K36608

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K36608

Phenotypes

Morphological phenotypes

Specific phenotypess were NOT observed.

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K36608

Determination of introduced cDNA(s)

  • J033128M07 - Rice Full-length cDNAs - Original: J033128M07

    full reading [+] show sequence

    Nucleotide sequence: J033128M07 - full reading 
    GGCGAGCTTA TCACTTATCA TCAATCGGCG CCTCCCTCTC TCTCGCTCAC 
    CGCCTCCTTC TCCTCGCGAT CTATCCAAAC AAGGGGCTCG CTAAGATCGG 
    CATGTCGGAC TCTGAGGAGC ACCATTTCGA GTCGAAGGCC GACGCTGGGG 
    CGTCCAAGAC CTATCCCCAG CAGGCCGGAA CCATCCGCAA GAATGGGTAT 
    ATTGTTATCA AGAACCGCCC CTGCAAGGTG GTGGAGGTTT CTACCTCGAA 
    GACTGGTAAG CACGGTCATG CCAAGTGTCA CTTTGTTGCC ATAGATATAT 
    TCAATGGTAA AAAGCTTGAG GATATTGTTC CTTCGTCCCA CAACTGTGAT 
    GTTCCACATG TGAACCGCAC AGAGTACCAG CTGATTGACA TATCAGAGGA 
    TGGATTCGTG AGCCTTCTTA CTGAGAGTGG TAACACTAAG GATGATCTTA 
    GACTCCCAAC TGATGACAGT CTCCTGGGTC AGATCGAGAC TGGATTTGGT 
    GAAGGCAAGG ATCTTGTTGT GACTGTCATG TCCGCCATGG GGGAGGAGCA 
    GATCTGTGCG CTGAAGGACA TTGGCCCCAA GTAACTCCCT CAAGTGGAAG 
    GCAGAACAGA GGGGCAACTC CCTCAAGTGG AAGGCAGAAC AGAGGGGTAA 
    CTCCCTCAAG TGGAAGGCAG AACAGAGGGA TCCTTATGTA TCAAACCTAA 
    ACTAAAAAAC AAAGACATGT ATTGAGAAGG AATGCTACAA AAGACATCCA 
    CCCAGCTTGA AGCCGCAGTA ACAAGTGCAG TGATTGCTGG GTGTGGCATT 
    ATCTGATTAC CGCTACCCCA TCATGACATT TGGGATCCTG TATTTTCCAT 
    GGTTTTATTT GTGCCCTCTG TTTGGTATCA ATAATTGATT TGGAACGGTG 
    GAAACCATAT ACTGAAGCTG AAGATTATCA TTGGGTCATT TCGTGCCTAT 
    GGATTTTTCT CTGAAAATTT CCCTAGTCAC TGTTTAGTCT GCAACC

    Gene models with high sequence identity

    1. AT1G13950.1 [+] show detail - eukaryotic elongation factor 5A-1
      E-value: 3e-76; Score: 282.00

     

    InterPro Scan Digest

    Program Description E-value
    PatternScan Translation elongation factor, IF5A, hypusine site 0
    HMMPfam Translation elongation factor, IF5A C-terminal 1.8e-29
    HMMPfam KOW 6.8e-05
    Gene3D Translation protein SH3-like, subgroup 2e-27
    Gene3D Nucleic acid-binding, OB-fold 1.4e-25
    superfamily Translation protein SH3-like 2.3e-28
    superfamily Nucleic acid-binding, OB-fold-like 2.2e-27
    HMMPanther INITIATION FACTOR 5A 8.3e-111
    HMMPanther Translation elongation factor, IF5A 8.3e-111
    HMMTigr Translation elongation factor, IF5A 1.3e-56
    HMMPIR Translation elongation factor, IF5A 5.6e-101

    See the detailed result >

    AT1G13950.1

    Model type
    Protein coding
    Short Description
    eukaryotic elongation factor 5A-1
    Curator Summary
    Encodes eukaryotic translation initiation factor 5A (EIF-5A).In mammalian cells it functions as a shuttle protein that translocates mRNA from the nucleus to cytoplasmic ribosomes. Overexpression results in an increase in both primary and secondary xylem formation. In RNAi suppressed lines, xylem formation is reduced.
    Computational Description
    eukaryotic elongation factor 5A-1 (ELF5A-1); FUNCTIONS IN: ribosome binding, RNA binding, translation elongation factor activity, translation initiation factor activity; INVOLVED IN: translational initiation, xylem development; LOCATED IN: cellular_component unknown; EXPRESSED IN: 18 plant structures; EXPRESSED DURING: 11 growth stages; CONTAINS InterPro DOMAIN/s: Nucleic acid-binding, OB-fold (InterPro:IPR012340), Translation elongation factor, IF5A, hypusine site (InterPro:IPR019769), Translation protein SH3-like, subgroup (InterPro:IPR014722), Translation elongation factor, IF5A (InterPro:IPR001884), Translation elongation factor, IF5A C-terminal (InterPro:IPR020189), Translation protein SH3-like (InterPro:IPR008991), Nucleic acid-binding, OB-fold-like (InterPro:IPR016027), KOW (InterPro:IPR005824); BEST Arabidopsis thaliana protein match is: eukaryotic elongation factor 5A-3 (TAIR:AT1G69410.1); Has 1356 Blast hits to 1355 proteins in 415 species: Archae - 264; Bacteria - 0; Metazoa - 366; Fungi - 246; Plants - 261; Viruses - 0; Other Eukaryotes - 219 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR