K35426

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K35426

Phenotypes

Morphological phenotypes

Specific phenotypess were NOT observed.

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K35426

Determination of introduced cDNA(s)

  • J023036P12 - Rice Full-length cDNAs - Original: J023036P12

    full reading [+] show sequence

    Nucleotide sequence: J023036P12 - full reading
    GAGAAGCCCA AAACACACGA AGCAAAGCTG CCTCGTCTGT GTCACTGACA 
    GATGGGGTCC ATGGAGCAGC AGCAGCCGGA GTCGGCGGCG CCGGCGACGG
    AGGCGTCGCC GGAGATCATC TTCCGGTCGA AGCTGCAAGA CATCGCCATC
    ACCAACACCC TCCCGCTCCA CCGCTACTGC TTCGAGCGGC TCCCGGAGGT
    GGCGGCGCGC CCATGCCTCA TCGACGGAGC CACCGGCGGC GTGCTCACCT
    ACGCCGACGT CGACCGCCTG TCCCGCCGCC TCGCCGCCGC GCTCCGCCGC
    GCGCCGCTGG GGCTCCGGCG GGGCGGCGTC GTGATGAGCC TGCTGCGGAA
    CTCGCCGGAG TTCGTGCTGT CCTTCTTCGC CGCGTCGCGC GTCGGCGCCG
    CGGTGACCAC GGCGAACCCA ATGTCCACCC CGCACGAGAT CGAGAGCCAG
    CTCGCCGCCG CCGGCGCCAC CGTCGTCATC ACCGAGTCCA TGGCCGCCGA
    CAAGCTCCCC TCCCACTCCC ACGGCGCCCT CACCGTCGTC CTCATCGACG
    AGCGCCGCGA CGGCTGCCTC CACTTCTGGG ACGACCTCAT GTCGGAGGAC
    GAAGCGTCGC CGCTCGCCGG CGACGAGGAC GACGAGAAGG TGTTCGACCC
    GGACGACGTG GTGGCGCTGC CGTACTCGTC GGGGACGACG GGTCTCCCCA
    AGGGGGTGAT GCTAACGCAC CGGAGCCTGA GCACGAGCGT GGCGCAGCAG
    GTCGACGGCG AGAACCCAAA CATCGGGCTC CACGCCGGCG ACGTGATCCT
    GTGCGCGCTG CCCATGTTCC ACATCTACTC GCTCAACACC ATCATGATGT
    GCGGCCTCCG CGTCGGCGCC GCCATCGTCG TCATGCGCCG CTTCGACCTC
    GCCGCCATGA TGGACCTCGT CGAGCGCCAC CGCGTCACCA TCGCGCCGCT
    CGTGCCGCCC ATCGTCGTGG CCGTGGCCAA GAGCGAGGCG GCGGCGGCGC
    GAGACCTCTC GTCGGTGCGG ATGGTGCTCT CCGGCGCGGC GCCCATGGGC
    AAGGACATCG AGGACGCGTT CATGGCCAAG CTCCCCGGCG CCGTGCTCGG
    ACAGGGGTAT GGAATGACTG AGGCAGGGCC AGTGCTGTCA ATGTGCCTAG
    CATTCGCCAA GGAGCCCTTC AAGGTGAAGT CCGGTGCGTG CGGGACGGTG
    GTGCGCAACG CCGAGCTGAA GATCATCGAC CCCGACACCG GGAAGTCGCT
    TGGCCGGAAC CTGCCCGGCG AGATCTGCAT CAGGGGGCAA CAGATCATGA
    AAGGTTACCT GAACAACCCA GAGGCCACGA AGAACACCAT TGATGCAGAA
    GGGTGGCTGC ACACCGGCGA CATCGGCTAC GTCGACGACG ACGACGAGAT
    CTTCATCGTG GATAGGCTCA AGGAGATCAT CAAATACAGG GGCTTCCAGG
    TAGCCCCTGC AGAGCTGGAG GCCCTTCTCA TCACCCATCC AAGCATTGCT
    GATGCTGCTG TTGTTGGGAA ACAAATTGAG CCTGAAATCG GAGAAATACC
    AGTCGCCTTC GTGGCGAAAA CCGAAGGGAG TGAGCTGAGT GAAGATGATG
    TCAAACAATT CGTAGCAAAG GAGGTGATCT ACTACAAGAA GATCCGGGAG
    GTCTTCTTCG TTGACAAAAT CCCCAAGGCG CCGTCAGGGA AGATCCTGAG
    AAAGGAACTG AGGAAGCAGC TGCAACATCT TCAACAAGAA GCTTAATTTA
    CAACCGGCCT CAGACATGAA AAGAATTTAG CATGAACAAG CAAGTTCCAT
    GATTCTAATG TTGCAAATTA CACATTCTGT TCTGAATAGT TTGTGTCTTC
    CATTTATATG TGATGGGGGA AAAAAACCCA GGTACAAATG GATTCTGTGC
    AATACAAAGG GTGGTGTTAC CGTGTTAGGT CGGCTAGGTT TGCTCATTTG
    CAGAGAGACA GGACACTGGG GAAGCCATGT ACAAAAATGT TCAGACCTGT
    TTATTGTTCT TGCTGGTGTC TTTGTAATAT TTTGTTTAAA CATTTATATC
    ATTCATTATT ATTGTT

    Gene models with high sequence identity

    1. AT1G51680.1 [+] show detail - 4-coumarate:CoA ligase 1
      E-value: 2e-170; Score: 596.00

     

    InterPro Scan Digest

    Program Description E-value
    HMMPanther 4-COUMARATE--COA LIGASE 5.7e-277
    HMMPanther ATP-DEPENDENT AMP-BINDING ENZYME FAMILY MEMBE 5.7e-277
    HMMPfam AMP-dependent synthetase/ligase 3.2e-112
    PatternScan AMP-binding, conserved site 0
    Gene3D no description 2.2e-23
    Gene3D no description 1.5e-47
    Gene3D no description 6.5e-23
    Gene3D no description 1.2e-24
    superfamily Acetyl-CoA synthetase-like 2.9e-158

    See the detailed result >

    AT1G51680.1

    Model type
    Protein coding
    Short Description
    4-coumarate:CoA ligase 1
    Curator Summary
    encodes an isoform of 4-coumarate:CoA ligase (4CL), which is involved in the last step of the general phenylpropanoid pathway. In addition to 4-coumarate, it also converts ferulate. The catalytic efficiency was in the following (descending) order: p-coumaric acid, ferulic acid, caffeic acid and 5-OH-ferulic acid. At4CL1 was unable to use sinapic acid as substrate.
    Computational Description
    4-coumarate:CoA ligase 1 (4CL1); CONTAINS InterPro DOMAIN/s: AMP-binding, conserved site (InterPro:IPR020845), AMP-dependent synthetase/ligase (InterPro:IPR000873); BEST Arabidopsis thaliana protein match is: 4-coumarate:CoA ligase 2 (TAIR:AT3G21240.1); Has 83980 Blast hits to 76655 proteins in 3756 species: Archae - 1185; Bacteria - 53552; Metazoa - 3476; Fungi - 4550; Plants - 2810; Viruses - 1; Other Eukaryotes - 18406 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR