K42624

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K42624

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T1  
rosette leaf (PO:0000014)
  • decreased size (PATO:0000587)
    (Rosette leaf::Shape::small)
  • abnormal (PATO:0000460)
    (Rosette leaf::Others)
stem (PO:0009047)
  • decreased thickness (PATO:0000592)
    (Stem::Shape::thin)
  • abnormal (PATO:0000460)
    (Stem::Others)
T2 seed (PO:0009010)
  • increased size (PATO:0000586)
    (Seed::Shape::large)
  • increased length (PATO:0000573)
    (Seed::Shape::long)
  • decreased width (PATO:0000599)
    (Seed::Shape::narrow)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K42624

Determination of introduced cDNA(s)

  • J023125K01 - Rice Full-length cDNAs - Original: J023125K01

    full reading [+] show sequence

    Nucleotide sequence: J023125K01 - full reading 
    GATCCAAACA CCCATCCGCC GCCGCTGGCT GAACTCGCGG GTGATCTGAT 
    CGGAGCCGGA GGAGGAGGAG GAGGATGTTC GTGGAGGGCT TCAGGGTGGA 
    GAGCCCGCGC GTGCGGTACG GCGACGGGGA GATCGAGTCG GAGTACCGAT 
    ATGACACGAC GGAGGTGGTG GCGCCGCCGT CGCCGGAGAA GGGGTGGGTC 
    GTCCGCCCCA AGTCCGTCAC CTACCACTTC AAGACCACCA CCACCGTCCC 
    CAAGCTCGGG GTGATGCTGG TTGGATGGGG AGGCAACAAC GGCACCACGC 
    TCACGGCTGG TGTCATCGCC AACAGAGAGG GAATTTCATG GGCGACCAAG 
    GAGAAGGTGC ACAAGGCCAA CTACTTTGGT TCCCTGACAC AATCATCCAC 
    CATCAGGGTT GGGAGCTACA ACGGGGAGGA GATCTATGCG CCTTTTAAGA 
    GCCTTATACC CATGGTAAAC CCAAACGACA TCGTGTTCGG TGGGTGGGAC 
    ATCAGCAGCA TGAACCTGGC CGACGCCATG ACCAGGGCTC GGGTGCTGGA 
    CATCGACCTG CAGAAGCAGC TCCGGCATCA CATGGAGTCC ATGGTGCCCC 
    TCCCCGGCGT CTACAACCCT GACTTCATCG CCGCCAACCA GGGCTCCCGC 
    GCCAACAATG TCATCAAGGG CACCAAGAAG GAGCAGGTCG AACAGGTCAA 
    GAAGGACATC AGGGAGTTCA AGGAGAAGAG CAAGGTGGAC AAGGTGGTGG 
    TGCTGTGGAC GGCCAACACG GAGCGGTACA GCAACGTGGT GGCCGGGATG 
    AACGACACCA TGGACAACCT GCTGGCGTCG CTGGACAAGG ACGAGCCGGA 
    GATGTCGCCG TCGACGCTCT ACGCCATCGC CTGCGTCATG GAGGGCGTCC 
    CCTTCATCAA CGGCAGCCCC CAGAACACCT TCGTGCCCGG GCTGATCGAG 
    CTCGCCATCA AGAAGAACTC GGTGATCGGC GGCGACGACT TCAAGAGTGG 
    GCAGACCAAG ATGAAGTCCG TCCTCGTCGA CTTCCTCGTC GGCGCCGGCA 
    TCAAGCCGAC GTCGATCGCG AGCTACAACC ACCTAGGCAA CAACGACGGC 
    ATGAACCTGT CGGCGCCGCA GACGTTCCGG TCCAAGGAGA TCTCCAAGAG 
    CGGCGTCGTC GACGACATGG TCTCCAGCAA CGCCATCCTC TACGAGCCCG 
    GCGAGCACCC CGACCACGTC ATCGTCATCA AGTATATTCC ATACGTTGGG 
    GACAGCAAGA GGGCCATGGA CGAGTACACC TCGGAGATCT TCATGGGAGG 
    GAAGAACACC ATCGTGCTGC ACAACACCTG CGAGGACTCG CTCCTCGCCG 
    CGCCCATCAT CCTCGACCTC GTCCTCCTCG CCGAGCTCAG CACCAGGATC 
    CAGCTCAAAG CTGAAGACCA GGATAAGTAC CATTCTTTCC ATCCAGTGGC 
    TACCATCCTG AGCTACCTCA GCAAGGCTCC TCTTGTTCCC CCAGGCACAC 
    CAGTGGTGAA TGCACTGGCC AAGCAGAGGG CCATGCTGGA GAACATCCTG 
    AGGGCCTGTG TTGGACTGGC CCCTGAGAAC AACATGATGC TTGAGTACAA 
    GTGATATAGG CCTTGACAGG TCCTCTTATC AGGAGGGATG ATGAACAGCA 
    AGTGGTGGAG GAAGGTGACA GTTTTCTTCA ACAAGAAAAA GAACAAAAAA 
    AGAGAGAAAA TGAGGAACAC CTGAAAGAAA TAACAAGAGG CCAAGTGGGG 
    AAGCAAATGA AATGATCAAG TGATATTCAT TCAGAAAATG GGTGTCATTC 
    ACTTGGATCC TCTTAATTGG GGGCATCTAT TGACAGTTTG ACACTCTGCT 
    CCATGATTTT TTTTTTCATT TTTTGTTTTT TTAATTGAGA TGGAGACTTT 
    TTTCAGCTCT GCTGCTAGTA TGCTGTGAGC TGCATGAACA TTGGTTAATA 
    TTGTGTATGA TGATAGGTGA AGCAAGGAAC T

    Gene models with high sequence identity

    1. AT2G22240.1 [+] show detail - myo-inositol-1-phosphate synthase 2
      E-value: 0; Score: 854.00

     

    InterPro Scan Digest

    Program Description E-value
    HMMPanther Myo-inositol-1-phosphate synthase 0
    superfamily NAD(P)-binding domain 3e-168
    superfamily Glyceraldehyde-3-phosphate dehydrogenase-like 8.9e-46
    HMMPIR Myo-inositol-1-phosphate synthase 1.8e-275
    Coil coiled-coil 0
    Coil coiled-coil 0
    HMMPfam Myo-inositol-1-phosphate synthase 2.8e-119
    HMMPfam Myo-inositol-1-phosphate synthase, GAPDH-like 1.2e-46
    Gene3D NAD(P)-binding domain 1.1e-88

    See the detailed result >

    AT2G22240.1

    Model type
    Protein coding
    Short Description
    myo-inositol-1-phosphate synthase 2
    Curator Summary
    ** Referred to as MIPS1 in Mitsuhashi et al 2008. Myo-inositol-1-phosphate synthase isoform 2. Expressed in leaf, root and silique. Immunolocalization experiments with an antibody recognizing MIPS1, MIPS2, and MIPS3 showed endosperm localization.
    Computational Description
    myo-inositol-1-phosphate synthase 2 (MIPS2); CONTAINS InterPro DOMAIN/s: Myo-inositol-1-phosphate synthase (InterPro:IPR002587), Myo-inositol-1-phosphate synthase, GAPDH-like (InterPro:IPR013021), NAD(P)-binding domain (InterPro:IPR016040); BEST Arabidopsis thaliana protein match is: myo-inositol-1-phosphate synthase 1 (TAIR:AT4G39800.1); Has 1859 Blast hits to 1855 proteins in 512 species: Archae - 101; Bacteria - 453; Metazoa - 126; Fungi - 158; Plants - 764; Viruses - 0; Other Eukaryotes - 257 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR