K42624

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K42624

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage
Phenotypes
T1  
rosette leaf (PO:0000014)
  • decreased size (PATO:0000587)
    (Rosette leaf::Shape::small)
  • abnormal (PATO:0000460)
    (Rosette leaf::Others)
stem (PO:0009047)
  • decreased thickness (PATO:0000592)
    (Stem::Shape::thin)
  • abnormal (PATO:0000460)
    (Stem::Others)
T2 seed (PO:0009010)
  • increased size (PATO:0000586)
    (Seed::Shape::large)
  • increased length (PATO:0000573)
    (Seed::Shape::long)
  • decreased width (PATO:0000599)
    (Seed::Shape::narrow)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K42624

Determination of introduced cDNA(s)

  • J023125K01 - Rice Full-length cDNAs - Original: J023125K01

    full reading [+] show sequence

    Nucleotide sequence: J023125K01 - full reading
    GATCCAAACA CCCATCCGCC GCCGCTGGCT GAACTCGCGG GTGATCTGAT 
    CGGAGCCGGA GGAGGAGGAG GAGGATGTTC GTGGAGGGCT TCAGGGTGGA
    GAGCCCGCGC GTGCGGTACG GCGACGGGGA GATCGAGTCG GAGTACCGAT
    ATGACACGAC GGAGGTGGTG GCGCCGCCGT CGCCGGAGAA GGGGTGGGTC
    GTCCGCCCCA AGTCCGTCAC CTACCACTTC AAGACCACCA CCACCGTCCC
    CAAGCTCGGG GTGATGCTGG TTGGATGGGG AGGCAACAAC GGCACCACGC
    TCACGGCTGG TGTCATCGCC AACAGAGAGG GAATTTCATG GGCGACCAAG
    GAGAAGGTGC ACAAGGCCAA CTACTTTGGT TCCCTGACAC AATCATCCAC
    CATCAGGGTT GGGAGCTACA ACGGGGAGGA GATCTATGCG CCTTTTAAGA
    GCCTTATACC CATGGTAAAC CCAAACGACA TCGTGTTCGG TGGGTGGGAC
    ATCAGCAGCA TGAACCTGGC CGACGCCATG ACCAGGGCTC GGGTGCTGGA
    CATCGACCTG CAGAAGCAGC TCCGGCATCA CATGGAGTCC ATGGTGCCCC
    TCCCCGGCGT CTACAACCCT GACTTCATCG CCGCCAACCA GGGCTCCCGC
    GCCAACAATG TCATCAAGGG CACCAAGAAG GAGCAGGTCG AACAGGTCAA
    GAAGGACATC AGGGAGTTCA AGGAGAAGAG CAAGGTGGAC AAGGTGGTGG
    TGCTGTGGAC GGCCAACACG GAGCGGTACA GCAACGTGGT GGCCGGGATG
    AACGACACCA TGGACAACCT GCTGGCGTCG CTGGACAAGG ACGAGCCGGA
    GATGTCGCCG TCGACGCTCT ACGCCATCGC CTGCGTCATG GAGGGCGTCC
    CCTTCATCAA CGGCAGCCCC CAGAACACCT TCGTGCCCGG GCTGATCGAG
    CTCGCCATCA AGAAGAACTC GGTGATCGGC GGCGACGACT TCAAGAGTGG
    GCAGACCAAG ATGAAGTCCG TCCTCGTCGA CTTCCTCGTC GGCGCCGGCA
    TCAAGCCGAC GTCGATCGCG AGCTACAACC ACCTAGGCAA CAACGACGGC
    ATGAACCTGT CGGCGCCGCA GACGTTCCGG TCCAAGGAGA TCTCCAAGAG
    CGGCGTCGTC GACGACATGG TCTCCAGCAA CGCCATCCTC TACGAGCCCG
    GCGAGCACCC CGACCACGTC ATCGTCATCA AGTATATTCC ATACGTTGGG
    GACAGCAAGA GGGCCATGGA CGAGTACACC TCGGAGATCT TCATGGGAGG
    GAAGAACACC ATCGTGCTGC ACAACACCTG CGAGGACTCG CTCCTCGCCG
    CGCCCATCAT CCTCGACCTC GTCCTCCTCG CCGAGCTCAG CACCAGGATC
    CAGCTCAAAG CTGAAGACCA GGATAAGTAC CATTCTTTCC ATCCAGTGGC
    TACCATCCTG AGCTACCTCA GCAAGGCTCC TCTTGTTCCC CCAGGCACAC
    CAGTGGTGAA TGCACTGGCC AAGCAGAGGG CCATGCTGGA GAACATCCTG
    AGGGCCTGTG TTGGACTGGC CCCTGAGAAC AACATGATGC TTGAGTACAA
    GTGATATAGG CCTTGACAGG TCCTCTTATC AGGAGGGATG ATGAACAGCA
    AGTGGTGGAG GAAGGTGACA GTTTTCTTCA ACAAGAAAAA GAACAAAAAA
    AGAGAGAAAA TGAGGAACAC CTGAAAGAAA TAACAAGAGG CCAAGTGGGG
    AAGCAAATGA AATGATCAAG TGATATTCAT TCAGAAAATG GGTGTCATTC
    ACTTGGATCC TCTTAATTGG GGGCATCTAT TGACAGTTTG ACACTCTGCT
    CCATGATTTT TTTTTTCATT TTTTGTTTTT TTAATTGAGA TGGAGACTTT
    TTTCAGCTCT GCTGCTAGTA TGCTGTGAGC TGCATGAACA TTGGTTAATA
    TTGTGTATGA TGATAGGTGA AGCAAGGAAC T

    Gene models with high sequence identity

    1. AT2G22240.1 [+] show detail - myo-inositol-1-phosphate synthase 2
      E-value: 0; Score: 854.00

     

    InterPro Scan Digest

    Program Description E-value
    HMMPanther Myo-inositol-1-phosphate synthase 0
    superfamily NAD(P)-binding domain 3e-168
    superfamily Glyceraldehyde-3-phosphate dehydrogenase-like 8.9e-46
    HMMPIR Myo-inositol-1-phosphate synthase 1.8e-275
    Coil coiled-coil 0
    Coil coiled-coil 0
    HMMPfam Myo-inositol-1-phosphate synthase 2.8e-119
    HMMPfam Myo-inositol-1-phosphate synthase, GAPDH-like 1.2e-46
    Gene3D NAD(P)-binding domain 1.1e-88

    See the detailed result >

    AT2G22240.1

    Model type
    Protein coding
    Short Description
    myo-inositol-1-phosphate synthase 2
    Curator Summary
    ** Referred to as MIPS1 in Mitsuhashi et al 2008. Myo-inositol-1-phosphate synthase isoform 2. Expressed in leaf, root and silique. Immunolocalization experiments with an antibody recognizing MIPS1, MIPS2, and MIPS3 showed endosperm localization.
    Computational Description
    myo-inositol-1-phosphate synthase 2 (MIPS2); CONTAINS InterPro DOMAIN/s: Myo-inositol-1-phosphate synthase (InterPro:IPR002587), Myo-inositol-1-phosphate synthase, GAPDH-like (InterPro:IPR013021), NAD(P)-binding domain (InterPro:IPR016040); BEST Arabidopsis thaliana protein match is: myo-inositol-1-phosphate synthase 1 (TAIR:AT4G39800.1); Has 1859 Blast hits to 1855 proteins in 512 species: Archae - 101; Bacteria - 453; Metazoa - 126; Fungi - 158; Plants - 764; Viruses - 0; Other Eukaryotes - 257 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR