K35524

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K35524

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T1  
cauline leaf (PO:0000013)
  • abnormal (PATO:0000460)
    (Cauline leaf::Others)
rosette leaf (PO:0000014)
  • decreased size (PATO:0000587)
    (Rosette leaf::Shape::small)
  • abnormal (PATO:0000460)
    (Rosette leaf::Others)
seed (PO:0009010)
  • present in fewer numbers in organism (PATO:0001997)
    (Seed::Number::few)
stem (PO:0009047)
  • decreased thickness (PATO:0000592)
    (Stem::Shape::thin)
  • abnormal (PATO:0000460)
    (Stem::Shape::zigzag)
  • abnormal (PATO:0000460)
    (Stem::Others)
T2 seed (PO:0009010)
  • increased size (PATO:0000586)
    (Seed::Shape::large)
  • increased length (PATO:0000573)
    (Seed::Shape::long)
  • decreased width (PATO:0000599)
    (Seed::Shape::narrow)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K35524

Determination of introduced cDNA(s)

  • J033052B21 - Rice Full-length cDNAs - Original: J033052B21

    full reading [+] show sequence

    Nucleotide sequence: J033052B21 - full reading 
    GTCTTTTCTT TCTAGGGTTT CGTCTCCCCC AATGGCGGCG GCCACCACGT 
    CGCGGCGCGG CCCGGGCGCC ATGGACGACG AGAACCTCAC CTTCGAGACC 
    TCCCCGGGGG TCGAGGTCAT CAGCAGCTTC GACCAGATGG GGATCCGCGA 
    CGACCTCCTC CGCGGCATCT ACGCCTACGG CTTCGAGAAG CCCTCCGCCA 
    TCCAGCAGCG CGCCGTCCTC CCCATCATCA GCGGCCGCGA CGTCATCGCC 
    CAGGCCCAGT CCGGGACCGG CAAGACCTCC ATGATCTCGC TCTCCGTCTG 
    CCAGATCGTA GACACCGCCG TCCGTGAGGT GCAGGCTTTA ATACTGTCAC 
    CAACTAGAGA ACTTGCTGCA CAAACAGAAA GAGTTATGCT GGCTATCGGT 
    GACTTCATCA ATATCCAAGT GCATGCTTGT ATTGGTGGCA AAAGTATTGG 
    TGAGGATATT AGAAAGCTTG AGCACGGAGT GCATGTGGTG TCAGGAACAC 
    CTGGCAGAGT CTGTGATATG ATCAAGAGAA GGACCTTGCG TACAAGAGCC 
    ATTAAGCTCC TAATTCTGGA TGAAGCTGAT GAGATGTTGG GCAGAGGCTT 
    TAAGGATCAG ATATATGATG TGTACAGATA CCTCCCTCCA GAACTCCAGG 
    TTTGCTTGAT CTCCGCAACT CTGCCTCACG AGATCTTGGA AATGACCAGC 
    AAGTTCATGA CTGATCCAGT TCGGATCCTT GTGAAGCGTG ATGAATTGAC 
    TCTAGAGGGC ATCAAACAAT TCTTTGTTGC TGTTGAGAAA GAAGAATGGA 
    AGTTTGACAC GCTTTGTGAT CTTTATGATA CACTGACAAT CACCCAAGCT 
    GTCATTTTCT GCAACACAAA GAGAAAGGTT GATTGGCTTA CGGAAAGAAT 
    GCGCAGCAAT AACTTCACAG TATCAGCTAT GCATGGCGAC ATGCCTCAAA 
    AGGAAAGGGA TGCCATTATG GGTGAATTCA GGTCTGGTGC AACCCGTGTT 
    CTAATCACGA CAGATGTGTG GGCTCGAGGC CTCGATGTTC AGCAGGTCTC 
    TCTTGTCATA AATTATGATC TCCCAAATAA TCGTGAACTT TACATCCATC 
    GCATTGGTCG CTCTGGACGT TTTGGTCGCA AGGGTGTGGC CATCAATTTT 
    GTCAAAAAGG AAGACATCCG TATCCTGAGA GATATCGAGC AGTACTACAG 
    CACGCAGATT GATGAAATGC CAATGAATGT TGCTGATCTA ATTTGAGTGC 
    TGGAGTGGCG CTACCATGTT TGATCATCTG GTCTGGCAAC AACTGAGCTT 
    GTTTGTTGTG ATTTTGGTTG ACATGCTTTG CCGTGACTGT ATTGCTATGG 
    ACTTTAAAGG GTTGCGAGTC TAATACTAGT GATTGGTTTC GTTCCCCTCT 
    CTTGTGACAT GATCTGTATA TAATTTTAGT GCCAGCTCAT GTAACACTAT 
    GATTGCATTT CTGCTCAGAA CATGAGACTT GTTCGTTATA TTTATTGCCA 
    CAAGGATTTG ATG

    Gene models with high sequence identity

    1. AT3G19760.1 [+] show detail - eukaryotic initiation factor 4A-III
      E-value: 0; Score: 699.00

     

    InterPro Scan Digest

    Program Description E-value
    HMMSmart DEAD-like helicase, N-terminal 1.6e-54
    HMMSmart DNA/RNA helicase, C-terminal 3.6e-34
    HMMPanther EUKARYOTIC INITIATION FACTOR 4A 1.6e-297
    HMMPanther DEAD BOX ATP-DEPENDENT RNA HELICASE 1.6e-297
    PatternScan RNA helicase, ATP-dependent, DEAD-box, conserved site 0
    HMMPfam DNA/RNA helicase, DEAD/DEAH box type, N-terminal 1.8e-42
    HMMPfam DNA/RNA helicase, C-terminal 1e-26
    superfamily P-loop containing nucleoside triphosphate hyd 2e-56
    superfamily P-loop containing nucleoside triphosphate hyd 6.4e-50
    Gene3D no description 4.9e-65
    Gene3D no description 4.3e-54
    ProfileScan Helicase, superfamily 1/2, ATP-binding domain 25.756
    ProfileScan DNA/RNA helicase, C-terminal 27.225
    ProfileScan RNA helicase, DEAD-box type, Q motif 10.793

    See the detailed result >

    AT3G19760.1

    Model type
    Protein coding
    Short Description
    eukaryotic initiation factor 4A-III
    Curator Summary
    Encodes an RNA helicase that may be a component of the Exon Junction Complex. Subcellular localization is modulated by stress. Under normal conditions it is localized to the nuceloplasm but under hyopoxic conditions it localizes to the nucleolus and splicing speckles.
    Computational Description
    eukaryotic initiation factor 4A-III (EIF4A-III); FUNCTIONS IN: protein binding, ATP-dependent helicase activity; INVOLVED IN: mRNA processing, response to hypoxia; LOCATED IN: in 6 components; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 16 growth stages; CONTAINS InterPro DOMAIN/s: RNA helicase, DEAD-box type, Q motif (InterPro:IPR014014), DNA/RNA helicase, DEAD/DEAH box type, N-terminal (InterPro:IPR011545), DEAD-like helicase, N-terminal (InterPro:IPR014001), DNA/RNA helicase, C-terminal (InterPro:IPR001650), Helicase, superfamily 1/2, ATP-binding domain (InterPro:IPR014021); BEST Arabidopsis thaliana protein match is: DEA(D/H)-box RNA helicase family protein (TAIR:AT1G51380.1); Has 50040 Blast hits to 49280 proteins in 3163 species: Archae - 836; Bacteria - 27183; Metazoa - 6400; Fungi - 4843; Plants - 2693; Viruses - 42; Other Eukaryotes - 8043 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR