K36644

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Phenotypes

Morphological phenotypes

Generation	Anatomical entity /structure development stage	Phenotypes
T2	seed (PO:0009010)	low brightness (PATO:0000327) (Seed::Color::dark)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K36644

Determination of introduced cDNA(s)

J013124G04 - Rice Full-length cDNAs - Original: J013124G04

full reading [+] show sequence

GGTCTGGTCT GGGTCTGGTC GGTCGTCTTC CACTCCATCC GATCGACCAA 
CCAGCCGATG GGTTCTCGCG GCTGCTTCTG CAAGCGACCC GCGGCCATGG 
GCAATCTTCT TCTTCTTCTT CCCCACCCGG CCAAGGCCAA ATTGAGGCCT 
CCCACCACGG CGGCGAAGCG CGGTACACTC TTCTTCTGCA GCCTCAGCAC 
CGATGGTCCG TCGTCGACGA TGACCGTCTC CATCACTGGC GCCACCGGTT 
TCGTTGGCCG CAGATTGGTC CAAAAGCTTC TCTCCGAAGA TCACAAGGTT 
TGCGTCTTAA CGCGGTCCGC CAGCAAGGCC ACATCAGTTT TTCCGGCTTC 
CACATTTCCA GGAATTACAA TAGCCGAGCA GGGGGACTGG GATAAGTGCA 
TCCAAGGCTC CACTGCTGTT GTTAACTTGG CAGGCATGCC CATAAGCACA 
AGGTGGTCAC CTGAGATTAA GAAGGAAATC AAGGAAAGCC GAATCAACGT 
TACATCAAAA GTGGTGAATT ACATAAATAA TGCCAGTGGT GATGCTCGGC 
CCTCAGTTTT CGTGAGCGCA ACAGCCATTG GCTTCTATGG TACCAGTGAG 
ATCAGTAGCT TCGATGAGTC AAGTCCGTCA GGCAATGACT ATCTAGCAGA 
GGTGTGTCGA GAGTGGGAGG CCAGGGCATG CCAAGTAAAC CAAGAGGATG 
TTAGGCTTGT GCTTCTTCGG ATTGGGGTTG TTCTCGGCAA GGACGGCGGC 
GCTCTAGCCA AGATGATTCC CCTTTTTATG ATGTTTGCCG GCGGCCCCCT 
GGGGACGGGA CGGCAATGGT TTTCCTGGAT TCACCAGGAC GACCTGGTGG 
ACCTCATCTA CGAGTCCCTC AAGAACCCAT CATATAAAGG CGTGATAAAC 
GGAACCGCAC CCAACCCGGT TCGGCTGTCG GAGATGTGCG AGCGGCTGGG 
GCGAGTCGTC GGCCGCCCCT CCTGGCTGCC GGTGCCAGAA ATCGCCCTCA 
AAGCGGTGCT CGGAGAAGGT GCATCAGTGG TCCTGGAAGG GCAGAAGGTT 
CTTCCTGTGA AGGCCAAGCA ACTGGGCTTC TCCTACCGAT ACCCCTATGT 
ACAGGATGCA CTCCGGGCCA TTGCCAGGGA CCTATGACTG ATCGACTCAG 
ATGAATACTC AAATCGATCG ATCCGCCGTG CACGCACAAC AGTAGATATC 
AAAATTGAAA TATATTATAT ATACCAAAAG CAAACTATTA TTGTTGTTGG 
CTTGTATTAT CTCTGATTAA ACTAGGCCAT ACGTTTCATT GGATGGCGTA 
CGTCTTTCCA CCATTTTTAG ACTGAAGCAA ATATATGCAC GTGAATGAAT 
GAGC

Gene models with high sequence identity

AT2G21280.1 [+] show detail - NAD(P)-binding Rossmann-fold superfamily protein
E-value: 1e-119; Score: 427.00

InterPro Scan Digest

Program	Description	E-value
Gene3D	NAD(P)-binding domain	0.0000000000000012
HMMTigr	Sugar nucleotide epimerase YfcH, putative	4.9e-150
superfamily	NAD(P)-binding domain	3.3e-42
HMMPanther	Sugar nucleotide epimerase YfcH, putative	6.7e-78
HMMPfam	NAD-dependent epimerase/dehydratase	3.4e-18
HMMPfam	Domain of unknown function DUF1731, C-terminal	1.7e-16

See the detailed result >

AT2G21280.1

Model type: Protein coding
Short Description: NAD(P)-binding Rossmann-fold superfamily protein
Curator Summary: A nuclear-encoded, plastid-targeted protein (AtSulA) whose overexpression causes severe yet stochastic plastid (shown in chloroplasts and leucoplasts) division defects. The protein does not appear to interact with either AtFtsZ proteins when studied in a yeast two-hybrid system.
Computational Description: SULA; FUNCTIONS IN: coenzyme binding, binding, catalytic activity; INVOLVED IN: chloroplast fission; LOCATED IN: chloroplast, plastid, chloroplast envelope; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Sugar nucleotide epimerase YfcH, putative (InterPro:IPR010099), NAD-dependent epimerase/dehydratase (InterPro:IPR001509), Domain of unknown function DUF1731, C-terminal (InterPro:IPR013549), NAD(P)-binding domain (InterPro:IPR016040); BEST Arabidopsis thaliana protein match is: NAD(P)-binding Rossmann-fold superfamily protein (TAIR:AT4G33360.2); Has 4854 Blast hits to 4853 proteins in 1452 species: Archae - 38; Bacteria - 3329; Metazoa - 111; Fungi - 30; Plants - 185; Viruses - 0; Other Eukaryotes - 1161 (source: NCBI BLink).
Link: InterPro Scan - TAIR