K35645

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K35645

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T2 seed (PO:0009010)
  • decreased size (PATO:0000587)
    (Seed::Shape::small)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K35645

Determination of introduced cDNA(s)

  • J013045K17 - Rice Full-length cDNAs - Original: J013045K17

    full reading [+] show sequence

    Nucleotide sequence: J013045K17 - full reading 
    GAACTCCACG AGCCGCCATT TCCCAAGGAG GAGCAAGGAT TGTTGTTGTT 
    TCGCTGTCAG ATCGATTCCT GACGGGATAA TGGGTGAGAC CATCGCCAAG 
    GATGTCACCG AGTTGATTGG GAACACGCCG TTGGTGTACC TCAACCGGGT 
    GACGGATGGG TGCGTCGGGC GCGTCGCGGC CAAGCTCGAG TCCATGGAGC 
    CATGCTCCAG CGTCAAGGAT AGGATTGGAT ACAGTATGAT CACTGATGCA 
    GAGGAGAAGG GGCTGATCAC TCCAGGCAAG AGTGTGCTGA TTGAGCCAAC 
    TAGTGGCAAC ACAGGCATTG GACTGGCCTT CATGGCTGCT GCAAAGGGTT 
    ACAGGCTTGT ACTCACGATG CCGGCCTCCA TGAGCATGGA GAGGAGAATC 
    ATATTGAAGG CTTTTGGTGC TGAATTGATA CTTACTGACC CACTCTTGGG 
    AATGAAAGGA GCTGTCCAAA AGGCAGAAGA ACTGGCAGCG AAGACAAACA 
    ACTCATTTAT CCTCCAACAA TTCGAGAACC CTGCTAACCC AAAGATCCAT 
    TACGAGACCA CTGGACCTGA AATCTGGAAA GGAACAGGAG GTAAAGTTGA 
    TGGTTTAGTT TCTGGTATTG GGACAGGTGG CACTATTACT GGAGCTGGAC 
    GATACCTCAG AGAGCAAAAT CCTGATATCA AGATCTATGG TGTGGAGCCA 
    GTCGAGAGCG CTGTCTTATC TGGTGGAAAG CCTGGGCCAC ACAAGATTCA 
    AGGAATTGGA GCTGGTTTTG TTCCTGGGGT CCTGGATGTT GACCTCATCA 
    ATGAAACTGT ACAAGTTTCA AGTGATGAAG CTATCGAGAT GGCAAAGGCT 
    CTTGCATTGA AAGAAGGGTT GCTGGTTGGA ATATCTTCAG GTGCAGCTGC 
    AGCAGCAGCT GTTAGGCTCG CTCAGAGGCC GGAAAATGAA GGAAAACTTT 
    TTGTTGTTGT CTTCCCAAGC TTTGGTGAGC GGTACCTTTC GTCGGTGCTC 
    TTCCAGTCCA TCAAGAAGGA AGCTGAAAAC ATGGTGGTTG AATGAAATGC 
    ACAATATCCG GGAATCCACA GGAATAAAAG TTTGGTATCT CTGCTTGTGT 
    GATTAAACAT ACATTGTCCT GCCATTTTCA AGTTGTTCTG CTTGTGTAGC 
    AATGGGGAAC ACAGTGTGTT AGCCTTGTAG TGTTAAACAG TTTACATTTT 
    ATCTTCCCTG TATATCAGAA CCCTTTACAT GGGCATTTGT CAGCCAGTGT 
    GAATGAAATA AAGCATCATA TGATTTGTCT C

    Gene models with high sequence identity

    1. AT4G14880.4 [+] show detail - O-acetylserine (thiol) lyase (OAS-TL) isoform A1
      E-value: 3e-138; Score: 488.00

     

    InterPro Scan Digest

    Program Description E-value
    PatternScan Cysteine synthase/cystathionine beta-synthase P-phosphate-binding site 0
    HMMPanther CYSTEINE SYNTHASE 5.6e-150
    HMMPanther SER/THR DEHYDRATASE, TRP SYNTHASE 5.6e-150
    Gene3D no description 5.2e-68
    HMMTigr Cysteine synthase K/M 1.4e-212
    HMMTigr Cysteine synthase A 1.1e-216
    HMMPfam Pyridoxal phosphate-dependent enzyme, beta subunit 1.7e-58
    superfamily Pyridoxal phosphate-dependent enzyme, beta subunit 4e-92

    See the detailed result >

    AT4G14880.4

    Model type
    Protein coding
    Short Description
    O-acetylserine (thiol) lyase (OAS-TL) isoform A1
    Curator Summary
    Encodes a cytosolic isoform of cytosolic O-acetylserine(thiol)lyase, a key enzyme in cysteine biosynthesis and for the fixation of inorganic sulfide. It catalyzes the formation of cysteine from O-acetylserine and inorganic sulfide. Gene expression is predominant in the root cortex and the xylem parenchyma. Gene expression is induced in leave, stems and roots by high salt and heavy metal stresses, mediated by ABA. Lines carrying semi-dominant mutations exhibit early senescence.
    Computational Description
    O-acetylserine (thiol) lyase (OAS-TL) isoform A1 (OASA1); CONTAINS InterPro DOMAIN/s: Cysteine synthase/cystathionine beta-synthase P-phosphate-binding site (InterPro:IPR001216), Cysteine synthase A (InterPro:IPR005859), Pyridoxal phosphate-dependent enzyme, beta subunit (InterPro:IPR001926), Cysteine synthase K/M (InterPro:IPR005856); BEST Arabidopsis thaliana protein match is: O-acetylserine (thiol) lyase (OAS-TL) isoform A2 (TAIR:AT3G22460.1).
    Link
    InterPro Scan - TAIR