K35721

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K35721

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T2 seed (PO:0009010)
  • low brightness (PATO:0000327)
    (Seed::Color::dark)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K35721

Determination of introduced cDNA(s)

  • J023002E06 - Rice Full-length cDNAs - Original: J023002E06

    full reading [+] show sequence

    Nucleotide sequence: J023002E06 - full reading 
    GTGTGCGTGT TGCTGTTCAG TGCAAGGTTG CCTCTGATCT GCCCAAGCTT 
    GTTGAGGGTT TGAAGCGTCT GGCGAAGTCT GATCCTATGG TGCTCTGTAC 
    TATTGAGGAG TCTGGTGAGC ATATCATTGC TGGTGCTGGA GAGCTTCACC 
    TTGAAATTTG CTTGAAGGAT CTGCAGGAAG ATTTCATGGG TGGTGCTGAA 
    ATCATTGTTT CCCCACCTGT TGTTTCTTTC CGTGAGACTG TCCTTGAGAA 
    GTCGTGCCGT ACTGTTATGA GCAAGTCCCC GAACAAGCAC AACCGTCTCT 
    ACATGGAAGC TCGCCCCTTG GAGGAGGGAT TGGCTGAGGC CATTGATGAC 
    GGTCGTATTG GCCCACGTGA TGATCCCAAG GTGCGCTCCA AGATCCTGTC 
    TGAGGAGTTC GGCTGGGACA AGGATCTTGC AAAGAAGATT TGGTGTTTTG 
    GACCTGAGAC AACTGGCCCC AACATGGTGG TTGATATGTG TAAGGGAGTT 
    CAGTATCTGA ATGAAATCAA GGATTCCGTT GTGGCTGGTT TCCAGTGGGC 
    ATCAAAGGAG GGTGCTCTGG CTGAGGAGAA CATGCGTGGT ATCTGCTTTG 
    AGGTGTGCGA TGTTGTTCTC CATGCTGATG CTATTCACAG AGGTGGTGGC 
    CAGGTCATTC CCACGGCCAG GAGGGTCATC TATGCTTCTC AGCTCACAGC 
    AAAGCCCAGG CTTCTTGAGC CCGTCTACCT GGTGGAGATC CAGGCCCCTG 
    AGAATGCCCT TGGTGGTATC TATGGTGTTC TGAACCAGAA GAGAGGACAT 
    GTGTTCGAGG AGATGCAGAG GCCTGGTACC CCTCTGTACA ACATCAAGGC 
    TTACCTCCCT GTCATCGAGT CCTTTGGGTT CTCAAGCCAG CTTAGGGCTG 
    CGACATCTGG CCAGGCCTTC CCCCAGTGTG TGTTCGACCA TTGGGACATG 
    ATGACCTCTG ATCCTCTTGA AGCTGGCTCG CAGGCTTCAA CCCTGGTGCA 
    GGATATCCGC AAGAGGAAGG GTCTCAAGGA GCAGATGACT CCCCTCTCCG 
    ATTTCGAGGA CAAGCTCTAA ATTTTTGGCC GCCGCATGTG TTGTGCCGTT 
    ACCCTTACAT GTTCTAGTTG GTCTACTGCT AAGAGAACTG CTTTCGTTGT 
    CGTATTTCAT TTCCCTTTTG TCGAATGCAT GTGTTTCACA TCAACAATCC 
    TATGTTCGCG TGCTACTGAG AAACTATTTT TACTCTGTCG TGATGTAACG 
    GATGTGGAAC CTGGATCATC TTTTTGCTGT CTAAGTTATA GTGTTCACAC 
    T

    Gene models with high sequence identity

    1. AT1G56070.1 [+] show detail - Ribosomal protein S5/Elongation factor G/III/V family protein
      E-value: 0; Score: 669.00

     

    InterPro Scan Digest

    Program Description E-value
    HMMPfam Translation elongation factor EFG/EF2, domain IV 8.3e-38
    HMMPfam Translation elongation factor EFG/EF2, C-terminal 2.6e-21
    HMMPanther EUKARYOTIC TRANSLATION ELONGATION FACTOR 2 2.6e-230
    HMMPanther TRANSLATION FACTOR 2.6e-230
    HMMSmart Translation elongation factor EFG/EF2, domain IV 8.1e-26
    HMMSmart Translation elongation factor EFG/EF2, C-terminal 5.1e-19
    superfamily Ribosomal protein S5 domain 2-type fold 1.8e-72
    superfamily Elongation factor G/III/V 1.5e-32
    superfamily Elongation factor G/III/V 1.7e-17
    Gene3D no description 2.4e-15
    Gene3D Translation elongation factor EFG/EF2, C-terminal 9.2e-36

    See the detailed result >

    AT1G56070.1

    Model type
    Protein coding
    Short Description
    Ribosomal protein S5/Elongation factor G/III/V family protein
    Curator Summary
    encodes a translation elongation factor 2-like protein that is involved in cold-induced translation. Mutations in this gene specifically blocks low temperature-induced transcription of cold-responsive genes but induces the expression of CBF genes and mutants carrying the recessive mutations fail to acclimate to cold and is freezing sensitive.
    Computational Description
    LOW EXPRESSION OF OSMOTICALLY RESPONSIVE GENES 1 (LOS1); FUNCTIONS IN: translation factor activity, nucleic acid binding, copper ion binding, translation elongation factor activity; INVOLVED IN: response to cold; LOCATED IN: cytosol, nucleolus, chloroplast, plasma membrane, membrane; EXPRESSED IN: 29 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Protein synthesis factor, GTP-binding (InterPro:IPR000795), Translation elongation factor EFG/EF2, domain IV (InterPro:IPR005517), Translation elongation factor EFG/EF2, C-terminal (InterPro:IPR000640), Translation elongation factor EFTu/EF1A, domain 2 (InterPro:IPR004161), Ribosomal protein S5 domain 2-type fold (InterPro:IPR020568), Ribosomal protein S5 domain 2-type fold, subgroup (InterPro:IPR014721), Elongation factor G/III/V (InterPro:IPR009022), Translation elongation/initiation factor/Ribosomal, beta-barrel (InterPro:IPR009000); BEST Arabidopsis thaliana protein match is: Ribosomal protein S5/Elongation factor G/III/V family protein (TAIR:AT3G12915.1); Has 64297 Blast hits to 57476 proteins in 4618 species: Archae - 1324; Bacteria - 41101; Metazoa - 3172; Fungi - 1934; Plants - 1269; Viruses - 1; Other Eukaryotes - 15496 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR