K35706

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K35706

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage
Phenotypes
T1  
rosette leaf (PO:0000014)
  • decreased size (PATO:0000587)
    (Rosette leaf::Shape::small)
  • increased width (PATO:0000600)
    (Rosette leaf::Shape::wide)
  • dark green (PATO:0001249)
    (Rosette leaf::Color::dark)
  • abnormal (PATO:0000460)
    (Rosette leaf::Others)
sporophyte vegetative stage (PO:0007134)
  • decreased speed (PATO:0000304)
    (Adult plant::Growth::slow)
seed (PO:0009010)
  • present in fewer numbers in organism (PATO:0001997)
    (Seed::Number::few)
petal (PO:0009032)
  • abnormal (PATO:0000460)
    (Flower::Shape::petal)
stem (PO:0009047)
  • abnormal (PATO:0000460)
    (Stem::Others)
T2  
cauline leaf (PO:0000013)
  • dark green (PATO:0001249)
    (Cauline leaf::Color::dark)
rosette leaf (PO:0000014)
  • dark green (PATO:0001249)
    (Rosette leaf::Color::dark)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K35706

Determination of introduced cDNA(s)

  • J033117K11 - Rice Full-length cDNAs - Original: J033117K11

    full reading [+] show sequence

    Nucleotide sequence: J033117K11 - full reading
    GACCTTCTCC TCCCTCGTCT CCTCTCCTCG TTCGGACCGA CGGCGACGGC 
    CGCCTAAATT ATCCATACCT CCCCTCCGCC GTGGCCACCA GCCACCTCCA
    CGAATCCAAT TCCAACATCT CCTCCTCCTC CTCCGCCGCC GCCGGGGGGA
    TCCGCGCTGC GCCGTGGTAA GCCCATCTCT CTCCCGGCCT TCCCGGCTCG
    ACGTTCGTTG TGGGGGGCGT GTTTTGCGCC CGAGGATTGG TGGGTTGGCG
    GGGGGATGGG GATTGGGGCG TCGGACGAGC TGCTGGGCAC GTTCGTGCCG
    ATCGCCGTGT ACTGGCTCTA CTCGGGGCTG TACCTCGCGC TGGACGGCGT
    GGAGCGGCTG GACGTGTACC GGCTGCACCC CAGGGAGGAG GAGGCCGCCA
    AGAACGTCGT CTCCAGGGGC ACCGTCGTCA GGGGCGTCCT CGTCCAGCAG
    GCCTTCCAGG TCGCCGTCTC GCTCACCCTC TTCGCGGTTA TTGGTGATGA
    AAGTGATATT GAACAGAAGC AACCTTCTGC CCTTGTGATA TTGCTGCAAT
    TTGCCATTGC AATGTTCGTT ATGGATACAT GGCAGTACTT CATGCACAGA
    TACATGCACA TCAACAAGTT CCTATATAAA CATATCCACT CCAAGCACCA
    CACTCTTGTA GTCCCTTATT CCTTTGGAGC TCTTTACAAC CATCCTCTTG
    AGGGTCTTAT TTTGGACACC ATTGGTGGTG CTCTCTCATT CCTCGTCTCT
    GGTATGACAC CACGGACATC CATATTCTTT TTCTCGTTCG CAACCATCAA
    GACAGTGGAT GATCATTGTG GTCTGTGGCT TCCTGGTAAC ATCCTCCATG
    CGCTGTTCAA CAACAACAGC GCTTATCATG ACATTCATCA TCAGCTCTAT
    GGAAACAAGT ACAACTTTTC ACAACCATTT TTTGTCATTT GGGACAAGAT
    ACTTGGAACT TACATGCCTT ACTCTATCGA GCACAGAAAA GGAGGAGGGT
    TTGAGTCACG GCCAGTTAAA CTCAACATAG CAGAGCAGAC CAAGACTGAT
    TAGTGTATTC TCGGTCCACT ACTCTCGGCT CTGTGGTACC CTGATCTTCT
    AACTACACTG TTGGTGTGAA ATTATCACCT TGTTCGTATA GTGATGTTTT
    TCTTGTTCTC TATATTCTTC TTCTGTTACC TTTTGGGAGG GGTTCTCCTT
    GTACCAAGGT ATAGTGAGTA AATTAGTGTA CTATATCAAA GAATACTAAA
    GCACTAAGAT CCACTTGGAT CTGTTTCCTG GAGATTGGCT GCATGTATTG
    AGCTTACATG CATGCCAATG TTAACAAGAG AAGATGATAT TTACACTGTA
    TGCAGCTATG CAGTAAGCCA GTAACTTTTG TGGTGC

    Gene models with high sequence identity

    1. AT1G69640.1 [+] show detail - sphingoid base hydroxylase 1
      E-value: 3e-108; Score: 389.00

     

    InterPro Scan Digest

    Program Description E-value
    HMMPanther SUR2 HYDROXYLASE/DESATURASE 8.1e-132
    HMMPanther STEROL DESATURASE 8.1e-132
    HMMPfam Fatty acid hydroxylase 3.6e-18

    See the detailed result >

    AT1G69640.1

    Model type
    Protein coding
    Short Description
    sphingoid base hydroxylase 1
    Curator Summary
    Encodes one of the two redundant sphingoid base hydroxylases (SBH). Involved in sphingolipid trihydroxy long-chain base (4-hydroxysphinganine) biosynthesis. Double mutants of SBHs were dwarfed and not able to progress from vegetative to reproductive growth.
    Computational Description
    sphingoid base hydroxylase 1 (SBH1); CONTAINS InterPro DOMAIN/s: Fatty acid hydroxylase (InterPro:IPR006694); BEST Arabidopsis thaliana protein match is: sphingoid base hydroxylase 2 (TAIR:AT1G14290.1); Has 2400 Blast hits to 2339 proteins in 395 species: Archae - 0; Bacteria - 406; Metazoa - 416; Fungi - 708; Plants - 429; Viruses - 3; Other Eukaryotes - 438 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR