K35924

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K35924

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T2 seed (PO:0009010)
  • low brightness (PATO:0000327)
    (Seed::Color::dark)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K35924

Determination of introduced cDNA(s)

  • J023036J02 - Rice Full-length cDNAs - Original: J023036J02

    full reading [+] show sequence

    Nucleotide sequence: J023036J02 - full reading 
    GCTACTTTTA CGACACCTCC CCACCCCCCG TCTCCTTCTC CCTCGTCTCC 
    AGATCCGTGG GTCGAAGCAG CGGCGGCGCC CGATCCACGG CGGTCACTCC 
    CTCCGCCATG AATCCCGAGT ACGACTACCT TTTCAAACTT CTCCTCATTG 
    GTGATTCTGG TGTTGGGAAA TCGTGCTTGC TTCTCAGATT TGCGGATGAC 
    TCATACCTGG ACAGCTATAT CAGCACAATT GGAGTTGATT TTAAAATACG 
    GACAGTAGAG CAGGATGGGA AGACCATCAA GCTTCAAATC TGGGATACTG 
    CTGGACAAGA ACGTTTCAGG ACGATTACAA GCAGCTATTA CCGGGGAGCT 
    CATGGAATAA TTATTGTATA CGATGTGACA GACCAAGAAA GCTTCAACAA 
    TGTGAAGCAG TGGTTGAATG AAATTGATCG TTATGCAAGT GACAATGTAA 
    ACAAGCTCCT TGTTGGGAAC AAGAGCGACC TAACTGCCAA CAAAGTTGTG 
    TCATCTGAAA CAGCTAAGGC GTTTGCTGAT GAGATGGGCA TCCCCTTCAT 
    GGAGACAAGT GCCAAGAACG CCACTAATGT GGAGCAGGCC TTCATGGCTA 
    TGGCCGCATC CATCAAGGAC AGGATGGCTA GCCAACCGGC CGCTTCAAAT 
    GCAAGGCCAC CGACCGTGCA GATCCGCGGG CAACCTGTCA ACCAGAAGAC 
    GTCGTGCTGC TCGTCCTAAA GATAACCAAG TCTTTCCTTC CATGTAAAAT 
    TCGACGTATG TTACTACTGT TTGCTCACTA CAACGTATTT GTGATATTCA 
    TTTGAACGCC CTTGATACCA TCTTTTCTTT AATCAGATGG TTAGGCAGCT 
    GCAAATATAT ATGTAACCTT GCTTTGTTGC TCTTTGCTCA CGAATCGAGA 
    GGAGCAAGCA GAACCAGTGT ATTTGATTCT TTTCACGCTT ACATTTTTCC 
    CATTTCATTG CATACAAGTA TTCTGTGTTG AGGTTTAGTT AGACCGGACA 
    GCGGACTGAT TACAACAATT TTACAACTTG TCACAGGTGT TGG

    Gene models with high sequence identity

    1. AT1G02130.1 [+] show detail - RAS 5
      E-value: 1e-100; Score: 363.00

     

    InterPro Scan Digest

    Program Description E-value
    HMMTigr Small GTP-binding protein 2.2e-33
    Gene3D no description 2.9e-69
    superfamily P-loop containing nucleoside triphosphate hyd 1.3e-70
    HMMPanther RAS-RELATED PROTEIN RAB-1 4.7e-99
    HMMPanther RAS-RELATED GTPASE 4.7e-99
    ProfileScan Small GTPase 39.297
    FPrintScan Ras GTPase 4.9e-46
    FPrintScan Ras GTPase 4.9e-46
    FPrintScan Ras GTPase 4.9e-46
    FPrintScan Ras GTPase 4.9e-46
    FPrintScan Ras GTPase 4.9e-46
    HMMSmart ADP-ribosylation factor 0.0031
    HMMSmart Ras small GTPase, Ras type 2.2e-36
    HMMSmart Ras small GTPase, Rab type 1.3e-108
    HMMSmart Small GTPase, Rho type 5.4e-16
    HMMSmart Ran GTPase 9.4e-06
    HMMPfam Ras 4.6e-66

    See the detailed result >

    AT1G02130.1

    Model type
    Protein coding
    Short Description
    RAS 5
    Curator Summary
    Belongs to the Rab1 GTPase subfamily. This small GTP-binding protein is required in ER to Golgi transportation.
    Computational Description
    RAS 5 (RA-5); FUNCTIONS IN: GTP binding; INVOLVED IN: response to cadmium ion, ER to Golgi vesicle-mediated transport; LOCATED IN: plasma membrane, vacuole; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Ras GTPase (InterPro:IPR001806), Small GTP-binding protein (InterPro:IPR005225), Small GTPase (InterPro:IPR020851), Ras (InterPro:IPR013753), Ras small GTPase, Rab type (InterPro:IPR003579); BEST Arabidopsis thaliana protein match is: RAB GTPase homolog 1A (TAIR:AT5G47200.1); Has 30420 Blast hits to 30350 proteins in 816 species: Archae - 27; Bacteria - 199; Metazoa - 15882; Fungi - 4318; Plants - 3658; Viruses - 20; Other Eukaryotes - 6316 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR