC
    CGCCGCTTCC TCCTCTGGGC GTTCCACGCC GTCGGCCTCG GGGACCTGCT
    GGATCTCGGG GACGAGCAGC AGGCGGTGCT GCAGGACCAC GCGCGCGAGC
    ACCGCGCGCC GGCGCAGGCG CTGCCGCCAC AGCAGCAGCA CCGCCGCGCC
    GAGTTCCGGA CGGTGCCGGC GATGGTCATC GAGGAGGTTC TGCCGGTGGT
    GCGGTTCGAC GAGCTGGTGG CCGCGGCCCC GGCGGTTTGC GGCGGCGGGG
    ACTGCGCGGT GTGCCTCAGC GGCATCGGCG GCGGCGACGA GGTGCGCAGG
    CTCAGCAATT GCCGCCACGT GTTCCACCGG GGCTGCCTGG ACCGCTGGAT
    GGAGCACGAC GACCAGCGCA CCTGCCCGCT CTGCCGCGCG CCGCTCATCC
    CCGACGAGAT GGCCAGCGCG CTCTGGGCCG CCGCCGGCGT GCCCGACGCC
    TCCGACTTCG ACTTCTCCTA CTTCGGCGCG CCCCTCACGC CGATGCCCAC
    GCCGACTCTG CTACGGCCGC ACGAGCTGCT GCTCACTGGC CTTGGCGGCT
    ACCAGTAACC AGTGAACCGC CGCTGTTGGT TCACGGAGTT GGCAATATCC
    ACCTACTGCA CAGCAGGGCA CCGGCGACCC GGAATGCGCA GTTTTTACAA
    CGACTGAATT GGTCGTCGTG TTCGGGGAAG ACCAAGAACA GGAGGCGCCT
    TCAACTGTAC ATAGGCAAAA ATGAGTAAAG CAAACCCCCC GAAAAAGGCT
    AGATGATTAG TAGATTTTTA TTTTTGTTTT TGTTTTTGTA CAGAAGATTC
    CATCTTCCAG GTTTGAGAAG TAGTGAATCT TTGTGCTGAT T

    Gene models with high sequence identity

    1. AT3G61460.1 [+] show detail - brassinosteroid-responsive RING-H2
      E-value: 3e-24; Score: 109.00

     

    InterPro Scan Digest

    Program Description E-value
    superfamily RING/U-box 2.4e-15
    HMMPanther RING FINGER PROTEIN 11 (SID 1669) (NEDD4 WW D 5.4e-11
    ProfileScan Zinc finger, RING-type 12.622
    Gene3D no description 2.2e-11
    HMMPfam Zinc finger, C3HC4 RING-type 1e-08
    HMMSmart Zinc finger, RING-CH-type 0.0016
    HMMSmart Zinc finger, RING-type 5.2e-07

    See the detailed result >

    AT3G61460.1

    Model type
    Protein coding
    Short Description
    brassinosteroid-responsive RING-H2
    Curator Summary
    Encodes a novel ring finger protein and forms an N-terminal hydrophobic domain and a C-terminal RING-H2 signature. Expression is down regulated by brassinolide.
    Computational Description
    brassinosteroid-responsive RING-H2 (BRH1); FUNCTIONS IN: zinc ion binding; INVOLVED IN: response to brassinosteroid stimulus, response to chitin; LOCATED IN: endomembrane system; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Zinc finger, RING-type (InterPro:IPR001841), Zinc finger, C3HC4 RING-type (InterPro:IPR018957); BEST Arabidopsis thaliana protein match is: RING/U-box superfamily protein (TAIR:AT1G63840.1); Has 6755 Blast hits to 6739 proteins in 268 species: Archae - 0; Bacteria - 0; Metazoa - 1714; Fungi - 535; Plants - 3593; Viruses - 29; Other Eukaryotes - 884 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR
  • J013058A21 - Rice Full-length cDNAs - Original: J013058A21

    full reading [+] show sequence

    Nucleotide sequence: J013058A21 - full reading
    GGGAGGCCCG TCTTCTCCCT AGTCTCTCTT CTTCCCCCTT ATCTCTAGGG 
    AGGAAAGAAA AATAACAAGA ACGCACCTTT TGTCTCTCTC CTCTCCTCCG
    AGGTAAGGTT TGGGGAAGCT ACTTCTGACC ATGGCAGGCA CCCTGCTCCA
    GCCGGTTGCC CTCGGAACAA CTTTTGCAGG CCGTGTAAGT GGTCAGCGAT
    GGAAGTCTCA TGGCACAAGG AGGCCTCCCT CAATGTTGGC GATGTCCTTG
    AGCCGCCCAG TTAAGATGGC CGCATTTGTC GGTCTGAGAT CGGTGCATAG
    CTTCTCAGTC ACGCCTGTCA CCAACTTCAG ATCGACGGTT GCATCTTACA
    GATCAAGACG GGGAAGGCGT GCTCGCTTGT AACCAGATCG ATGTTTGAGA
    GGTTCACTGA GAAAGCTATT AAGGTTATAA TGCTTGCGCA AGAAGAGGCA
    AGGCGGTTGG GCCACAACTT TGTCGGTACT GAGCAAATTC TCTTGGGTCT
    GATTGGAGAA GGGACTGGTA TTGCTGCTAA GGTGCTCAAG TCAATGGGCA
    TAAATCTCAA GGATGCACGT GTGGAAGTGG AGAAGATCAT TGGGAGGGGC
    AATGGTTTCG TTGCTGTCGA AATACCATTC ACACCACGTG CAAAACGTGT
    TTTGGAGCTT TCATTGGAAG AAGCTCGTCA GCTAGGACAC AACTATATTG
    GATCTGAGCA CTTGCTTCTT GGACTGCTCC GTGAGGGTGA AGGTGTAGCA
    GCCCGTGTGC TCGAAAGCCT TGGAGCCGAT CCTAGCAATA TTCGCACGCA
    GGTTATCCGA ATGATTGGCG AGACTACAGA AGCTGTTGGT GCAGGAGTTG
    GAGGAGGGAG TAGTGGCAAT AAAATGCCAA CACTTGAGGA GTACGGAACT
    AATTTAACAA AATTAGCAGA GGAGGGAAAG CTAGATCCTG TTGTTGGAAG
    GCAACCCCAG ATTGAGCGTG TCGTACAAAT TCTGGGCAGA CGAACAAAGA
    ACAACCCATG CTTAATTGGA GAGCCTGGTG TTGGAAAGAC AGCAATTGCA
    GAAGGCCTTG CTCAACGCAT TTCTACTGGT GATGTGCCTG AAACAATTGA
    AGGAAAGAAG GTCATTACCC TTGATATGGG ACTTCTTGTT GCTGGTACAA
    AATACCGTGG AGAATTTGAA GAAAGATTAA AGAAGCTGAT GGAAGAAATC
    AAGCAGAGTG ATGAGATAAT ACTATTTATT GATGAAGTCC ACACTCTCAT
    AGGAGCAGGA GCAGCTGAGG GTGCTATTGA CGCTGCTAAC ATTTTAAAGC
    CAGCATTAGC AAGAGGAGAA CTACAGTGTA TTGGAGCCAC CACACTTGAT
    GAATACAGGA AGCATATTGA GAAAGACCCA GCATTAGAAA GACGTTTCCA
    GCCTGTAAGA GTGCCAGAGC CAACAGTTGA TGAAACCATA GAAATTCTCA
    GAGGGCTTCG GGAACGATAT GAGATCCATC ATAAACTTCG TTACACTGAT
    GATGCTCTGA TTTCAGCTGC CAAGCTATCT TATCAATACA TCAGTGATCG
    TTTCCTCCCA GATAAAGCAA TTGATTTGAT TGATGAAGCA GGTTCACGTG
    TAAGGCTTCG ACATGCCCAG GTTCCTGAAG AAGCTAGAGA GCTTGACAAG
    GAGCTCAAGC AAATCACAAA AGATAAGAAT GAAGCTGTCC GTAGCCAGGA
    CTTCGAAAAG GCTGGAGAGT TACGTGATCG TGAAATGGAA TTGAAGGCCC
    AGATAACAGC TCTCATTGAC AAGAGCAAGG AGATGAGCAA AGCAGAGACT
    GAATCAGGGG AGACAGGGCC ACTGGTCAAT GAAGCAGATA TCCAGCACAT
    TGTATCCTCG TGGACTGGTA TTCCAGTAGA GAAGGTATCA AGTGACGAGT
    CCGATAAGCT TCTTAAGATG GAAGAGACTT TGCATCAGCG TGTCATTGGT
    CAAGATGAGG CTGTGAAAGC CATAAGTCGC TCCATCCGCC GTGCTCGTGT
    GGGCCTCAAG AACCCGAACA GGCCGATTGC AAGCTTCATT TTCGCAGGTC
    CAACCGGTGT TGGTAAATCC GAGCTCGCAA AAGCACTTGC AGCATATTAC
    TTTGGATCTG AGGAGGCCAT GATCAGGCTT GATATGAGTG GATTCATGGA
    GAGGCACACT GTATCCAAGT TGATTGGTTC ACCCCCAGGG TATGTTGGGT
    ACACGGAGGG TGGACAGCTG ACTGAGGCAG TTCGACGCAG GCCATACACA
    GTCGTGCTTT TCGACGAGAT CGAAAAGGCG CATCCAGATG TATTCAACAT
    GATGCTCCAG ATCTTGGAAG ATGGAAGGCT GACTGACAGC AAGGGAAGAA
    CAGTGGACTT CAAGAACACA CTTCTCATAA TGACTTCGAA CGTCGGAAGC
    AGCGTCATCG AGAAGGGTGG TCGGAAGATA GGTTTCGATC TCGATTACGA
    TGAGAAGGAC AGCAGCTACA GCAGGATCAA GAGCCTTGTC GTCGAGGAGA
    TGAAGCAGTA CTTCCGCCCC GAGTTCCTCA ACCGTCTCGA CGAGATGATC
    GTCTTCAGGC AACTCACCAA GCTGGAGGTC AAGGAGATCG CCGAGATCAT
    GCTCAAGGAG GTCTTTGACA GGCTCAAGGC CAAGGACGTT GACCTCCAGG
    TCACCGAGAA GTTCAAGGAG CGTATCGTTG ACGAAGGCTT CAACCCGAGC
    TATGGTGCGA GGCCGCTAAG GAGGGCCATC ATGAGGCTCC TGGAGGACAG
    CCTCGCGGAG AAGATGCTAG CTGGGGAGGT GAAGGAGGGT GATTCTGCCA
    TTGTCGATGT GGATTCCGAG GGGAAGGTGA TTGTACTAAT GGCCAAAGTG
    GGTTGCCTGA GCTTTCAACT CCGGCTGTCA CTGTGTAGTA GTTCATATAT
    ACTGCAGAGT GTTAAGAGAT GCAGTGCTTT TCATTCAGAT ATATTTCTGC
    ATAGTTAGCA ACTTAGCATA ACTGTATATA TAGTATATAC AAATCAAAGG
    AGGAGGAAAC ACCAGCTGAT TCCTGGTTTC TCATGTTTGG GACACCTTTT
    TTTTTATTAA CAATAATTTC ATATGGTAGA GGGATCTT

    Gene models with high sequence identity

    1. AT5G50920.1 [+] show detail - CLPC homologue 1
      E-value: 0; Score: 999.99

     

    InterPro Scan Digest

    Program Description E-value
    Gene3D no description 2.2e-83
    Gene3D no description 1.7e-87
    Gene3D no description 1.5e-24
    FPrintScan Chaperonin clpA/B 1.6e-41
    FPrintScan Chaperonin clpA/B 1.6e-41
    FPrintScan Chaperonin clpA/B 1.6e-41
    FPrintScan Chaperonin clpA/B 1.6e-41
    HMMSmart ATPase, AAA+ type, core 2.4e-13
    HMMSmart ATPase, AAA+ type, core 4.1e-10
    ProfileScan UvrB/UvrC protein 11.776
    superfamily P-loop containing nucleoside triphosphate hyd 1.1e-105
    superfamily P-loop containing nucleoside triphosphate hyd 9.5e-85
    superfamily Double Clp-N motif 1.7e-36
    HMMPanther ATP-DEPENDENT CLP PROTEASE 0
    HMMPanther ATP-DEPENDENT CLP PROTEASE 0
    PatternScan Chaperonin ClpA/B, conserved site 0
    Coil coiled-coil 0
    Coil coiled-coil 0
    HMMPfam ATPase, AAA-2 1.2e-54
    HMMPfam Clp, N-terminal 8.5e-18
    HMMPfam Clp, N-terminal 2e-19
    HMMPfam ATPase, AAA-type, core 2.1e-15
    HMMPfam Clp ATPase, C-terminal 7.9e-25
    HMMPfam UvrB/UvrC protein 1.1e-09
    HMMPfam Protein of unknown function DUF3170 9.1e-19
    HMMPfam Protein of unknown function DUF3170 9.5e-12

    See the detailed result >

    AT5G50920.1

    Model type
    Protein coding
    Short Description
    CLPC homologue 1
    Curator Summary
    Encodes a protein that is similar to ATP-dependent Clp protease ATP-binding subunit / ClpC. Involved in protein import into the chloroplast. May provide ATP source that drives the TIC (Translocon at the Inner envelope membrane of Chloroplasts) translocation machinery.
    Computational Description
    CLPC homologue 1 (CLPC1); FUNCTIONS IN: ATP-dependent peptidase activity, ATPase activity, ATP binding; INVOLVED IN: protein import into chloroplast stroma, regulation of chlorophyll biosynthetic process, protein targeting to chloroplast, chloroplast organization; LOCATED IN: in 8 components; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Clp ATPase, C-terminal (InterPro:IPR019489), ATPase, AAA-type, core (InterPro:IPR003959), ATPase, AAA-2 (InterPro:IPR013093), ATPase, AAA+ type, core (InterPro:IPR003593), Chaperonin clpA/B (InterPro:IPR001270), Chaperonin ClpA/B, conserved site (InterPro:IPR018368), UvrB/UvrC protein (InterPro:IPR001943), Clp, N-terminal (InterPro:IPR004176); BEST Arabidopsis thaliana protein match is: Clp ATPase (TAIR:AT3G48870.2); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR