K45626

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K45626

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T1  
whole plant (PO:0000003)
  • decreased height (PATO:0000569)
    (Adult plant::Plant height::short)
cauline leaf (PO:0000013)
  • decreased size (PATO:0000587)
    (Cauline leaf::Shape::small)
  • present in fewer numbers in organism (PATO:0001997)
    (Cauline leaf::Number::few)
rosette leaf (PO:0000014)
  • decreased size (PATO:0000587)
    (Rosette leaf::Shape::small)
  • increased width (PATO:0000600)
    (Rosette leaf::Shape::wide)
  • upturned (PATO:0002031)
    (Rosette leaf::Shape::hyponastic)
  • desaturated green (PATO:0001272)
    (Rosette leaf::Color::pale)
stem (PO:0009047)
  • decreased thickness (PATO:0000592)
    (Stem::Shape::thin)
  • abnormal (PATO:0000460)
    (Stem::Others)
T2 seed (PO:0009010)
  • increased size (PATO:0000586)
    (Seed::Shape::large)
  • increased length (PATO:0000573)
    (Seed::Shape::long)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K45626

Determination of introduced cDNA(s)

  • J013044D12 - Rice Full-length cDNAs - Original: J013044D12

    full reading [+] show sequence

    Nucleotide sequence: J013044D12 - full reading 
    GTGTTGAGAA GAACATCCTT GTCTTTGACC TTGGTGGTGG GACATTCGAT 
    GTCAGTATCT TGACCATTGA CAACGGTGTG TTTGAGGTGT TGGCCACCAA 
    TGGTGACACT CATCTTGGAG GTGAGGACTT TGACCAGAGA ATCATGGAGT 
    ACTTCATCAA GTTGATCAAG AAGAAGTACA GCAAGGACAT CAGCAAGGAC 
    AACCGTGCTC TTGGAAAGCT GAGGAGGGAA GCTGAGCGTG CCAAGAGGGC 
    CCTCAGCAAC CAGCACCAGG TCCGTGTTGA GATTGAGTCC CTCTTTGACG 
    GGACCGATTT CTCGGAGCCG CTGACCCGTG CCAGATTTGA GGAGTTGAAC 
    AATGATCTGT TCCGCAAGAC CATGGGACCT GTCAAGAAGG CTATGGACGA 
    TGCTGGCCTT GAGAAGAGCC AGATTCATGA GATTGTTCTT GTCGGTGGTA 
    GCACCAGGAT TCCCAAGGTT CAGCAGCTCC TCAGAGACTA CTTCGAGGGC 
    AAGGAGCCCA ACAAGGGTGT CAACCCTGAT GAGGCTGTTG CCTATGGTGC 
    TGCGGTTCAG GGAAGCATTC TCAGCGGCGA GGGCGGTGAC GAAACCAAAG 
    ATATCCTTCT CCTGGATGTG GCACCCCTTA CCCTCGGTAT CGAGACTGTC 
    GGTGGAGTCA TGACCAAGTT GATCCCCAGG AACACCGTCA TCCCCACCAA 
    GAAGTCCCAG GTGTTCACCA CCTACCAGGA TCAGCAGACA ACCGTCTCCA 
    TCCAGGTCTT TGAGGGTGAG CGCAGCATGA CCAAGGACTG CCGTCTTCTC 
    GGCAAGTTCG ACCTATCTGG CATTCCAGCA GCACCAAGGG GCACCCCCCA 
    GATTGAGGTG ACCTTCGAGG TGGACGCCAA CGGCATCCTG AACGTGAAGG 
    CTGAGGACAA GGGCACCGGC AAGTCGGAGA AGATCACGAT CACGAACGAG 
    AAGGGGCGCC TGAGCCAGGA GGAGATCGAC CGGATGGTGC GCGAGGCGGA 
    GGAGTTTGCG GAGAAGGACA AGAAGGTGAA GGAGCGGATC GACGCCCGCA 
    ACCAGCTCGA GACCTACGTG TACAACATGA AGAACACCGT CGGCGACAAG 
    GACAAGCTGG CGGACAAGCT GGAGAGCGAG GAGAAGGAGA AGGTGGAGGA 
    GGCCCTCAAG GAGGCCCTCG AGTGGCTGGA CGAGAACCAG ACCGCCGAGA 
    AGGAGGAGTA CGAGGAGAAG CTCAAGGAGG TCGAGGCCGT CTGCAACCCC 
    ATCATCTCCG CCGTCTACCA GAGGACCGGT GGCGCCCCGG GTGGCGGCGC 
    CGACGGCGAG GGCGGCGTCG ACGACGAGCA TGACGAGCTG TAGATGATGA 
    TGATGATGAT CTGATTCGAT TGATTCAAAG GAGACAGCCA ATTTTTGTAA 
    GAGATGGCCG GTTACTTTTA GTGGGGTGAC TTACTACTAC CTTGGGTTGG 
    GGTTTAGGGG TAGCAAGAGG GGAGAGTTTT ATTTGCAGAA CACCAGAAAA 
    GAGACTAAAT CTCTCCTCTT GTTACTTGTG TTGCTCTCTT AGTTTGTTAC 
    TCTGTCGAGT GTAGAACAGC TCTTCCTTTT GCTACTTCCT TCTGATTTCC 
    ATGTGAACTG TGAAGAAGAA GAAGAAACAT AAGTAAATTT TAATTACT

    Gene models with high sequence identity

    1. AT5G28540.1 [+] show detail - heat shock protein 70 (Hsp 70) family protein
      E-value: 0; Score: 642.00

     

    InterPro Scan Digest

    Program Description E-value
    superfamily Actin-like ATPase domain 2.1e-66
    superfamily Heat shock protein 70kD (HSP70), peptide-bind 5e-66
    superfamily Heat shock protein 70kD (HSP70), C-terminal s 3.3e-30
    HMMPfam Heat shock protein 70 3.9e-169
    HMMPfam Glutamate dehydrogenase, NAD-specific 3.2e-23
    HMMPfam Glutamate dehydrogenase, NAD-specific 4.7e-61
    FPrintScan Heat shock protein Hsp70 3.4e-51
    FPrintScan Heat shock protein Hsp70 3.4e-51
    FPrintScan Heat shock protein Hsp70 3.4e-51
    FPrintScan Heat shock protein Hsp70 3.4e-51
    FPrintScan Heat shock protein Hsp70 3.4e-51
    Gene3D no description 3.2e-30
    Gene3D no description 1.4e-75
    HMMPanther HEAT SHOCK PROTEIN 70 (HSP70) 5.3e-207
    HMMPanther Heat shock protein Hsp70 5.3e-207
    PatternScan Heat shock protein 70, conserved site 0
    PatternScan Heat shock protein 70, conserved site 0
    Coil coiled-coil 0
    Coil coiled-coil 0

    See the detailed result >

    AT5G28540.1

    Model type
    Protein coding
    Short Description
    heat shock protein 70 (Hsp 70) family protein
    Curator Summary
    Encodes the luminal binding protein BiP, an ER-localized member of the HSP70 family. BiP is composed of an N-terminal ATP binding domain and a C-terminal domain that binds to hydrophobic patches on improperly/incompletely folded proteins in an ATP-dependent manner. Involved in polar nuclei fusion during proliferation of endosperm nuclei.
    Computational Description
    BIP1; FUNCTIONS IN: ATP binding; INVOLVED IN: protein folding, ER-associated protein catabolic process, response to heat, polar nucleus fusion; LOCATED IN: cell wall, plasma membrane, chloroplast, vacuole, endoplasmic reticulum lumen; EXPRESSED IN: 8 plant structures; EXPRESSED DURING: L mature pollen stage, M germinated pollen stage, seedling growth; CONTAINS InterPro DOMAIN/s: Heat shock protein 70, conserved site (InterPro:IPR018181), Heat shock protein Hsp70 (InterPro:IPR001023), Heat shock protein 70 (InterPro:IPR013126); BEST Arabidopsis thaliana protein match is: Heat shock protein 70 (Hsp 70) family protein (TAIR:AT5G42020.1); Has 36391 Blast hits to 35786 proteins in 4820 species: Archae - 162; Bacteria - 17493; Metazoa - 3988; Fungi - 1814; Plants - 1283; Viruses - 341; Other Eukaryotes - 11310 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR