K35426

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K35426

Phenotypes

Morphological phenotypes

Specific phenotypess were NOT observed.

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K35426

Determination of introduced cDNA(s)

  • J023036P12 - Rice Full-length cDNAs - Original: J023036P12

    full reading [+] show sequence

    Nucleotide sequence: J023036P12 - full reading 
    GAGAAGCCCA AAACACACGA AGCAAAGCTG CCTCGTCTGT GTCACTGACA 
    GATGGGGTCC ATGGAGCAGC AGCAGCCGGA GTCGGCGGCG CCGGCGACGG 
    AGGCGTCGCC GGAGATCATC TTCCGGTCGA AGCTGCAAGA CATCGCCATC 
    ACCAACACCC TCCCGCTCCA CCGCTACTGC TTCGAGCGGC TCCCGGAGGT 
    GGCGGCGCGC CCATGCCTCA TCGACGGAGC CACCGGCGGC GTGCTCACCT 
    ACGCCGACGT CGACCGCCTG TCCCGCCGCC TCGCCGCCGC GCTCCGCCGC 
    GCGCCGCTGG GGCTCCGGCG GGGCGGCGTC GTGATGAGCC TGCTGCGGAA 
    CTCGCCGGAG TTCGTGCTGT CCTTCTTCGC CGCGTCGCGC GTCGGCGCCG 
    CGGTGACCAC GGCGAACCCA ATGTCCACCC CGCACGAGAT CGAGAGCCAG 
    CTCGCCGCCG CCGGCGCCAC CGTCGTCATC ACCGAGTCCA TGGCCGCCGA 
    CAAGCTCCCC TCCCACTCCC ACGGCGCCCT CACCGTCGTC CTCATCGACG 
    AGCGCCGCGA CGGCTGCCTC CACTTCTGGG ACGACCTCAT GTCGGAGGAC 
    GAAGCGTCGC CGCTCGCCGG CGACGAGGAC GACGAGAAGG TGTTCGACCC 
    GGACGACGTG GTGGCGCTGC CGTACTCGTC GGGGACGACG GGTCTCCCCA 
    AGGGGGTGAT GCTAACGCAC CGGAGCCTGA GCACGAGCGT GGCGCAGCAG 
    GTCGACGGCG AGAACCCAAA CATCGGGCTC CACGCCGGCG ACGTGATCCT 
    GTGCGCGCTG CCCATGTTCC ACATCTACTC GCTCAACACC ATCATGATGT 
    GCGGCCTCCG CGTCGGCGCC GCCATCGTCG TCATGCGCCG CTTCGACCTC 
    GCCGCCATGA TGGACCTCGT CGAGCGCCAC CGCGTCACCA TCGCGCCGCT 
    CGTGCCGCCC ATCGTCGTGG CCGTGGCCAA GAGCGAGGCG GCGGCGGCGC 
    GAGACCTCTC GTCGGTGCGG ATGGTGCTCT CCGGCGCGGC GCCCATGGGC 
    AAGGACATCG AGGACGCGTT CATGGCCAAG CTCCCCGGCG CCGTGCTCGG 
    ACAGGGGTAT GGAATGACTG AGGCAGGGCC AGTGCTGTCA ATGTGCCTAG 
    CATTCGCCAA GGAGCCCTTC AAGGTGAAGT CCGGTGCGTG CGGGACGGTG 
    GTGCGCAACG CCGAGCTGAA GATCATCGAC CCCGACACCG GGAAGTCGCT 
    TGGCCGGAAC CTGCCCGGCG AGATCTGCAT CAGGGGGCAA CAGATCATGA 
    AAGGTTACCT GAACAACCCA GAGGCCACGA AGAACACCAT TGATGCAGAA 
    GGGTGGCTGC ACACCGGCGA CATCGGCTAC GTCGACGACG ACGACGAGAT 
    CTTCATCGTG GATAGGCTCA AGGAGATCAT CAAATACAGG GGCTTCCAGG 
    TAGCCCCTGC AGAGCTGGAG GCCCTTCTCA TCACCCATCC AAGCATTGCT 
    GATGCTGCTG TTGTTGGGAA ACAAATTGAG CCTGAAATCG GAGAAATACC 
    AGTCGCCTTC GTGGCGAAAA CCGAAGGGAG TGAGCTGAGT GAAGATGATG 
    TCAAACAATT CGTAGCAAAG GAGGTGATCT ACTACAAGAA GATCCGGGAG 
    GTCTTCTTCG TTGACAAAAT CCCCAAGGCG CCGTCAGGGA AGATCCTGAG 
    AAAGGAACTG AGGAAGCAGC TGCAACATCT TCAACAAGAA GCTTAATTTA 
    CAACCGGCCT CAGACATGAA AAGAATTTAG CATGAACAAG CAAGTTCCAT 
    GATTCTAATG TTGCAAATTA CACATTCTGT TCTGAATAGT TTGTGTCTTC 
    CATTTATATG TGATGGGGGA AAAAAACCCA GGTACAAATG GATTCTGTGC 
    AATACAAAGG GTGGTGTTAC CGTGTTAGGT CGGCTAGGTT TGCTCATTTG 
    CAGAGAGACA GGACACTGGG GAAGCCATGT ACAAAAATGT TCAGACCTGT 
    TTATTGTTCT TGCTGGTGTC TTTGTAATAT TTTGTTTAAA CATTTATATC 
    ATTCATTATT ATTGTT

    Gene models with high sequence identity

    1. AT1G51680.1 [+] show detail - 4-coumarate:CoA ligase 1
      E-value: 2e-170; Score: 596.00

     

    InterPro Scan Digest

    Program Description E-value
    HMMPanther 4-COUMARATE--COA LIGASE 5.7e-277
    HMMPanther ATP-DEPENDENT AMP-BINDING ENZYME FAMILY MEMBE 5.7e-277
    HMMPfam AMP-dependent synthetase/ligase 3.2e-112
    PatternScan AMP-binding, conserved site 0
    Gene3D no description 2.2e-23
    Gene3D no description 1.5e-47
    Gene3D no description 6.5e-23
    Gene3D no description 1.2e-24
    superfamily Acetyl-CoA synthetase-like 2.9e-158

    See the detailed result >

    AT1G51680.1

    Model type
    Protein coding
    Short Description
    4-coumarate:CoA ligase 1
    Curator Summary
    encodes an isoform of 4-coumarate:CoA ligase (4CL), which is involved in the last step of the general phenylpropanoid pathway. In addition to 4-coumarate, it also converts ferulate. The catalytic efficiency was in the following (descending) order: p-coumaric acid, ferulic acid, caffeic acid and 5-OH-ferulic acid. At4CL1 was unable to use sinapic acid as substrate.
    Computational Description
    4-coumarate:CoA ligase 1 (4CL1); CONTAINS InterPro DOMAIN/s: AMP-binding, conserved site (InterPro:IPR020845), AMP-dependent synthetase/ligase (InterPro:IPR000873); BEST Arabidopsis thaliana protein match is: 4-coumarate:CoA ligase 2 (TAIR:AT3G21240.1); Has 83980 Blast hits to 76655 proteins in 3756 species: Archae - 1185; Bacteria - 53552; Metazoa - 3476; Fungi - 4550; Plants - 2810; Viruses - 1; Other Eukaryotes - 18406 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR