K46348

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Phenotypes

Morphological phenotypes

Generation	Anatomical entity /structure development stage	Phenotypes
T1
	whole plant (PO:0000003)	decreased height (PATO:0000569) (Adult plant::Plant height::short)
	rosette leaf (PO:0000014)	decreased size (PATO:0000587) (Rosette leaf::Shape::small) increased width (PATO:0000600) (Rosette leaf::Shape::wide) dark green (PATO:0001249) (Rosette leaf::Color::dark)
	sporophyte vegetative stage (PO:0007134)	decreased speed (PATO:0000304) (Adult plant::Growth::slow)
	stem (PO:0009047)	increased thickness (PATO:0000591) (Stem::Shape::thick) abnormal (PATO:0000460) (Stem::Others)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K46348

Determination of introduced cDNA(s)

J023012D14 - Rice Full-length cDNAs - Original: J023012D14

full reading [+] show sequence

GCTCCCTCTC ATCATCATCA TCATCCTCCT CCTCCCTATC CCCTTCTCCC 
CCCTCTCTCT CTCTCTCCAC CCCGCGCGGC GGCCGCTTCC GATCCCTCTC 
CGACCGACCA AGCGCCGCCT CCAAGAATGG GGAGCTCGGA GAAGAACGGA 
ACTGCTTATG GCGAGTACAC CTATGCTGAA CTGGAGAGGG AGCAGTACTG 
GCCGTCTGAG AAGCTGAGGA TATCGATCAC CGGAGCTGGT GGTTTCATTG 
GATCCCACAT TGCTCGCCGT CTGAAGAGCG AGGGGCATTA CATCATCGCC 
TCCGACTGGA AGAAGAATGA GCACATGACT GAGGACATGT TCTGCCATGA 
GTTCCACCTT GTTGACCTTA GGGTCATGGA CAACTGCCTC AAGGTCACCA 
ACGGCGTCGA CCATGTGTTC AACCTTGCCG CTGATATGGG TGGTATGGGG 
TTCATTCAGT CCAACCACTC TGTGATCATG TACAACAACA CCATGATCAG 
TTTCAACATG CTCGAGGCTG CACGTATCAA TGGTGTGAAG AGGTTCTTCT 
ATGCCTCAAG TGCATGCATT TACCCTGAAT TCAAGCAGCT TGAAACTGAC 
GTTAGCCTGA AGGAATCTGA TGCCTGGCCT GCTGAGCCTC AAGATGCCTA 
TGGTTTGGAG AAGCTTGCAA CTGAGGAGCT CTGCAAGCAC TACACCAAGG 
ACTTTGGCAT TGAGTGCCGT GTTGGCCGCT TCCACAACAT ATATGGCCCC 
TTTGGAACAT GGAAAGGTGG CCGTGAGAAG GCACCAGCTG CATTCTGCAG 
GAAGGCTCAG ACTTCCACTG ACAGGTTTGA GATGTGGGGT GATGGCCTCC 
AGACCCGGTC CTTCACATTC ATAGATGAGT GTGTTGAGGG TGTTCTGAGG 
TTGACAAAGT CGGACTTCCG TGAGCCAGTG AACATTGGAA GCGATGAAAT 
GGTAAGCATG AACGAGATGG CTGAAATCAT TCTCAGCTTC GAGGATAGGG 
AGCTGCCCAT CCACCACATC CCTGGACCCG AGGGTGTCCG TGGCCGTAAC 
TCCGACAACA CCCTCATCAA GGAGAAGCTT GGCTGGGCAC CCACAATGAA 
GCTCAAGGAC GGGCTGAGGT TCACCTACTT CTGGATCAAG GAGCAGATAG 
AGAAGGAGAA GACCCAGGGC GTCGACATCG CGGGTTACGG CTCATCCAAG 
GTGGTGTCCA CCCAGGCCCC GGTTCAGCTG GGCTCCCTCC GTGCTGCCGA 
TGGCAAGGAG TAATTTCAAA GCTGCTACAC CATGAAGATC AATCAGCTCC 
TCTCAGCTTG ATCGTGGCCA TACCTTACTT CGCTACAGAT AGCTCACAGA 
AATGAATTCT TCTTGGAAAT CTTGCAACCT TTCAGAACAA CAACAGCCGT 
TCTTCCTGTC GTTATAACTC ATATGCAGTA CACCGGTTTC TTGTGTTTAA 
AAAGCTATAC TCGAGATTTC TTCCTCTTTT TTTTTTTCGC GTCGTGTTGG 
AAATTCTTCG TATGGATTAC ATTTTGTCAT ATGAACAATG AAACAATGGG 
AATGCTCTGC ATTACCTTTA TGAATAGATG ATGTGTTCTT CC

Gene models with high sequence identity

AT5G28840.2 [+] show detail - GDP-D-mannose 3',5'-epimerase
E-value: 0; Score: 711.00

InterPro Scan Digest

Program	Description	E-value
superfamily	NAD(P)-binding domain	8e-68
HMMPanther	DTDP-GLUCOSE 4-6-DEHYDRATASE	1.5e-134
HMMPanther	NAD DEPENDENT EPIMERASE/DEHYDRATASE	1.5e-134
HMMPfam	NAD-dependent epimerase/dehydratase	2e-45
Gene3D	NAD(P)-binding domain	4.3e-48

See the detailed result >

AT5G28840.2

Model type: Protein coding
Short Description: GDP-D-mannose 3',5'-epimerase
Curator Summary: Encodes a protein with GDP-D-mannose 3',5'-epimerase activity. The enzyme is involved in ascorbate biosynthesis. It catalyzes the conversion of GDP-D-mannose to GDP-L-galactose.
Computational Description: "GDP-D-mannose 3',5'-epimerase" (GME); CONTAINS InterPro DOMAIN/s: NAD-dependent epimerase/dehydratase (InterPro:IPR001509), NAD(P)-binding domain (InterPro:IPR016040); BEST Arabidopsis thaliana protein match is: NAD(P)-binding Rossmann-fold superfamily protein (TAIR:AT3G62830.2).
Link: InterPro Scan - TAIR