K37826

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K37826

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T2 seed (PO:0009010)
  • decreased width (PATO:0000599)
    (Seed::Shape::narrow)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K37826

Determination of introduced cDNA(s)

  • J023037K22 - Rice Full-length cDNAs - Original: J023037K22

    full reading [+] show sequence

    Nucleotide sequence: J023037K22 - full reading 
    GTTGGACAAA ATGACTCCGG CGGCTGATGG CGACGACGAT GAGACGACAT 
    GCATCCGTGC CTTGGAGCTA ATCTTCACCT TTGTCGTTCC CATGACACTC 
    AAGGCGACGA TCAAGCTTGG CCTCCTCGAC GCACTCACCG GCGGCGGCCA 
    CGCACTCACC GCCGACGAGC TGGCCGCGGC CGCGCAACTG CCGGCCGAGG 
    CGGCGTCGTC GGTGGACCGC ATGCTGAGGC TCCTCGCGTC GTTGGATGTC 
    GTCAAGTGCG CCCCGACAGA CACTGGAGGC GAAGCAGCTG TCCGGCGGTA 
    CACGCCAGCG CCGGTGTGCA GGTGGTTTGC CGGCGAAAGG TCTCTGGCAC 
    CGCTGGCCAT GTTCCTCCTC GACGACGACT ACCTGAGCAC CTGGAATCAG 
    TTGCCGGCGG CGGTGGCCGG CGGCGACGGG CAGGTGGCGT TCGAGAAGGC 
    TCGCGGGATG CCCATGTTCG AGTACATGGG GACGAACCGC CGGCTGAACA 
    CGCTCTTCAA CCAGGCCATG GTGCAGCAGT CGACGGTGGT GATCGGCAAG 
    CTGCTCGAGC GATTCCAGGG GTTCGACGGC GTCAGCGTGC TCGTCGACGT 
    CGGCGGGGGC ACCGGCGCCA CCCTGGAGAT GATCACTTCG AGGTACAAGA 
    ACATCACCGG CGTTAACTTC GACCTGCCTC ATGTTATTGC TCAGGCTCCA 
    TCTCTTCCGG GTGTCAAACA TATAGCTGGA AATATGTTTG AGAGCGTACC 
    TAATGGAGAT GCAATTTTCT TGAAGTCGAT GCTCCACCTA CATAACGATG 
    AGGACTGCAT CAAGATCCTC AAGAAGTGCC ACCAAGCACT CACACACAAC 
    GGGAAGGTGA TTGCTGTTGA GATACTTCTC CCAGCGATTC CAGAGCCGGT 
    CCCAACAGCA CAAAACCCAT TCCGGATGGA CATGATCATG CTCAACAATC 
    ACTGGGGAGG AAAGGAGAGG ACGGAACCAG AGTTTGCTAA GCTAGCCGTG 
    GAATGTGGCT ACACCGGTGT ATTTCAAGCA ACCTATATTT TCGCTAACTA 
    TTGGGCTCTT GAGTTCAGCA AGTAGTTCTT CAAAATCATG TTAATTTGGT 
    TTTATATTTA TTATGTATTT CAATAAGTAT ATATGGCAAA GAGTAATTCC 
    GATGCCATGC AAATGGTTTG AAATTGTTTT TACTTGTGTA ATAAAAATAT 
    AAATTACCCC CTATAAATCG AACATCGTAC TGC

    Gene models with high sequence identity

    1. AT5G54160.1 [+] show detail - O-methyltransferase 1
      E-value: 2e-59; Score: 226.00

     

    InterPro Scan Digest

    Program Description E-value
    Gene3D Winged helix-turn-helix transcription repressor DNA-binding 4.5e-25
    Gene3D no description 1.7e-72
    HMMPfam O-methyltransferase, family 2 4.7e-75
    HMMPfam Plant methyltransferase dimerisation 1.1e-12
    superfamily S-adenosyl-L-methionine-dependent methyltrans 5.1e-72
    superfamily "Winged helix" DNA-binding domain 2e-27
    HMMPanther O-METHYLTRANSFERASE 2.1e-55
    HMMPIR O-methyltransferase, COMT, eukaryota 4.5e-116

    See the detailed result >

    AT5G54160.1

    Model type
    Protein coding
    Short Description
    O-methyltransferase 1
    Curator Summary
    A caffeic acid/5-hydroxyferulic acid O-methyltransferase. Interacts with 14-4-3 proteins in yeast 2 hybrid assay. AtOMT1 (At5g54160) encodes a flavonol 3
    Computational Description
    O-methyltransferase 1 (OMT1); FUNCTIONS IN: myricetin 3'-O-methyltransferase activity, quercetin 3-O-methyltransferase activity, caffeate O-methyltransferase activity; INVOLVED IN: lignin biosynthetic process, flavonol biosynthetic process; LOCATED IN: cytosol, nucleus, plasma membrane, cytoplasm; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 14 growth stages; CONTAINS InterPro DOMAIN/s: Winged helix-turn-helix transcription repressor DNA-binding (InterPro:IPR011991), Plant methyltransferase dimerisation (InterPro:IPR012967), O-methyltransferase, family 2 (InterPro:IPR001077), O-methyltransferase, COMT, eukaryota (InterPro:IPR016461); BEST Arabidopsis thaliana protein match is: O-methyltransferase family protein (TAIR:AT1G77520.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR