K45251

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K45251

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T1  
rosette leaf (PO:0000014)
  • decreased size (PATO:0000587)
    (Rosette leaf::Shape::small)
  • increased width (PATO:0000600)
    (Rosette leaf::Shape::wide)
stem (PO:0009047)
  • decreased thickness (PATO:0000592)
    (Stem::Shape::thin)
  • abnormal (PATO:0000460)
    (Stem::Shape::zigzag)
  • abnormal (PATO:0000460)
    (Stem::Others)
T2 seed (PO:0009010)
  • increased size (PATO:0000586)
    (Seed::Shape::large)
  • increased length (PATO:0000573)
    (Seed::Shape::long)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K45251

Determination of introduced cDNA(s)

  • J033036C04 - Rice Full-length cDNAs - Original: J033036C04

    full reading [+] show sequence

    Nucleotide sequence: J033036C04 - full reading 
    GAGTTTCCAC CTTTTCCCTC TCTTCCTCCG CTTATTAAAA GGCGGCGTCC 
    CTCCCCGTGG TGCTCGCCGC CGTATTCAAG CGGAGGAAGA GAGAGAGAGA 
    GAGAGAATCA AGGCAGTAGT CGTCTTCCTC TCCTCTCGCC GCCGCATCCG 
    ACCGGAGAAA GATCCCTTCT TTCCCTCCTC CAAGCCCGCC TCGGTATCCA 
    GAGATGGAGA CCTCGGCGGC GGCGGCGCCC AATGGCGGCG CGGCGGCGGC 
    GGAGCAGCAG CAGCGGCGTC GGCTGCCGGA CTTCCAGCAG TCGGTGAGGC 
    TCAAGTACGT GAAGCTGGGG TACCACTACC TCATCTCCCA CGGCATGTAC 
    CTGCTGCTGT CGCCGCTGAT GGCGCTGGTG GCCGTGCAGC TCTCCACCGT 
    GTCCCCGGGC GACATCGCCG ACCTGTGGGA GCAGCTCCGC TTCAACCTCC 
    TCTCCGTCGT CGCCTGCTCC ACCCTCCTCG TCTTCCTCTC CACCCTCTAC 
    TTCCTCACCC GCCCCCGCCC CGTCTACCTC CTCGACTTCG CCTGCTACAA 
    GCCCGACCCC CAGCGCAAGT GCACCCGCGA GACCTTCATG CGCTGCTCCA 
    GCCTCACCGG CTCCTTCACC GACGCCAACC TCGACTTCCA GCGCAAGATC 
    CTCGAGCGCT CCGGCCTCGG CGAGGACACC TACCTCCCTC CCGCCGTGCT 
    GCGCGTCCCG CCCAACCCGT GCATGGACGA GGCCCGCAAG GAGGCCCGCA 
    CCGTCATGTT CGGCGCCATT GACCAGCTTC TTGAGAAGAC GGGTGTGAAG 
    CCCAAGGACA TTGGTGTGGT TGTCGTCAAT TGCAGCTTGT TCAACCCGAC 
    GCCGTCGCTG TCCGCCATGG TTGTGAACCA CTACAAGCTG AGGGGGAATG 
    TCATCAGCTA CAACCTCGGC GGGATGGGGT GCAGTGCCGG CTTGCTGTCC 
    GTCGATCTCG CCAAGGACTT GCTGCAGGTG CACCCAAACT CGTACGCGCT 
    GGTGGTCAGC ATGGAGAACA TCACGCTCAA TTGGTACTTT GGCAACAACC 
    GGTCGATGCT CGTGTCGAAT TGCCTGTTCC GGATGGGCGG CGCCGCCATC 
    CTGCTGTCGA ACAGGCGGTC CGACCGCCGG AGGTCCAAGT ATGAGCTTGT 
    GCACACCGTG CGCACGCACA AGGGCGCCAA CGACAAGTGC TTTGGCTGTG 
    TCACCCAGGA GGAAGACGAG ATCGGCAAGA TCGGCGTGTC GCTCTCCAAG 
    GACCTGATGG CGGTGGCCGG AGACGCGCTG AAGACGAACA TCACCACCCT 
    CGGGCCGCTC GTGCTCCCGT TGTCGGAGCA GCTGCTCTTC ATGGCCACAT 
    TGGTTGCCAA GAAGGTCCTC AAGATGAAGA TCAAGCCATA CATTCCCGAT 
    TTCAAGCTGG CATTTGAGCA TTTCTGCATC CATGCCGGTG GCCGTGCCGT 
    GCTGGATGAG CTGGAGAAGA ACTTGGAGTT GACTGACTGG CACATGGAGC 
    CATCTAGGAT GACACTGTAC CGGTTTGGCA AAACATCGAG CAGCTCACTC 
    TGGTATGAGC TGGCGTACAC CGAGGCGAAG GGGAGGATCA GGAAGCGGGA 
    CAGGATCTGG CAGATTGCAT TTGGATCTGG TTTCAAGTGC AACAGCGCAG 
    TCTGGAAGGC GCTGCAAACA GTGAATCCAG CGAAGGAGAA GAACCCCTGG 
    ATGGATGAGA TTGACAACTT CCCGGTTGAA GTTCCAAAGA TATCAAAGGT 
    TGGAAATGCA TGACATGAAA TGCCTTGTTT GTCTCAAGAG TAGTAAAAAG 
    TATGGTGGGT TGAGTGGCAT TGTGCATTGG CATTGGTAGG GCAGGAAGTT 
    CATCTTCAGG GTTCTAAATC CATTTGCAAT TGTTCAAGAG TTCTTTGGTT 
    TTCAGTTGTA GCATCTGTGA GGGGAACTTT AAAAAGAAAA CTGTAGCTGA 
    TTCAATTAGG TAGAAAAAGG GTGTGTTTTC TTCACTTAGG GCTGTCTGAT 
    AATGCTGTAT TTATTCAGAT TGGAGAAAAC AATTGGAGTC TTCATGTGAC 
    GTTTATTCAT CGTTACCTGA TTGTGATTGC CCTTGTAGCA CTGTCACATT 
    CTCATTAAAT TTATCTCATT AAATTGATTG GTTT

    Gene models with high sequence identity

    1. AT2G26640.1 [+] show detail - 3-ketoacyl-CoA synthase 11
      E-value: 0; Score: 810.00

     

    InterPro Scan Digest

    Program Description E-value
    HMMPfam FAE1/Type III polyketide synthase-like protein 1.4e-146
    HMMPfam 3-Oxoacyl-[acyl-carrier-protein (ACP)] synthase III C-terminal 2.6e-08
    HMMPIR Very-long-chain 3-ketoacyl-CoA synthase 0
    superfamily Thiolase-like 2.9e-97
    Gene3D Thiolase-like, subgroup 8.8e-58
    Gene3D Thiolase-like, subgroup 5.2e-23

    See the detailed result >

    AT2G26640.1

    Model type
    Protein coding
    Short Description
    3-ketoacyl-CoA synthase 11
    Curator Summary
    Encodes KCS11, a member of the 3-ketoacyl-CoA synthase family involved in the biosynthesis of VLCFA (very long chain fatty acids).
    Computational Description
    3-ketoacyl-CoA synthase 11 (KCS11); FUNCTIONS IN: transferase activity, transferring acyl groups other than amino-acyl groups, catalytic activity; INVOLVED IN: response to cold, response to light stimulus, response to osmotic stress; LOCATED IN: membrane; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Thiolase-like (InterPro:IPR016039), Very-long-chain 3-ketoacyl-CoA synthase (InterPro:IPR012392), 3-Oxoacyl-[acyl-carrier-protein (ACP)] synthase III C-terminal (InterPro:IPR013747), FAE1/Type III polyketide synthase-like protein (InterPro:IPR013601), Thiolase-like, subgroup (InterPro:IPR016038); BEST Arabidopsis thaliana protein match is: 3-ketoacyl-CoA synthase 2 (TAIR:AT1G04220.1); Has 3664 Blast hits to 3650 proteins in 908 species: Archae - 0; Bacteria - 1588; Metazoa - 0; Fungi - 2; Plants - 1914; Viruses - 0; Other Eukaryotes - 160 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR