K36845

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Phenotypes

Morphological phenotypes

Generation	Anatomical entity /structure development stage	Phenotypes
T2	seed (PO:0009010)	decreased size (PATO:0000587) (Seed::Shape::small) decreased length (PATO:0000574) (Seed::Shape::short)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K36845

Determination of introduced cDNA(s)

J023083E07 - Rice Full-length cDNAs - Original: J023083E07

full reading [+] show sequence

GAATTATTTT TTCCCATTTC GCTCACCTGT TCCCCTCCTC CGCCCTCTCC 
CCAACTCGCC ATTTTCCCCG TCTTCCACAG TGACAACCAC CACCCCACGC 
TTTCCCCTTC CTCCTCGCGA TCCAGCAGCG GGAGAGATGG ATCGGCCGCC 
GCCGGATCAG CAGCGGCAGA AGCAGGCGCC GCTCTTCTCG CCGTACCAGA 
TGCCCCGCTT CCGCCTCAAC CACCGGGTGG TGCTGGCGCC GATGACGCGG 
TGCAGGGCGA TCGGCGGGGT GCCCGGCCCG GCGCTGGCGG AGTACTACGC 
GCAGCGGACC ACCCAGGGTG GCCTGCTCAT CTCCGAGGGC ACCGTCGTCT 
CGCCCGCTGG CCCGGGGTTT CCTCATGTCC CTGGGATATA CAATCAAGAG 
CAGACTGATG CATGGAAGAA GGTGGTGGAT GCTGTTCATG CCAAGGGAGG 
CATCTTTTTC TGCCAGTTAT GGCATGTAGG CAGAGCTTCT CACCAAGTAT 
ACCAGCCAAA CGGTGCTGCA CCAATATCCT CAACTGATAA GCCAATATCA 
GCAAGATGGA GAATACTGAT GCCTGATGGC TCCTATGGCA AGTATCCTAA 
ACCTAGGCGC CTGGCAGCAT CGGAAATACC TGAAATTGTC GAACAATATC 
GTCAAGCCGC CATTAATGCC ATTGAAGCAG GTTTTGATGG CATTGAGATC 
CATGGTGCTC ATGGCTATAT CATTGATCAA TTCCTAAAGG ATGGAATCAA 
TGACCGCACT GACGAGTATG GTGGCTCACT TTCCAACCGC TGCCGGTTCC 
TACTTGAGGT AACTAGGGCT GTGGTTTCTG CCATTGGAGC AGACCGAGTC 
GCGGTGAGGA TATCACCAGC CATTGATCAC CTTGACGCCT ATGATTCAGA 
CCCCATTAAG CTCGGCATGG CCGTTGTTGA GCGGCTGAAT GCTCTCCAGC 
AGCAGTCAGG GCGGCTCGCC TACCTCCACG TCACGCAGCC ACGGTACACC 
GCCTACGGGC AGACCGAGTC TGGGCAGCAT GGCAGTGCCG AGGAGGAGAG 
CCGCCTGATG CGCACCCTCC GGGGCACGTA CCAGGGCACA TTCATGTGCA 
GTGGCGGCTA CACGCGGGAG CTTGGGTTGG AAGCAGTGGA GAGCGGCGAT 
GCCGACCTGG TGTCGTACGG GCGGCTCTTC ATATCAAACC CGGACCTGGT 
CGAGCGGTTC AGGCTGAACG CCGGGCTGAA CAAGTACGTG CGTAAGACAT 
TCTACACGCC CGATCCTGTC GTGGGTTACA CGGACTATCC GTTCCTCGGA 
CAGCCTAAGT CGCGGATGTA AATGTACTGC GGGATAAGTA AATCAAGGAT 
ACGATTGTGT ATTGTAAGGA AAATAAAGGG CAACGGTGAG GTGATTTAGC 
TTATTTTTGG GACCTAATTT GACGGTAATT TATTCTTTCA CCAAATCTGA 
TATGATCCAT TGCTGATTCA GTAGCTACTC TAGCCTATGT AAACCTTGGG 
TCATCAGCAC AGATTGTATC TATATGTGTA TTGCATAATG TTTCATATCA 
GTTGTCTAGC

Gene models with high sequence identity

AT2G06050.3 [+] show detail - oxophytodienoate-reductase 3
E-value: 8e-165; Score: 577.00

InterPro Scan Digest

Program	Description	E-value
HMMPfam	NADH:flavin oxidoreductase/NADH oxidase, N-terminal	1.9e-84
HMMPanther	12-OXOPHYTODIENOATE REDUCTASE OPR	6.3e-231
HMMPanther	NADH OXIDOREDUCTASE-RELATED	6.3e-231
Gene3D	Aldolase-type TIM barrel	1.5e-115
superfamily	FMN-linked oxidoreductases	7.1e-130

See the detailed result >

AT2G06050.3

Model type: Protein coding
Short Description: oxophytodienoate-reductase 3
Curator Summary: Encodes a 12-oxophytodienoate reductase that is required for jasmonate biosynthesis. Mutants are male sterile and defective in pollen dehiscence. Shows activity towards 2,4,6-trinitrotoluene.
Computational Description: oxophytodienoate-reductase 3 (OPR3); FUNCTIONS IN: 12-oxophytodienoate reductase activity; INVOLVED IN: response to jasmonic acid stimulus, response to fungus, jasmonic acid biosynthetic process, response to wounding, response to ozone; LOCATED IN: peroxisome; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 14 growth stages; CONTAINS InterPro DOMAIN/s: NADH:flavin oxidoreductase/NADH oxidase, N-terminal (InterPro:IPR001155), Aldolase-type TIM barrel (InterPro:IPR013785); BEST Arabidopsis thaliana protein match is: 12-oxophytodienoate reductase 2 (TAIR:AT1G76690.1); Has 13056 Blast hits to 13024 proteins in 2039 species: Archae - 127; Bacteria - 9671; Metazoa - 31; Fungi - 870; Plants - 454; Viruses - 0; Other Eukaryotes - 1903 (source: NCBI BLink).
Link: InterPro Scan - TAIR