K35706

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Phenotypes

Morphological phenotypes

Generation	Anatomical entity /structure development stage	Phenotypes
T1
	rosette leaf (PO:0000014)	decreased size (PATO:0000587) (Rosette leaf::Shape::small) increased width (PATO:0000600) (Rosette leaf::Shape::wide) dark green (PATO:0001249) (Rosette leaf::Color::dark) abnormal (PATO:0000460) (Rosette leaf::Others)
	sporophyte vegetative stage (PO:0007134)	decreased speed (PATO:0000304) (Adult plant::Growth::slow)
	seed (PO:0009010)	present in fewer numbers in organism (PATO:0001997) (Seed::Number::few)
	petal (PO:0009032)	abnormal (PATO:0000460) (Flower::Shape::petal)
	stem (PO:0009047)	abnormal (PATO:0000460) (Stem::Others)
T2
	cauline leaf (PO:0000013)	dark green (PATO:0001249) (Cauline leaf::Color::dark)
	rosette leaf (PO:0000014)	dark green (PATO:0001249) (Rosette leaf::Color::dark)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K35706

Determination of introduced cDNA(s)

J033117K11 - Rice Full-length cDNAs - Original: J033117K11

full reading [+] show sequence

GACCTTCTCC TCCCTCGTCT CCTCTCCTCG TTCGGACCGA CGGCGACGGC 
CGCCTAAATT ATCCATACCT CCCCTCCGCC GTGGCCACCA GCCACCTCCA 
CGAATCCAAT TCCAACATCT CCTCCTCCTC CTCCGCCGCC GCCGGGGGGA 
TCCGCGCTGC GCCGTGGTAA GCCCATCTCT CTCCCGGCCT TCCCGGCTCG 
ACGTTCGTTG TGGGGGGCGT GTTTTGCGCC CGAGGATTGG TGGGTTGGCG 
GGGGGATGGG GATTGGGGCG TCGGACGAGC TGCTGGGCAC GTTCGTGCCG 
ATCGCCGTGT ACTGGCTCTA CTCGGGGCTG TACCTCGCGC TGGACGGCGT 
GGAGCGGCTG GACGTGTACC GGCTGCACCC CAGGGAGGAG GAGGCCGCCA 
AGAACGTCGT CTCCAGGGGC ACCGTCGTCA GGGGCGTCCT CGTCCAGCAG 
GCCTTCCAGG TCGCCGTCTC GCTCACCCTC TTCGCGGTTA TTGGTGATGA 
AAGTGATATT GAACAGAAGC AACCTTCTGC CCTTGTGATA TTGCTGCAAT 
TTGCCATTGC AATGTTCGTT ATGGATACAT GGCAGTACTT CATGCACAGA 
TACATGCACA TCAACAAGTT CCTATATAAA CATATCCACT CCAAGCACCA 
CACTCTTGTA GTCCCTTATT CCTTTGGAGC TCTTTACAAC CATCCTCTTG 
AGGGTCTTAT TTTGGACACC ATTGGTGGTG CTCTCTCATT CCTCGTCTCT 
GGTATGACAC CACGGACATC CATATTCTTT TTCTCGTTCG CAACCATCAA 
GACAGTGGAT GATCATTGTG GTCTGTGGCT TCCTGGTAAC ATCCTCCATG 
CGCTGTTCAA CAACAACAGC GCTTATCATG ACATTCATCA TCAGCTCTAT 
GGAAACAAGT ACAACTTTTC ACAACCATTT TTTGTCATTT GGGACAAGAT 
ACTTGGAACT TACATGCCTT ACTCTATCGA GCACAGAAAA GGAGGAGGGT 
TTGAGTCACG GCCAGTTAAA CTCAACATAG CAGAGCAGAC CAAGACTGAT 
TAGTGTATTC TCGGTCCACT ACTCTCGGCT CTGTGGTACC CTGATCTTCT 
AACTACACTG TTGGTGTGAA ATTATCACCT TGTTCGTATA GTGATGTTTT 
TCTTGTTCTC TATATTCTTC TTCTGTTACC TTTTGGGAGG GGTTCTCCTT 
GTACCAAGGT ATAGTGAGTA AATTAGTGTA CTATATCAAA GAATACTAAA 
GCACTAAGAT CCACTTGGAT CTGTTTCCTG GAGATTGGCT GCATGTATTG 
AGCTTACATG CATGCCAATG TTAACAAGAG AAGATGATAT TTACACTGTA 
TGCAGCTATG CAGTAAGCCA GTAACTTTTG TGGTGC

Gene models with high sequence identity

AT1G69640.1 [+] show detail - sphingoid base hydroxylase 1
E-value: 3e-108; Score: 389.00

InterPro Scan Digest

Program	Description	E-value
HMMPanther	SUR2 HYDROXYLASE/DESATURASE	8.1e-132
HMMPanther	STEROL DESATURASE	8.1e-132
HMMPfam	Fatty acid hydroxylase	3.6e-18

See the detailed result >

AT1G69640.1

Model type: Protein coding
Short Description: sphingoid base hydroxylase 1
Curator Summary: Encodes one of the two redundant sphingoid base hydroxylases (SBH). Involved in sphingolipid trihydroxy long-chain base (4-hydroxysphinganine) biosynthesis. Double mutants of SBHs were dwarfed and not able to progress from vegetative to reproductive growth.
Computational Description: sphingoid base hydroxylase 1 (SBH1); CONTAINS InterPro DOMAIN/s: Fatty acid hydroxylase (InterPro:IPR006694); BEST Arabidopsis thaliana protein match is: sphingoid base hydroxylase 2 (TAIR:AT1G14290.1); Has 2400 Blast hits to 2339 proteins in 395 species: Archae - 0; Bacteria - 406; Metazoa - 416; Fungi - 708; Plants - 429; Viruses - 3; Other Eukaryotes - 438 (source: NCBI BLink).
Link: InterPro Scan - TAIR